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Citations for Millipore Sigma :
6401 - 6450 of 10000+ citations for Recombinant Human CYCS Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The resolved proteins were transferred to an Immobilon-P polyvinylidene difluoride (PVDF) membrane (Millipore), and the membranes were incubated with Tris-buffered saline with Tween 20 (TBST ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were similarly incubated with HA binding protein (HABP) (EMD Millipore 385911, Burlington, MA), then detected with an appropriate fluorescent secondary antibody for 1 h at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins separated by SDS-PAGE were transferred to PVDF membranes (Millipore, Cat#PVH00010). After blocking with 5% (w/v ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were then wet transferred to a polyvinylidene difluoride (PVDF) membrane (IPVH00010, Sigma Aldrich). The membrane was incubated in blocking buffer consisting of 5% milk in PBS plus 0.1% Tween-20 ...
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bioRxiv - Biochemistry 2023Quote: ... The interaction of the proteins was quantified after adding the substrate phenylenediaminedihydrochloride (Sigma Aldrich) plus H2O2 by measuring the resulting absorbance at 492 nm in the Multiskan SkyHigh Spectrophotometer (ThermoFisher Sientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were concentrated using AmiconUltra (M.W. 3,000 Da; Sigma-Aldrich, St. Louis, MO, USA) and stored at −80 °C before use.
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bioRxiv - Biochemistry 2023Quote: ... Flag-tagged protein was purified using 20µl anti-Flag® M2 magnetic beads (Sigma) per plate ...
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bioRxiv - Plant Biology 2023Quote: Proteins were transferred to a PVDF membrane (Immobilon-P, Millipore Corporation, Billerica, MA, USA) via semi-dry Western blotting ...
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bioRxiv - Immunology 2023Quote: ... and protein complexes were eluted with 150 mg/ml of 3X FLAG peptide (Sigma). Samples were diluted in 2X SDS sample buffer and analyzed with SDS–PAGE according to standard procedures ...
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bioRxiv - Cell Biology 2023Quote: ... the protein was concentrated using a 50 kDa cut-off Amicon filter (Merck Millipore) and loaded onto a pre-equilibrated Superdex 200 Increase 10/300 GL column (Cytiva) ...
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bioRxiv - Neuroscience 2023Quote: ... Equal amounts of proteins were incubated overnight with the anti-FLAG M2 (Sigma M8823) or anti-HA (Pierce #88836 ...
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bioRxiv - Microbiology 2023Quote: ... followed by protein precipitation after the addition of trichloroacetic acid (TCA, Sigma-Aldrich, 91228), (v:v ...
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bioRxiv - Molecular Biology 2023Quote: RIP was performed using a Magnetic RIP RNA-Binding Protein Immunoprecipitation (Millipore, 17-700) by the manufacturer’s guidelines ...
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Dynamics of the epigenetic landscape during development and in response to drought stress in sorghumbioRxiv - Plant Biology 2023Quote: ... H2A.Z (preparation using antigen “GKGLLAAKTTAAKSAEKDKGKKAPV”) were incubated with Protein A agarose (16-157, Millipore) before mixed with sonicated chromatin ...
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bioRxiv - Pathology 2023Quote: Protein was extracted by 1x RIPA lysis buffer (10x, stock solution, EMD Millipore, MA) from mouse stomach tissue ...
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bioRxiv - Genetics 2023Quote: ... 20 μL Magna ChIP™ Protein A+G Magnetic Beads (Sigma-Aldrich 16-663) was washed four times with 1X DPBS supplemented with 5 mg/mL BSA and subsequently crosslinked to 5 μg antibody (Anti-FOXA1 antibody ...
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bioRxiv - Genetics 2023Quote: ... and the supernatants were precleared with 10 μL of Protein A-coated agarose (Millipore). The precleared supernatants were then divided into two equal parts and incubated with 20 μL 14-3-3 polyclonal antibody and IgG overnight at 4 °C ...
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bioRxiv - Genomics 2023Quote: ... the supernatants were collected and protein concentration was measured by Bradford assay (Sigma-Aldrich). Extracts were boiled in presence of SDS loading buffer and analyzed by Western blotting.
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bioRxiv - Molecular Biology 2023Quote: ... separated by SDS-PAGE and proteins transferred to polyvinylidene difluoride membrane (Immobilon-P, Millipore). Membranes were blocked in 5% milk in PBS/ 0.1% Tween-20 ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein concentration was measured using a Bradford assay (Sigma-Aldrich/Millipore B6816-500ML) as per manufacturer’s instructions in optically clear 96-well plate (VWR 62402-933 ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein concentration was measured using a Bradford assay (Sigma-Aldrich/Millipore B6816-500ML) as per manufacturer’s instructions in optically clear 96-well plate (VWR 62402-933 ...
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bioRxiv - Cell Biology 2023Quote: ... the protein was concentrated using a 50 kDa cut-off Amicon filter (Merck Millipore) and loaded onto a pre-equilibrated Superdex 200 Increase 10/300 GL column (Cytiva) ...
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bioRxiv - Cell Biology 2023Quote: ... protein lysates were extracted from BMDMs using Radioimmunoprecipitation assay (RIPA) buffer (Sigma-Aldrich, R0278) complimented with protease inhibitor (Roche Diagnostics ...
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bioRxiv - Neuroscience 2023Quote: ... PLP (Anti-Myelin Proteolipid Protein Antibody, CT, clone PLPC1 MAB388 Sigma-Aldrich, 1:200), MHCII (Dako 1:50) ...
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bioRxiv - Biophysics 2023Quote: ... GST-tagged Elf1 protein was expressed in Escherichia coli strain Rosetta 2(DE3) (Novagen) and purified by Glutathione Sepharose 4 Fast Flow resin (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were resolved in 10% SDS/PAGE and transferred to nitrocellulose membranes (Sigma Aldrich). Membranes were probed for 2 h at RT (or overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by SDS-PAGE and transferred to polyvinyl difluoride (PVDF) membranes (Millipore) according to standard protocols ...
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bioRxiv - Developmental Biology 2023Quote: ... supernatant containing protein suspension was collected and quantified with Bradford reagent (Sigma, B6916-500ML) at the spectrophotometer ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... protein levels was done using heparin affinity columns (Sigma-Aldrich, St. Louis, MO, USA) and Western blot analysis as previously described [37 ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to a polyvinylidene difluoride immobilon TM-P membrane (Millipore, Burlington, MA) overnight at 4°C and blocked in 5% nonfat powdered milk in TBS with Tween 20 (pH 7.6 ...
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bioRxiv - Plant Biology 2024Quote: ... Purified proteins were concentrated using 10 kDa ultra-centrifugal filter Amicon (Merck Millipore, Germany). The quality and quantity of proteins were analyzed by SDS-protein gel electrophoresis (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... was subjected to affinity purification using S-protein-agarose (Merck Millipore, Burlington, MA, U.S.A.) for 1– 2 h and washed 4 times ...
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bioRxiv - Microbiology 2024Quote: ... purified RSP protein was mixed with Biotin-NHS (Biotinamidocaproate N-hydroxysuccinimide ester, Sigma Aldrich) and three rounds of MACS (with RSP concentrations of 100 nM (the first one ...
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bioRxiv - Biophysics 2024Quote: ... Protein bands were transferred by tank transfer onto a polyvinylidene difluoride (PVDF, EMD Millipore) membrane in transfer buffer (25 mM Tris pH 8.3 ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were reduced with 20 mM DL-Dithiothreitol (DTT; BioUltra, for molecular biology, Sigma) at 56°C for 30 minutes ...
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bioRxiv - Microbiology 2024Quote: ... Protein precipitates were solubilized in 6M urea and subsequently quantified using BCA kit (Sigma). Ten micrograms of the protein samples were reduced with 10mM dithiothreitol (DTT ...
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bioRxiv - Biochemistry 2024Quote: ... 100 mM Tris pH 8.5 with 800 ng of yeast enolase protein (Sigma-Aldrich) added as an internal protein control ...
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bioRxiv - Neuroscience 2024Quote: ... The Syt1 and Syt7 proteins were further treated with Benzonase (Millipore Sigma, Burlington, MA) at room temperature for 1 hr with Syt1 additionally being run through ion exchange (Mono S ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were eluted using 44 μl FLAG-peptides (200 μg/ml; Merck/Sigma Aldrich) in 1x TBS ...
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bioRxiv - Biophysics 2024Quote: ... Protein was initially purified using affinity chromatography using HIS-Select Cobalt Affinity Gel (Sigma) on a gravity flow column ...
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bioRxiv - Biophysics 2024Quote: ... The final concentration of the protein as estimated from the BCA test (Sigma Aldrich) was 8.4 mg/ml.
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bioRxiv - Cell Biology 2024Quote: ... Solubilized protein was added to pre-equilibrated anti-FLAG agarose affinity resin (Sigma, A2220) and incubated for 90min at 4°C on a rotating wheel ...
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bioRxiv - Cell Biology 2024Quote: ... Cleaved proteins in the supernatant were concentrated with a 100K (MWCO) concentrator (EMD Millipore) to 500 μL and purified via SEC on a TSKgel G4000SWXL column (TOSOH Bioscience ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were incubated with 20μl α-V5 or α-FLAG M2 resins (Sigma-Aldrich) for 4 hours at 4°C to pull down CLK or CK1α ...
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bioRxiv - Biochemistry 2024Quote: ... 100 mM Tris pH 8.5 with 800 ng of yeast enolase protein (Sigma-Aldrich) added as an internal protein control ...
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bioRxiv - Bioengineering 2024Quote: ... After dialysis the protein was concentrated through Amicon centrifugal filters (Millipore, Burlington, MA, USA) with a molecular weight cutoff of 3 kDa ...
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bioRxiv - Biophysics 2024Quote: ... Heterologous protein expression was then initiated with 0.02% w/v L-Arabinose (Sigma A3256) overnight at 30 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... proteins were resolved by SDS-PAGE and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore). Immunoblots were developed using Western Lightning Chemiluminescence Reagent Plus (Advansta ...