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Citations for Millipore Sigma :
701 - 750 of 10000+ citations for 5 Benzyloxy pyrimidine 2 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... we delivered the Iκ-kinase inhibitor [5-(p-Fluorophenyl)-2-ureido]thiophene-3-carboxamide (TPCA-1; Sigma-Aldrich CAS 507475-17-4 ...
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bioRxiv - Microbiology 2022Quote: ... zebrafish embryos were treated with 180 µM of 1-phenyl 2-thiourea (PTU) (Sigma-Aldrich, 103-65-5), to prevent pigmentation [25] ...
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bioRxiv - Cell Biology 2024Quote: ... 10 μL MTT (5 mg/ml tetrazolium salt 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide, Sigma-Aldrich) was added to each well and kept in a dark for 4 hours at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... β-estradiol was removed at day 5 from Type 2 medium and DMSO (Sigma, #D2650; final concentration = 0.1%) or 4-hydroxytamoxifen (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... asynchronously growing subconfluent KB2P1.21 or KB2P3.4 cells were labeled with 30μM thymidine analogue 5-chloro-2’-deoxyuridine (CIdU) (#C6891, Sigma-Aldrich) for 20’ ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoblots were carried out using the indicated antibodies: α-Tubulin (Sigma, B-5-1-2; T5168, 1:100,000), BLM (Bethyl ...
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bioRxiv - Cell Biology 2023Quote: ... Tissues were incubated overnight at room temperature with the B-5-1-2 monoclonal anti-α-tubulin (Sigma) and the anti-KNOLLE antibody (kind gift of G ...
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bioRxiv - Neuroscience 2023Quote: ... Larvae were incubated overnight from 4 dpf in 3 ml 10 mM 5-Bromo-2′-deoxyuridine (B5002, Sigma) with 1% DMSO for 17 hours ...
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bioRxiv - Neuroscience 2023Quote: ... the larvae were anaesthetized with 0.02% Tricaine for 5-10 minutes and immobilized in 1.5-2% low-melting point agarose (Sigma) in fish water ...
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bioRxiv - Cell Biology 2022Quote: ... reactions were incubated for 2 h on ice before quenching with 5 mM L-Cysteine (Millipore-Sigma, USA). The reactions were then adjusted to 1% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... reactions were incubated for 2 h on ice before quenching with 5 mM L-Cysteine (Millipore-Sigma, USA). The reactions were then adjusted to 1% (w/v ...
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bioRxiv - Immunology 2023Quote: ... 2% FCS and 50 μg/ml Lipopolysaccharide neutralizing agent Polymyxin B sulfate (cat # 1405-20-5; Sigma-Aldrich) for 30 min at 37°C while shaking by 750 rpm on a heating block ...
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bioRxiv - Systems Biology 2023Quote: ... Immunofluorescence studies were performed on 5 µm sections fixed in 2% paraformaldehyde (PFA; Millipore Sigma, St. Louisa, MO) for 5 minutes at room temperature (RT).
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bioRxiv - Microbiology 2023Quote: ... VirB or its mutant variants were incubated with 5 mM Tris(2-carboxy-ethyl) phosphine (TCEP; Sigma, USA) for 1 h at room temperature to fully reduce all cysteine residues ...
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bioRxiv - Developmental Biology 2023Quote: ... Tissues were hybridized overnight at room temperature with the B-5-1-2 monoclonal anti-α-tubulin (Sigma) and the anti-KNOLLE antibody65 (kind gift of G ...
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bioRxiv - Neuroscience 2023Quote: ... diluted in PBS + 5% NDS and in the second staining (2) 2B4 (1:200, Sigma Eldrich, Cat. # MAB5492), GFAP (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... Stock solutions of the NMDA receptor antagonist DL-2-amino-5-phosphonovaleric acid (DL-APV; #5282; Millipore Sigma), GABAA receptor antagonist bicuculline methiodide (#14343 ...
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bioRxiv - Immunology 2024Quote: One million of purified human CD4+ Tsen was activated with an anti-CD3 antibody coated-plate (0.5μg/ml) plus rhIL-2 (5 ng/ml; 11 011 456 001; Sigma). Forty-eight hours post-activation ...
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bioRxiv - Molecular Biology 2024Quote: ... pH 8.5 and reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich Cat No: C4706) for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... All media used for compound dilutions contained 20 mM glucose and 2% DMSO (Sigma Cat. #D2650-5×5ml).
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bioRxiv - Cancer Biology 2024Quote: ... The treatment group received 2 mg/ml Dox (BelaDox) dissolved in drinking water containing 5% sucrose (Sigma-Aldrich), to induce an in vivo knockdown ...
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bioRxiv - Cancer Biology 2024Quote: ... 3-(2-Methyl-5-nitro-imidazol-1-yl)-N-(2,2,2-trichloro-1-phenylamino-ethyl)-propionamide) (Sigma-Aldrich SML1503). Drugs were diluted in culture media at treatment.
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bioRxiv - Molecular Biology 2024Quote: ... Gene edition was induced in mESC lines by 5-day treatment with 2 μg/ml Doxycycline (Sigma, D9891). Cell lines expressing the sgRNA that yielded the best percentage of editing were used for further experiments ...
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bioRxiv - Microbiology 2024Quote: ... A 5% (w/v) mucin stock solution was prepared by dissolving mucin in 2 x DMEM (Sigma-Aldrich) by stirring at 37°C for 1h ...
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bioRxiv - Physiology 2024Quote: ... we used 10-5 M GDP-β-S (10-2 M stock solution dissolved in DMSO, Sigma-Aldrich) in SLR ...
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bioRxiv - Microbiology 2024Quote: ... After 24 h media/inhibitor was aspirated and replaced with 20 μl of 5 mg/mL 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma) and incubated at 37°C in 5% CO2 for 3 h ...
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bioRxiv - Neuroscience 2024Quote: ... juvenile female rats were injected once daily with FLX HCl (5 mg/kg/2 ml, ip, Sigma‒Aldrich) or with saline (2 ml/kg ...
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bioRxiv - Biophysics 2024Quote: ... The cells were incubated for 5 min in wash buffer containing 2× saline-sodium citrate buffer (SSC) (Sigma) and 20% formamide (Nakalai) ...
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bioRxiv - Cancer Biology 2024Quote: ... containing 10% FBS and 2 mM L-glutamine and supplemented with 5 g/L galactose (Sigma-Aldrich, #G5388) or 16mM 2-DG (MedChemExpress ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: The 5-HT2A antagonist (R)-(+)-α-(2,3-Dimethoxyphenyl)-1-[2-(4-fluorophenyl)ethyl]-4-piperinemethanol (M100907; Sigma Aldrich) at a dose of 0.5 mg/kg.
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bioRxiv - Cell Biology 2024Quote: ... mice received injections of tamoxifen (2 mg per day for either 5 or 7 days, Sigma-Aldrich, T5648) or an equivalent volume of corn oil as a vehicle control ...
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bioRxiv - Biochemistry 2024Quote: ... β-tubulin (1:2 000 in 5% skim milk/TBS-T, Cat. No. T8328, Sigma-Aldrich, Oakville, ON, CA). The signals were detected by secondary horseradish peroxidase conjugated antibodies (1:10 000 in 5% skim milk/TBS-T ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 150 mM NaCl and 5% (vol/vol) glycerol supplemented with 1mM Tris 2-carboxyethyl phosphine hydrochloride (TCEP, Sigma). Elution fractions were collected and evaluated by SDS-PAGE and relevant fractions with purified proteins were concentrated as appropriate for further studies.
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bioRxiv - Biochemistry 2020Quote: ... 2-2-2 trifluoroethanol (TFE) (Sigma, #T63002), and 200 mM dithiothreitol (DTT ...
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bioRxiv - Neuroscience 2021Quote: ... 2.2 mW.mm-2 for 5-10 ms) in control and in the presence of 1 µM strychnine (Sigma-Aldrich), 20 µM DNQX and 10 µM gabazine (BioTechne ...
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bioRxiv - Developmental Biology 2021Quote: ... fixed with 0.2% gluteraldehyde/4% PFA at room temperature and incubated overnight in hybridization buffer (5% Dextran sulphate, 2% blocking powder from Roche, 5X SSC, 0.1% TritonX, 0.1% CHAPS from Sigma Aldrich, 50% formamide ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 0.1% w/v Ferrozine (Disodium-4-[3-pyridin-2-yl-6-(4-sulfonatophenyl)-1,2,4-triazin-5-yl]benzosulfonate (Sigma-Aldrich) in dd ...
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bioRxiv - Physiology 2020Quote: The reduction of yellow tetrazolium salt 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Sigma-Aldrich, Germany) was used to measure cellular metabolic activity as a proxy for cell viability [19,22] ...
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bioRxiv - Biochemistry 2020Quote: ... After formation of the CAK-MAT1Δ219-THZ1 complex by incubation of 0.2 mg/mL CAK-MAT1Δ219 (approx. 2 μM) with 5 μM THZ1 (EMD Millipore) for 1.5 h at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... were added to 1 mL of a 0.1M solution of 5-norbornene-2-carboxylic acid in 0.1M phosphate buffer (pH 6.0) (all reagents purchased from Sigma-Aldrich). The solution was allowed to react at room temperature with intermittent vortexing ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 mM MgCl2 6 H2O and 5-Bromo-4-chloro-3-indolyl β-D-galactoside or X-gal (Sigma, B4252 ...
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bioRxiv - Cell Biology 2021Quote: ... 12-24hr embryo lysates were collected by homogenizing dechorionated embryos in a Dounce homogenizer with 100 μl of lysis buffer (60mM Tris pH7.5, 80mM NaCl, 6mM EDTA pH8.0, 2% Triton X-100, 5mM 1-Naphthyl potassium phosphate, 2mM PMSF, 1X Sigma Phosphatase Inhibitor II ...
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bioRxiv - Neuroscience 2020Quote: ... mice were pulsed with 100 µg per gram of body weight of 5-Ethynyl-2’-deoxyuridine (EdU) (Sigma-Aldrich) at 24 hours before sacrifice by injection intraperitoneally.
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bioRxiv - Microbiology 2021Quote: ... livers were finely minced with a sterile razor blade and digested overnight at 37°C in 40 mL 1×PBS containing 2% penicillin/streptomycin/amphotericin B (1×PBS −2% PSF) and 5 mL 0.5% clostridial collagenase solution (0.025 g of collagenase (Sigma, C0130) in 5 mL of dH20 for immediate use) ...
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bioRxiv - Cancer Biology 2021Quote: ... migrating/invading/transmigrating cells were incubated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; 5 mg/ml, 1/10 volume; Sigma-Aldrich) added to the culture medium at 37°C for 4h ...
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bioRxiv - Cell Biology 2021Quote: ... the mouse anti-alpha-tubulin B-5-1-2 was used at a dilution of 1:500 (Sigma, T5168). Polyclonal rabbit anti-TgCentrin1 (Fig ...
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bioRxiv - Biophysics 2020Quote: Zebrafish larvae at 5 days post-fertilization (dpf) were immobilized in 2% low melting point agarose (LMA) (Sigma-Aldrich) on microscope slides ...
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bioRxiv - Cell Biology 2020Quote: ... the hSCOs were either cultured in DM containing AEDs (valproic acid, 0.5 or 1 or 2 mM, Sigma, P4543; carbamazepine, 5 or 50 or 100 μM, Sigma, C4024 ...
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bioRxiv - Cell Biology 2020Quote: ... all the cell lines stably expressing inducible lenti vector were induced with 2-5 μg/ml of doxycycline (Sigma) for 24–96 h.
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bioRxiv - Neuroscience 2022Quote: ... Hippocampal neurons were cultured at a density of 5 × 104 cells.cm−2 on glass coverslips coated with 0.04% polyethyleneimine (Sigma, PEI) in Dulbecco⍰s Modified Eagle⍰s Medium (Gibco ...