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Citations for Millipore Sigma :
6451 - 6500 of 10000+ citations for Recombinant Human CYCS Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... The membrane with transferred protein was stained with Ponceau S stain (Sigma-Aldrich #P7170) to check the quality of transfer and protein load ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein bands were visualised by adding Immobilon Forte Western HRP substrate (Millipore #WBLUF0100) on a ChemiDoc XRS+ (BioRad).
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bioRxiv - Biochemistry 2024Quote: ... Protein fractions were collected and concentrated in a 10 kDa MWCO concentrator (Millipore Sigma), flash frozen in liquid nitrogen and stored at −80 °C.
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... a primary antibody against the HCoV-OC43 N protein (Millipore #MAB9013; diluted 1:500) was used ...
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bioRxiv - Immunology 2024Quote: ... Protein expression was induced by adding isopropyl β-D-thiogalactopyranoside (IPTG; 2.0 mM, Sigma). Incubation was continued for another 4h at 37°C in a shaking incubator ...
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bioRxiv - Immunology 2024Quote: ... The first two immunizations included 100µg/100µl OVA protein (Sigma Aldrich, 9006-59-1) in complete Freund’s adjuvant (CFA ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were separated on 5.5% polyacrylamide (PAA) gel and transferred on PVDF membrane (Millipore) using semi-dry blotting for 60 min ...
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bioRxiv - Neuroscience 2024Quote: ... cell lysates were harvested from hMGLs using RIPA protein lysis buffer (#20-188; Millipore) containing 1X protease and 1X phosphatase inhibitors (#PI78443 ...
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bioRxiv - Neuroscience 2024Quote: ... and immunoreactive proteins were visualized using Immobilon® Forte Western HRP Substrate (Merck Millipore) at the Imager Chemi Premium (VWR International) ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were separated by SDS-PAGE and transferred to a PVDF membrane (Millipore). The blots were blocked with 5% non-fat milk ...
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bioRxiv - Biochemistry 2024Quote: ... The final concentration of the protein as estimated from the BCA test (Sigma Aldrich) was 15.4 mg/ml.
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bioRxiv - Biochemistry 2024Quote: ... The proteins separated by SDS-PAGE were transferred to PVDF membranes (Millipore, Cat#PVH00010). After blocking with 5% (w/v ...
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bioRxiv - Systems Biology 2024Quote: ... followed by filtration using 96-Well protein precipitation filter plate (Sigma-Aldrich, 55263-U). Retention index (RI ...
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bioRxiv - Pathology 2024Quote: Protein samples were extracted using radioimmunoprecipitation buffer containing inhibitors of proteases (cOmplete, Sigma-Aldrich) and phosphatases (PhosSTOP ...
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bioRxiv - Cell Biology 2024Quote: Protein content in mitochondria-enriched fractions were estimated using Bradford Assay reagent (Sigma-Aldrich) followed by solubilisation as previously reported by Rawat et al21 ...
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bioRxiv - Genetics 2024Quote: ... proteins on the SDS-PAGE were transferred to a PVDF membrane (Millipore, Billerica, MA) at 100V for 3 hours at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Purified His-tagged proteins were concentrated and desalted using Amicon centrifugal filters (Millipore, UFC500396) in TBS buffer and stored in storage buffer (30 mM Tris–HCl ...
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bioRxiv - Plant Biology 2024Quote: ... The eluted protein was further concentrated using a spin concentrator (10 kDa Amicon, Millipore) and rebuffered in 20 mM HEPES pH 7.5 ...
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bioRxiv - Plant Biology 2024Quote: ... Protein concentrations were determined by using a SpectraMax QuickDrop and bovine serum albumin (Sigma) as the reference standards for analysis.
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bioRxiv - Neuroscience 2024Quote: ... and then proteins were transferred to an Immobilon PVDF membrane (EMD Millipore, Temecula, CA) at 25V for 90 minutes ...
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bioRxiv - Genetics 2021Quote: ... Then human 293T cells were transduced with lentiviral particles of plkoSCR (Mission plko.1 puro; SHC002) or shCNBP human (Mission plko.1 puro TRCN0000311158, SIGMA-ALDRICH) at a MOI=5 for 72h ...
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bioRxiv - Pathology 2021Quote: Hydrogels (n=6/time point) were immersed in a solution of fluorescein-conjugated human IgG (IgG-FITC, final concentration 1 mg/mL) (Sigma-Aldrich) in TBS containing 0.1% (v/v ...
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bioRxiv - Developmental Biology 2021Quote: ... Cumulus-oocyte complexes were collected from the oviduct of wild-type female C57BL/6 mice at 16 h after ovulatory human chorionic gonadotropin (hCG, Sigma-Aldrich) injection and placed in a 50 µL drop of TYH medium that was then covered with mineral oil (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... and 1x non-essential amino acid) and 50 ng/mL of human macrophage colony-stimulating factor (M-CSF, Millipore, Temecula, CA) for 5-6 days at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... Parasites were maintained in vitro in human O+ erythrocytes at 4% haematocrit in RPMI 1640 medium supplemented with 25mM HEPES (Sigma-Aldrich), 0.25% sodium bicarbonate ...
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bioRxiv - Molecular Biology 2022Quote: Human HEK293T cell line was grown in Dulbecco’s Modified Eagle’s Medium containing high glucose with HEPES modification (Sigma-Aldrich®, D6171), supplemented with 10% fetal bovine serum (PAN Biotech ...
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bioRxiv - Molecular Biology 2021Quote: Murine or human differentiated brown adipocytes were fixed with 4% PFA and stained with 0.3% Oil Red O solution (Sigma-Aldrich, O0625). The images were observed under a Nikon microscope.
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bioRxiv - Biochemistry 2020Quote: ... The following compounds were selected from the “in-human” collection docking screen and purchased from different vendors: Pterin (Sigma-Aldrich, P1132), Verdiperstat (MedChem Express ...
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bioRxiv - Immunology 2021Quote: ... the plates were washed six times and incubated with 100 μL/well horseradish peroxidase (HRP)-conjugated goat anti-human IgG (Fc-specific, Sigma Aldrich) diluted 1/5000 in PTA buffer ...
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bioRxiv - Immunology 2019Quote: Blood derived from healthy human volunteers was collected into citrate tubes (Becton Dickinson) and layered into Ficoll-Paque Plus (Sigma-Aldrich). Samples were centrifuged at 1440 rpm in a swinging bucket centrifuge for 20 min at 23°C without braking ...
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bioRxiv - Biochemistry 2019Quote: ... Cohn fraction IV (Product # G3637) and human whole serum (sterile-filtered from male AB plasma, Product # H4522) were purchased from Sigma-Aldrich, St ...
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bioRxiv - Physiology 2019Quote: ... following which they were treated with media containing 20% human serum (fed or fasted) and 1 µM puromycin (Merck Millipore Limited) for a further 4 h ...
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bioRxiv - Immunology 2019Quote: ... Cytokine levels in restimulated T cell cultures and SLE patient plasma samples was measured using Milliplex® MAG Human Cytokine/Chemokine Immunoassays (Millipore).
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bioRxiv - Neuroscience 2019Quote: ... transverse paraffin sections of spinal cord mounted on positive charged glass slides were processed for double immunohistochemistry with specific antibodies for human neural stem cells (Sigma-Aldrich) and examined under a fluorescence microscope ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed four times with PBST and 100 μl/well of goat anti-human immunoglobulins-peroxidase IgG (FC specific; Sigma-Aldrich), IgM (μ-chain specific ...
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bioRxiv - Immunology 2019Quote: ... PerioPaper strips were centrifuged at 3000 × g for 15 min at 4°C in PBS elution for the dosage of the tissue destruction and bone resorption markers by Multiplex (Human MAP, Millipore, USA).
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bioRxiv - Biochemistry 2019Quote: ... and eluted by competitive elution in Flag Wash Buffer supplemented to 200 ug/mL Flag peptide and 250 ug/mL human insulin as a carrier (Sigma-Aldrich). The protein was then passed over a 2 mL amylose column (NEB ...
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bioRxiv - Neuroscience 2019Quote: ... plane through the human temporo-basal neocortex and post-fixed for 30 to 60 min in 0.5 or 1% osmium tetroxide (Sigma, Munich, Germany) diluted in PB-buffered sucrose (300 mOsm ...
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bioRxiv - Systems Biology 2019Quote: Human serum (Cat. No. H6914, from a male AB clotted whole blood, USA origin, sterile-filtered) was obtained from Sigma-Aldrich, St ...
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bioRxiv - Immunology 2019Quote: ... The MILLIPLEX®MAP Human Isotyping Magnetic Bead Panel-Isotyping Multiplex Assay (HGAMMAG-301K-06, Merck Millipore Corporation, Billerica, MA, USA) was used to measure IgG1 ...
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bioRxiv - Developmental Biology 2020Quote: Female 7-12-week-old FVB/NJ mice were superovulated by intraperitoneal injection of 5 IU pregnant mare serum gonadotropin (PSMG) (ProSpec HOR-272) followed by 5 IU human chorionic gonadotropin (hCG) (Sigma CG10) 48 hours later.
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bioRxiv - Neuroscience 2020Quote: Soluble and insoluble human brain samples and mouse brain samples were homogenized in PBS with a protease inhibitor cocktail (catalog no. 11873580001; Sigma Aldrich) with 3.2 mm stainless steel beads ...
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bioRxiv - Cell Biology 2021Quote: ... in the presence of HODHBt or DMSO control and cultured in complete RPMI 1640 medium containing 10% human AB serum (Sigma-Aldrich). As a control ...
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[18F]DCP, First Generation PET Radiotracer for Diagnosis of Radiation Resistant Head and Neck CancerbioRxiv - Cancer Biology 2020Quote: The ex vivo serum stability of the radiotracer [18F]DCP was performed following previously published methods (55–57) and human serum (Sigma Aldrich). [18F]DCP (~1-2 mCi ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lines stably expressing shRNA to human FBXO7 were generated as described previously (33-35) and selected with 2 µg/mL puromycin (Sigma-Aldrich). To vary glucose concentration ...
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bioRxiv - Biochemistry 2021Quote: ... All wells were then incubated with 3,000-fold diluted goat anti-human IgG-alkaline phosphatase conjugate (Sigma-Aldrich, St. Louis, MO) for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: Free floating mouse brain sections were incubated with 300 nanograms of active human cathepsin-S (SRP0292, Sigma-Aldrich, St. Louis, MO), in activation buffer containing 1.8 mM DTT ...
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bioRxiv - Microbiology 2020Quote: ... One hundred microliters of secondary antibody (alkaline phosphatase-conjugated goat anti-rabbit IgG or goat anti-human IgG, both from Sigma-Aldrich) diluted 1:2,000 in 1% nonfat milk-PBS were then added to each well and the plates incubated for additional 3 h at room temperature before repeating the washing step ...
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bioRxiv - Systems Biology 2021Quote: ... purified LC and naïve CD4+ T cells or TRMs were co-cultured in human serum supplemented media (RPMI, Gibco, UK, 10% human serum, Sigma, UK, 100 IU/ml penicillin and 100 mg/ml streptomycin, Sigma, UK) at a 1:50 ratio for 5-days at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... 200 μl of bacterial suspension was added to 400 μl of pre-warmed at 37°C human sera (Sigma-Aldrich S7023), and the mixture was incubated for 2h at 37°C ...