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Citations for Millipore Sigma :
5701 - 5739 of 5739 citations for Heme Assay Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: RNA was prepared for Iso-Seq using the Sigma Spectrum Plant Total RNA Kit including On-Column DNAse I Digestion (both products Sigma-Aldrich, St. Louis, MO, USA). One Hen17-A07 plant was sectioned into three parts (roots ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... ground and homogenized western corn rootworm larval tissue (3-7 larvae per biological replicate, n=8-11) using the GenElute Universal Total RNA Purification Kit (Sigma-Aldrich, St. Louis, MO, USA). A NanoDrop spectrophotometer (ND-1000 ...
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bioRxiv - Neuroscience 2021Quote: Genomic DNA was extracted from tail tissues and amplified with specific PCR primers using REDExtract-N-Amp™ Tissue PCR kit (Cat. No. XNAT-100RXN, Sigma-Aldrich, Saint Louis, MO) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue digestion for DNA extraction and subsequent polymerase chain reaction (PCR) was performed with the prepared sample buffer from REDextract-N-Amp PCR KIT (Sigma-Aldrich, St. Louis, United States) or DreamTaq™ Hot Start Green DNA Polymerase (Thermo Fischer Scientific ...
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bioRxiv - Synthetic Biology 2022Quote: Genomic DNA of the evolved mutants was isolated from LB overnight cultures using the GenElute™ Bacterial Genomic DNA Kit (Sigma-Aldrich St. Louis, MO). The extracted DNA was evaluated by gel electrophoresis and quantified by a NanoDrop spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2020Quote: ... Quantitative Real-time PCR reactions were performed on a Bio-Rad CFX96 Termo-cycler with Kapa Syber Fast qPCR master mix (2x) kit (Sigma-Aldrich, Saint Louis, MO, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: The PLA assay was performed to determine the in situ binding between LC3 and ATG9a or ATG9b by using the Duolink® In Situ Red Starter Kit purchased from Sigma Aldrich (catalog number DUO92101), following the protocol provided by the manufacturer.
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bioRxiv - Genomics 2020Quote: ... Supernatant was aspirated before the isolation of single nuclei was performed using the Nuclei PURE Prep nuclei isolation kit (Sigma-Aldrich, St. Louis, MO, USA) in conjunction with a modified protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: Total RNA of the collected/dissected petals (see supplementary fig. S2) was extracted using Spectrum Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA). cDNA was synthesized following the methods described in Supporting Information Methods S4 in Lin and Rausher (2021) ...
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bioRxiv - Microbiology 2021Quote: RNA extract from a single female adult tick was converted to cDNA using the Complete Whole Transcriptome Amplification Kit (WTA2, Sigma-Aldrich, Saint Louis, MO, USA). Purification of cDNA was done using the MSB Spin PCRapace kit (Stratec Molecular ...
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bioRxiv - Microbiology 2020Quote: ... All transformants were screened by PCR using gene-specific primers (Table S2, Fig. S2c) and were verified by Southern hybridization analyses (DIG DNA Labeling and Detection Kit, Sigma-Aldrich, St. Louis, MO, USA) (Fig ...
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bioRxiv - Immunology 2021Quote: ... Vero E6 monolayers are washed and incubated for 5-6 days at 37 degrees after which plates are scored using the vitality marker WST-8 (colorimetric cell counting kit, Sigma Aldrich, cat 96992-3000TESTS-F). To this end ...
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bioRxiv - Bioengineering 2020Quote: Plasmid DNA was purified after overnight culture in standard lysogeny broth containing 100 µg/mL ampicillin using a miniprep plasmid DNA isolation kit (Sigma Aldrich, St Louis, MO; PLN350) and quantified using spectrophotometry (NanoDrop One ...
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bioRxiv - Plant Biology 2023Quote: The total RNA was extracted from PEG-treated (24 h) and untreated control (0 h) samples using Spectrum plant total RNA Kit (Sigma Aldrich, St. Louis, MO, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 mg of leaf powder was used for RNA extraction following protocol A of the Spectrum™ Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO). RNA was eluted in 50 μL elution buffer ...
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bioRxiv - Plant Biology 2022Quote: Harvested tissue was grounded to a fine powder in liquid nitrogen and RNA was extracted using the Spectrum Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA) following the manufacturer’s recommendations ...
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bioRxiv - Genetics 2024Quote: ... quantification in the heads of both starved and non-starved miR-210 KO and WT flies was carried out using the Triglyceride Quantification Kit (Sigma-Aldrich, St. Louis, Missouri, USA), following the provided manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: RNA was extracted from 100 mg of frozen ground leaves per replicate using the Spectrum Plant Total RNA kit (Sigma-Aldrich, St Louis, MO, USA) followed by treatment with RNase-free DNase using the On-Column DNase I Digestion Set (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from 100 mg of frozen and ground samples using the Spectrum™ Plant Total RNA Kit (Sigma, St. Louis, Missouri, USA) according to the manufacturer’s Protocol A ...
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bioRxiv - Bioengineering 2023Quote: ... the first step was to remove the iodixanol through dialysis using Pur-A-LyzerTM Mini 6000 dialysis kit (PURN60030, Sigma-Aldrich, Co., STL, MO, USA). Then SEC-DGUC-1 was subjected to in-solution digestion prior to fractionation on an Xbridge™ C18 column (4.6 × 250 mm ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were isolated from snap-frozen frontal cortex (anterior to the anterior forceps of the corpus callosum) samples from one hemisphere using Nuclei Isolation Kit: Nuclei EZ Prep (Sigma-Aldrich Cat. No. NUC-101). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was extracted from frozen samples in the manual of the GenElute Bacterial DNA Isolation kit (Sigma Aldrich, NA2110-1KT, St. Louis, MO, USA) with the following minor modifications as previously described60 ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was extracted from 21 days-after-flowering (DAF) sunflower seeds using the Spectrum Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: Tissues were ground into a fine powder in liquid nitrogen and total RNA was extracted using the Spectrum™ Plant Total RNA kit (Sigma-Aldrich, St. Louis, MO). Sequencing libraries were produced using the TruSeq RNA Sample Preparation kit v2 (Illumina ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was extracted from a tail biopsy and genotypes were determined using the Extract-N-Amp Tissue PCR kit (Sigma-Aldrich, St Louis, MO, USA). The following primer sequences (Integrated DNA Technologies ...
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bioRxiv - Developmental Biology 2024Quote: ... To detect Edu signal was used “EdU IV Imaging Kit 647 S” based on the Click-it reaction with azides conjugated to fluorochromes (Sigma Aldrich, BCK647-IV-IM-S) following instructions of the manufacturer.
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bioRxiv - Evolutionary Biology 2024Quote: ... we grinded the plant tissue into a fine powder with a pestle and a mortar in the presence of liquid nitrogen and processed the samples using the Spectrum™ Plant Total RNA-Kit from Sigma-Aldrich (product number: STRN250), including on-column DNA digestion using the on-column DNAse kit (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2024Quote: ... RNA from leaf and tuber tissue collected at each time point was done using the Spectrum Plant Total RNA Extraction kit (Sigma-Aldrich, St. Louis, MO, USA). The RNA was treated with Turbo DNA-free kit ...
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bioRxiv - Immunology 2021Quote: Cytotoxicity was assessed via lactate dehydrogenase (LDH) concentrations measured in 100 ul of basolateral medium incubated with the reaction mixture of a cytotoxicity detection kit (Sigma-Aldrich, Roche, Saint Louis, MO, USA) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: Total RNA was extracted from leaf tissue collected at each time point for all species using the Spectrum Plant Total RNA Kit (Sigma-Aldrich Co., St. Louis, MO, USA) following Protocol A ...
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bioRxiv - Biochemistry 2023Quote: Invitrogen™ eBioscience™ Annexin V Apoptosis Detection Kit FITC (Cat. No. 88-8005-72) was used in combination with Propidium Iodide Solution (Sigma-Aldrich, Cat. No. P-4864-10mL) as described in manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Cytokine levels in matched BAL fluid and plasma collected from patients were measured using the multiplexed human cytokine/chemokine magnetic bead kits from Millipore (HCYTMAG-60K-PX41 and HCYP2MAG-62K) according to manufacturer’s protocol (HCYTOMAG-60K Rev ...
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bioRxiv - Neuroscience 2022Quote: ... and TNF-α were measured simultaneously using the multiplexed kit “Mouse High Sensitivity T Cell Magnetic Bead Panel” (MHSTCMAG-70K, MILLIPLEX, EMD Millipore Corp., Billerica, MA, USA). The assay was performed according to the manufacturer’s instructions with an overnight incubation step (17 hours ...
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Spray-induced gene silencing (SIGS) as a tool for the management of Pine Pitch Canker forest diseasebioRxiv - Plant Biology 2024Quote: ... three samples consisting of both treated and mock seedlings were collected and used for total RNA extraction with the Sigma mirPremier® microRNA Isolation Kit (Sigma Aldrich, St. Louis, MO, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... A 1.5 mL of overnight culture was used for DNA extraction using the GenElute™ Bacterial Genomic DNA Kit (Sigma-Aldrich, St. Louis, MI, United States) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: Senescence was verified by staining for senescence-associated-β-galactosidase (SA-β-gal) using a commercially available SA-β-gal staining kit (Sigma, catalogue no. CS0030). Following manufacture instructions ...
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bioRxiv - Immunology 2022Quote: ... pH 8.0 to a final concentration of 0.2nM and PCR amplified for cloning into T7 phage vector arms (Novagen/EMD Millipore Inc. T7 Select 10-3 Cloning kit). Detailed protocol can be found in dx.doi.org/10.17504/protocols.io.j8nlkkrr5l5r/v1 ...
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bioRxiv - Cell Biology 2023Quote: ... ∼1 kb 5’ homology arm and ∼1 kb 3’ homology arm of msps genomic region were amplified from the genomic DNA (Sigma, Extract-N-Amp™ Tissue PCR Kit), mutated to be insensitive to gRNAs ...
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bioRxiv - Molecular Biology 2024Quote: ... The presence/absence of receptor–receptor molecular interactions in the samples was detected using the Duolink II in Situ PLA Detection Kit (developed by Olink Bioscience, Uppsala, Sweden; and now distributed by Sigma-Aldrich as Duolink®, using PLA® Technology).