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Citations for Millipore Sigma :
5651 - 5700 of 7633 citations for Yellow Fever Virus NS1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... After the protein was collected the following day and concentrated using 10 kDa MWCO Amicon Ultra (Merck Millipore). The protein concentration was monitored periodically during the process using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... Expression of the eGFP protein and the PAK1 mutant was induced by doxycycline (2 μg/mL, Sigma-Aldrich, St ...
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bioRxiv - Neuroscience 2023Quote: ... CopiaGag capsid protein was concentrated using a 100kDa MWCO Amicon® Ultra-15 Centrifugal Filter Unit(EMD Millipore) to a final concentration of 0.8 mg/ml ...
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bioRxiv - Biochemistry 2023Quote: ... The pellet was resuspended in 5 mL per gram of cells of a hypotonic solution (5 mM MgCl2, 0.25 mg/mL lysozyme from chicken egg white >40,000 units/mg protein (Sigma) and 1 mM phenylmethylsulfonyl fluoride) ...
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bioRxiv - Cancer Biology 2023Quote: ... The nuclear protein lysate then was pre-cleared by adding 2 μL of poly D[IC] (Sigma-Aldrich) 25 µg/µL and 20 μL of High Capacity Strepavidin agarose beads (Thermofisher #20357) ...
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bioRxiv - Biochemistry 2023Quote: ... GST-tagged protein was eluted off the beads by using 20 mM glutathione solution (Sigma-Aldrich, #G4251-5G). Fractions were analyzed by resolving on 4-20 % gradient SDS-PAGE (BioRAD #4561094 or #4651093) ...
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bioRxiv - Neuroscience 2023Quote: Total protein from BFP expressing iNeurons was extracted using CelLytic™ M Cell Lysis Reagent (Sigma-Aldrich, #C2978) with PhosSTOP™ (Roche ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Total protein was quantified following the method described by Bradford using bovine serum albumin (BSA, Sigma cat. 7906) as standard ...
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bioRxiv - Cell Biology 2023Quote: ... Immunoprecipitation of Flag-tagged proteins was performed with Anti-Flag M2 Affinity Gel (Sigma-Aldrich, cat.no. A2220-1ML) at 4 °C with overhead rotation for 6 h ...
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bioRxiv - Cell Biology 2023Quote: ... The pure proteins were concentrated with an Amicon Ultra-15 centrifugal filter (10 kDa MWCO, Merk Millipore Inc.) to around 20 mg/mL.
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bioRxiv - Biophysics 2023Quote: ... The purified protein (in 25 mM piperazine-N,N′-bis(2-ethanesulfonic acid) (PIPES, P-1851, Sigma-Aldrich) pH 6.8 ...
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bioRxiv - Cell Biology 2023Quote: ... 3 mg of proteins were subsequently incubated for 1h with 10 μl of FAM134B or FAM134C antibodies (Sigma Prestige-HPA012077 and -HPA016492 ...
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bioRxiv - Biophysics 2023Quote: Plasmids for analyte proteins were constructed with gBlocks (Integrated DNA Technologies) inserted into the pET-49b(+) plasmid (Novagen), with a dihydrofolate reductase domain ...
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bioRxiv - Plant Biology 2024Quote: ... GST-fusion proteins were purified by loading total bacterial lysate on a preequilibrated glutathione Sepharose column (Sigma, Germany) using fast performance liquid chromatography system (Cytiva ...
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bioRxiv - Plant Biology 2024Quote: ... 0.5 mg TTN5 protein was combined with 1 U of agarose bead-coupled alkaline phosphatase (Sigma Aldrich, Germany) for degradation of bound GDP to GMP and Pi in the presence of 1.5-fold molar excess of non-hydrolyzable GTP analog GppCp (Jena Bioscience ...
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bioRxiv - Physiology 2024Quote: ... Total protein extracts were obtained by transferring the bronchioids to Dulbecco′s phosphate-buffered saline (DPBS, Sigma-Aldrich) for 30 minutes at 37 °C with gentle agitation to dissolve the alginate ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... expression of the S protein was induced by the addition of 50 ng/ml dox (Sigma-Aldrich, D9891) for 24 h ...
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bioRxiv - Cell Biology 2024Quote: ... Protein was then transferred to a methanol soaked polyvinylidene difluoride (PVDF) membrane (Millipore Immobilon FL 0.45 µm #IPFL00010). The membrane was then PBS washed ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 mM β-mercaptoethanol prior to application of the protein to His-Select Cobalt affinity gel (Sigma-Aldrich). The slurry was rotated for 60 min prior to application to a gravity flow column ...
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bioRxiv - Cell Biology 2024Quote: ... the 6×His tag on the proteins was further cleaved with Thrombin CleanCleave kit (Sigma-Aldrich, Cat# RECMT) overnight at 4 °C on the rocking table after desaltig in 50 mM Tris-HCl ...
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bioRxiv - Microbiology 2024Quote: The culture supernatants containing the secreted proteins were centrifugated (1 h, 30,000xg, 10 °C) and sterilized by filtration (0.22 µm; Express Plus; Merck Millipore). The supernatants were equilibrated to pH 7 with 0.5 mM NaOH and the proteins were purified using two ion exchange chromatographies (Fig ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were eluted from the beads by adding 25 μL of Laemli Sample Buffer 2X (Sigma-Aldrich, #S3401), before heating tubes at 95°C ...
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bioRxiv - Cancer Biology 2024Quote: ... 10-50 μg total protein was fractionated by SDS-PAGE and transferred to PVDF or nitrocellulose membranes (Millipore). Blocking was achieved with 5% milk in Tris-buffered saline with 0.1% Tween-20 (TBST ...
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bioRxiv - Biophysics 2024Quote: ... Oligomeric α-Syn species were removed by filtering the protein through a 50-kD cutoff column (Amicon, Millipore). Aliquots of the protein were prepared ...
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bioRxiv - Biochemistry 2024Quote: ... The cleaved protein was collected and concentrated using 100 kDa MW cut-off spin concentrators (Amicon Merck-Millipore) separated by size-exclusion chromatography (SEC) ...
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bioRxiv - Biochemistry 2024Quote: ... The eluted protein fractions were pooled and concentrated using a 10 kDa cut-off (Amicon filter, Millipore, USA). After affinity purification ...
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bioRxiv - Microbiology 2024Quote: ... Samples normalized for the protein content were separated by SDS-PAGE and transferred onto a PVDF membrane (Millipore). After washing with TBS buffer twice ...
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bioRxiv - Cancer Biology 2024Quote: ... Proliferating AT2 cells were identified by immunofluorescence for the AT2 marker surfactant protein-C (SPC, EMD Millipore, #AB3786) and proliferation maker Ki67 (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... Total protein extracts were fractioned on SDS-PAGE and examined by Western blot with mouse anti-myc (Sigma), mouse anti-Pgk1 (Thermofisher) ...
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bioRxiv - Microbiology 2024Quote: Fifteen amino acid (aa) peptides overlapping 12 aa spanning the structural proteins of CHIKV were purchased from Sigma and were divided into 4 overlapping peptide pools ...
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bioRxiv - Immunology 2024Quote: ... Protein was eluted from Sepharose A by addition of 35 μL sample loading buffer (Sigma-Aldrich, S3401-10) and incubation at 80 °C for 5 min ...
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bioRxiv - Microbiology 2024Quote: ... samples were thawed and 10 µl of total protein extract was added to 100 µM nitrocefin (Sigma-Aldrich) solution in PIPES 2 mM pH 6.8 and substrate hydrolysis measured spectrophotometrically over 30 minutes (Cytation5 ...
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bioRxiv - Molecular Biology 2024Quote: ... we used commercially available ELISA kits: hs-CRP Human C-Reactive Protein ELISA Kit (#RAB0096, Sigma-Aldrich, Germany) and Mercodia Insulin ELISA (#10-1113-01 ...
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bioRxiv - Plant Biology 2024Quote: ... Protein concentrations of the cell lysate or EV pellets were quantified with Pierce 660 nm reagent (Sigma #22660) using the manufacturer’s protocol and comparing to a bovine serum albumin standard curve.
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bioRxiv - Genetics 2024Quote: ... complex was prepared 15 min before transfection by mixing gRNAs (1.5 µM) with Cas9 protein (0.45 µM) (Sigma, Catalog no ...
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bioRxiv - Immunology 2024Quote: ... that contained protein: carbohydrate: fat (20:20:60, kcal%) plus high fructose (23.1 g/L; F3510, Sigma,USA) and glucose (18.9 g/L ...
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bioRxiv - Cell Biology 2024Quote: ... 10-30 μg of protein was resolved by electrophoresis and then transferred to a polyvinylidene difluoride membrane (Millipore). Membranes were blocked in TRIS-buffered saline with 0.1% tween 20 and 5% BSA for 1 hour and then incubated overnight with primary antibody at a dilution of 1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of total protein were loaded onto an SDS-PAGE before transfer onto a nitrocellulose membrane (Millipore). The membranes were blocked in 5% (w/v ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Fractions containing the protein were pooled and concentrated with a 30 kDa cutoff Amicon centrifugal filter (Sigma UFC9030) and loaded onto a Superdex 200 size exclusion column equilibrated with the storage buffer (25mM HEPES pH 7.4 ...
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bioRxiv - Genetics 2024Quote: ... cell surface protein samples were concentrated into 0.1 ml volumes using a 30 kDa centrifugal concentrator (Merck Millipore).
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bioRxiv - Molecular Biology 2024Quote: ... For dTAG-13 induced protein degradation cells were treated with 500 nM dTAG-13 (Sigma-Aldrich, SML2601-5MG) or an equivalent volume of DMSO (Sigma-Aldrich ...
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bioRxiv - Pathology 2024Quote: ... 0.5% Triton and lysates were incubated with rabbit or mouse isotypic control antibodies and G-protein beads (Sigma) for 1 hr at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Collected cells were immediately spun down and resuspended in BugBuster® Protein Extraction reagent (Novagen, Merck and Co. Inc) to lyse the bacterial cells and release proteins ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were separated by 10% SDS-PAGE gel and transferred to 0.45 µm polyvinylidene difluoride (PVDF) membrane (Millipore, USA). After blocking by with 5% non-fat dry milk for 2h at room temperature ...
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bioRxiv - Genetics 2020Quote: ... Approximately 5 mg of extract containing the corresponding recombinant protein was incubated with 25 µl of glutathione sepharose (SIGMA) and subsequently mixed with yeast extracts expressing Sen1 TAP-Nter (typically 0.5 mg of extract per binding reaction) ...
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bioRxiv - Molecular Biology 2019Quote: ... and 3×FLAG::GFP-tagged protein and associated RNAs were eluted with 100 μg/mL 3×FLAG peptide (Sigma). The eluates were incubated with TRIzol reagent (Invitrogen) ...
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bioRxiv - Molecular Biology 2020Quote: ... 25 μL of magnetic Protein G Dynabeads were coated with 2 μg of M2 anti-FLAG antibody (F1804, Sigma) for 1 hr at 4°C ...
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bioRxiv - Cell Biology 2020Quote: Recombinant GST-mm-FMN2-eFSI (GST-FMN2-eFSI) proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
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bioRxiv - Cell Biology 2020Quote: Proteins were separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride membranes (Millipore). After in-cubated with antibodies ...
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bioRxiv - Cell Biology 2019Quote: Protein was extracted from isolated cell pellets or whole liver tissue using RIPA buffer with protease inhibitors (Sigma-Aldrich). Equal amounts of protein were loaded and separated by SDS-PAGE gel electrophoresis on 4%-20% Criterion gels (BioRad ...