Labshake search
Citations for Millipore Sigma :
5851 - 5900 of 10000+ citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2022Quote: The HEK-pAGDisplay-RBD stable cells grown to 80% confluency were gently detached by Accutase (1/2 solution in PBS, 3 min, Sigma Aldrich cat. A6964) and washed by PBS with 2 g/L BSA (PBSB) ...
-
bioRxiv - Neuroscience 2020Quote: ... Adult animals (age 8-12 months) of either sex were dark-adapted for 2 h and euthanized by immersion in 1 mM tricaine methanesulfonate (SIGMA, cat. no. A5040) in pH 7.0 buffered system water prior to experiments.
-
bioRxiv - Evolutionary Biology 2022Quote: ... The pellet was resuspended in 1 mL PBT (1X PBS, 0.1% Triton X-100, 2% BSA, 0.2U/µL protease inhibitor (Sigma Aldrich cOmplete protease inhibitor tablet), and 0.2 U/µL SUPERase-in RNAse inhibitor (Invitrogen)) ...
-
bioRxiv - Pathology 2020Quote: ... The primary antibodies applied to HK-2 cells were anti-α-smooth muscle actin (α-SMA, cat. no. A5228; 1:500; Sigma-Aldrich), anti-vimentin (cat ...
-
bioRxiv - Genetics 2020Quote: ... containing either tunicamycin only (Enzo Life-Sciences; 2-3 μg/mL) or tunicamycin and auxin (IAA, indole-3-acetic acid, Sigma-Aldrich; 1 mM), and seeded with OP50-1 bacteria ...
-
bioRxiv - Neuroscience 2020Quote: ... We incubated free-floating CeA coronal sections (16 µm) for 2 h at 30 °C with rabbit anti-FG antibody (Millipore; AB153I; 1:200) in DEPC-treated phosphate buffer (PB ...
-
bioRxiv - Genetics 2021Quote: ... eggs were fixed in 2% paraformaldehyde in PBS for 20 min and permeabilized in PBS containing 0.2% Triton X-100 (Sigma-Aldrich, 900-93-1) for 20 min ...
-
bioRxiv - Microbiology 2020Quote: ... Larvae were then moved to fresh E3 media supplemented with 0.02 mg/ml of 1-phenyl-2-thiourea (PTU) (Sigma-Aldrich, St. Louis, MO) to prevent pigmentation ...
-
bioRxiv - Neuroscience 2021Quote: ... free-floating sections were washed with 1X PBS three times (1×10 min, 2×5 min) and incubated for 10 min in 0.5% Thio-S (T1892; Sigma-Aldrich, St. Louis, MO) diluted in 50% ethanol ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were washed with FACS buffer (2% FBS and 5 mM EDTA in PBS) prior to staining with antibodies and/or Calcofluor White M2R (Sigma, 1 μg/mL) in FACS buffer for 15-20min ...
-
bioRxiv - Developmental Biology 2021Quote: ... first received ExM supplemented with 10 nM beta-estradiol for 2 days and afterwards ExM with 1 μM progesterone (Sigma, Cat. No. P8783) and 1 μM cAMP (Tocris ...
-
bioRxiv - Cancer Biology 2021Quote: ... and cultured at 37 °C for 24 h, followed by various concentrations (0, 1, 2, 5, 10 µM) of lomitapide (Sigma Aldrich, Missouri, USA). The plate was incubated at 5 % CO2 at 37 °C for 24 h after treatment with lomitapide ...
-
bioRxiv - Immunology 2021Quote: TCRδCreER.ZsGreen or TCRδCreER.TdTomato mice were fed with TAM chow for 30 days followed by normal chow and 1 mg/ml 5-bromo-2’-deoxyuridine (BrdU, Sigma, St. Louis, MO) in drinking water containing 1% glucose (to reduce the taste aversion and increase the palatability of water ...
-
bioRxiv - Immunology 2020Quote: ... PP and MLN were lysed in 2-mL tubes containing one steal bead and 1 mL PBS + 0.1% Tween20 (Sigma-Aldrich, St. Louis, USA), using a QiaTissueLyzer (Qiagen ...
-
bioRxiv - Immunology 2020Quote: ... 0.2 ml saline (0.9%) solution containing 1 mg Al (OH)3 as an adjuvant and 20 µg BLG (Sigma, St. Louis, MO, USA). Control mice received 0.2 ml saline (0.9% p/v ...
-
bioRxiv - Neuroscience 2021Quote: ... were collected in RIPA buffer supplemented with 1x cOmplete protease inhibitor cocktail as well as phosphatase inhibitor cocktail 2 at 1:100 dilution (#P5726, Sigma-Aldrich, MO, USA). Samples were stored at −70°C until analysis ...
-
bioRxiv - Microbiology 2022Quote: ... The precipitates were extracted into 1x SDS sample buffer containing 2-mercaptethanol and subjected to a standard immunoblotting assay using HRP-conjugated anti-HA antibody (H6533; Sigma; 1:3000 dilution) and anti-FLAG antibody (A8592 ...
-
bioRxiv - Cancer Biology 2023Quote: ... HD cells were washed with PBSAG (phosphate buffer saline [PBS, Sigma-Aldrich] + 1 mg/ml bovine serum albumin [BSA, Sigma-Aldrich] + 2 mg/ml glucose [Sigma-Aldrich]) and stored at 4°C for a maximum of 1 week.
-
bioRxiv - Microbiology 2023Quote: ... Lungs from immunized mice were harvested and digested for 1 h at 37°C in digestion buffer consisting of RPMI media supplemented with Collagenase (Sigma, 2 mg/ml) and DNase I (Sigma ...
-
bioRxiv - Genomics 2023Quote: The lentiviral titer was determined by transducing 400,000 cells with increasing volumes (0, 1, 2, 5, 10, 20, and 40 μl) of concentrated virus and polybrene (6 μg/ml; Millipore, TR-1003-G). 48 hours after the transduction ...
-
bioRxiv - Physiology 2023Quote: ... bundles were rinsed twice in Rigor solution containing 2 mM DTT and 1:200 dilution of protease inhibitor (Sigma-Aldrich, St. Louis, MO) before being homogenized for 1 or 2 pulses of 30s at high speed ...
-
bioRxiv - Plant Biology 2023Quote: ... PBR cultures of 1 mL were harvested in 1.5-mL tubes with 2.5 μL 2% Tween20 (Sigma, P9416-100ML, to help cell pelleting), and centrifuged at 18,407 g at 4°C ...
-
bioRxiv - Cell Biology 2024Quote: ... purified CAPS-GST protein was mixed with increasing concentrations of microtubules in Ca2+ Binding-Buffer (80 mM PIPEs, 2 mM MgCl2, 50 uM CaCl2, 1 mg/ml BSA, 10 uM leupeptin (Sigma Aldrich L-2884), 1 uM Calpain Inhibitor Peptide (Sigma-Aldrich C-9181;) ...
-
bioRxiv - Immunology 2024Quote: ... for 2 min at room temperature and the cell pellet was suspended in 1×PBS supplemented with BSA (Sigma-Aldrich, St. Louis, MO) (0.5% ...
-
bioRxiv - Molecular Biology 2023Quote: ... the two were mixed gently at a ratio of 1:2 (v/v) and resuspended in 20 times ice-cold Collagen (Sigma, Cat # C0130-1G). Cells were then aliquoted in 20 µl volumes into a 48-well plate and allowed to polymerize at 37□°C for 45Lmin ...
-
bioRxiv - Neuroscience 2023Quote: ... according to manufacturer’s instructions in dopaminergic neurons after 30 days of differentiation using 600 µl RLT lysis buffer containing 1:1,000 2-mercaptoethanol (Sigma Aldrich, cat. no M3148). After RNA extraction ...
-
bioRxiv - Microbiology 2022Quote: ... Vero E6 cells were infected with a MOI 0.002 (250 plaque forming units (PFU)) of SARS-CoV-2 for 1hr before overlaying with 1% methylcellulose- viscosity (4,000 centipoises) (Sigma- Aldrich, St. Louis, MO). Cells were incubated for 4 days at 37°C before fixing in 8% formaldehyde and stained with 1% crystal violet to visualize plaques ...
-
bioRxiv - Immunology 2022Quote: ... we inflated the lungs by injecting 1 ml of a 37 degree 2% low melting agarose solution via a catheter inserted in the trachea (Sigma-Aldrich, Cat#: A0701). We solidified the agarose by pouring a cold PBS solution (4 degrees Celsius ...
-
bioRxiv - Genomics 2023Quote: ... and cells were resuspended in 100 μL dig-wash buffer containing 2 mM EDTA and a 1:50 dilution of mouse anti-FLAG antibody (Sigma-Aldrich; F1804-1MG) and incubated at RT for 1 hour ...
-
bioRxiv - Cancer Biology 2024Quote: ... 20 ul of each sample was transferred to 2 new tubes and either and treated with 1 U/ml of hyaluronidase from Streptomyces hyalurolyticus (Sigma-Aldrich Cat # H1136) or no treatment and then incubated overnight at 37°C ...
-
bioRxiv - Immunology 2024Quote: ... then rested overnight at a concentration of 2 x 106 cells/mL in ImmunoCult™-XF T Cell Expansion Medium (StemCell; 10981) with 1% penicillin/streptomycin (Sigma-Aldrich; P0781) in a 15 cm2 dish ...
-
bioRxiv - Neuroscience 2024Quote: ... were used to calculate the amount of standard (30–70 µg) of 1-O- Heptadecyl-2-Acetyl-sn-Glycero-3-Phosphocholine (17:0 PC; Sigma-Aldrich cat# 850360P) previously diluted in 10 mL of chloroform was added as an internal standard prior to extraction ...
-
bioRxiv - Neuroscience 2024Quote: ... the membrane was washed with TBST buffer and the membrane was set for 2 hours at room temperature with the secondary goat anti-rabbit IgG HRP antibodies (Sigma Aldrich; 1:10000) diluted in a TBST buffer with 3% PVP ...
-
bioRxiv - Immunology 2024Quote: ... and 500 ng/ml Ionomycin then for a further 2 hours with the addition of 1 mg/ml Brefeldin and Monensin (all from Sigma Aldrich, Dorset, UK) in D10 medium ...
-
bioRxiv - Immunology 2024Quote: ... To assess leak by Evan’s blue the lungs were harvested and imaged with a USB desktop digital microscope (Veho) before being placed in 1 mL N,N-dimethylformamide (Sigma #68-12-2) an incubated for 4 days at 37°C to extract the dye from the tissue ...
-
bioRxiv - Biochemistry 2024Quote: ... Reactions were incubated for 30 min at 37 °C after which they were terminated by the addition of 2 μL of 1 mg/mL Proteinase K (Sigma Aldrich, cat # 3115887001), 140 mM EDTA ...
-
bioRxiv - Developmental Biology 2024Quote: ... we washed the samples with 100% methanol for 3X30 minutes and cleared the tissues overnight with benzyl alcohol: benzyl benzoate (1:2) (Sigma-Aldrich, 108006, B6630).
-
bioRxiv - Cell Biology 2024Quote: Mouse cerebella (strain C57BL/6N) were harvested at postnatal day 1 (P1) or 2 (P2) and digested with 2,5 mg/ml Trypsin (Sigma-Aldrich, cat. T1005-1G) and 1,5 mg/ml DNase (EMD Millipore Corp. ...
-
bioRxiv - Molecular Biology 2021Quote: ... The blots were incubated with primary antibodies to the following proteins overnight at 4 °C: (1) CTCF (1:1,000; 07-729, Merck Millipore), (2 ...
-
bioRxiv - Bioengineering 2020Quote: ... a gradient was prepared such that a 35% (v/v) 1:1 4-morpholinepropanesulfonic acid (MOPS):Opti-prep (Sigma) solution in HBSS was layered on top of a 20% (v/v ...
-
bioRxiv - Neuroscience 2020Quote: ... was used in 1:250 concentration to label F-actin before mounting in MOWIOL-DABCO (2.5% 1, 4-Diazabicyclo-octane (DABCO) (Sigma), 10% MOWIOL 4-88 (Sigma) ...
-
bioRxiv - Cell Biology 2021Quote: ... saturated 1 hr in 4% BSA and incubated O/N with rabbit anti-Laminin antibody (1:300, Sigma-Aldrich) to reveal myofiber outlines ...
-
bioRxiv - Pathology 2022Quote: ... Mice were administered CCl4 (1 ml/kg i.p.; diluted 1:4 in olive oil; Sigma-Aldrich, St. Louis, MO) or vehicle 2×/wk for 4 wks ...
-
bioRxiv - Developmental Biology 2021Quote: ... LIF has been omitted and derived EpiSCs can be passaged 1:4–1:10 every by triturating the colonies into small clumps using 0.5 mg ml–1 collagenase IV (Sigma). To derive mesodermal progenitors utilizing the T-eGFP FoxA2-tagRFP double knock-in reporter cell lines ...
-
bioRxiv - Genetics 2020Quote: ... incubated with primary antibodies overnight at 4°C at 1:1,000 dilution for GLI1 (Cell Signalling) or 1:10,000 dilution for β-actin (Sigma). Secondary HRP-conjugated-anti-mouse antibody was applied at a 1:2,000 dilution for 1hr at room temperature ...
-
bioRxiv - Biophysics 2021Quote: ... The slides were then mounted in 90% glycerol and 10% phosphate-buffered saline (PBS) containing 1% DABCO (1, 4-diazabicyclo-[2.2.2] octane, Sigma-Aldrich). The images and the sections were analyzed by multi-channel image acquisition tool of Fluovision software (Zeiss ...
-
bioRxiv - Cell Biology 2023Quote: ... EHTs were fixed in ice-cold 4% PFA for 1 hour and stained with anti-FLAG (Sigma, 1:1000), Alexa Fluor 568-conjugated wheat germ agglutinin (Thermo Fisher 1:1000) ...
-
bioRxiv - Neuroscience 2024Quote: ... This solution was mixed at a 1:1 ratio (v/v) with a 1:4 mixture of castor oil: sunflower seed oil (Sigma, Cat #s 259853 and S5007) to yield a final concentration of 10 mg/ml of 4-hydroxytamoxifen ...
-
bioRxiv - Bioengineering 2024Quote: ... which were grown in LB media at 37°C and 4-5 h later induced by 1 mM isopropyl β-d-1-thiogalactopyranoside (IPTG) (Sigma-Aldrich, 367-93-1) for 18-20 h at 16°C ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were then selected with 4 μg mL-1 puromycin (Sigma-Aldrich) for 3 days and expanded prior to seeding into triple flasks (Nunc® Sigma-aldrich) ...