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Citations for Millipore Sigma :
5951 - 6000 of 10000+ citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Adherent iRBCs were counted using 40x lens with a Nikon ECLIPSE TE200 in 10 randomly selected fields (each with 0.2 μm2) before 2 our fixation with 2% glutaraldehyde (G5882; Sigma)/PBS ...
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bioRxiv - Immunology 2023Quote: ... and the cells overlaid with DMEM containing 1.6% agarose solution and 2 μg/ml L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK)-treated trypsin (Sigma). The plates were incubated at 35°C for 48 hours ...
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bioRxiv - Immunology 2023Quote: Tumor cells were washed in PBS and pellets were lysed for 20 minutes on ice in lysis buffer (100mM Hepes pH 7.5, 300mM NaCl, 10mM EGTA, 3mM MgCl2, 2% glycerol, 2% triton X-100 (Sigma), protease inhibitor cocktail (Roche 5892791001 ...
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bioRxiv - Physiology 2024Quote: Mice subjected to SNT were anesthetized through intraperitoneal injection of 0.5 mg/g avertin (2,2,2,-Tribromoethanol 97%, Sigma #T48402, and 2-methyl-2-butanol 99%, Sigma #240486-100ml). Soleus muscles were freshly dissected and embedded into O.C.T compound (23-730-571 ...
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bioRxiv - Microbiology 2023Quote: ... 2% sodium dodecyl sulfate [SDS]) supplemented with 2 mM MgCl2 and 0.1 units/μL Benzonase (Sigma catalog number E1014), incubated for 5 min at 37°C ...
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bioRxiv - Immunology 2023Quote: ... then the plates were blocked for 2 hours at room temperature with 2% bovine serum albumin (Sigma-Aldrich A2153) prior to use ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated with 50 µL of a 2 mg/mL solution of (3-(4,5-dimethyl-thiazole-2-yl)-2,5-biphenyl tetrazolium (MTT, Sigma-Aldrich) in PBS for 4 h and then exposed to 100 µL dimethyl sulfoxide (DMSO ...
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bioRxiv - Plant Biology 2023Quote: ... Nicotiana tabacum Bright Yellow 2 (BY-2) cell suspension cultures were grown in MS basal salt mixture (M5524; Sigma) supplemented with 3% (w/v ...
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bioRxiv - Immunology 2023Quote: ... in culture medium (RPMI 1640 + 10% FBS, 10% penicillin/streptomycin, 50mM 2-ME (Sigma-Aldrich, Cat# 60-24-2), and 1000x BD Golgi Plug containing Brefeldin A (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... BAL cells were obtained by washing the lung airway with PBS containing 2% FBS and 2 mM EDTA (Sigma). Lungs were processed as previously described52 ...
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bioRxiv - Developmental Biology 2024Quote: Adult zebrafish were injected intraperitoneally at 2 dpci with 20 μl of 2.5 mg/ml of 5-Bromo-2-deoxyuridine (BrdU, B5002-1G, Sigma). Livers were collected and processed for analysis at 5 dpci ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were treated with either fresh EGM-2 or EGM-2 containing 100 μM of sodium arsenite (Sigma-Aldrich) for 1 hour prior to fixation and staining ...
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bioRxiv - Microbiology 2024Quote: ... equimolar amounts of antibodies containing either the F405L or K409K mutation were mixed with a solution of 2-mercaptoethylamine (2-MEA, 750 mM, pH 7.4; Sigma) and PBS and incubated for 5h at 31°C ...
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bioRxiv - Biochemistry 2024Quote: ... 2.5 μl of purified protein (0.5 mg/ml) was applied to Quantifoil Cu 300 mesh R 2/2 grids coated with graphene oxide (Sigma) (52) ...
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bioRxiv - Cell Biology 2024Quote: ... 2 × 107 apoptotic cells were incubated with 3 U of DNase I or 2 U of proteinase (Sigma-Aldrich) in PBS at 37°C for 30 min ...
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bioRxiv - Bioengineering 2024Quote: ... the singularized cells were resuspended in 2% PBS+FBS buffer solution (2% fetal bovine serum (Sigma-Aldrich; F2442-500ML) in phosphate-buffered saline (PBS ...
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bioRxiv - Genomics 2020Quote: Human melanoma cells were seeded in 12 well plates with increasing volumes of lentiviral supernatant in a final volume of 2 mL with 4 μg/mL polybrene (Millipore, Burlington, MA). Plates were centrifuged for 2 hours at 1,000x g at 30°C and cells were incubated for 16 hours at 37°C and 5% CO2 in a humidified incubator ...
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bioRxiv - Genomics 2020Quote: Human melanoma cells were seeded in 12 well plates with increasing volumes of lentiviral supernatant in a final volume of 2 mL with 4 μg/mL polybrene (Millipore, Burlington, MA). Plates were centrifuged for 2 hours at 1,000x g at 30°C and cells were incubated for 16 hours at 37°C and 5% CO2 in a humidified incubator ...
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bioRxiv - Microbiology 2020Quote: ... cultured for 24 h at 37°C with 5% CO2 and transduced with pseudoviruses harboring VSV-G or S-SARS-CoV-2 proteins or lacking the fusion protein (∆Env) in the presence of polybrene (4 µg/ml; Sigma-Aldrich, Poland) and HTCC-77 (100 μg/ml ...
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bioRxiv - Systems Biology 2022Quote: ... the pellicles were first digested by shaking for 16 h at 4 °C in 15 ml of PBS buffer with 2% cellulase (Sigma Aldrich, C2730).
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bioRxiv - Neuroscience 2021Quote: ... The supernatant became the soluble fraction and the pellet was solubilized in urea buffer (30 mM Tris, 7 M Urea, 2 M Thiourea, 4% CHAPS, 1X Protease Inhibitor Cocktail (Millipore Sigma 11873580001), 0.5 mM PMSF ...
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bioRxiv - Microbiology 2020Quote: Zn2+release from DksA was measured using the metal chelator 4-(2-pyridylazo)resorcinol (PAR) (Sigma-Aldrich, St. Louis, MO, United States) (59) ...
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bioRxiv - Biochemistry 2020Quote: ... Unbound dye was removed by 2 washes with 100X excess of protein storage buffer followed by centrifugation in Amicon® Ultra-4 Centrifugal Filter Units (SIGMA MilliPORE). LLPS of dyed and undyed protein was compared as quality control and results were similar (data not shown).
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bioRxiv - Molecular Biology 2020Quote: ... Table 2) and were introduced by 4 lentiviral infections at 12hr intervals in presence of 8 μg/ml polybrene (Sigma-Aldrich®) using supernatant from transfected 293T cells ...
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bioRxiv - Immunology 2022Quote: ... the retrovirus supernatant was concentrated by centrifugation at 5000g at 4 °C for 2 h with Amicon Ultra-15 Centrifugal Filter Units (Millipore, Billerica, MA). A human T cell line (Jurkat ...
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bioRxiv - Neuroscience 2021Quote: ... For immunostaining cells were fixed with 4% PFA in cytoskeleton preservation buffer (10mM 2-(N-morpholino) ethanesulfonic acid (MES, Sigma-Aldrich, M3671) pH 6.1 ...
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bioRxiv - Neuroscience 2020Quote: ... After rinsing three times for 10 minutes in TBS at RT sections were incubated with 4?,6-Diamidin-2-phenylindol (Dapi, Sigma, Cat# D9542) for 10 minutes at RT and washed once for 10 minutes in TBS ...
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bioRxiv - Biophysics 2023Quote: ... with a voxel-size of 0.636 × 0.636 × 2 μm) are recorded once before and 4 h after actin-depolymerization using 10 μM cytochalasin D (Sigma-Aldrich, St. Louis) using brightfield and second-harmonic generation imaging microscopy ...
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bioRxiv - Physiology 2023Quote: ... glycerol:relaxing solution (in mM: 100 KCl, 10 imidazole, 2 EGTA, 5 MgCl2, and 4 ATP) containing 1X protease inhibitor cocktail (Sigma-Aldrich P8340) and 1% triton X-100 ...
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bioRxiv - Genomics 2023Quote: ... 160 μl of 2x lysis buffer (2 % Triton x-100, 100 mM Tris-HCl pH 8.0, 300 mM NaCl, 0.2% sodium deoxycholate (Sigma-Aldrich, 302-95-4), 10 mM sodium butyrate (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... were coated overnight with retronectin solution (32 µg/mL in PBS, WEHI) at 4℃ followed by blocking with 2% bovine serum albumin solution (Sigma-Aldrich, #A1595) in PBS at 37℃ for 30 min prior to coating with viral supernatant ...
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bioRxiv - Molecular Biology 2024Quote: ... The number of viable cells was determined by mitochondrial conversion of 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma Aldrich, Missouri, USA) to formazan ...
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bioRxiv - Neuroscience 2023Quote: ... Animals were induced with 2-4% isoflurane and injected with 15 µL of CFA (Sigma-Aldrich F5881. Sigma, St. Louis, MO, USA) subcutaneously in the snout ...
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bioRxiv - Neuroscience 2023Quote: ... Animals were induced with 2-4% isoflurane and injected with 15 µL of CFA (Sigma-Aldrich F5881. Sigma, St. Louis, MO, USA) subcutaneously in the snout ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells exposed to glutamine starvation were also treated with a freshly made 2 mM methionine sulfoximine (MSX, Sigma Aldrich 15985-39-4). The TCA precipitated samples were then centrifuged at 4000 rpm for 5 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Chromatin from about 1.5×106 cells was incubated overnight at 4°C with 2–5 μg antibodies to FLAG epitope (M2; Sigma-Aldrich, #F1804, RRID:AB_262044), BRD4 (Cell Signaling Technology ...
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bioRxiv - Immunology 2024Quote: ... counted and 2-4×106 cells were washed twice in ice cold 0.9% saline made with NaCl (Sigma Aldrich, 31434-500G-M) and ultrapure HPLC grade water (Alfa Aesar ...
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bioRxiv - Cell Biology 2022Quote: Cultured cells were fixed with 4% paraformaldehyde (PFA) and 2% sucrose and then stained with Nile red (Sigma-Aldrich, St. Louis, MO) and HCS Cell Mask Blue (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... EGTA-eluates were pooled and concentrated to a final concentration of ∼2 mg/ml using an Amicon Ultra-4 cellulose centrifugal filter unit (Merck Millipore, Cat# UFC810024).
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bioRxiv - Biochemistry 2022Quote: ... sodium hydroxide (1310-73-2) and α-amylase (DFP Treated, Type I-A, saline suspension, 647-015-00-4) were purchased from Sigma-Aldrich Company Ltd. ...
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bioRxiv - Biochemistry 2022Quote: ... An amount of 2-4 mg of whole cell extract as incubated with 25 μL ANTI-FLAG® M2 affinity gel (Millipore Sigma) overnight at 4°C ...
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bioRxiv - Genomics 2022Quote: ... Samples were cryoprotected in a 30% sucrose solution (w/v) overnight at 4°C and frozen in 2-methylbutane (Sigma-Aldrich 106056) on dry ice ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated 2 hr at 4°C with rotation after addition of either 6 μg mouse αSFPQ (Sigma-Aldrich WH0006421M2, RRID:AB_1843565)) or 6 μg normal mouse αIgG (Santa Cruz sc-2025 ...
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bioRxiv - Neuroscience 2024Quote: ... The β- hexosaminidase assay was performed in a 96-well plate by mixing 50 μL of 2 mg/mL 4-nitrophenyl N-acetyl-β-D-galactosaminide (N9003, Millipore Sigma) in 0.1 M citrate buffer (pH 4.5 ...
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bioRxiv - Plant Biology 2024Quote: ... WTN1 Δ 23-HA and PWT4-HA proteins were incubated at 4°C for 2 h to capture Flag-tagged proteins using Flag-Trap magnetic beads (Sigma-Aldrich, M8823). Following incubation ...
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bioRxiv - Neuroscience 2021Quote: ... the mice were given an intraperitoneal injection of 10 mg kg-1 of 4-hydroxytamoxifen (4-OHT; Sigma, H6278) dissolved in a 1:4 mixture of castor oil (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... tadpoles were euthanized using MS-222 (1 g/L) and fixed in 4% paraformaldehyde (Sigma - CAS 30525-89-4) for 3 h at room temperature (RT) ...
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bioRxiv - Genetics 2023Quote: ... embryos were fixed in 4% paraformaldehyde overnight at 4°C, washed in PBST (1× PBS, 0.1% Tween 20 (Sigma)) ...
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bioRxiv - Neuroscience 2023Quote: ... and the sample was incubated for 1.5h rotating at 4°C before the addition of 1/4 volume of 3.06% cycloamylose (Sigma-Aldrich). The sample was incubated overnight rotating at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Penicillin-Streptomycin (Thermo-Fisher, #15070063, 100X stock solution) and 10 nM 20-hydroxy-ecdysone (Sigma, H5142) to the medium ...