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Citations for Millipore Sigma :
601 - 650 of 7146 citations for Recombinant Human Interleukin 6 Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... sera were treated with receptor-destroying enzyme (Sigma-Aldrich, St. Louis, MO), heat inactivated at 56°C ...
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bioRxiv - Neuroscience 2022Quote: ... and anti-glutamate receptor 2 (mouse anti-GluR2 IgG2a; Millipore; 1:2000). Sections were then washed with PBS and incubated in Alexa Fluor-conjugated fluorescent secondary antibodies (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... The blocking buffer for Estrogen receptor (EMD Millipore Cat#04820MI, 1:200) and Ki67 (Abcam Cat#ab16667 ...
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bioRxiv - Neuroscience 2023Quote: ... NMDA receptor antagonist AP-5 (2 mM; Sigma Aldrich, St. Louis, MO), and AMPA receptor antagonist CNQX (0.4 mM ...
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bioRxiv - Neuroscience 2023Quote: ... and AMPA receptor antagonist CNQX (0.4 mM; Sigma Aldrich, St. Louis, MO), or the selective GABA-A receptor agonist muscimol (100 ng ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... nicotinic acetylcholine receptor antagonist mecamylamine (5 mg kg−1,M9020, Sigma-Aldrich) and muscarinic acetylcholine receptor antagonist atropine (5 mg kg−1 ...
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bioRxiv - Neuroscience 2023Quote: ... The D2 receptor (D2R) antagonist haloperidol was also obtained from Sigma-Aldrich, prepared in a 0.9% NaCl solution ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... we tested the broad opioid receptor antagonist (naltrexone hydrochloride (Millipore-Sigma, #N3136)) injected ip at the dosage of 10 ug/uL (aggression N = 10 ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... we tested the broad opioid receptor antagonist (naltrexone hydrochloride (Millipore-Sigma, #N3136)) injected ip at the dosage of 10 ug/uL (aggression N = 10 ...
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bioRxiv - Neuroscience 2023Quote: ... The primary antibodies used included rabbit anti-P2RY12 (Purinergic Receptor P2Y12; Sigma Prestige Antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... Rat Anti-Dopamine D1 Receptor (1:200, Sigma, Cat# D2944, RRID: AB_1840787), Rabbit Anti-Dopamine D2 Receptor (1:250 ...
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bioRxiv - Neuroscience 2024Quote: ... to block NMDA receptors and 100 μM picrotoxin (PTX; P1675 Sigma-Aldrich) to block GABAA receptors ...
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bioRxiv - Neuroscience 2024Quote: ... and 1 µM strychnine hydrochloride (glycine receptor antagonist, Sigma-Aldrich, Cat #: S8753). Drugs were bath applied for at least 5 – 10 minutes before responses were measured.
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bioRxiv - Neuroscience 2020Quote: 6-hydoxydopamine (6-OHDA) solution was freshly prepared by adding 6-OHDA HCl (Sigma) to an appropriate volume of sterile saline (0.9% NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... B cells were cultured in complete RPMI medium supplemented with 10% fetal bovine serum and antibiotics, Interleukin 4 (IL4; made in-house by the Molecular Biology Service, IMP) and 25 μg/ml Lipopolysaccharide (Sigma). All animal experiments were carried out with a valid breeding license (No. ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 0.25x Protease Inhibitor Cocktail for Histidine-tagged proteins (Sigma-Aldrich), and 0.3 mg/mL lysozyme ...
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bioRxiv - Cell Biology 2021Quote: A549 cells expressing GFP-tagged EGFR were obtained from Sigma. Cells were plated on Edge plates (Thermo Scientific ...
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bioRxiv - Genetics 2019Quote: ... The TAP-tagged proteins were probed with PAP (Sigma-Aldrich) (1:2,000 ...
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bioRxiv - Cell Biology 2020Quote: ... FLAG-tagged proteins were immunoprecipitated with αFLAG (clone M2, Sigma) beads (Wang et al ...
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bioRxiv - Biochemistry 2023Quote: ... 10 µl protease inhibitor cocktail for histidine tagged proteins (Sigma) / 0.2 g weight of E ...
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bioRxiv - Plant Biology 2023Quote: ... GST-tagged protein fractions were incubated with thrombin (Sigma, Germany) at 4°C overnight for cleavage of the GST tag (Step 2 ...
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bioRxiv - Bioengineering 2021Quote: ... 6-aminocapuroic acid (6-AA, A2504, Sigma-Aldrich), trans-4-(aminomethyl ...
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bioRxiv - Molecular Biology 2022Quote: ... with 6-Thioguanine (6-TG, Sigma-Aldrich A4660) starting day 7 post-transfection to select for the loss of Hprt and with 1 μM ganciclovir (GCV ...
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bioRxiv - Neuroscience 2022Quote: ... 6-hydroxydopamine (6-OHDA; cat #: 162957; Sigma-Aldrich) was injected similarly into the right striatum ipsilateral to the virus injection ...
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bioRxiv - Plant Biology 2019Quote: 6-Benzylaminopurine (6-BAP) powder from Sigma Aldrich was first dissolved in 10 drops of 1 N NaOH ...
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bioRxiv - Genomics 2019Quote: ... 6-azido-6-deoxy-D-glucose (Sigma 712760) Biotin azide (Thermo Fisher B10184) ...
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bioRxiv - Cell Biology 2019Quote: ... 0.01% 5-(6)-6-carboxyfluorescein diacetate (CFDA; Sigma) and apoplastic tracer ...
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bioRxiv - Genomics 2022Quote: ... 5(6)- carboxyfluorescein (5(6)-FAM) (Sigma Aldrich) (46 ...
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bioRxiv - Neuroscience 2023Quote: 6-OHDA (6-hydroxydopamine hydrobromide, Sigma-Aldrich, 162957) dissolved in sterile saline was injected (1 µL ...
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bioRxiv - Developmental Biology 2021Quote: ... Cumulus-oocyte complexes were collected from the oviduct of wild-type female C57BL/6 mice at 16 h after ovulatory human chorionic gonadotropin (hCG, Sigma-Aldrich) injection and placed in a 50 µL drop of TYH medium that was then covered with mineral oil (Sigma-Aldrich) ...
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bioRxiv - Pathology 2021Quote: PLA was performed in cryostat sections (4–6 μm) obtained from frozen samples of human parietal cortex using the Duolink In Situ Detection Reagents Brightfield system (Sigma-Aldrich). This method monitors the coincidence of specific epitopes and has been previously used for monitoring protein phosphorylation (77) ...
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bioRxiv - Cancer Biology 2020Quote: Biotin labelled and unlabeled RNA oligonucleotides probes corresponding to the human cMYC ARE (1-6) and two random probes (R1, R2) were synthesized as indicated in Table S5 by Sigma-Aldrich. REMSA was performed with a LightShift chemiluminescent RNA EMSA Kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 6 times with 225 µL ELISA wash buffer and 1:5000 goat anti-human IgG HRP (Millipore Sigma) or anti-human IgG isotype specific HRP (Southern Biotech ...
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bioRxiv - Cancer Biology 2022Quote: ... TILs and CAR-TILs were co-cultured with cancer cells for 4-6 hours in RPMI supplemented with human AB serum (Sigma-Aldrich) and CD107a antibody (clone H4A3 ...
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bioRxiv - Cell Biology 2022Quote: ... human very-low-density and low-density lipoproteins (catalog no. 437647-5MG and 6 mg/ml, LP2-2MG, Merck Millipore, respectively) were purchased and stored according to the manufacturers’ instructions ...
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bioRxiv - Neuroscience 2019Quote: ... after which 10 μM 6-TG (6-thioguanine or 2- amino-6-mercaptopurine, Sigma-Aldrich) with 200 μg/ml G418 selection was carried out for an additional 6-8 days ...
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bioRxiv - Biophysics 2020Quote: ... The (His)8-tag was cleaved by thrombin (Sigma) using 20 U per 1 mg GltPh in the presence of 5 mM CaCl2 at room temperature overnight ...
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bioRxiv - Biochemistry 2020Quote: ... and quantitative immunoblotting using an anti-His antibody (Novagen).
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bioRxiv - Cell Biology 2022Quote: ... mouse α-His-HRP (A7058, Sigma-Aldrich, 1:10000).
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bioRxiv - Plant Biology 2019Quote: ... and anti-His (Sigma-Aldrich, St. Louis, MO, USA) antibodies.
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bioRxiv - Biophysics 2022Quote: ... Anti-His mAb was from Sigma (St. Louis, MO). Anti-A1 mAb CR1 was a generous gift from M ...
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bioRxiv - Biochemistry 2023Quote: ... His tag and Flag tag (1:1000; F3165, Sigma).
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bioRxiv - Immunology 2019Quote: ... the stock solution of 6-hydroxydopamine (6-OHDA) (Sigma) was prepared in water at 42 mg/mL and stored at −20°C ...
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bioRxiv - Neuroscience 2023Quote: ... 6-OHDA (10mg/ml; 6-OHDA hydrobromide, Sigma-Aldrich) was dissolved in a vehicle solution immediately before the surgeries ...
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bioRxiv - Immunology 2021Quote: ... were differentiated into macrophages for six days using 50ng/mL granulocyte-macrophage colony-stimulating factor and 25ng/mL interleukin-10 (Sigma, USA), as described previously [24].
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bioRxiv - Immunology 2021Quote: ... SRP4895A), N-(p-Amylcinnamoyl)anthranilic acid (ACA, A8486), PP2 (P0042), interleukin-8 (IL-8, CXCL8 SRP3098) were purchased from Sigma-Aldrich (Sigma-Aldrich Company Ltd. ...
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bioRxiv - Microbiology 2023Quote: ... PBMCs were activated with interleukin-2 (IL-2) 450 UI/mL (Proleukin, Novartis pharma) and polybrene 2 µg/mL (Sigma-Aldrich) during at least 2 h before infection.
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bioRxiv - Neuroscience 2022Quote: ... we used 100 μM picrotoxin (an antagonist of GABAA and GABAC receptors; Sigma). To block glycine receptors ...
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bioRxiv - Neuroscience 2022Quote: ... we used 5 μM SR-95531 (gabazine, an antagonist of GABAA receptors; Sigma). To block both GABAA and GABAC receptors ...
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bioRxiv - Neuroscience 2020Quote: ... The GABAA receptor agonist muscimol (1 mg/mL, Sigma-Aldrich, St. Louis, MO) or vehicle (0.9% sterile saline ...