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Citations for Millipore Sigma :
6351 - 6400 of 10000+ citations for Recombinant Human CYCS Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Proteins were reduced with the addition of 3 μL of 250 mM dithiothreitol (Sigma) at 37°C for 45 min and alkylated with 4.5 μL of 500 mM chloroacetamide for 30 min at room temperature in the dark ...
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bioRxiv - Immunology 2023Quote: ... The boiled proteins were resolved by SDS-PAGE and transferred to nitrocellulose membranes (Millipore). The membranes were blocked in TBST (Tris-buffered saline ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 mM TCEP and mixed with 1:5000 SYPRO Orange Protein Gel Stain (Sigma). 50 μL of assay mixture was dispensed per well in a white bottom qPCR plate and each condition was performed in technical triplicate ...
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bioRxiv - Pathology 2023Quote: ... and proteins were electrophoretically transferred onto methanol-activated polyvinylidene fluoride (PVDF) microporous membranes (Millipore). Membranes were blocked for one hour with 5% bovine serum albumin (Sigma ...
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bioRxiv - Immunology 2023Quote: ... the proteins were transferred onto PVDF membrane (Merck Millipore, cat. no. IPVH00010, Jerusalem, Israel). Prior to antibody incubation ...
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bioRxiv - Cell Biology 2023Quote: ... SDS-PAGE and protein transfer to polyvinylidene difluoride (PVDF) membrane (Immobilon-P, Merck Millipore) was performed with the NuPAGE Novex and XCell II Blot Module systems (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated with antibodies against OL markers proteins including Olig2 (MABN50, 1:500 Millipore, CA ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were concentrated by ultrafiltration on 10K Amicon® Ultra Centrifugal Filters (Merck Millipore), aliquoted ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were then reduced by 10 mM tris(2-carboxyethyl)phosphine (TCEP, Millipore Sigma) for 30 min at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: Total proteins were extracted from organoid cell pellets using 1X RIPA buffer (Sigma Aldrich) supplemented with 1X Halt™ protease and phosphatase inhibitor cocktail (Pierce) ...
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bioRxiv - Microbiology 2023Quote: ... Sized proteins were concentrated to >50 mg/ml using 10 kDa MWCO concentrators (Millipore), flash frozen on liquid nitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... The eluted protein was concentrated using Amicon Ultra-15 Centrifugal Filter Units (Millipore-Sigma) centrifuged at 4,000 x g to no more than 3 ml ...
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bioRxiv - Molecular Biology 2023Quote: ... The eluted protein was concentrated using Amicon Ultra-15 Centrifugal Filter Units (Millipore-Sigma) centrifuged at 4,000 x g to no more than 3 ml ...
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bioRxiv - Immunology 2023Quote: ... protein bands on the blots were developed with chromogenic substrate SigmaFast BCIP/NBT (Sigma).
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bioRxiv - Biophysics 2023Quote: ... Resolved proteins were then electrophoretically transferred onto polyvinylidene difluoride (PVDF) membranes (Merck Millipore, USA) at 90 V for 2.5 h ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was discarded and the protein pellet was washed using guanidine hydrochloride (Millipore, #1.04220.0100 ...
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bioRxiv - Biochemistry 2023Quote: ... the proteins were filtrated using a 96-well filter plate (Millipore, Burlington, MA, USA) to remove precipitated protein and the A280 was remeasured before starting the binding assay ...
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bioRxiv - Cancer Biology 2023Quote: ... and the protein was then transferred to 0.4 μm polyvinylidene fluoride (PVDF, Merck Millipore) membrane ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were transferred onto a PVDF membrane (Immobilon-P, Merck Millipore, Massachusetts, USA) and the membranes were blocked with PBS containing 5% non-fat dry milk for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by 10% SDS-PAGE gel and transferred onto PVDF membranes (Millipore). After blocked in 5% skim milk for 2 hours ...
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bioRxiv - Neuroscience 2023Quote: Total protein from cortical organoids and HLCs were isolated using RIPA buffer (Sigma-Aldrich) consisting of protease and phosphatase inhibitors (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... Protein lysates were collected from cells using ice cold RIPA buffer (R2078, Sigma Aldrich) with HaltTM 100x protease inhibitor cocktail added (78440 ...
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bioRxiv - Neuroscience 2023Quote: ... Protein concentrations were normalized with Milliplex® MAP Assay Buffer (EMD Millipore, Billerica, MA) to 6 μg protein per 12.5 μL for cytokine analysis and 1 μg protein per 12.5 μL for the MAPK pathway analysis ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were eluted using 10 mM glutathione or 0.5 mg/mL 3XFLAG peptide (Sigma) in Lysis Buffer with 2 mM DDM.
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bioRxiv - Plant Biology 2023Quote: ... Expression of fusion proteins was verified by immunoblotting using either mouse anti-LexA (Sigma) or rat anti-hemagglutinin (HA ...
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bioRxiv - Biochemistry 2023Quote: ... Cleaved proteins in the supernatant were concentrated with a 100K MWCO concentrator (EMD Millipore) to 500 µl and purified via size exclusion chromatography on a TSKgel G4000SWXL column (TOSOH Bioscience ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant containing cleaved proteins was concentrated using a 30kDa MWCO concentrator (EMD Millipore) to 1mL ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were separated and transferred to Immobilon-P PVDF membrane (0.45 μm, Millipore Sigma). The membrane was blocked with 5% bovine serum albumin for 1 h and incubated overnight at 4°C with various antibodies (1:500 dilution for the PIKfyve antibody and 1:1000 dilution for all other antibodies) ...
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bioRxiv - Biochemistry 2023Quote: ... Protein expression was performed in the presence of 3.5 mM valproic acid (Merck Millipore) and 10 μM kifunensine (Biomol) ...
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bioRxiv - Genomics 2022Quote: ... Proteins were transferred to a PVDF membrane (Millipore; Immobilon-FL, 0.45 mm pore size) in transfer buffer (25 mM tris ...
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bioRxiv - Biophysics 2023Quote: ... then the proteins were stained with 0.22% Coumassie brilliant blue G-250 (Sigma, USA) diluted in the solution F ...
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bioRxiv - Bioengineering 2023Quote: ... then resuspended in 100 µL protein storage buffer: 4 M urea (Sigma-Aldrich, U5128), 1 % SDS (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were probed with 1:2000 chicken anti-LDL primary antibody (#GW20089F, Sigma-Aldrich) diluted in PBS-T overnight at 4°C and detected with 1:2000 HRP-conjugated goat anti-chicken secondary antibody (#A16054 ...
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bioRxiv - Cell Biology 2022Quote: ... For Chemiluminescence westerns: The proteins were electro-transferred to PVDF membranes (0.45 μm, Millipore), and then the total protein was stained using Ponceau (Thermo Fisher Scientific) ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... Protein transfer was performed on methanol-activated Immobilon®-FL PVDF membrane (Sigma-Aldrich). Membrane was blocked for 1h in 5% BSA (bovine serum albumin ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µg of pure protein emulsified in Freund’s incomplete adjuvant (Sigma Aldrich, Taufkirchen, Germany) was injected subcutaneously to six weeks old female NMRI mice (Charles River Laboratories ...
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bioRxiv - Immunology 2023Quote: ... proteins were transferred to a nitrocellulose membrane and stained with Ponceau S (Sigma Aldrich) to monitor protein loading ...
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bioRxiv - Biochemistry 2023Quote: ... Expression of chaperone protein was induced with 1 μg/ml tetracycline (Sigma-Aldrich, T7660).
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bioRxiv - Biochemistry 2023Quote: ... Unbound protein was then concentrated in a 30 kDa MWCO Amicon concentrator (Sigma Millipore) and then loaded onto a 24 mL Superdex 200 10/300 GL (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... Unbound protein was then concentrated in a 30 kDa MWCO Amicon concentrator (Sigma Millipore) and then loaded onto a 24 mL Superdex 200 10/300 GL (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2023Quote: ... protein expression was induced by adding 0.5 mM isopropyl β-Dthiogalactopyranoside (IPTG; Sigma Aldrich). After 20h incubation at 15°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Biotinylated proteins were detected with a streptavidin-HRP conjugate (Millipore-Sigma, Cat#: OR03L-200UG), used at 1:2,000 dilution (see Reagent Table).
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bioRxiv - Biochemistry 2023Quote: ... the protein was concentrated using a centrifugal filter (Amicon Ultra, MWCO 30 kDa, Sigma) to 41.46 mg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... The protein was concentrated with an Amicon Ultra-4 (Merck Millipore, MWCO 3 K) to a concentration of 726 µM ...
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bioRxiv - Developmental Biology 2023Quote: ... Then proteins were transferred onto a 0.45-μm polyvinylidene fluoride membrane (Sigma Aldrich, IEVH00005) and the membranes were blocked in TBST containing 5% skimmed milk for 1 h at room temperature and then incubated with the indicated primary antibodies overnight at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... GFP-V5 or GFP-FLAG fusion proteins using OptiMem and Fugene HD (Sigma-Aldrich). Forty-eight hours later ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were eluted twice with 2 μg/μl V5 peptide in water (Sigma-Aldrich) or 1 μg/μl 3x FLAG peptide (ApexBio ...
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bioRxiv - Cancer Biology 2023Quote: ... Separated proteins were then transferred to Immobilon®-P PVDF Membranes (Milipore Sigma #ISEQ00010) using the BioRad Transblot SD Semi-dry Transfer Cell as per manufacturer’s recommendations.
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bioRxiv - Microbiology 2023Quote: ... Proteins were reduced for 10 mins at 60°C with 10 mM dithiothreitol (Sigma) in 25 mM ammonium bicarbonate and then alkylated with 55 mM iodoacetamide (Sigma ...