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Citations for Millipore Sigma :
6001 - 6050 of 10000+ citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Lipids were extracted using chloroform/methanol (2:1, v/v) and shaking with an orbital shaker (IKAH VXR basic VibraxH - Sigma-Aldrich, Saint-Louis, USA) at 1500 rpm during at least 1 h ...
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bioRxiv - Biochemistry 2021Quote: ... After adding 1 drop of bovine thrombin (Biomed, Lublin, Poland) to 2 drops of fibrinogen (1mg/ml; Sigma-Aldrich, St. Louis, MO; Merck KGaA) the cells were entrapped within the fibrin clots ...
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bioRxiv - Cell Biology 2021Quote: ... for 10 mins and incubated in blocking PBS solution containing 2% BSA (AppliChem, 9048-46-8) and 0.2% Triton™ X-100 (Sigma-Aldrich, 9002-93-1) for 30 mins to permeabilize cell membrane and block unspecific antigen binding ...
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bioRxiv - Cell Biology 2021Quote: ... and were then freed from any remaining cumulus cells by a 1–2 min treatment of 0.1 % hyaluronidase (Sigma-Aldrich, St. Louis, MO, ISA) dissolved in M2 medium (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.1 mM EDTA, 10 mM KCl, 1x Complete® [Roche, 1697498], 1 mM DTT, 1x phosphatase inhibitor cocktails 2 and 3 [Sigma Aldrich, P5726/P0044]). After incubation on ice for 15 min ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 500 and 1000 μg/mL in 1 mL of extraction solution consisting of propan-2-ol with heptadecane at purity of 99% (Sigma Aldrich, cod. 128503-100G) at concentration of 50 μg/L.
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bioRxiv - Immunology 2022Quote: ... and 50 μM 2-mercaptoethanol (D10 medium) in 12 well plates in the presence of adenosine (0–1 mM) (Sigma, St. Louis, MO, USA); in the presence of each adenosine receptor agonist (0–10 μM ...
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bioRxiv - Cell Biology 2022Quote: ... The EV pellets were resuspended in 1 ml of 20 mM Tris-HCl pH 7.5 and mixed with 2 ml of 60% Optiprep™ (Sigma-Aldrich, St. Louis, MO) to create a 40% layer ...
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bioRxiv - Bioengineering 2022Quote: ... 90°C), incubation time (1 min, 2 min) and permeabilization status (with or without incubation in 0.05% Triton-X [Sigma] in PBS for 10 min) were tested (Figure 2B) ...
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bioRxiv - Biophysics 2022Quote: ... the assembled structure was coated with 100 µg.mL−1 poly-L-lysine (20 kDa) grafted with polyethylene glycol (2 kDa) (PLL-PEG) (Sigma-Aldrich, St Louis, MO, USA) for 30 min at room temperature ...
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bioRxiv - Plant Biology 2021Quote: ... 5-day-old Col-0 and lnccobra1-2 seedlings were crosslinked in PBS with 1% formaldehyde (v/v) (Sigma-Aldrich; St. Louis, MO, USA) added and placed under vacuum for 10 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... The slices were rinsed with the blocking solution and incubated with secondary antibodies for 2 h at room temperature (FITC-conjugated anti-rabbit IgG, AP132F, Millipore, Darmstadt, Germany; 1:200). The slices were then washed three times with PBS and mounted with 4’,6-diamidino-2-phenylindole (DAPI)-containing Vectashield (Vector Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... Lipids were extracted using 4 ml Chloroform (CHCl3)/methanol (CH3OH) (2:1, v/v) and shaken with an orbital shaker (IKA® VX® basic Vibrax®, Sigma-Aldrich) at 1,500 rpm for 2 h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... The thawing and culturing medium (NB Plus +) consisted of Neurobasal Plus Medium containing 2% B-27 Plus Supplement and 1% penicillin–streptomycin (PS; Sigma−Aldrich, cat. no. P0781). After thawing ...
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bioRxiv - Immunology 2022Quote: ... were cultured in complete IMDM ((IMDM [Life Technologies], 10% FBS [26140079, Life Technologies], 1% Penicillin-Streptomycin [Life Technologies], and 50mM 2-mercapto-ethanol [Sigma-Aldrich]) on plate-bound anti-CD3 (clone 145-2C11 ...
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bioRxiv - Immunology 2022Quote: ... for 2 h at room temperature and then blocked for 1 h at room temperature with 5 % neonatal calf serum (Millipore Sigma, St. Louis, MO) in PBS ...
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bioRxiv - Bioengineering 2020Quote: HDFs and hMSCs were cultured in their appropriate media compositions supplemented with 1% L-ascorbic acid 2-phosphate (AsAP, Sigma-Aldrich, A8960, the Netherlands), 15% FBS and 1% PS ...
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bioRxiv - Plant Biology 2020Quote: ... frozen leaf samples were ground in liquid nitrogen and incubated in 4 ml lysis buffer (50 mM Tris-HCl pH7.5, 150 mM NaCl, 5% glycerol, 0.05% NP-40, 1 mM MgCl2, 0.5 mM PMSF, 1X Sigma protease inhibitor, 10 mM DTT, 2% PVPP) at 4 °C for 15 min using a rotator to form a homogeneous suspension ...
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bioRxiv - Neuroscience 2020Quote: ... one containing 20% ethanol adulterated with 100 μM (Days 1 and 2) or 250 μM (Day 3) quinine hemisulfate (Sigma-Aldrich, St. Louis, MO) and the other containing water ...
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bioRxiv - Biochemistry 2020Quote: ... 2 μM FeSO4) were added in the form of crystals to D-depleted water (≤1 ppm deuterium oxide; Sigma-Aldrich, St. Louis, MO, USA), followed by the carbon source (glucose crystals or 99 % glycerol ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 500 and 1000 μg/mL in 1 milliliter of extraction solution consisting to propan-2-ol with heptadecane at purity of 99% (Sigma Aldrich, cod. 128503-100G) at concentration of 50 μg/L.
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bioRxiv - Developmental Biology 2020Quote: ... 10% fetal bovine serum in PBS) for 2 hours in room temperature and stained with anti α-Tubulin (DM1A; Sigma-Aldrich, 1:1000, overnight) followed by secondary antibody staining (Alexa Flour 488 anti-mouse 1:1000,2 hours in room temperature).
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bioRxiv - Neuroscience 2023Quote: ... light was applied for at least 2 seconds during periods of increased IPSC activity induced by a 500 ms puff of acetylcholine (1 μM, Millipore Sigma, St. Louis, MO) through a picospritzer ...
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bioRxiv - Neuroscience 2022Quote: Brain punches were homogenized in radioimmunoprecipitation assay (RIPA) buffer supplemented with phosphatase inhibitor cocktail 1 and 2 and protease inhibitor cocktail (Sigma Aldrich, Saint Louis, MO) followed by centrifugation at 16,000 g for 20 min at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated for at least 20 hours at room temperature with a combination of the following primary antibodies in PBS-TX with 2% NGS or NDS: guinea pig anti-VGLUT1 (1:1000; AB5905, Sigma-Aldrich, St. Louis, USA), guinea pig anti-VGLUT2 (1:20,000 ...
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bioRxiv - Neuroscience 2024Quote: ... The iL3 was then transferred in a single droplet to a 2% Nematode Growth Media (NGM) agar plate [67] and paralyzed with nicotine (Sigma N3876, 1% in dH2O). The iL3 was then scored for activation based on the presence (activated ...
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bioRxiv - Microbiology 2024Quote: ... pylori culture was adjusted to a concentration of 2*108 bacteria/ml in BHI supplemented with 20% FCS and was stained with TAMRA (Sigma, 1 µl TAMRA/ml) for 30 min shaking at 120 rpm in microaerobic conditions at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... the growth medium was changed to a fusion medium (DMEM supplemented with 2% heat inactivated horse serum, insulin [10 μg/ml, Sigma-Aldrich] and 1% antibiotics). Experiments were performed at the myotube stage ...
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bioRxiv - Biochemistry 2023Quote: ... 1,3(d5)-dipentadecanoyl-2-(9Z-octadecenoyl)-glycerol (15:0/18:1/15:0-d5 TG) were obtained from Sigma Aldrich (St Quentin-Fallavier, France). QReSS kit containing labelled metabolites (stable isotope-labelled molecules was obtained from Cambridge Isotope Laboratories Inc ...
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bioRxiv - Biochemistry 2022Quote: ... Primary antibody were diluted in Blocking Buffer as described below and incubated with samples for 2 hours: γ-H2AX monoclonal mouse antibody JBW301 (Millipore Sigma, 1:1000). Samples were washed with Blocking Buffer twice for 5 minutes each then treated with secondary antibody diluted 1:200 for 2 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... des-Arg9-Leu8]-BK (B1R antagonist) and 3-(5′-Hydroxymethyl-2′-furyl)-1-benzyl indazole were purchased from Sigma-Aldrich (St. Louis, MO, USA). 8-Bromoguanosine 3′ ...
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bioRxiv - Microbiology 2023Quote: ... To improve DNA recovery and avoid the interference of carbon with the extraction, 25 µL of 20 g·l−1 autoclaved (20 min, 121° Celsius, 2 bar) skimmed milk solution (Sigma Aldrich, Saint Louis, MO, USA) were added to the extraction tube ...
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bioRxiv - Bioengineering 2023Quote: ... iSMCs were incubated for 30 mins in blocking solution containing 2% BSA (AppliChem, 9048-46-8) and 0.5% Triton™ X-100 (Sigma-Aldrich, 9002-93-1). The blocking solution was then discarded and iSMCs were incubated for 2 hrs with primary antibodies diluted in blocking solution ...
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bioRxiv - Microbiology 2023Quote: ... 10 µL of the resulting supernatant was mixed with 115 µL of buffer 2 (1 mM Tris, 150 mM NaCl) both with and without 0.8 mM terbium chloride (Sigma-Aldrich, St. Louis, MI, USA). Following a 15-minute incubation ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The solution in each well was changed every 1-2 days with FSW containing 60□μg/mL penicillin and 50□μg/mL streptomycin (Sigma-Aldrich # P3032 and #S6501).
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bioRxiv - Neuroscience 2024Quote: ... Cortex samples were placed in a 2 mL Dounce homogenizer on ice with 1 mL ice cold Nuclei EZ lysis buffer (Sigma, #EZ PREP NUC-101) and homogenized 15-20 times with pestle A and then 15-20 times with pestle B ...
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bioRxiv - Microbiology 2024Quote: THP-1/PMA macrophages were infected with single-cycle HIV-1 reporter viruses (MOI 2) in presence of polybrene (10 μg/mL, Millipore Sigma, TR-1003-G) via spinoculation (2,300 rpm ...
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bioRxiv - Neuroscience 2024Quote: ... cells were incubated for 1 hr in Fish Gel Blocking Buffer (2% Fish Gel [Sigma-Aldrich G741-100G], 0.2% Triton-X [Sigma-Aldrich T9284] in PBS) at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Ref#322000) for 2 min and then rinsed in water before incubation with 1 µg anti-centrin primary antibody (Millipore Sigma, Cat# 04-1624) overnight at 4°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1×106 cells (n=2) were collected per sample and transferred into a Nunc MicroWell 96-well polypropylene plate (Millipore Sigma, cat#P6866-1CS) for staining ...
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bioRxiv - Neuroscience 2024Quote: ... with cold PBS before being blocked for 1 hr in fish Gel Blocking Buffer (2% Fish Gel [Sigma-Aldrich G741-100G], 0.2% Triton-X [Sigma-Aldrich T9284] in PBS) at room temperature ...
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bioRxiv - Genomics 2024Quote: ... This mixture of plasmid DNA was cautiously introduced dropwise into 1 mL of filtered 2x concentrate BES buffered saline (Millipore 14280, bioWORLD 40220006-2), while gently inducing air bubbles through the DNA mixture using a sterile 1 mL pipette ...
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The RIFF: an automated environment for studying the neural basis of auditory-guided complex behaviorbioRxiv - Neuroscience 2021Quote: ... and 1:1 mixture of 4% sucrose and 0.1 % saccharine solutions (sucrose and saccharin, Sigma-Aldrich, St. Louis, MO, USA).
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Frozen brain tissue was ground with a motorized pestle for 30 s in 100 µl of chilled (4:1) analytical grade methanol:ddH2O with 1 mM norluecine (Sigma Aldrich) standard ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated overnight at 4°C with guinea pig anti-vGlut2 antiserum (1:12,000, cat# AB2251-1, Millipore Sigma) in PBS-T containing 2% normal donkey serum ...
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bioRxiv - Microbiology 2021Quote: ... Cultures were then diluted 1:100 in prewarmed minimal media A containing 4 µg mL-1 polymyxin B sulfate (Sigma). Samples were incubated at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated 1-3 overnights at 4°C in the following primary antibodies: goat anti-choline acetyltransferase (1:200; Millipore), rabbit anti-substance P (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... blots were blocked for 1 h and incubated overnight at 4 °C with anti-Amyloid fibrils (OC, 1:5000, Merck-Millipore), anti-prefibrillar oligomers (A11 ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were centrifuged for 5 min at 20 000 x g and supernatants were immunoprecipitated for 1 h rotating at 4°C with mouse anti-GFP antibody (1 µg antibody per sample, 11814460001 Merck/Sigma) and 20 µl DynabeadsTM Protein G (10004D ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were incubated overnight at 4 °C with the primary antibodies: mouse anti-Syntaxin-1 (1:500, Sigma, # S0664), chicken anti-GFP (1:3000 ...