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Citations for Millipore Sigma :
5501 - 5550 of 5739 citations for Heme Assay Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: For purification the Amicon® Pro-Affinity Concentration Kit Protein A with 100 kDa Amicon® Ultra-0.5 Device (Merck Millipore, UK) as used as described by the manufacturer ...
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bioRxiv - Cell Biology 2022Quote: The determination of telomerase enzyme activity was done in HEKn cells by using TeloTAGGG Telomerase PCR Plus Kit (Sigma Aldrich, USA, 12013789001) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... The genomic DNA of kanamycin-sensitive and chloramphenicol-sensitive clones was isolated using the GenElute™ Bacterial Genomic DNA Kit (Sigma-Aldrich), and analyzed by PCR to confirm the gene replacement using primers pairs TP_ctg3-43_f / TP_ctg3-43_r in reactions (20 μL ...
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bioRxiv - Neuroscience 2020Quote: ... and retinal levels of human Pi and serum levels of human insulin were measured using human Pi and human insulin ELISA kits (EZHPI-15K and EZHI-14K, respectively; Millipore, Darmstadt, Germany) according to the manufacturer’s instructions and as described previously (Isiegas et al. ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from 200 mg of pollen tubes using SpectrumTM Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA). RNA-seq libraries were performed using the non-stranded mRNA Kit from Illumina (San Diego ...
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bioRxiv - Microbiology 2021Quote: ... DNase I treatment was given to avoid genomic DNA contamination followed by purification of total RNA using GenElute™ Total RNA Purification Kit (Sigma-Aldrich). The purified RNA was quantified using a NanoDrop 2000 spectrophotometer (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: HT29-18N2 cells (obtained from ATCC) (RRID:CVCL_5942) were tested for mycoplasma contamination with the Lookout mycoplasma PCR detection kit (Sigma-Aldrich, St. Louis, MO). Mycoplasma negative HT29-18N2 cells were used for the experiments presented here.
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bioRxiv - Neuroscience 2021Quote: ... were measured with a human cytokine/chemokine panel utilizing Luminex 200 xMap technology and the MILLIPLEX MAP ® multiplex kit (Luminex xMAP technology; EMD Millipore, Chicago ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed three more times in TBS-T before antibody detection using enhanced chemiluminescence horseradish peroxidase substrate detection kit (Millipore, Hertfordshire, UK). Imaging was conducted using a G:Box Chemi-XR5 (Syngene ...
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bioRxiv - Developmental Biology 2022Quote: ... Terminal deoxynucleotide transferase (TdT)-mediated dUTP nick end labeling (TUNEL) was performed on frozen retinal cryosections using the ApopTag Fluorescein Direct In Situ Apoptosis Detection Kit (Millipore, Billerica, MA). TUNEL staining was performed according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: The number of apoptotic cells were determined using the In Situ Cell Death Detection Kit (Sigma-Aldrich, 11684795910, St. Louis, MO, USA) in paraffin-embedded tissue sections ...
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bioRxiv - Biochemistry 2022Quote: HEK293 cells were obtained from ATCC and were tested negative for mycoplasma using LookOut® PCR-based kit (MP0035-1KT Sigma-Aldrich). HEK293-Klotho cells stably expressing Klotho were a gift from Dr ...
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bioRxiv - Pathology 2020Quote: ... Eleven markers were simultaneously measured in the samples using a MILLIPLEX Mouse Cytokine/Chemokine 11-plex kit (Millipore, St. Charles, MO, USA) according to the manufacturer’s protocol ...
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bioRxiv - Zoology 2020Quote: ... Cells were allowed to adhere overnight at 26°C prior to being assessed for β-galactosidase activity associated with cellular senescence using a commercially available Senescence Cells Histochemical Staining Kit (Sigma Aldrich). The manufacturer’s protocol was followed with the following modifications ...
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bioRxiv - Genomics 2020Quote: Total RNA was extracted from immature seeds of Trombay wild and TU94-2 plants with the help of Spectrum Plant Total RNA Kit (Sigma-Aldrich, USA) and treated with DNAse-I (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... PDMS with the standard 1:10 weight ratio of crosslinker to base (SYLGARD 184 Silicone Elastomer Kit, Dow Chemical) was diluted with hexane (Sigma-Aldrich, 34859) in a volume ratio of 1:9 ...
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bioRxiv - Microbiology 2021Quote: ... USA) QIAamp 96 DNA QIAcube HT Kit with the following modifications: enzymatic digestion with 50 μg lysozyme (Sigma, St. Louis, MO, USA) and 5U each of lysostaphin and mutanolysin (Sigma ...
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bioRxiv - Pathology 2021Quote: ... Cytokine and chemokine levels in monkey sera were measured using a MILLIPLEX MAP Non-Human Primate Cytokine Magnetic Bead Panel - Premixed 23 Plex - Immunology (Milliplex MAP kit, Merck Millipore, Burlington, MA), which includes the following 23 cytokines and chemokines ...
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bioRxiv - Genomics 2021Quote: ChIP was performed thrice independently for each antibody using 1×106 SK-N-SH cells for each transcription factor using the EZ-Magna ChIP kit (Millipore Sigma, USA), as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Ethanol 70% (vol/vol) was added and the contents were placed in columns (GenElute® mammalian total RNA miniprep kit, Sigma Aldrich). The RNA was extracted according to the manufacturer recommendations ...
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bioRxiv - Physiology 2020Quote: ... TUNEL staining was performed using a protocol modified from the ApopTag peroxidase in situ apoptosis detection kit (Merck-Millipore, Cat no. S7100). Paraffin sections of adipose tissues were prepared as above ...
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bioRxiv - Cancer Biology 2021Quote: ... Aforementioned single clone cultures were STR validated and screened for any mycoplasma contamination using PCR based Myco detection kit (Sigma, MP0035-1KT) before use ...
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bioRxiv - Biochemistry 2021Quote: Reduced and oxidized fractions of nicotinamide adenine dinucleotide phosphates (NADPH/NADP) were measured using the NADP/NADPH Quantitation kit (Sigma-Aldrich, USA) adhering to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... IL-17A and TNFα were quantified in culture supernatants using the mouse-specific Milliplex® multi-analyte panel kit MT17MAG-47K (Millipore; Sigma) and the MagPix® instrument platform with related xPONENT® software (Luminex Corporation) ...
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bioRxiv - Cell Biology 2021Quote: DNA extraction from mouse tail clips obtained at 7-10 days of age was performed using REDExtract-N-Amp™ Tissue PCR Kit (Cat#R4775, Sigma Aldrich) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... Fluorescent membrane labelling of the VE was achieved using the CellVue Claret Far Red Fluorescent Cell Linker Kit (Sigma-Aldrich, MINICLARET-1KT). Briefly ...
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bioRxiv - Cell Biology 2020Quote: HT29-18N2 cells (obtained from ATCC) (RRID:CVCL_5942) were tested for mycoplasma contamination with the Lookout mycoplasma PCR detection kit (Sigma-Aldrich, St. Louis, MO). Mycoplasma negative HT29-18N2 cells were used for the experiments presented here.
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bioRxiv - Genomics 2021Quote: ... These pX330-mC plasmid DNAs and donor DNA plasmid were isolated using FastGene Plasmid Mini kit (Nippon Genetics, Tokyo, Japan) and filtered using MILLEX-GV® 0.22 μm filter unit (Merck Millipore, Darmstadt, Germany) for microinjection ...
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bioRxiv - Immunology 2021Quote: ... Bulk endotoxin-free preparations of VIU-1005 and the empty control plasmid (control) were prepared for animal studies using GenElute™ HP Select Plasmid Gigaprep Kit (Sigma, Germany).
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA isolated from a 5-mL marine broth overnight culture was extracted with the GenElute™ Bacterial Genomic DNA Kit (Sigma-Aldrich) and used as template ...
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bioRxiv - Genomics 2021Quote: ... For the VPA treatment hiPSCs were differentiated to endoderm using a 2-day DE Induction Kit following the manufacturer’s instructions and from day 0 of differentiation 400uM VPA (valproic acid sodium salt 98%, Sigma Cat. # P4543-10G) in DMSO was added to cell cultures.
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bioRxiv - Microbiology 2020Quote: ... Templates used were either bacterial DNA extracted with the GenElute™ Bacterial Genomic DNA Kit or phage DNA extracted from phage lysates that had been precipitated as previously described and then extracted using the GenElute™ Bacterial Genomic DNA Kit with an extended proteinase K incubation of 1 hour (Sigma-Aldrich). 50 ng of template was used for each PCR ...
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bioRxiv - Microbiology 2020Quote: ... All cell cultures and chlamydial stocks were routinely tested for Mycoplasma contamination using the Mycoplasma PCR detection kit (Sigma, St. Louis, MO). Chlamydia trachomatis strain L2/434 Bu was obtained from the laboratory of Dr ...
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bioRxiv - Plant Biology 2022Quote: ... samples were obtained from the tissues indicated in the text and RNA was isolated using the Spectrum™ Plant Total RNA Kit (Sigma-Aldrich). Total RNA (1 μg ...
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bioRxiv - Neuroscience 2023Quote: Cytokines from mouse brain homogenates prepared in section 2.7 were measured using Millipore mouse cytokine multiplex kits (EMD Millipore Corporation, Billerica, MA). Calibration curves from recombinant cytokine standards were prepared with threefold dilution steps in the same matrix as the samples ...
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bioRxiv - Bioengineering 2023Quote: ... ProteoExtract® native membrane protein extraction kit and 10 kDa Amicon® Ultra-15 centrifugal filters were purchased from Millipore Sigma (USA). Assays for measuring cell toxicity were purchased from Promega (Madison ...
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: ... Primary antibodies were diluted in the Antibody Diluent Reagent from the kit [mouse anti-c-myc (clone 9E10; 1:200, Sigma-Aldrich #11667149001) and rabbit anti-HA (1:200 ...
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bioRxiv - Microbiology 2023Quote: ... All cell lines were tested every two weeks for mycoplasma contamination either by in-house DAPI staining or LookOut® Mycoplasma PCR Detection Kit (Sigma-Aldrich).
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bioRxiv - Genomics 2023Quote: ... Filtered nuclei suspension was pelleted as instructed and then resuspended in 50 ul Storage Buffer from the EZ nuclei kit (Sigma Aldrich, NUC101). For downstream processing ...
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bioRxiv - Pathology 2023Quote: The SA-βgal activity was assessed in RPE cells at the end of the experiment using the Senescence Detection Kit (Sigma-Aldrich, #CS0030) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... using either a control normal rabbit IgG or rabbit monoclonal anti-MSI2 antibody and the Magna RIP RNA-binding Protein Immunoprecipitation kit (cat#17-700, Millipore, Burlington, MA). Manufacturer’s instructions were followed with the exception that RNeasy MinElute Cleanup kit (cat#74202 ...
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bioRxiv - Cell Biology 2023Quote: ... and Vap33 and Git in Drosophila larval tissues were detected in situ using the Duolink® In Situ Red Starter Kit Mouse/Rabbit (Sigma, DUO92101) according to the instructions of the manufacturer ...
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bioRxiv - Plant Biology 2023Quote: ... were processed to isolate RNA starting from at least 50 mg of lyophilized tissue using the Spectrum™ Plant Total RNA Kit (Sigma-Aldrich) following manufacturer’s instructions (Nerva et al. ...
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bioRxiv - Plant Biology 2023Quote: ... were grown to late-log phase in photoautotrophic standard conditions for total RNA extractions using the Spectrum Plant Total RNA Kit (Sigma-Aldrich, STRN50) by following manufacturer instructions with the following modifications ...
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bioRxiv - Microbiology 2022Quote: Water samples collected from each site were vacuum filtered using the Millipore All-Glass Filter Holder kit (EMD Millipore Corporation, Billerica, MA). Collected water from each replicate was filtered through Whatman filter membrane to remove coarse to medium debris ...
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bioRxiv - Biochemistry 2024Quote: ... and potential positive clones were grown overnight in LB media for plasmid production and further purification with GenElute™ Plasmid Miniprep Kit (Sigma-Aldrich). After confirmation of the insert sequence by sequencing (DNA Sequencing Unit ...
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bioRxiv - Cancer Biology 2023Quote: ... a polymerase chain reaction (PCR)-based method was used to screen for mycoplasma once a month employing the LookOut® Mycoplasma PCR Detection Kit (MP0035, Sigma-Aldrich) and JumpStart™ Taq DNA Polymerase (D9307 ...
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bioRxiv - Immunology 2024Quote: ... Mice were injected with approximately 107 total IU of lentivirus measured by qPCR Lentivirus Titer kit (abm, #LV900) and delivered by two 10 µL injections using a 25 µL Hamilton syringe (Sigma-Aldrich, #20787) on a stereotaxic alignment system (Kopf ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The soluble and insoluble protein fractions were extracted using a CelLytic™ MEM Protein Extraction Kit (Sigma–Aldrich, St. Louis, MO, USA) and a CelLytic™ NuCLEAR™ Extraction Kit (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2024Quote: ... Plasma concentrations of acylated ghrelin and leptin were determined by commercial ELISA kits according to the manufacturers’ instructions (Spi Bio #A05117.96 and Millipore #EZML-82K, respectively).