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Citations for Millipore Sigma :
6701 - 6750 of 10000+ citations for Recombinant Human CYCS Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... The native proteins without signal peptides were expressed in BL21(DE3) Gold competent cells (Novagen). The gene encoding the LtgA deletion mutant lacking the alpha helix 503(ATAREIAGKIGMD)513 was chemically synthesized by ProteoGenix ...
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bioRxiv - Molecular Biology 2019Quote: ... Thrombin was removed from protein-containing fractions with 40 μL p-aminobenzamidine beads (Sigma #A7155) washed twice with 400 uL 137 mM NaCl ...
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bioRxiv - Molecular Biology 2019Quote: ... protein production was induced with 0.4 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (Sigma #I6758), and the culture was incubated at 37°C and 250 rpm for 4 hr ...
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bioRxiv - Microbiology 2019Quote: ... Protein A/G Plus agarose beads were blocked with bovine t-RNA (1mg/mL; Sigma) for 2h in modified-RIPA buffer ...
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bioRxiv - Genetics 2020Quote: ... Chromatin-bound antibodies were recovered with 30μl Protein A/G magnetic beads (Millipore 16-663). After reverse crosslinking ...
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bioRxiv - Microbiology 2019Quote: WT ppr-SiiA protein and mutant variants were produced using freshly transformed BL21(DE3) (Novagen) cells ...
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bioRxiv - Molecular Biology 2020Quote: ... The immunoprecipitates were purified using Protein G Immunoprecipitation Kit (Cat. No. IP50, Sigma-Aldrich Inc.) and were separated on SDS-PAGE for western blotting using monoclonal antibodies against T18 as described above ...
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bioRxiv - Neuroscience 2019Quote: ... Nonspecific protein binding sites were blocked with 4% normal donkey serum (NDS; Sigma-Aldrich, D9663) with 1% bovine serum albumin (BSA ...
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bioRxiv - Immunology 2020Quote: ... The protein pellet was resuspended in 100µl of 50mM Triethylammonium bicarbonate (TEAB, Sigma-Aldrich, USA). Trypsin (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... The protein pellet was resuspended in 100µl of 50mM Triethylammonium bicarbonate (TEAB, Sigma-Aldrich, USA). Trypsin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... FLAG-tagged proteins were eluted using FIB containing 0.1 mg/mL FLAG peptide (Sigma Aldrich). All immunoprecipitations were performed on three independent biological replicates.
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bioRxiv - Bioengineering 2019Quote: Proteins were isolated from cells cultured on films or scaffolds in RIPA buffer (Sigma-Aldrich), supplemented with cOmplete™ ...
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bioRxiv - Cancer Biology 2021Quote: ... All FLAG-tagged proteins were purified with anti-FLAG® M2 Affinity Gel (Sigma-Aldrich) in a native condition using the FLAG peptide elution ...
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bioRxiv - Developmental Biology 2021Quote: ... The protein was then transferred from the gel to a PVDF membrane (Immobilon-P, Millipore) in transfer buffer (20% methanol ...
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bioRxiv - Biochemistry 2021Quote: ... The bacterial pellet was lysed in 2 mL 1× BugBuster™ protein extraction reagent (Novagen), incubated by slow shaking for 20 min ...
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bioRxiv - Plant Biology 2020Quote: ... and then the proteins were immunoprecipitated with anti-FLAG M2 affinity gel (Sigma-Aldrich, M8823), anti-GFP mAb-magnetic agarose (MBL ...
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bioRxiv - Microbiology 2021Quote: Vero cells were transfected with spike protein expression constructs using the GeneJuice transfection reagent (Novagen). The indicated S-protein expression constructs (1 µg ...
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bioRxiv - Neuroscience 2021Quote: ... Puromycin-labeled proteins were identified using the mouse monoclonal antibody (1:10,000, Millipore, catalog MABE343). Protein synthesis levels were determined by analyzing total lane density from 10 kDa to 250 kDa ...
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bioRxiv - Neuroscience 2020Quote: ... Cellular and histone proteins were precipitated by incubating samples with 6M ammonium acetate (Sigma; A1542) on ice ...
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bioRxiv - Cell Biology 2020Quote: ... Viral supernatants were filtered through a 0.45 μm (low protein binding) filter unit (EMD Millipore). The filtered retroviral supernatant was then concentrated 20-fold using an Amicon Ultra-15 concentrator (EMD Millipore ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were transferred to a nitrocellulose membrane that was blocked with 5% skim milk (Sigma) in PBS overnight and incubated with rabbit antiserum against ACS (1:1000 ...
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bioRxiv - Biophysics 2020Quote: ... The protein solution was then concentrated using an Amicon Ultra 15 Ultracel-10K device (Millipore). Samples were desalted using a Millipore Ziptip C18 ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were concentrated to 12 mg/mL using an Amicon Ultra Centrifugal Filter (Merck-Millipore). To purify non-degraded PrgA (without the S476A mutation) ...
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bioRxiv - Biochemistry 2020Quote: ... proteins were transferred to a 0.45 μm pore size polyvinylidene fluoride transfer membrane (Merck Millipore), and nonspecific binding was blocked in Tris-buffered saline/0.1% Tween 20 with 5% skim milk powder ...
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bioRxiv - Bioengineering 2021Quote: The proteins were concentrated using Amicon® Ultra Centrifugal filters (Millipore, 100-kDa cut-off) and purified using size exclusion chromatography (SEC ...
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bioRxiv - Biochemistry 2020Quote: Proteins were cross-linked to DNA by the addition of 37% formaldehyde (Sigma-Aldrich; F8775) to a final concentration of 1% for 10 min at the desired time of analysis ...
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bioRxiv - Biophysics 2021Quote: ... the supernatant containing solubilized SLC44A1 protein was load to anti-FLAG M2 affinity gel (Sigma), then washed by Lysis Buffer with 0.02%DDM and 0.004% CHS ...
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bioRxiv - Neuroscience 2021Quote: Luminex multiplexed immunoassays were used to quantify phospho-proteins within the MAPK (Millipore 48-660MAG) and PI3K/Akt/mTOR (Millipore 48-612MAG ...
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bioRxiv - Neuroscience 2021Quote: ... pathways as well as 32 panel of cytokine/chemokine protein expression (Millipore MCYTMAG-70K-PX32) in brain lysates from the Rosa26TurboID/wt AAV and transgenic cohorts ...
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bioRxiv - Neuroscience 2021Quote: ... a quantity of 3.5 μg of protein digest was purified on a Ziptip C18 (Millipore), dried and re-suspended in 100 mM Ammonium formiate (pH 10 ...
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bioRxiv - Neuroscience 2021Quote: ... resolved protein bands were transferred onto Polyvinylidene Fluoride membranes (PVDF, 0.45μm, EMD Millipore, ON, Canada) for 75 minutes on ice in a transfer buffer containing 25 mM Tris ...
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bioRxiv - Molecular Biology 2020Quote: Premature chromosome condensation was induced using the protein phosphatase 1 inhibitor calyculin (Sigma, C5552-10UG). To determine the cell cycle stage of prematurely compacted chromosomes ...
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bioRxiv - Cell Biology 2021Quote: Protein was extracted with RIPA buffer supplemented with protease inhibitor cocktails I and II (Sigma) and phosphatase inhibitor cocktail set III (Calbiochem) ...
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bioRxiv - Cancer Biology 2020Quote: ... total proteins were extracted from cells using ice-cold CHAPS lysis buffer (Sigma-Aldrich, #S7705) and real-time Q-TRAP performed with 0.1 μg protein extracts ...
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bioRxiv - Cell Biology 2021Quote: Proteins pellets from lipid droplet-enriched fractions were resuspended in 0.1 M NaOH (Sigma-Aldrich) and subsequently neutralized using 200 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) ...
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bioRxiv - Cell Biology 2021Quote: ... The protein of interest was purified by over-night incubation with anti-FLAG (Sigma, F1804) or anti-RFA antibody (Agrisera ...
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bioRxiv - Microbiology 2020Quote: ... Immunoreactive protein bands were visualized by the NBT-BCIP method (Sigma-Aldrich™ Argentina S.A). Intensities of bands were quantified from scanned images using ImageJ software and the value obtained from each band was normalized by the loading control SAG1 and then each histone PTM was normalized by their respective histone (H3 or H4).
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bioRxiv - Microbiology 2020Quote: ... the sequence encoding mature proteins without the signal peptide was cloned into pET-14b (+) (Novagen) to generate the expression vector pET14b-6xHis-BcCrh1 ...
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bioRxiv - Systems Biology 2021Quote: ... proteins were transferred onto a PVDF membrane (pore size 0.45 mm, Immobilon-P, Millipore, www.millipore.com) (66 ...
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bioRxiv - Plant Biology 2021Quote: ... Total protein was extracted using an extraction buffer comprised of 50 mM MOPS (M1254, Sigma) pH 7.0 ...
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bioRxiv - Neuroscience 2021Quote: ... the protein bands were visualized through the addition of super enhanced chemiluminescent (ECL) (Millipore, USA) for exposure and imaged by a Gel Image System (Bio-Rad ...
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Q-SNARE Syntaxin 7 confers actin-dependent rapidly replenishing synaptic vesicles upon high activitybioRxiv - Neuroscience 2020Quote: Protein samples were separated on SDS–polyacrylamide mini–gels and blotted onto PVDF membranes (Millipore). As primary antibodies ...
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bioRxiv - Microbiology 2021Quote: Protein samples were resuspended in 200 µL of 100 mM triethylammonium bicarbonate (TEAB) (Sigma-Aldrich), reduced with 20 mM dithiothreitol (DTT ...
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bioRxiv - Immunology 2021Quote: ... 60 pmol of sgRNA was complexed with 10 pmol of Cas9 protein (Sigma-Aldrich, #CAS9PROT) and electroporated into 0.5*105 Jurkat cells per reaction ...
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bioRxiv - Immunology 2021Quote: Cytokine and chemokine protein contents in lung homogenates were quantified using Luminex multianalyte technology (Millipore) according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2020Quote: ... Proteins of the gel were then stained with Coomassie blue R-250 (Sigma-Aldrich, USA) and the molecular mass of the proteins was determined with reference to standard proteins (Thermo Scientific ...
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bioRxiv - Physiology 2021Quote: ... the proteins were transferred to Millipore Immobilon®-P Transfer Membrane (Millipore, Billerica, MA, USA) and blocked in 5% skin milk solution for 2 hours ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitated proteins were eluted two times with 600 μL 250ug/mL 3xFLAG peptides (SIGMA, F4799) in TBS [50mM Tris-HCl pH 7.4 ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were concentrated to ~10-15 mg/ml using 100 kD cutoff centrifugal devices (Millipore), flash-frozen and stored at −80 °C.
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bioRxiv - Systems Biology 2021Quote: ... precipitated SIL-proteins were reconstituted in 104 μL of 8 M urea ([UA]; Sigma-Aldrich) in 0.1 M Trizma® base (pH 8.5 ...