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Citations for Millipore Sigma :
6051 - 6100 of 10000+ citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Universal matrix composition: mixture of 1:1 of α-cyano-4-hydroxycinnamic acid (CHCA) and 2,5-dihydroxybenzoic acid (2,5-DHB) (Sigma-Aldrich solubilized in 78:22 MeCN in H2O + 0.1% TFA.
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bioRxiv - Neuroscience 2020Quote: ... for 2 hours and stained with primary antibodies overnight at 4°C (guinea pig anti-vGlut2, 1:1000, Millipore AB2251-I; guinea pig anti-vGlut1, 1:1000, Millipore AB5905 ...
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bioRxiv - Molecular Biology 2020Quote: ... and expression of the protein was induced for 4 hours with 1 mM isopropyl 1-thio-B-D-galactopyranoside (Sigma) at 18°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... Cells were cultured in 4 mL CgXII medium containing 13C-1-glucose or 13C-1-fructose (Sigma-Aldrich, Taufkirchen, Germany) as sole carbon sources ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 48 h at 4 βC with primary antibodies (rat anti-BrdU, 1:500, OBT Serotec; mouse anti-NeuN, 1:200, MAB377, Millipore; rabbit anti-S100β ...
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bioRxiv - Microbiology 2021Quote: ... tissues were incubated until dissolved at 37°C in 495 μL extraction buffer [(4 M urea, 0.2 M NaCl, 1 mM trisodium citrate, 1% SDS (Sigma-Aldrich)] supplemented with 5 μL Proteinase K (20 mg mL−1 ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated with the primary antibodies of interested proteins at 4°C overnight (Nestin, 1:1000, Sigma; Tuj1, 1:1000, Sigma; GFAP ...
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bioRxiv - Microbiology 2021Quote: ... Phage 80α stock was diluted in phage buffer (50 mM Tris-HCl pH 7.8, 1 mM MgSO4, 4 mM CaCl2 and 1 g/L gelatin; Sigma–Aldrich), and added to the master mix to reach the desired starting concentration in plaque forming units per mL (pfu/mL) ...
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bioRxiv - Physiology 2023Quote: ... sections were exposed overnight at 4°C to antibodies detecting CARNS1 (rabbit polyclonal, 1:100 in PBS with 1% BSA, HPA038569, Sigma). The next day ...
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bioRxiv - Cell Biology 2023Quote: ... the reaction between peptide conjugate and crosslinkers (PEG-4VS and PEG-4 ACLT) was performed in stoichiometric ratio 1:1 in30mM HEPES buffer at pH 8 (Sigma) diluted in 1x HBSS (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... Th17 cells were split (usually 1:3) and transduced by adding 1:80-diluted retroviral supernatant and 4 µg/ml polybrene (Sigma) in addition to 10 ng/ml IL-23 and 10 µg/ml anti-IFNγ antibody (all diluted in pre-warmed T cell medium and calculated for culture volume) ...
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bioRxiv - Immunology 2023Quote: Mouse lungs were diced into small pieces using razor blades and incubated for 1 h in digestion medium (RPMI-1640 with 1 mg/mL collagenase type 4, Sigma) at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... MO, USA], yeast extract 4 g.L-1 [Sigma-Aldrich, St. Louis, MO, USA], malt extract 10 g.L-1 [Sigma-Aldrich, St ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed 4 times with PBS and resuspended in PBS containing 1% BSA and DAPI (1 μg/mL; Sigma) as a nuclear stain ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated with the primary antibodies at 4 °C for 48 h (GFP, chicken, abcam, 1:2000; GFAP, rat, Invitrogen, 1:1000; NeuN, guinea pig, Millipore, 1:2000 ...
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bioRxiv - Immunology 2022Quote: ... primary antibodies were incubated overnight at 4°C: mouse monoclonal anti-C5aR1 (clone: S5/1; Millipore; cat. MABF1980; 1:50), rabbit polyclonal anti-IBA1 (FUJIFILM Wako Pure Chemical Corporation ...
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bioRxiv - Developmental Biology 2024Quote: ... washed with distilled water and fixed on a shaker for 20 minutes in a 1:1 mix of 4% Paraformaldehyde (Proscitech) in 1xPBS and Heptane (Sigma). Embryos were then washed 3 times with absolute methanol and 3 times with PBS/T and blocked for 1 hour in 0.1% PBS/T containing 10% normal goat serum (NGS) ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated overnight at 4 °C with rabbit anti-mouse Aβ(1–40/42) antiserum (1:200, Millipore) or polyclonal rabbit anti-mouse ApoB (1:200 ...
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bioRxiv - Cell Biology 2020Quote: ... tissue engineered cartilage was digested with papain solution (25 μg/mL papain, 2 mM cysteine, 50 mM sodium phosphate, and 2 mM EDTA adjusted to pH 6.5 [all from Sigma-Aldrich]) at 65 °C for 3h ...
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bioRxiv - Cell Biology 2020Quote: ... 150 mM NaCl, 2 mM EGTA, 2 mM MgCl2, 0.5% NP-40, 1mM DTT, 1U/ml Benzonase from Novagen/EMD Millipore, Temecula ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 2-Heptyl acetate was synthesized by mixing acetic acid with an excess of 2-heptanol and H2SO4 (all Sigma-Aldrich) as catalyst and subsequently heating the mixture for 48 h at 45 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... Cysteine thiols were subsequently reduced and alkylated by adding Tris(2-carboxyethyl)phospin (Carl Roth) to a final concentration of 10 mM and 2-Chloroacetamide (≥ 98.0 %, Sigma-Aldrich) to a final concentration of 40 mM ...
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bioRxiv - Evolutionary Biology 2022Quote: ... A volume of 40 μL (corresponding to 4.106 cells/cm2) of suspension was plated on 6 cm plates filled with 2 mL of 2% Phytagel (Sigma-Aldrich) as previously described by Dubravcic et al ...
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bioRxiv - Neuroscience 2021Quote: ... For immunofluorescence stainings slices were incubated for 1 h at room temperature (RT) in blocking buffer (2% gelatine, 2% BSA (bovine serum albumin, Cat#a2153, CAS Number: 9048-46-8, Sigma), 0.1% Triton X-100 (Merck ...
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bioRxiv - Genetics 2021Quote: Worms were prepared at indicated ages and transferred on a 2% agarose pad with 100 mM sodium azide (DAEJUNG, Siheung, South Korea) or 2 mM levamisole (tetramisole; Sigma). The images of worms were captured by using an AxioCam HRc CCD digital camera (Zeiss Corporation ...
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bioRxiv - Neuroscience 2020Quote: ... we topically applied over the craniotomy 2 μL of PBS containing 25 μM of 2-guanidinoethylmercaptosuccinic acid (GEMSA; Sigma-Aldrich) without removing the electrode ...
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bioRxiv - Biochemistry 2021Quote: ... Cysteine Biotinylation was carried out using Poly(ethylene glycol) [N-(2-maleimidoethyl)carbamoyl]methyl ether 2-(biotinylamino)ethane (Sigma 757748) (Biotin-maleamide) ...
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bioRxiv - Cell Biology 2021Quote: ... were reared in 10g/l of glucose/200µM of 3BDO (3-Benzyl-5-((2-nitrophenoxy) methyl)-dihydrofuran-2(3H)-one) (Sigma Aldrich) supplemented in standard fly food ...
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bioRxiv - Neuroscience 2020Quote: ... Tubes were placed in a cylindrical phantom (2 cm diameter, 2 % agarose mixed with 5 % intralipid (Sigma-Aldrich AG, Switzerland). The phantom holder was placed in an imaging chamber filled with water and kept at 28°C.
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bioRxiv - Bioengineering 2020Quote: ... A 2 mm by 2 mm piece of Kim wipe was soaked in 30% Fluoro-Ruby (FR; EMD Millipore, AG335) and placed inside the silicon nerve cuff toward the bottom ...
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bioRxiv - Physiology 2020Quote: ... Protocol 2 (Pilocarpine): Six participants had 2 intradermal microdialysis membranes inserted and perfused with 1.67 mg/ml pilocarpine (Sigma-Aldrich) prior to the same B2R antagonist and agonist approach described in Protocol 1 (Figure 1B) ...
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bioRxiv - Biochemistry 2020Quote: 2-amino-2-hydroxymethyl propane-1,3-diol (Tris BioUltra for molecular biology) was purchased from Sigma-Aldrich (cat. no. 93362). RNA oligonucleotides were purchased from Biomers.net (Ulm ...
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bioRxiv - Microbiology 2020Quote: 2 μg mRNA suspended in 6 μl RNAse-free H2O was digested with 2 units of nuclease P1 (Sigma N8630) for 3 h at 37 ºC ...
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bioRxiv - Microbiology 2020Quote: ... Benztropine mesylate (CAS No. 132-17-2) and Bafilomycin A1 (CAS No. 88899-55-2) were purchased from Sigma-Aldrich. Active pharmaceutical ingredients for an additional 59 drugs ...
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bioRxiv - Biochemistry 2020Quote: ... Day 1 adult animals were mounted on 2% (vol/vol) agar pads and immobilized with 30 mg/mL 2-3-butaneione monoxime (BDM, Sigma) in M9 buffer.
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bioRxiv - Biochemistry 2020Quote: ... animals were mounted on 2% (vol/vol) agar pads and immobilized in 30 mg/mL 2-3-butaneione monoxime (BDM, Sigma) in M9 buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... They were then differentiated into cardiac mesoderm using RPMI B27-medium (RPMI1640 GlutaMAX+ 2% B27 supplement without insulin, 200 µM L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate [Sigma] and 1% Penicillin/Streptomycin [all ThermoFisher Scientific unless otherwise indicated] ...
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bioRxiv - Cell Biology 2021Quote: ... and re-suspended in equal volume of 2×Native lysis buffer (200mM Tris pH 7.5, 150mM NaCl, 2mM PMSF, 2×Protease Inhibitor Cocktails (Sigma-Aldrich)) ...
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bioRxiv - Microbiology 2021Quote: ... of 2 in the presence of 2.2 mM ferrihydrite (Schwertmann and Fischer, 1973; Stookey, 1970) and 2 mM ferrozine (Sigma-Aldrich). Where indicated ...
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bioRxiv - Neuroscience 2021Quote: ... NEL-MG were treated with 2 μL of red fluorescent latex beads (2 μm, Sigma-Aldrich, St. Louis, MO, USA), HiLyte™ Fluor 488-labeled amyloid β peptide 25-35 (2 μL) ...
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bioRxiv - Cell Biology 2021Quote: ... hPSCs were cultured in RPMI B27-medium (RPMI 1640 GlutaMAX+ 2% B27 supplement without insulin, 200 μM L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate (Sigma) and 1% Penicillin/Streptomycin (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Mesoderm was induced with RPMI B27- (RPMI 1640 GlutaMAX+ 2% B27 supplement without insulin, 200 μM L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate (Sigma) and 1% Penicillin/Streptomycin (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... the dyes were removed by flowing 2.5 mL of cleavage buffer comprising 2× SSC and 50 mM of Tris (2-carboxyethyl) phosphine (TCEP; Sigma, 646547) with 15 min incubation in the flow chamber to cleave the dyes linked to the readout probes through disulfide bond ...
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bioRxiv - Neuroscience 2023Quote: The mice received three injections every 2 h (200 ul/injection, intraperitoneally in alternative sites of the body) of 5-Chloro-2’-deoxy-Uridine (CldU, Sigma reference C6891 ...
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bioRxiv - Microbiology 2023Quote: ... the reaction was stopped by FBS then cell were washed 2 time with PBS-based staining buffer containing 2% fetal bovine serum (FBS) and 0.01% NaN3 (Sigma-Aldrich), blocked Fc IgG receptors by pre-incubated with CD16/32(93 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were grown overnight in SC-URA medium with 2% dextrose and were plated in SC-URA plates in the presence of 2% dextrose (Sigma) or 2% galactose (Difco) ...
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bioRxiv - Neuroscience 2022Quote: ... mice were injected with the noradrenergic neuron specific neurotoxin N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine hydrochloride (DSP4; Sigma, C8417) once at a dose of 50 mg/kg (i.p.) ...
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bioRxiv - Molecular Biology 2024Quote: ... and human epithelial type 2 (HEp-2, CCL-23, ATCC) cells were cultured in Dulbecco’s modified eagle medium (DMEM, Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Genetics 2024Quote: ... acr-2(gf) double mutants were washed into a 2 mL solution of M9 media with 47 mM ethyl methanesulfonate (Sigma) and incubated at 22°C on a spinning wheel for 4 hours ...
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bioRxiv - Immunology 2023Quote: Duodenal biopsies were collected in ice-cold RPMI-1640 and processed into single- cell suspensions by treatment with 2 mM EDTA in 2% (v/v) FBS/PBS followed by digestion with collagenase (Sigma) and cryopreservation as previously described.22 PBMCs were isolated from peripheral blood by density gradient centrifugation followed by cryopreservation ...