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Citations for Millipore Sigma :
651 - 700 of 7574 citations for Recombinant Human Interleukin 6 Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... The TAP-tagged proteins were probed with PAP (Sigma-Aldrich) (1:2,000 ...
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bioRxiv - Cell Biology 2020Quote: ... FLAG-tagged proteins were immunoprecipitated with αFLAG (clone M2, Sigma) beads (Wang et al ...
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bioRxiv - Biochemistry 2023Quote: ... 10 µl protease inhibitor cocktail for histidine tagged proteins (Sigma) / 0.2 g weight of E ...
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bioRxiv - Plant Biology 2024Quote: ... GST-tagged protein fractions were incubated with thrombin (Sigma, Germany) at 4°C overnight for cleavage of the GST-tag (Step 2 ...
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bioRxiv - Neuroscience 2020Quote: 6-hydoxydopamine (6-OHDA) solution was freshly prepared by adding 6-OHDA HCl (Sigma) to an appropriate volume of sterile saline (0.9% NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... B cells were cultured in complete RPMI medium supplemented with 10% fetal bovine serum and antibiotics, Interleukin 4 (IL4; made in-house by the Molecular Biology Service, IMP) and 25 μg/ml Lipopolysaccharide (Sigma). All animal experiments were carried out with a valid breeding license (No. ...
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bioRxiv - Bioengineering 2021Quote: ... 6-aminocapuroic acid (6-AA, A2504, Sigma-Aldrich), trans-4-(aminomethyl ...
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bioRxiv - Molecular Biology 2022Quote: ... with 6-Thioguanine (6-TG, Sigma-Aldrich A4660) starting day 7 post-transfection to select for the loss of Hprt and with 1 μM ganciclovir (GCV ...
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bioRxiv - Neuroscience 2022Quote: ... 6-hydroxydopamine (6-OHDA; cat #: 162957; Sigma-Aldrich) was injected similarly into the right striatum ipsilateral to the virus injection ...
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bioRxiv - Plant Biology 2019Quote: 6-Benzylaminopurine (6-BAP) powder from Sigma Aldrich was first dissolved in 10 drops of 1 N NaOH ...
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bioRxiv - Genomics 2019Quote: ... 6-azido-6-deoxy-D-glucose (Sigma 712760) Biotin azide (Thermo Fisher B10184) ...
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bioRxiv - Cell Biology 2019Quote: ... 0.01% 5-(6)-6-carboxyfluorescein diacetate (CFDA; Sigma) and apoplastic tracer ...
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bioRxiv - Genomics 2022Quote: ... 5(6)- carboxyfluorescein (5(6)-FAM) (Sigma Aldrich) (46 ...
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bioRxiv - Neuroscience 2023Quote: 6-OHDA (6-hydroxydopamine hydrobromide, Sigma-Aldrich, 162957) dissolved in sterile saline was injected (1 µL ...
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bioRxiv - Developmental Biology 2021Quote: ... Cumulus-oocyte complexes were collected from the oviduct of wild-type female C57BL/6 mice at 16 h after ovulatory human chorionic gonadotropin (hCG, Sigma-Aldrich) injection and placed in a 50 µL drop of TYH medium that was then covered with mineral oil (Sigma-Aldrich) ...
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bioRxiv - Pathology 2021Quote: PLA was performed in cryostat sections (4–6 μm) obtained from frozen samples of human parietal cortex using the Duolink In Situ Detection Reagents Brightfield system (Sigma-Aldrich). This method monitors the coincidence of specific epitopes and has been previously used for monitoring protein phosphorylation (77) ...
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bioRxiv - Cancer Biology 2020Quote: Biotin labelled and unlabeled RNA oligonucleotides probes corresponding to the human cMYC ARE (1-6) and two random probes (R1, R2) were synthesized as indicated in Table S5 by Sigma-Aldrich. REMSA was performed with a LightShift chemiluminescent RNA EMSA Kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 6 times with 225 µL ELISA wash buffer and 1:5000 goat anti-human IgG HRP (Millipore Sigma) or anti-human IgG isotype specific HRP (Southern Biotech ...
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bioRxiv - Cancer Biology 2022Quote: ... TILs and CAR-TILs were co-cultured with cancer cells for 4-6 hours in RPMI supplemented with human AB serum (Sigma-Aldrich) and CD107a antibody (clone H4A3 ...
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bioRxiv - Cell Biology 2022Quote: ... human very-low-density and low-density lipoproteins (catalog no. 437647-5MG and 6 mg/ml, LP2-2MG, Merck Millipore, respectively) were purchased and stored according to the manufacturers’ instructions ...
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bioRxiv - Neuroscience 2019Quote: ... after which 10 μM 6-TG (6-thioguanine or 2- amino-6-mercaptopurine, Sigma-Aldrich) with 200 μg/ml G418 selection was carried out for an additional 6-8 days ...
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bioRxiv - Biophysics 2020Quote: ... The (His)8-tag was cleaved by thrombin (Sigma) using 20 U per 1 mg GltPh in the presence of 5 mM CaCl2 at room temperature overnight ...
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bioRxiv - Biochemistry 2020Quote: ... and quantitative immunoblotting using an anti-His antibody (Novagen).
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bioRxiv - Cell Biology 2022Quote: ... mouse α-His-HRP (A7058, Sigma-Aldrich, 1:10000).
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bioRxiv - Plant Biology 2019Quote: ... and anti-His (Sigma-Aldrich, St. Louis, MO, USA) antibodies.
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bioRxiv - Biophysics 2022Quote: ... Anti-His mAb was from Sigma (St. Louis, MO). Anti-A1 mAb CR1 was a generous gift from M ...
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bioRxiv - Biochemistry 2023Quote: ... His tag and Flag tag (1:1000; F3165, Sigma).
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bioRxiv - Immunology 2019Quote: ... the stock solution of 6-hydroxydopamine (6-OHDA) (Sigma) was prepared in water at 42 mg/mL and stored at −20°C ...
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bioRxiv - Neuroscience 2023Quote: ... 6-OHDA (10mg/ml; 6-OHDA hydrobromide, Sigma-Aldrich) was dissolved in a vehicle solution immediately before the surgeries ...
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bioRxiv - Immunology 2021Quote: ... were differentiated into macrophages for six days using 50ng/mL granulocyte-macrophage colony-stimulating factor and 25ng/mL interleukin-10 (Sigma, USA), as described previously [24].
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bioRxiv - Immunology 2021Quote: ... SRP4895A), N-(p-Amylcinnamoyl)anthranilic acid (ACA, A8486), PP2 (P0042), interleukin-8 (IL-8, CXCL8 SRP3098) were purchased from Sigma-Aldrich (Sigma-Aldrich Company Ltd. ...
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bioRxiv - Microbiology 2023Quote: ... PBMCs were activated with interleukin-2 (IL-2) 450 UI/mL (Proleukin, Novartis pharma) and polybrene 2 µg/mL (Sigma-Aldrich) during at least 2 h before infection.
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bioRxiv - Neuroscience 2022Quote: ... we used 100 μM picrotoxin (an antagonist of GABAA and GABAC receptors; Sigma). To block glycine receptors ...
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bioRxiv - Neuroscience 2022Quote: ... we used 5 μM SR-95531 (gabazine, an antagonist of GABAA receptors; Sigma). To block both GABAA and GABAC receptors ...
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bioRxiv - Neuroscience 2020Quote: ... The GABAA receptor agonist muscimol (1 mg/mL, Sigma-Aldrich, St. Louis, MO) or vehicle (0.9% sterile saline ...
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bioRxiv - Neuroscience 2021Quote: ... and the N-methyl-D-aspartate receptor agonist D-Glutamate (0.75mM, Sigma-Aldrich) was bath applied to the spinal cord.
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bioRxiv - Neuroscience 2020Quote: ... and (3) GluA2 (mouse anti-glutamate receptor 2; Millipore; used at 1:2000). Cochlear pieces were incubated in species appropriate secondary antibodies coupled to Alexa Fluors in the red ...
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bioRxiv - Neuroscience 2020Quote: ... Extracellular solution containing the opioid receptor antagonist naloxone (300 µM, Sigma Aldrich, Germany) was perfused to the MOB slice for at least 10 minutes.
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bioRxiv - Neuroscience 2020Quote: ... the D2 DA receptor (D2R) agonist quinpirole (20 μM, Sigma, St. Louis, MO) was applied at the end of each experiment as a pharmacological test of DA neuron ientity.25 Whole-cell somatodendritic nAChR-mediated currents were evoked in the presence of tetrodotoxin (TTX ...
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bioRxiv - Neuroscience 2020Quote: ... mouse (IgG2a) anti-GluA2 (Glutamate receptor subunit A2) at 1:2000 (#MAB397, Millipore) for labeling post-synaptic receptor patches ...
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bioRxiv - Neuroscience 2020Quote: ... and the β-adrenergic receptor antagonist DL-propranolol (5 mg/kg; Sigma-Aldrich), immediately prior to sleep latency testing ...
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bioRxiv - Neuroscience 2019Quote: ... and GABA receptors was measured by application of 100 μM glutamic acid (Sigma) or 3 μM GABA (Sigma) ...
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bioRxiv - Neuroscience 2021Quote: ... and glutamate receptors (NBQX, 10 μM, Tocris; and DL-AP5, 30 μM, Sigma).
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bioRxiv - Cell Biology 2022Quote: ... a mouse anti-GABAA receptor β2/β3 subunit antibody (Millipore, catalog #: 05-474), or a mouse anti-His antibody.
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bioRxiv - Neuroscience 2022Quote: ... the non-competitive NMDA receptor antagonist MK801 (Sigma-Aldrich, St. Louis, MO, USA) was administered in a separate cohort (0.3 mg/kg ...
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bioRxiv - Neuroscience 2022Quote: ... we used a glutamatergic receptor antagonist (10 μM cyanquixaline, or CNQX, Millipore-Sigma) and a GABAA chloride channel blocker (10 mM picrotoxin ...
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bioRxiv - Neuroscience 2022Quote: ... we used a glutamatergic receptor antagonist (10 μM cyanquixaline, or CNQX, Millipore-Sigma) and a GABAA chloride channel blocker (10 mM picrotoxin ...
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bioRxiv - Neuroscience 2024Quote: ... the GABA-A receptor antagonist gabazine (SR-95531, Sigma Aldrich,St. Louis, MO) was applied bilaterally (0.005mM-0.01mM in PBS (Carl Roth ...
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bioRxiv - Cell Biology 2024Quote: ... the EP2 receptor antagonist AH6809 (10μM in DMSO, day 0-42; Sigma-Aldrich) and the EP4 receptor antagonist AH23848 (10μM in DMSO ...
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bioRxiv - Physiology 2022Quote: Drugs used throughout studies include MSX-3 (A2A receptor antagonist; #M3568; Millipore Sigma), ketanserin tartrate (5-HT2A/C receptor antagonist ...