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Citations for Millipore Sigma :
6401 - 6450 of 10000+ citations for QuantiChrom β N Acetylglucosaminidase Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... specimens (n=8 from each group) were sacrificed using a lethal dose (400ppm) of tricaine (MS-222; Sigma) and immediately imaged under light microscopy at 20x before rigor mortis set in to maintain flexibility in the jaw joints ...
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bioRxiv - Neuroscience 2023Quote: All samples were made to pH 6.0 in 50 mM 2-(N-morpholino)ethanesulfonic acid (MES) buffer (Sigma), using potassium as a counterion ...
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bioRxiv - Neuroscience 2023Quote: All samples were made to pH 6.0 in 10 mM 2-(N-morpholino)ethanesulfonic acid (MES) buffer (Sigma), using potassium as a counterion and containing 10% D2O ...
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bioRxiv - Microbiology 2023Quote: Diflufenican (N-(2,4-difluorophenyl)-2-[3-(trifluoromethyl)phenoxy]-3-pyridinecarbox-amide) was purchased from Sigma-Aldrich (Taufkirchen, Germany). Diflufenican metabolites AE B107137 (2-[3-(Trifluoromethyl)phenoxy]nicotinic acid ...
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bioRxiv - Neuroscience 2023Quote: ... Another cohort of C57Bl6 females were treated with PR agonist segesterone (10 mg/kg, sc, n=10, Sigma) or vehicle (20% β-hydroxycyclodextrin ...
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bioRxiv - Cell Biology 2023Quote: ... N=4 (2 females, 2 males) mice were given 12.5 mg/kg sodium nitrite (52mg/L, 23713, Sigma) in 10% blackcurrant squash (Robinsons) ...
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bioRxiv - Microbiology 2022Quote: ... SK-N-BE(2) cell differentiation was induced by 10 μM retinoic acid (RA) treatment (R2625, Sigma-Aldrich) for 5 days ...
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bioRxiv - Cell Biology 2022Quote: ... N-glycosylated proteins were enriched by incubating cell lysates containing protease inhibitor and 1,10-phenanthroline (Sigma-Aldrich, #131377) with 20µl concanavalin A Sepharose beads (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... culture media was replaced by culture media supplemented with 1 mg/mL MTT reagent (Sigma, cat n° M5655). MTT reduction to formazan crystals by cellular oxidoreductases were allowed to form by incubating the cells in a 5% CO2 gas-equilibrated humidified incubator at 37°C for 2 h ...
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bioRxiv - Developmental Biology 2023Quote: ... Animals were killed by rocking gently (50 rpm) in 7.5% N-Acetyl-L-Cysteine (NAc, Sigma A7250-100G) for 15 min at RT ...
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bioRxiv - Bioengineering 2023Quote: ... N-hydroxy-succinimide sodium salt (Sulfo-NHS; 56485) and L-cysteine (30089) were purchased from Sigma-Aldrich (MO). D-cysteine (A110205-011 ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were then reduced in 50 mM Tris HCl (pH 8.2) / 2.4 mM sodium deoxycholate / 2.4 mM sodium N-lauryl sarcosine / 5 mM dithiothreitol (all from Sigma Aldrich). Sulfhydryl groups in cysteines were alkylated with 10 mM 2-chloroacetamide (Fluka ...
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bioRxiv - Biochemistry 2023Quote: ... Lysis buffer was used as a positive control of dead cells with tamoxifen (Sigma-Aldrich Cat. N. T5648).
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bioRxiv - Bioengineering 2024Quote: ... NovaMatrix) was dissolved at 1% w/v in 0.1 M 2-(N-morpholino)ethanesulfonic acid (MES; Sigma-Aldrich), 0.3M NaCl (EMD Millipore ...
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bioRxiv - Cancer Biology 2024Quote: ... and used to transduce N/TERT-1 keratinocytes with a MOI of 100 via spinfection using Polybrene (Sigma) as transfection reagent ...
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bioRxiv - Bioengineering 2024Quote: ... The following chemicals were then added quickly to the 1 wt% alginate solution with the following order: N-hydroxysuccinimide (NHS) (261.6 mg, 2.3 mmol, 0.4 eq., #130672 Sigma Aldrich), EDC (435.5 mg ...
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bioRxiv - Cell Biology 2024Quote: RSL3 (#SML2234) and S-nitroso-N-acetylpenicillamine (SNAP, #N3398) were purchased from Sigma-Aldrich (St. Louis, MO, USA). CPZ (chlorpromazine ...
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bioRxiv - Cell Biology 2024Quote: Lysis was performed in 4 mL of 0.01 % n-dodecyl-beta-maltoside in 60 mM triethyl ammonium bicarbonate (TEAB, pH 8.5, Sigma) at 95 °C in a PCR cycler with a lid temperature of 110 °C for 1 h ...
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bioRxiv - Cancer Biology 2024Quote: ... 3-(2-Methyl-5-nitro-imidazol-1-yl)-N-(2,2,2-trichloro-1-phenylamino-ethyl)-propionamide) (Sigma-Aldrich SML1503). Drugs were diluted in culture media at treatment.
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bioRxiv - Cancer Biology 2021Quote: Cell viability was determined using the 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium assay (MTT assay, Cat. No. M5655, Sigma-Aldrich, St. Louis, MO). Cells were plated in 96-well plates ...
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bioRxiv - Plant Biology 2019Quote: ... the sample was incubated with 700 µL pre-warmed ONPG solution (1mg/mL ONPG (o-Nitrophenyl-β-D-Galactopyranoside, Sigma) prepared in Buffer Z without β-mercaptoethanol at 28°C until a yellow colour developed in the samples without auxin treatment ...
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bioRxiv - Cell Biology 2019Quote: ... coli BL21 cells and the expression of the transgene induced with 1 mM IPTG (isopropyl-β-D-thiogalatopyranoside, Sigma-Aldrich). The recombinant protein was purified with agarose beads that bind specifically to the His-tag (Ni-NTA Agarose ...
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bioRxiv - Neuroscience 2021Quote: ... Animals were subsequently administered 5 μg 17-β-estradiol benzoate in 50uL sesame oil (E2; Sigma-Aldrich, St. Louis, MO) on Day 2 ...
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bioRxiv - Cell Biology 2019Quote: ... Fixed cells were stained with the following antibodies (for 3 hrs at 37°C): β-Tubulin (1:100, Sigma-Aldrich), DBC1 (1:100 ...
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bioRxiv - Molecular Biology 2019Quote: ... 5% glycerol and 2 mM β-mercaptoethanol) supplemented with 1 pill of cOmpleteTM EDTA-free Protease Inhibitor cocktail (Sigma-Aldrich). Cells were then sonicated and the lysate was clarified by centrifugation in a Beckman JA-25.50 rotor at 50000 x g for 1 h at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... plants were incubated overnight at 37°C in GUS buffer (3 mM 5-bromo-4-chloro-3-indolyl-β-D-glucuronide [Duchefa Biochemie, Haarlem, The Netherlands], 0.1% v/v Triton X-100 [Sigma, Steinhaim ...
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bioRxiv - Developmental Biology 2019Quote: ... Antibody and chemical staining were performed in blocking solution at the following dilutions: anti-β-catenin 1:500 (Sigma C2206), anti-laminin 1:500 (Sigma L9393) ...
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bioRxiv - Cell Biology 2019Quote: ... 400 mM KCl, 2 mM MgCl2, 0.002% Brij35, 1 mM β-mercaptoethanol, 1 mM imidazole, 50 mM L-Arginine, Sigma#A5006 ...
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bioRxiv - Biophysics 2019Quote: ... the cells were incubated for 1 hour at room temperature with 1:300 mouse β-tubulin antibody (Sigma Aldrich, T5293) in PBS + 1% BSA ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... or α/β-tubulin (1:1 mixture) (1:100 in PBS; 2 hr at 37°C) (Sigma; T9026, T4026, USA). FITC-conjugated anti-mouse IgG (Jackson ImmunoResearch ...
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bioRxiv - Cancer Biology 2019Quote: ... 1 mg/mL X-Gal (5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside, Sigma Aldrich Aldrich, St. Louis, MO), and 150mM NaCl ...
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bioRxiv - Immunology 2021Quote: ... The animals were then subjected to increasing doses of methacholine (0.1, 0.3, 1, 3, 10, 30mg/mL) (a broncho-constrictor; acetyl-β methacholine chloride; Sigma-Aldrich) delivered by an ultrasonic nebuliser ...
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bioRxiv - Neuroscience 2020Quote: ... The patch pipette was tip filled with internal solution and backfilled with β-escin (~30 - 60 μg·ml−1; E1378; Sigma) or amphotericin B (~200 μg·ml−1 ...
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bioRxiv - Cell Biology 2021Quote: ... 1% β- marcaptoethanol before being washed in 0.5 M EDTA and incubated overnight in 20 mg/mL proteinase K (Sigma), 400 mM EDTA ...
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bioRxiv - Bioengineering 2021Quote: ... Columns were washed with 20 column volumes PBS and recombinant proteins were eluted with 100 mM β-d-lactose (Sigma) prepared in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... OVX females received 5 μg 17-β-estradiol benzoate in 5uL sesame oil (E2, SC; Sigma Aldrich, St. Louis, MO) followed by 10μg E2 in 10μL sesame oil 24 h later ...
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bioRxiv - Neuroscience 2020Quote: ... 6-month-old littermate matched were injected subcutaneously with vehicle [40% (w/v) (2-hydroxypropyl)-β-cyclodextrin (Sigma– Aldrich #332607)] or MLi-2 dissolved in vehicle at a 30 mg/kg final dose ...
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bioRxiv - Biochemistry 2021Quote: ... 1x phosphatase inhibitors (final concentration: 10 mM NaF, 1 mM β-glycerophosphate, 1 mM Na3VO4) and 0.05 unit/μl Benzonase (Sigma). Samples were sonicated in a BioRuptorPico (Diagenode ...
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bioRxiv - Microbiology 2020Quote: Monoclonal antibody against FLAG tag (ANTI-FLAG M2) and β-Actin were purchased from Sigma (Cat.No. F1804 and A2228, respectively). Monoclonal antibody against human IFITM1 (Cat.No ...
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bioRxiv - Cell Biology 2021Quote: MEFs expressing mEmerald-β-actin were plated on the coverslips coated with 10 μg/mL human plasma fibronectin (EMD Millipore). The coverslips with cells were mounted on the microscope slides for imaging ...
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bioRxiv - Cell Biology 2021Quote: The following antibodies were used in this study: FLAG (F1804, 1:2000 for Western blot), β-actin (A1978, 1:10,000 for WB) from Sigma; histone H3 (ab1791 ...
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bioRxiv - Cell Biology 2020Quote: ... the codon optimized gene encoding for rPMDs was subcloned into PET28a (+) and protein expression in E.coli cells was induced with 1 mM isopropyl-β-D-thiogalactoside (IPTG) (Sigma). The his-tagged proteins were purified using Ni-NTA chromatography and the concentration was determined using the BCA estimation kit (Pierce).
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bioRxiv - Microbiology 2022Quote: ... Cells were resuspended in sorbitol buffer (1.2 M sorbitol and 20 mM potassium phosphate buffer, pH 7.2) and spheroplated with β-mercaptoethanol (M7522; Sigma-Aldrich) and zymolase at 30°C ...
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bioRxiv - Bioengineering 2022Quote: ... MSCs were stimulated to undergo osteogenic differentiation with osteogenic medium (osteogenic control medium + 10 mM β- glycerophosphate (G9422, Sigma-Aldrich), 50 μg/ml ascorbic acid-2-phosphate (A8960 ...
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bioRxiv - Plant Biology 2022Quote: ... supplemented with 100 mM β-mercaptoethanol and a 1:50 dilution of protease inhibitor cocktail for fungal extracts (Sigma-Aldrich). Cells were lysed with bead beating using 150 – 200 μL of glass beads (ø 0.5 mm) ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were prepared so that they were at a concentration of 20-30µg and 6X Laemmli sample buffer containing 5% β-mercaptoethanol (Sigma) was added ...
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bioRxiv - Biophysics 2022Quote: ... the proteins were expressed in Escherichia coli strain BL21 Star (DE3) cells using 0.4 mM Isopropyl-β-D-thiogalactopyranoside (IPTG) (Sigma) for 9 to12 hours at 20 °C ...
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bioRxiv - Cell Biology 2022Quote: ... The mouse monoclonal anti-β-actin antibody (catalog #: A1978) and mouse monoclonal FLAG antibody (catalog #: F1804) came from Sigma Aldrich. The fluorescent anti-β-actin antibody Rhodamine came from Biorad (catalog # ...
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bioRxiv - Immunology 2021Quote: ... Lung & spleen cells were diluted to 1×106cells/ml before incubating with 50 μg/ml SARS-CoV-2 purified spike antigen for 24 h in media (RPMI-1640, 10% FCS, 100U/ml penicillin/streptomycin, 5%NEAA, L-glutamine and HEPES, 0.05 mM β-mercaptoethanol (SIGMA)) ...
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bioRxiv - Neuroscience 2020Quote: ... OFC β-endorphin sections were co-labeled with the primary antibody for goat anti parvalbumin (PV+) (1:1000, SIGMA, SAB2500752), a marker for a cortical interneuron subtype ...