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Citations for Millipore Sigma :
6051 - 6100 of 7633 citations for Yellow Fever Virus NS1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Astroglia proliferate upon biogenesis of tunneling nanotubes and clearance of α-synuclein toxicitiesbioRxiv - Cell Biology 2023Quote: ... Then the protein was labelled adding two-fold molar excess of TetramethylRhodamine-5-maleimide (TMR-maleimide; Sigma Aldrich #94506) in 20 mM HEPES buffer (pH7.4) ...
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bioRxiv - Microbiology 2023Quote: ... β-mercaptoethanol (99%) for 6 h at 4 ℃. The proteins were concentrated by Amicon Ultra (M.W. 3,000 Da) (Sigma-Aldrich) and stored at −80 ℃.
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bioRxiv - Molecular Biology 2023Quote: ... and the eluted proteins were subjected to Western Blot analysis using a GST antibody (1:1000, Millipore 05-782).
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bioRxiv - Biochemistry 2023Quote: ... Y730F)41 was cloned into pET28a vector incorporating a His6-tag and a SUMO tag at the amino terminus of the expressed protein and expressed in E.coli BL21 gold strain (Novagen). Cells were grown at 37°C in TB medium containing 0.1 mg/ml kanamycin and 0.050 mg/ml tetracyline to an OD600 of ∼3.0 ...
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bioRxiv - Cell Biology 2023Quote: ... Infected CAD5 cells were seeded in ELISPOT plates (multiscreen 96-well filter plates with high protein-binding Immobilon-P membrane, 0.45 µm, Millipore) activated with 70% ethanol ...
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bioRxiv - Biochemistry 2023Quote: ... Protein expression and native purification was performed by using His-select Nickel affinity gel resin column (Sigma Aldrich, USA) according to the manufacture’s instruction ...
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bioRxiv - Biochemistry 2023Quote: ... followed by resuspension of the dry pellets by vortexing (≈1 min) and subsequent addition of 150 μL lysis buffer (HEPES buffer containing 0.35X BugBuster Protein Extraction Reagent (Novagen) and 0.1% Lysonase Bioprocessing Reagent (Novagen)) ...
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bioRxiv - Biophysics 2023Quote: ... a solution of 50 μM αS and 1 μM N-protein in 20 mM Tris buffer (Sigma-Aldrich, UK), pH = 7.4 ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of total proteins (20μg) were separated by SDS-PAGE and transferred to a 0.45-mm Immobilon-P PVDF Membrane (Millipore, Merck) and the membrane was incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... and Tissue Protein Extraction Reagent (Pierce, Waltham, MA, USA) supplemented with 2.5% of 2-mercaptoethanol (Sigma Aldrich, Darmstadt, Germany). Cell lysates were boiled for 8 min and stored at − 80°C ...
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bioRxiv - Cancer Biology 2022Quote: ... The total protein was then resolved on SDS-PAGE (8-12%) and transferred onto PVDF membrane (Millipore, Massachusetts, USA). After the transfer ...
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bioRxiv - Biophysics 2022Quote: ... The eluted protein was buffer exchanged with PBS (pH 7.4) and was concentrated using 10 kDa cut off Amicon (Merck Millipore) concentrator to a final concentration of 1 mg/ml and stored at −80°C until further use.
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bioRxiv - Immunology 2023Quote: ... Culturing and induction of protein expression following application of the inductor isopropyl-β-D-thiogalactopyranoside (IPTG; Sigma, Deisenhofen, Germany)) was performed as previously described (33).
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bioRxiv - Biochemistry 2023Quote: ... Protein complex-containing fractions were collected and concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore). The concentrated protein was split into two samples ...
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bioRxiv - Biochemistry 2023Quote: ... MWRA flow-through protein solution was concentrated to ∼15 mL using a 30 kDa MWCO centrifugal filter (EMD Millipore), ensuring not to concentrate to where solution became yellow/cloudy and viscous ...
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bioRxiv - Biochemistry 2023Quote: 50 µg of refolded TREM2 protein in 100 mM ammonium bicarbonate buffer was digested with 2 µg trypsin (Sigma) at 37 °C for overnight ...
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bioRxiv - Neuroscience 2023Quote: Transduced CHO-K1 cells expressing hα7-nAChR and chaperone proteins were cultured on 50µg/ml poly-D-lysine (Sigma-Aldrich) and 330µg/ml Collagen type 1 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were then spun at 21,000 g for 15 min at 4°C and protein concentration determined using Bradford assay (Sigma). Approximately 3-4mg of total cell extract was mixed with 20μl of M2-anti-FLAG agarose beads (bed volume ...
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bioRxiv - Biochemistry 2023Quote: ... the protein (with N-terminal His6 tag) was concentrated using a centrifugal filter (Amicon Ultra, MWCO 30 kDa, Sigma) to 8.11 mg/ml ...
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bioRxiv - Immunology 2023Quote: ... Proteins were separated by SDS-polyacrylamide gel electrophoresis and transferred onto a nitrocellulose membrane (Amersham Protran, GE10600001, Sigma Aldrich) by the wet transfer method ...
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bioRxiv - Immunology 2023Quote: ... membrane at the bottom (Multiscreen HTS 0.45 μm Hydrophobic High Protein Binding Membrane 96-well Filtration plate (Product No. MSIPS4510; Millipore-Sigma) (65 ...
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bioRxiv - Biophysics 2023Quote: ... The calibration was performed using a protein standard mixture: of β-amylase (Sigma-Aldrich, 56, 112, and 224 kDa), and thyroglobulin (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were then transferred to a nitrocellulose membrane and blocked with PBS + 0.1% Tween 20 + 5% Milk powder (Sigma) and incubated overnight with primary antibodies at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... These lysates were then additionally treated for protein precipitation with 3 volumes of ultra-pure methanol (Sigma-Aldrich, USA). Ultimately ...
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bioRxiv - Biochemistry 2022Quote: ... HMCES-3flag protein was detected by immunoblotting with anti-flag antibody (1:10,000 dilution in PBST; Sigma, F1804-200UG) and rat anti-mouse secondary antibody peroxidase conjugate (1:20,000 dilution in PBST ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were purified using Amicon Pro Affinity Concentration Kit Protein A with 10kDa Amicon Ultra-0.5 Device (Millipore-Sigma). Antibody concentrations were determined using an IgG ELISA ...
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bioRxiv - Biophysics 2023Quote: ... As per the manufacturer’s recommendation a protein standard curve was first measured using Bovine Serum Albumin (P 0384, Sigma) standards ...
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bioRxiv - Cell Biology 2023Quote: ... 10 μg of soluble proteins in TEAB buffer were added onto 30 kDa cutoff filter (Sigma-Aldrich, cat#MRCF0R030) and centrifuged at 11,000 g for 15 minutes at RT ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were eluted with 200 µL assay buffer supplemented with 0.6 mg∕mL 3× Flag® peptide (Sigma Aldrich), and analysed by Western blotting.
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bioRxiv - Biochemistry 2023Quote: ... proteins were imaged using Immobilon Classico Western HRP substrate or Immobilon Ecl Ultra Western HRP substrate (Millipore, MA, USA) on the iBright FL1500 Imaging System (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... and secreted proteins in media were harvested after 120 hours and cleared using a 0.45-µm PVDF vacuum filter (Sigma Millipore). Each His-tagged protein in media was captured with Ni-NTA resin (Qiagen ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteins were separated using sodium dodecyl-sulfate polyacrylamide gel electrophoresis and electrophoretically blotted onto microporous polyvinylidene fluoride membranes (Millipore) under a constant current ...
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bioRxiv - Molecular Biology 2023Quote: ... Pure protein-containing fractions were pooled and concentrated using centrifugal spin concentrators with an MWCO of 30 kDa (Millipore). Finally ...
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bioRxiv - Biochemistry 2024Quote: ... and expression of the GFP-tagged protein was verified by western blot using an anti-GFP antibody (Sigma-Aldrich). Colonies with the highest expression for a given construct were used to inoculate 300 mL of YEPD media containing 200 µg/mL Zeocin and grown overnight with shaking (220 rpm ...
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bioRxiv - Microbiology 2023Quote: ... 4µg of the appropriate antibody and 20µl of Magnetic protein A/G beads (Magnachip, EMD-Millipore, Billerica, MA, USA) were used for the IP step ...
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bioRxiv - Microbiology 2023Quote: For top-down EF-P measurements the purified proteins were desalted on the ZipTip with C4 resin (Millipore, ZTC04S096) and eluted with 50 % (v/v ...
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bioRxiv - Molecular Biology 2023Quote: DRIP-WB assay was performed using Magna Nuclear RIPTM (Native) RNA-Binding Protein Immunoprecipitation Kit (Millipore/Sigma # 17-10522) following the manufacturer’s recommended procedure with modifications ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein was eluted with 4 CVs of imidazole-free wash buffer supplemented with 150 µg/mL 3xFLAG peptide (Sigma). This was analysed by SDS-PAGE before being concentrated ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein complex was eluted with 500 μL elution buffer (0.4 mg/ml 3X Flag peptide (Millipore Sigma F4799) in wash buffer ...
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bioRxiv - Biophysics 2023Quote: ... Lysis was done either by resuspending the cell pellet in 0.8mL of the detergent-based BugBuster Protein Extraction Reagent (Millipore) and agitating for 20min on a rotator ...
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bioRxiv - Biochemistry 2023Quote: ... The dialyzed protein solution was then concentrated using Amicon Ultra-4 Centrifugal Filter Units 30 K (Millipore, MA #UFC803024) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... 30–50 μg of protein was fractionated by 10% SDS–PAGE and blotted onto an Immobilon PVDF membrane (Millipore). Non-specific binding sites were blocked by incubation with 5% BSA in Tris-buffered saline (TBS ...
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bioRxiv - Cell Biology 2023Quote: ... Cytokine analysis was performed by Cincinnati Children’s Hospital Research Flow Cytometry Core per kit instructions (Milliplex MAP human protein panel, Millipore-Sigma).
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bioRxiv - Molecular Biology 2023Quote: The following commercially available antibodies were used to detect targeted proteins: anti-RBM17 (1:1500 dilution; HPA037478, Sigma-Aldrich), anti-SF3B1 (1:3000 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... equal amounts of protein per sample were subjected to SDS–PAGE and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore). For immunoprecipitation of FLAG-tagged proteins ...
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bioRxiv - Immunology 2023Quote: ... Proteins were subsequently transferred onto nitrocellulose membranes and immunoblot analysis was performed with following antibodies: HA (Sigma-Aldrich, H6908), FLAG (M2 ...
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bioRxiv - Microbiology 2023Quote: ... The gel was first washed with water and then incubated with c.a 10 mL of InstantBlueTM Ultrafast Protein Stain (Sigma) for at least 1 hour.
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bioRxiv - Cell Biology 2023Quote: ... Fractions containing the protein of interest were pooled and concentrated using centrifugal filter units (Amicon, 10KDa cut-off; Millipore, Ultra-4 UFC801024 and/or Ultra-0.5 UFC501024) ...
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bioRxiv - Cancer Biology 2023Quote: ... was purified as described (Noland et al., 2016) with the exception that the proteins were treated with 2.5% hydroxylamine (Sigma) at pH 7.0 for one hour at room temperature prior to size exclusion chromatography ...
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bioRxiv - Plant Biology 2023Quote: ... Frozen tissue was homogenized with 2 volumes of protein extraction buffer (150 mM NaCl, 50 mM Tris [pH 7.5], 0.1% Nonidet P-40 [Sigma-Aldrich] ...