Labshake search
Citations for Millipore Sigma :
5851 - 5900 of 7633 citations for Yellow Fever Virus NS1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... The separated proteins were transferred to PVDF membranes and blocked in PBS containing 0.2% fish skin gelatin (Sigma-Aldrich) and 0.1% Tween-20 ...
-
bioRxiv - Microbiology 2020Quote: ... the mice were injected with 700 μg of His-tagged TGT complex protein in incomplete Freund’s adjuvant (Sigma-Aldrich). One week after the 6-week injection ...
-
bioRxiv - Biochemistry 2021Quote: ... After elution with imidazole the purified protein was concentrated using a 10 kDa MW cutoff Amicon centrifugal filter (Sigma), buffer exchanged into PBS (10mM Na2HPO4 ...
-
bioRxiv - Cancer Biology 2021Quote: ... The protein pellet was washed 3 times in a solution of 0.3 M guanidine hydrochloride (Sigma, Cat. No. SRE0066) in 95% ethanol ...
-
bioRxiv - Neuroscience 2019Quote: ... proteins were reduced with 20 mM dithiothreitol and free cystein residues were alkylated with 50 mM iodoacetamide (Sigma Aldrich). After the urea washing steps ...
-
bioRxiv - Biochemistry 2021Quote: ... The supernatant containing Galectin-3 protein was treated with benzamidine sepharose (HiTrap Benzamidine FF, Sigma, Cat# GE17-5143-02) to bind and remove thrombin ...
-
bioRxiv - Microbiology 2021Quote: ... Antibodies to the N protein was detected by incubation for 1h at 37°C with anti-human IgG peroxidase (1:30,000, Sigma). The plates were washed three times with PBS with 0,05% Tween-20 between each step ...
-
bioRxiv - Developmental Biology 2020Quote: ... the supernatant was subjected to an immunoreaction with a specific antibody pre-conjugated with magnetic protein A beads (Millipore) overnight at 4°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were blocked with 3% w/v BSA and incubated with 1:16,000 v/v rabbit anti-protein A antibody (SIGMA) for 1 h at room temperature ...
-
bioRxiv - Molecular Biology 2019Quote: ... Fractions containing the complex were concentrated to 0.12 mg/ml and proteins were crosslinked by addition of 0.05% (v/v) glutaraldehyde (Sigma Aldrich) and incubated at room temperature for 60 min ...
-
bioRxiv - Molecular Biology 2019Quote: ... Extracted proteins were then separated via SDS-PAGE and then transferred to a 0.45 μM polyvinylidene fluoride (PVDF) membrane (Millipore) on a TRANS-BLOT SD apparatus (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Total protein content in each eluate was measured using infra-red spectroscopy (Direct Detect, Merck Millipore, Burlington, MA, USA). The remainder was aliquoted and stored at −80 °C.
-
bioRxiv - Molecular Biology 2020Quote: ... equal amounts of total protein were separated by 10% SDS-PAGE and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore). The membranes were blocked and incubated with primary antibodies against FGF2 (36769 ...
-
bioRxiv - Molecular Biology 2020Quote: ... the His6-tag-fusion proteins were purified from the supernatant by a HIS-Select® Nickel Affinity Gel (Sigma) column ...
-
bioRxiv - Biochemistry 2021Quote: ... Peak fractions corresponding to soluble protein were pooled and concentrated to 4.5 - 5.5 mg/mL (Amicon Ultra 100 kDa cut off, Millipore Sigma). Protein samples were flash-frozen in liquid nitrogen and stored at −80°C.
-
bioRxiv - Microbiology 2020Quote: ... For elution of FLAG-tagged proteins and their complexes 100-300 µL of 0.1 mg/mL FLAG3 peptide (Sigma) was added to samples ...
-
bioRxiv - Cancer Biology 2019Quote: ... a sample mixture of 10 μg protein and non-reducing sample buffer was loaded on a polyacrylamide gel containing gelatin (0.25 mg/ml; Sigma). The gel was then soaked (36 h ...
-
bioRxiv - Immunology 2020Quote: Western blot analysis of protein levels was performed on whole cell lysates prepared by lysis in RIPA buffer (Sigma) supplemented with Complete EDTA free proteinase inhibitors (Roche) ...
-
bioRxiv - Cancer Biology 2022Quote: ... the protein extracts (15 μg/sample) were blotted on a primed 0.45 μm polyvinylidene fluoride (PVDF) membrane (Merck-Millipore). Protein spots were stained using Direct Blue (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2022Quote: ... The proteins samples (20 μg/lane) were separated by SDS–PAGE and transferred onto PVDF membranes (0.45μm, Millipore, USA) using a transfer unit (Bio–RAD ...
-
bioRxiv - Genomics 2022Quote: ... Proteins from whole cell lysate were separated in Laemmli buffer by SDS-PAGE and transferred to PVDF membranes (Millipore). Membranes were blocked in 5% milk in Tris-buffered saline with 0.1% Tween-20 (TBST ...
-
bioRxiv - Immunology 2022Quote: ... Protein lysates were separated on 10% SDS-PAGE gels and then transferred to PVDF membranes (Millipore, Billerica, MA, USA). The membranes were blocked with 5% nonfat dry milk at room temperature for 2 hours and then incubated at 4°C overnight with primary antibodies against β-tubulin (Abcam ...
-
bioRxiv - Immunology 2022Quote: ... DENV infection was then measured by intracellular detection of the E protein using the murine monoclonal 4G2 antibody (Millipore) followed by rabbit anti-mouse IgG-coupled to AF647 (Molecular probes) ...
-
bioRxiv - Cancer Biology 2022Quote: ... equal protein amounts were collected across the samples and subjected to 25 μM DBCO-PEG4-biotin (Sigma-Aldrich, #760749) at room temperature for 30 min ...
-
bioRxiv - Cell Biology 2022Quote: ... The glass surfaces were coated with 1 mg/mL of BSA protein (Albumin, biotin-labeled bovine, A8549 Sigma-Aldrich) by incubating for 2 hours ...
-
bioRxiv - Neuroscience 2022Quote: Mouse monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody (diluted 1:200; cat. no. G3893; Sigma-Aldrich, MO, USA) and Goat anti-Mouse IgG (H+L ...
-
bioRxiv - Cell Biology 2022Quote: The coverslips now containing fixed spheroplasts were incubated in protein crosslinking prevention solution (2% acrylamide (AA, Sigma, cat.no. A4058) / 1.4% formaldehyde (Sigma ...
-
bioRxiv - Molecular Biology 2022Quote: ... Samples were resolved on 12% SDS–PAGE and proteins were transferred to methanol-activated polyvinylidene fluoride (PVDF) membrane (Millipore) using anode buffer (25 mM Tris HCl pH 10.4 ...
-
bioRxiv - Biochemistry 2019Quote: The proteins in an SDS-polyacrylamide gel were transferred onto an Immobilon®-P PVDF transfer membrane (Millipore Sigma) by electroblotting at 250mA for two hours in ice cold transfer buffer (25mM Tris ...
-
bioRxiv - Plant Biology 2019Quote: ... and bound protein was eluted with 60 μL of 0.5 mg/mL 3×FLAG peptide (Sigma-Aldrich, MO, USA) for 1 h at 4°C ...
-
LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: ... The lysates were then precleared for 1h at 4°C using 10µl protein A agarose 50% slurry/plate (Millipore), and collected by centrifugation at 300g for 3min at 4°C (100µl of sample/plate was kept as input chromatin) ...
-
bioRxiv - Physiology 2019Quote: ... FLAG-tagged protein were eluted twice with 200 ng/μL FLAG peptide (Sigma, #F3290; 1 h at 4°C). PL transfer activity was assayed using NBD labeled Phosphoethanolamine ...
-
bioRxiv - Cell Biology 2020Quote: ... The reaction was allowed to proceed by rotation at 4°C for 2hrs and then immunoprecipitated with 60μl Protein G Sepharose (Sigma). The sediments were washed twice with HEPES/KCl buffer + 0.25% Triton-X100 ...
-
bioRxiv - Cancer Biology 2019Quote: ... The collected lysate proteins were then subjected to immunoblotting with LC3 (L7543) and β-actin (A5441) antibodies (Sigma-Aldrich).
-
bioRxiv - Microbiology 2019Quote: ... Expression of MxA proteins was detected by Western blot analysis using mouse ANTI-FLAG® monoclonal antibody F4042 (Sigma) and rabbit anti-beta-actin polyclonal antibody ab8227 (Abcam ...
-
bioRxiv - Cell Biology 2019Quote: ... Fractions containing monomeric protein were pooled and concentrated to around 50 μM using Amicon Ultra-0.5 ml Centrifugal Filters (Millipore), and then aliquoted and snap-frozen until further use.
-
bioRxiv - Biochemistry 2019Quote: ... and proteins were resuspended in 100 μL of 100 mM ammonium acetate and 1 μg trypsin (Proteomics grade, Sigma). Proteins were digested by incubation at 37 °C for 16 h.
-
bioRxiv - Cell Biology 2019Quote: ... lysates were immunoprecipitated with ACC antibody (CST) or ACC2 antibody (CST) and EZview Red Protein G Affinity Gel (Sigma).
-
bioRxiv - Developmental Biology 2020Quote: Cells were seeded onto glass coverslips coated with 5 μg/mL human plasma fibronectin purified protein (Millipore Sigma #FC010). At ~90–100% confluency ...
-
bioRxiv - Cancer Biology 2019Quote: ... activity in each cell line was assayed as described30 using 6.7 µg of WCE proteins in the presence or absence of 300 µM of CES2 inhibitor loperamide (LOP, Sigma) in 20 µL of 100 mM Tris-HCl ...
-
bioRxiv - Developmental Biology 2019Quote: ... proteins were transferred to a PVDF membrane and immunoblotted with an anti-phosphotyrosine antibody (1:1000 dilution, Upstate/Millipore) for chemiluminescence detection.
-
bioRxiv - Plant Biology 2019Quote: ... a C-terminal polypeptide encompassing the last 284 amino acids of the PKL protein has been expressed in fusion with a hexahistidine tag in the E.coli strain BL21DE3 Rosetta (Novagen). The protein was purified under denaturing conditions on Ni2+-nitrilotriacetic acid matrix (Ni-NTA Agarose ...
-
bioRxiv - Neuroscience 2019Quote: ... The proteins were then precipitated using acetonitrile (400 µL) containing 0.1% v/v formic acid (LC-grade, Sigma-Aldrich). This was done in metal and glass syringes to ensure no contamination from plastic ...
-
bioRxiv - Cell Biology 2020Quote: ... The reaction was initiated by the addition of 6 μM vitamin D binding protein [DBP] (Human DBP, G8764, Sigma) directly in the fluorometric cuvettes ...
-
bioRxiv - Pathology 2020Quote: ... cell lysates containing equal protein amounts were separated by SDS-PAGE and transferred to PVDF membranes (Cat.#ISEQ00010, Millipore). Membranes were sequentially probed with primary antibodies and appropriate peroxidase-conjugated secondary antibodies ...
-
bioRxiv - Molecular Biology 2020Quote: ... and 4.2 μg PARG (in-house) per 10 mg protein and 10 μl of Benzonase (Merck Millipore, Darmstadt, Germany) per 30 ml were then added to each sample ...
-
bioRxiv - Molecular Biology 2020Quote: ... Binding and crosslinking of the GST-Af1521 fusion proteins to Glutathione Sepharose 4B beads (Sigma Aldrich, Saint Louis, Missouri), peptide enrichment (“standard workflow” ...
-
bioRxiv - Biophysics 2020Quote: ... 200 μL of 50 μM protein aliquots were reacted with 50 molar equivalents of methyl 2,5-dibromopentanoate (Sigma Aldrich) for 12 hours at 37 °C and shaking at 500 rpm ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 12 mg RBD protein in 3 mL PBS was mixed with equal-volume Freund’s incomplete adjuvant (Sigma-Aldrich) for the second boosting ...
-
bioRxiv - Molecular Biology 2020Quote: ... One milligram of total cellular protein was then incubated with 30 μl bed volume of anti-V5 beads (Sigma), which were pre-equilibrated in buffer A ...