Labshake search
Citations for Millipore Sigma :
5951 - 6000 of 6010 citations for Resistant Starch Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... the nuclear fraction was prepared from whole plants using the ‘Semi-pure Preparation of Nuclei Procedures’ protocol of the CelLytic PN Isolation/Extraction Kit (Sigma-Aldrich, St. Louis, MO). Genomic DNA was isolated from the nucleus or whole plant using a DNeasy Plant mini kit (Qiagen ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... determined as the amount of 5-bromo-2′-deoxy-uridine (BrdU) incorporation (Roche BrDU Labeling and Detection Kit II, Sigma-Aldrich, St. Louis, MO). Briefly ...
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bioRxiv - Developmental Biology 2022Quote: ... offspring were screened for Akt1 or Foxo4 mutations from genomic DNA from tail-tip biopsies using the REDExtract-N-Amp™ Tissue PCR kit (XNAT, Millipore Sigma, Burlington, MA). PCR was performed on the purified DNA samples using primers flanking the guide RNA sites ...
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bioRxiv - Plant Biology 2022Quote: ... Full-length FIN4 mRNA in PCRII-TOPO vector was used to synthesize sense and anti-sense probes using a DIG-labeled probe synthesizing kit (Millipore-Sigma, catalog number 11175025910) with T7 RNA polymerase for sense probe and SP6 RNA polymerase for antisense probe synthesis with 1μg plasmid as template ...
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bioRxiv - Microbiology 2024Quote: PCR products obtained from the first round of amplification (COMPETE, BLOCK, CONTROL, see section 2.2) were purified using the GenElute™ PCR Clean-Up Kit (Sigma Aldrich, St. Louis, MO). The purified DNA was diluted 10-fold with DNA free sterile water and used as a DNA template for a nested PCR to attach Illumina adaptors for Illumina sequencing ...
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bioRxiv - Physiology 2023Quote: 25-30 mg of frozen powdered liver tissue was used to extract liver lipids as described previously 34 and reconstituted in a tert-butanol-Triton X solution (3:2) and liver triglycerides and cholesterol levels were measured via a commercially available kit (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Immunology 2024Quote: Total RNA was extracted from endothelial cells with the TRIzol reagent and Poly(A)+ RNA was purified using the GenEl-ute mRNA Miniprep Kit (Sigma, Louis, MO, USA; MRN10). The m6A concentration was assessed using the m6A RNA Methylation Kit (Abcam ...
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bioRxiv - Neuroscience 2023Quote: The TUNEL assay was performed on paraffin-embedded sections and fifty-micron-thick free-floating frozen sections according to the manufacturer’s instructions but with the time of some steps lengthened to ensure good penetration of the reagents into the tissue (ApopTag® Fluorescein In Situ Apoptosis Detection Kit, Millipore-Sigma, Cat # S7110). Sections were post-fixed for ten minutes at room temperature in 2:1 ethanol:acetic acid ...
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bioRxiv - Immunology 2023Quote: Cryopreserved blood plasma was thawed at room temperature and used for IL17a detection by a commercial mouse IL-17a ELISA Kit (Sigma Aldrich catalog #RAB0263-1KT). The test was performed according to manufacturer guidelines ...
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bioRxiv - Biophysics 2023Quote: ... were grown in ATCC’s vascular cell basal medium supplemented with microvascular endothelial cell growth kit-VEGF and 12.5 μg/mL blasticidine (Sigma-Aldrich, St. Louis, MO, 203351) for 48 h at 37°C + 5% CO2 until reaching 80-90% confluency ...
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bioRxiv - Plant Biology 2023Quote: RNA was extracted from leaf samples (three biological replicates for each genotype) and using Spectrum Plant Total RNA Kit (Sigma Aldrich, Saint Luis, USA) following instruction ...
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bioRxiv - Microbiology 2023Quote: ... bile salts were supplied from bovine bile and measured using a commercial kit according to the supplier’s protocol (Sigma-Aldrich, cat. No. MAK 309). Finally ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue lysis was performed in Roche cOmplete Lysis-M reagents (lysis buffer and protease inhibitor cocktail contained in the kit, Millipore Sigma cat. Nr. 4719956001). Hippocampi were placed in protein low-binding tubes mounted onto a Qiagen TissueLyser II (Qiagen cat ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein solution was dialyzed overnight at 4°C in tubes of the Pur-A-LyzerTM Maxi 6000 Dialysis Kit (Sigma, cat. No. PURX60100-1KT) against dialysis buffer (20 mM Tris ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from 1.5-week-old Arabidopsis seedlings pooling 3 seedlings/extraction for each transgenic lines and using Spectrum™ Plant Total RNA Kit (Sigma-Al-drich, STRN250), RNAs were on-column treated with the DNase (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... 16 were subjected to in-situ Terminal deoxynucleotidyl Transferase dUTP Nick End Labeling (TUNEL) using the ApopTag Plus Fluorescein Kit (EMD Millipore, Burlington, MA, USA), following previously established protocols (Maddala et al. ...
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bioRxiv - Immunology 2021Quote: ... At 0 min and 1 h the sample was analyzed for concentrations of inorganic monophosphates with the ATPase/GTPase Activity Kit (Sigma-Aldrich, Cat. no: MAK113-1KT) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... The PCR amplification for each gene sequence was performed with 50 ng of genomic DNA in a 20 µL reaction using a KAPA Taq PCR Kit (Sigma-Aldrich, St Louis, MO, USA) and the following cycle conditions ...
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bioRxiv - Plant Biology 2021Quote: ... 5’ RACE PCR was performed with 2 µl of 1:5 diluted cDNA in a 20 µl reaction with the KAPA2G Robust PCR Kit (KK5501, Sigma-Aldrich, St. Louis, Missouri, USA) buffer B ...
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bioRxiv - Plant Biology 2020Quote: ... they were dehydrated in an ethanol series (25, 50, 70, 100, and 100 %) and embedded in Epoxy resin using Epoxy medium kit (Sigma-Aldrich, St-Louis, MO, USA). Then ...
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bioRxiv - Genetics 2022Quote: ... Genomic DNA was extracted from pooled seedlings at the EBI laboratories using the GenElute Plant Genomic DNA Miniprep Kit (Sigma-Aldrich, St. Louis, MO, USA) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: RNA was prepared for Iso-Seq using the Sigma Spectrum Plant Total RNA Kit including On-Column DNAse I Digestion (both products Sigma-Aldrich, St. Louis, MO, USA). One Hen17-A07 plant was sectioned into three parts (roots ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... ground and homogenized western corn rootworm larval tissue (3-7 larvae per biological replicate, n=8-11) using the GenElute Universal Total RNA Purification Kit (Sigma-Aldrich, St. Louis, MO, USA). A NanoDrop spectrophotometer (ND-1000 ...
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bioRxiv - Neuroscience 2021Quote: Genomic DNA was extracted from tail tissues and amplified with specific PCR primers using REDExtract-N-Amp™ Tissue PCR kit (Cat. No. XNAT-100RXN, Sigma-Aldrich, Saint Louis, MO) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue digestion for DNA extraction and subsequent polymerase chain reaction (PCR) was performed with the prepared sample buffer from REDextract-N-Amp PCR KIT (Sigma-Aldrich, St. Louis, United States) or DreamTaq™ Hot Start Green DNA Polymerase (Thermo Fischer Scientific ...
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bioRxiv - Synthetic Biology 2022Quote: Genomic DNA of the evolved mutants was isolated from LB overnight cultures using the GenElute™ Bacterial Genomic DNA Kit (Sigma-Aldrich St. Louis, MO). The extracted DNA was evaluated by gel electrophoresis and quantified by a NanoDrop spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2020Quote: ... Quantitative Real-time PCR reactions were performed on a Bio-Rad CFX96 Termo-cycler with Kapa Syber Fast qPCR master mix (2x) kit (Sigma-Aldrich, Saint Louis, MO, USA), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... The nuclear extract was prepared from larval tissue (~20 larvae) of Canton-S stock using the CelLytic™ NuCLEAR™ Extraction Kit (Sigma, Missouri, Unites States). The nuclear extract was taken in different amounts and incubated in the presence of 1X binding buffer (250mM HEPES ...
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bioRxiv - Cell Biology 2020Quote: The PLA assay was performed to determine the in situ binding between LC3 and ATG9a or ATG9b by using the Duolink® In Situ Red Starter Kit purchased from Sigma Aldrich (catalog number DUO92101), following the protocol provided by the manufacturer.
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bioRxiv - Genomics 2020Quote: ... Supernatant was aspirated before the isolation of single nuclei was performed using the Nuclei PURE Prep nuclei isolation kit (Sigma-Aldrich, St. Louis, MO, USA) in conjunction with a modified protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: Total RNA of the collected/dissected petals (see supplementary fig. S2) was extracted using Spectrum Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA). cDNA was synthesized following the methods described in Supporting Information Methods S4 in Lin and Rausher (2021) ...
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bioRxiv - Microbiology 2019Quote: ... The supernatant was collected avoiding to disturb the pellet and was mixed with 750 microliters of Binding buffer of Total Spectrum RNA kit (Sigma–Aldrich, Saint Louis, MO, USA). Subsequently the extraction proceeded following manufacturer instructions.
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bioRxiv - Microbiology 2021Quote: RNA extract from a single female adult tick was converted to cDNA using the Complete Whole Transcriptome Amplification Kit (WTA2, Sigma-Aldrich, Saint Louis, MO, USA). Purification of cDNA was done using the MSB Spin PCRapace kit (Stratec Molecular ...
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bioRxiv - Microbiology 2020Quote: ... All transformants were screened by PCR using gene-specific primers (Table S2, Fig. S2c) and were verified by Southern hybridization analyses (DIG DNA Labeling and Detection Kit, Sigma-Aldrich, St. Louis, MO, USA) (Fig ...
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bioRxiv - Immunology 2021Quote: ... Vero E6 monolayers are washed and incubated for 5-6 days at 37 degrees after which plates are scored using the vitality marker WST-8 (colorimetric cell counting kit, Sigma Aldrich, cat 96992-3000TESTS-F). To this end ...
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bioRxiv - Bioengineering 2020Quote: Plasmid DNA was purified after overnight culture in standard lysogeny broth containing 100 µg/mL ampicillin using a miniprep plasmid DNA isolation kit (Sigma Aldrich, St Louis, MO; PLN350) and quantified using spectrophotometry (NanoDrop One ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was extracted from 21 days-after-flowering (DAF) sunflower seeds using the Spectrum Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: Tissues were ground into a fine powder in liquid nitrogen and total RNA was extracted using the Spectrum™ Plant Total RNA kit (Sigma-Aldrich, St. Louis, MO). Sequencing libraries were produced using the TruSeq RNA Sample Preparation kit v2 (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 mg of leaf powder was used for RNA extraction following protocol A of the Spectrum™ Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO). RNA was eluted in 50 μL elution buffer ...
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bioRxiv - Plant Biology 2022Quote: Harvested tissue was grounded to a fine powder in liquid nitrogen and RNA was extracted using the Spectrum Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA) following the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from 100 mg of frozen and ground samples using the Spectrum™ Plant Total RNA Kit (Sigma, St. Louis, Missouri, USA) according to the manufacturer’s Protocol A ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was extracted from frozen samples in the manual of the GenElute Bacterial DNA Isolation kit (Sigma Aldrich, NA2110-1KT, St. Louis, MO, USA) with the following minor modifications as previously described60 ...
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bioRxiv - Genetics 2024Quote: RNA was extracted from 100 mg of frozen ground leaves per replicate using the Spectrum Plant Total RNA kit (Sigma-Aldrich, St Louis, MO, USA) followed by treatment with RNase-free DNase using the On-Column DNase I Digestion Set (Sigma-Aldrich) ...
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bioRxiv - Genetics 2024Quote: ... quantification in the heads of both starved and non-starved miR-210 KO and WT flies was carried out using the Triglyceride Quantification Kit (Sigma-Aldrich, St. Louis, Missouri, USA), following the provided manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were isolated from snap-frozen frontal cortex (anterior to the anterior forceps of the corpus callosum) samples from one hemisphere using Nuclei Isolation Kit: Nuclei EZ Prep (Sigma-Aldrich Cat. No. NUC-101). Briefly ...
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bioRxiv - Plant Biology 2023Quote: The total RNA was extracted from PEG-treated (24 h) and untreated control (0 h) samples using Spectrum plant total RNA Kit (Sigma Aldrich, St. Louis, MO, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was extracted from a tail biopsy and genotypes were determined using the Extract-N-Amp Tissue PCR kit (Sigma-Aldrich, St Louis, MO, USA). The following primer sequences (Integrated DNA Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... the first step was to remove the iodixanol through dialysis using Pur-A-LyzerTM Mini 6000 dialysis kit (PURN60030, Sigma-Aldrich, Co., STL, MO, USA). Then SEC-DGUC-1 was subjected to in-solution digestion prior to fractionation on an Xbridge™ C18 column (4.6 × 250 mm ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we grinded the plant tissue into a fine powder with a pestle and a mortar in the presence of liquid nitrogen and processed the samples using the Spectrum™ Plant Total RNA-Kit from Sigma-Aldrich (product number: STRN250), including on-column DNA digestion using the on-column DNAse kit (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... To detect Edu signal was used “EdU IV Imaging Kit 647 S” based on the Click-it reaction with azides conjugated to fluorochromes (Sigma Aldrich, BCK647-IV-IM-S) following instructions of the manufacturer.