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Citations for Millipore Sigma :
6101 - 6150 of 7633 citations for Yellow Fever Virus NS1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Protein expression was stably knocked down in Jurkat T cells by RNA interference using Mission shRNA plasmids (Sigma-Aldrich). Lentiviral particles were generated by transfecting HEK293T cells with pMD2G ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted proteins were buffer exchanged into 1x PBS (pH8.0) using Amicon Ultra-15 Centrifugal Filter Unit (10 kDa) (Millipore) and flash-frozen.
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bioRxiv - Plant Biology 2023Quote: ... alkylation and tryptic digestion (50 µg of protein per sample) was performed in centrifugal filters (Amicon Ultra-0.5, 30 kDa cut-off, Merck Millipore) according to the FASP protocol (Wiśniewski et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... Excess reagents were removed by MeOH/CHCl3 protein precipitation or concentration-dilution using a 0.5 mL Centrifugal Filter (3K, Millipore). Pellets were washed by 500 μL MeOH/H2O (9:1 ...
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bioRxiv - Plant Biology 2024Quote: ... resuspended in 1 mL total protein extraction buffer (10 mM Phosphate Buffered Saline (PBS) at pH 7.4 (Sigma P5368), 0.5% v/v NP-40 (Nonidet™ P 40 Substitute from Sigma 74385) ...
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bioRxiv - Plant Biology 2024Quote: ... His- and GST-tagged proteins were detected with anti-His (Monoclonal Anti-polyHistidine antibody, Sigma H1029, 1:5000 dilution) or anti-GST (Mouse anti-GST monoclonal antibody ...
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bioRxiv - Biochemistry 2024Quote: ... The purified protein was concentrated to 0.6 mL using a 10 kD cut-off Amicon Ultra centrifugal filter (Millipore). The sample was then loaded into a Superdex 200 Increase 10/300 GL column (Cytiva ...
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bioRxiv - Molecular Biology 2023Quote: DRIP-WB assay was performed using Magna Nuclear RIPTM (Native) RNA-Binding Protein Immunoprecipitation Kit (Millipore/Sigma # 17-10522) following the manufacturer’s recommended procedure with modifications ...
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bioRxiv - Plant Biology 2024Quote: ... The proteins were separated by 7.5% SDS-PAGE and transferred to an Immobilon-P membrane (Millipore, Billerica, MA, USA), as previously described (Towbin et al. ...
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bioRxiv - Cell Biology 2024Quote: 500 μg of crude cell lysate was precleared with 10 μl of PureProteome Protein A Magnetic Beads (Merck Millipore, Burlington ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Protein lysates from the adipose tissue and serum samples were analyzed for inflammatory markers (Millipore Sigma #MCYTOMAG-70K-PMX) and adipokines (Millipore Sigma #MADMAG-71K-07 ...
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bioRxiv - Cell Biology 2024Quote: ... anti-N protein (1:1000, CiteAb #40588-T62) and mouse anti-acetylated α-tubulin (1:200, Sigma-Aldrich #T7451). The secondary antibodies used were goat anti-mouse and goat anti-rabbit conjugated to Alexa Fluor 568 or 488 (1:500 ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were concentrated by filtration of the eluate in 15-mL Amicon® Ultra 50k filters (Millipore, Burlington, MA). Subsequently ...
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bioRxiv - Biochemistry 2024Quote: Human C-reactive protein (Cat. No. C4063) and Human erythrocyte catalase (Cat. No. C3556) were obtained from Sigma Aldrich chemicals ...
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bioRxiv - Biophysics 2024Quote: ... Each protein was dialyzed and concentrated against 100LmM ammonium acetate (pH 7.4) using an Amicon 0.5-mL microconcentrator (EMD Millipore) with a 10-kDa MW cutoff and stored at –80L°C until used ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mg mL-1 catalase from bovine liver (lyophilized powder, 2000-5000 units/mg protein, Sigma-Aldrich, Missouri, US) and 50 mM SPB (pH 7.2 ...
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bioRxiv - Neuroscience 2024Quote: Equal amounts of proteins from total cell lysates were resolved by SDS-PAGE and transferred to nitrocellulose membranes (Millipore). The blots were blocked with 5% w/v BSA and 0.05% NaN3 in TBST and incubated with primary antibodies at 4°C overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... Detection of specific protein bands was accomplished using a rabbit monoclonal anti-GLRX3 antibody (1:1,000, HPA028941, Sigma-Aldrich), ferritin (1:1,000 ...
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bioRxiv - Biophysics 2024Quote: ... The target protein was eluted by application of 5 CVs of buffer E containing 2.5mM d -desthiobiotin (Sigma-Aldrich). Elutions were pooled ...
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bioRxiv - Microbiology 2024Quote: ... and FLAG-tagged proteins were detected by alkaline-phosphatase-conjugated anti-FLAG IgG (A9469, Sigma Aldrich, St. Louis, MO) and 1-Step NBT/BCIP substrate solution (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... before clarification by centrifugation and enrichment for FLAG-tagged proteins using anti-FLAG® M2 Affinity Gel (A2220, Sigma), with elution using 3xFLAG peptide (F4799 ...
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bioRxiv - Biophysics 2024Quote: ... 50 µM mutant protein solution was mixed with excess amount of Atto dye in respective binding buffer (Sigma Aldrich) and incubated overnight at 4°C in a dark place ...
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bioRxiv - Plant Biology 2024Quote: ... Tissue was ground in ice-cold protein extraction buffer (150 mM NaCl, 50 mM Tris-HCl [pH 7.5], 0.1% Nonidet P-40 [Sigma-Aldrich] ...
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bioRxiv - Biophysics 2024Quote: ... Refolded proteins were further purified by size-exclusion chromatography using a Hiload 16/600 Superdex 75 column (Sigma-Aldrich) in buffer A.
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bioRxiv - Neuroscience 2024Quote: ... The resolved proteins were transferred from the gel to Immobilon®-P PVDF membranes (Millipore Sigma, Cat# IPVH00010, USA) in 10x Tris/Glycine buffer (BioRad ...
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bioRxiv - Biochemistry 2024Quote: ... and the protein was filtered through polyvinylidene fluoride ultra-free membrane filter with a 0.22 μm pore size (Millipore). The concentrated and filtered sample was then finally passed through a HiLoad 10/300 Superdex 75 column (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were detected after incubating with peroxidase conjugated secondary antibodies for 1 hour and 5min with ImmobilonR Western (Millipore) after 5x washes ...
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bioRxiv - Biochemistry 2024Quote: ... Fractions containing the expressed protein were combined and concentrated to 2.5 mL with a 30-kDa cut-off centrifugal filter (Millipore), run through the desalting column again ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was eluted with GFB containing 2.5 mM desthiobiotin and concentrated in a 10 kDa MWCO concentrator (Millipore Sigma). Concentrated protein was loaded onto the Superdex 75 Increase 10/300 GL (Cytiva ...
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bioRxiv - Biophysics 2024Quote: ... Proteins were incubated at 50 µM in the presence of 10x excess of Bromo-1,1,1-trifluoroacetate (BTFA, Sigma Aldrich) overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins from the purified PB or pre-sorting fractions were dissolved in a 8 M urea solution (Sigma-Aldrich) before trypsin digestion ...
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bioRxiv - Microbiology 2024Quote: ... Eluted proteins were concentrated to 2.5 mg mL-1 using 100 kDa molecular weight cut-off centrifugal filters (Merck Millipore). To each 200-mesh holey carbon grid (Au R1.2/1.3 ...
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bioRxiv - Microbiology 2024Quote: ... CHIKV nsP1 protein was concentrated to the required concentration with Amicon centrifugal filters (10,000 MWCO Millipore, Burlington, MA, USA), flash-frozen in liquid nitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... The fractions that contained the SsCBE2-C2 protein were collected and concentrated through an Amicon Ultra centrifugal filter (Millipore). Following that ...
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bioRxiv - Neuroscience 2024Quote: ... The eluted protein and the beads were separated by briefly spinning in a SpinX column tubes (Sigma CLS8162-24EA). The purity of purified protein was by running 5 µg of protein on an SDS PAGE gel and performing Comassie stain ...
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bioRxiv - Plant Biology 2019Quote: ... The purified protein was concentrated using an Amicon Ultra centrifugal filter with a molecular mass cut-off (MWCO) of 30 kDa (Millipore) and analysed on a Superdex 200 size-exclusion chromatography (SEC ...
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bioRxiv - Synthetic Biology 2021Quote: ... purified Sx-Cm-MOMP and Sx-CtE-MOMP proteins were spotted directly onto nitrocellulose membranes and incubated for 5 min before being blocked with 5% BSA (Sigma) and probing with the same primary antibodies ...
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bioRxiv - Plant Biology 2021Quote: ... We prepared InsP7 by using 100ng of Mouse IP6K1 (mIP6K1) recombinant protein along with 100 µM of InsP6 (Sigma, USA) in a buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Microbiology 2020Quote: ... The initial rates of reaction were normalised to total protein content as determined using the QuantiPro BCA Assay Kit (Sigma). Control reactions without any substrate (G3P for GapA and GapN ...
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bioRxiv - Molecular Biology 2020Quote: ... 50 μl of Protein G magnetic beads were used and 10 μg of mouse monoclonal anti-FLAG primary antibody M2 (Sigma) were added before overnight incubation at 4°C with the lysate ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitations were performed on 2 mg of total protein by incubating 2h at 4°C with anti-Flag agarose beads (Sigma). Beads were then washed in lysis buffer and bound polypeptides analyzed by SDS-PAGE and immunoblotting ...
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bioRxiv - Cell Biology 2020Quote: ... and 2 mg of protein per lysate were subjected to anti-FLAG immunoprecipitation using 15 ul of packed magnetic anti-FLAG beads (M2, Sigma) for 4h ...
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bioRxiv - Cell Biology 2020Quote: ... Chromatin was diluted 10 times and incubated overnight at 4 °C with 20 µL Protein A/G MagnaChIP beads (Millipore) and 8 µg anti-H3K27me3 (Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... The GST-His6-tagged forms of LC3B-His12 and GATE-16-His12 proteins were expressed in Escherichia coli BL21(DE3) cells (Novagen) harboring the pET-41-based vectors constructed in Lysogeny Broth (LB ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were cleared from cell debris by centrifugation prior to adjustment of protein concentrations between the samples and overnight immunoprecipitation at 4° C with pre-equilibrated anti-HA-agarose (Sigma) or anti-c-myc-agarose (Thermo fisher) ...
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bioRxiv - Immunology 2021Quote: ... 0.1% SDS) and precleared with overheard rotation at 4 degrees for two hours using Protein A+G conjugated magnetic beads (Millipore). Chromatin was incubated with primary antibody against mouse p53 (CM5 ...
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bioRxiv - Cell Biology 2020Quote: ... cell lysates (750 μg total protein) were precleared with empty agarose beads and then incubated with anti-FLAG-M2 agarose affinity beads (Sigma) with rotation overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Equal amounts of protein lysate were resolved by SDS-PAGE and subsequently transferred onto polyvinylidene difluoride Immobilon-FL membrane (Millipore). Following transfer ...
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bioRxiv - Microbiology 2021Quote: ... The primary immunization was carried out using 450 μg of GST cleaved PfCD protein and an equal volume of Freund’s complete adjuvant (F5881 # Sigma-Aldrich) via the intraperitoneal route ...
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bioRxiv - Molecular Biology 2020Quote: ... 20 μg of protein was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (Millipore, Billerica, MA, USA) and transferred onto a polyvinylidene fluoride membrane (Millipore) ...