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Citations for Millipore Sigma :
5651 - 5700 of 5739 citations for Heme Assay Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... the cultures were washed 3 times in PBS for 5 minutes and incubated with Duolink Plus and Minus PLA probes (diluted 1:5) followed by Duolink In Situ Orange Starter Kit Mouse/Rabbit (Sigma, cat. no. DUO92102), according to the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2024Quote: ... To indicate possible insulin-resistance among obese mice the level of non-esterified fatty acids was measured using an ELISA kit (Sigma-Aldrich, EZRMI-13K). Both assays were conducted according to the attached protocol ...
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bioRxiv - Microbiology 2024Quote: ... the total DNA from KVJ3 and KVJ2 cultures were isolated using a DNA Isolation Kit for Cells and Tissues (Sigma-Aldrich, Taufkirchen, Germany). Universal primers 27F and 1492R were used in the amplification and Sanger sequencing of single bacterial isolates at LGC Genomics GmbH ...
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bioRxiv - Microbiology 2024Quote: ... The HEp-2 and McCoy cells were verified to be mycoplasma negative using the LookOut Mycoplasma PCR Detection kit (Sigma, St. Louis, MO). C ...
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bioRxiv - Neuroscience 2024Quote: ... lung and serum samples were prepared for cytokine analysis in 96-well plates using Milliplex Map kit (Millipore, Burlington, MA; cat #MCYTOMAG-70K), as previously described62 ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was extracted from the nodule powder following the protocol provided by the Spectrum™ Plant Total RNA-Kit (Sigma, https://www.sigmaaldrich.com). The total RNA was then converted to cDNA by reverse transcription with the SensiFAST™ cDNA Synthesis Kit (Bioline ...
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bioRxiv - Bioengineering 2024Quote: ... a smear from each strain was made on a glass microscope slide and stained with malachite green and safranin (Schaeffer-Fulton spore stain kit, Sigma-Aldrich, MO, USA) to visualize endospores ...
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bioRxiv - Cancer Biology 2024Quote: ... All cells were grown at 37°C in a humidified atmosphere containing 5% carbon dioxide (CO2) and were tested for mycoplasma contamination using the LookOut Mycoplasma PCR detection kit (Sigma; cat no. MP0035). The MIA PaCa-2 cell line was authenticated using short tandem repeat STR DNA profiling by a commercial testing facility (Genetica ...
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bioRxiv - Developmental Biology 2024Quote: ... Fixed cells were washed in distilled water and stained for alkaline phosphatase (AP) expression using a leukocyte alkaline phosphatase kit (Sigma, cat. 86R-1KT). Colonies were counted and categorised according to their morphology and alkaline phosphatase staining.
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bioRxiv - Plant Biology 2020Quote: ... total RNA was isolated from the base of spruce hypocotyls (5 mm long from 36 to 38 hypocotyl cuttings) using a Spectrum™ Plant Total RNA Kit (Sigma-Aldrich; https://www.sigmaaldrich.com/). cDNA was synthesized using an iScript™ cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... An equal concentration of cDNA from the samples comparing was used for qRT-PCR reaction with KAPA SYBR Fast qPCR Kit (Sigma-Aldrich, St. Louis, USA). For expression analysis of the genes responsive to nutrient starvation ...
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bioRxiv - Neuroscience 2020Quote: ... The RNA isolation of the retina was carried out according to the manufacturer’s introduction using the Gene Elute Mammalian Total RNA Miniprep Kit (Sigma-Aldrich, St. Louis, MO, USA). For total RNA isolation of optic nerve tissue ...
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bioRxiv - Cell Biology 2021Quote: ... the hiPSC-CMs were fixed and permeabilised with the FIX and PERM™ Cell Permeabilization kit and labelled with an antibody specific for α-actinin (1:250, Sigma-Aldrich #A7811), followed by an Alexa Fluor 350-conjugated secondary antibody (1:500 ...
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bioRxiv - Plant Biology 2020Quote: Total RNA was extracted from callus fragments grown on selective medium for eight weeks using a commercial kit (Sigma, Spectrum™ Plant Total RNA). First strand cDNA was synthesised using polyA primers and RevertAid First Strand cDNA synthesis kit (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: Nitrite concentration in the lung homogenate was measured (n=3 per group per time point) using Griess Reagent kit following manufacturer instructions (Sigma, St. Louis, MO, USA). Absorbance was recorded using a GloMax plate reader (Promega Corporation ...
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bioRxiv - Plant Biology 2021Quote: ... and ground to a fine powder (100-200 mg) prior to RNA isolation using the Spectrum™ Total Plant RNA Kit with DNase I digestion (Sigma, St. Louis, MO). The only modification to manufacturer’s ‘Protocol B’ was that prior to the tissue lysis step ...
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bioRxiv - Biochemistry 2022Quote: ... we first extracted the genomic DNA (gDNA) from each cellular fraction using the GenElute Mammalian Genomic DNA Miniprep kit (Sigma-Aldrich, St. Louis, MO). A previously described semi-nested polymerase chain reaction (PCR ...
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bioRxiv - Physiology 2022Quote: The mNGF level in tears upon a AAV2/9 CAG-mNGF injection in the LG was dosed using the mNGF ELISA kit (Sigma-Aldrich, reference RAB1119, France) following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: ... total cellular triglycerides were extracted from cells using the Folch extraction method and quantified by using a Serum Triglyceride Determination Kit (Sigma, St. Louis, MO, USA). The absorbance was read at 540nm using an Eppendorf Biospectrometer and 2.5 mg/mL glycerol was used as the standard (Sigma) ...
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bioRxiv - Microbiology 2020Quote: Cytokines in BAL fluid samples were measured (pg/ml) in singlicate by Luminex using a Millipore human cytokine multiplex kits (EMD Millipore Corporation, Billerica, MA) according to manufactures instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Each cellular fraction was then expanded and harvested prior to the extraction of genomic DNA using the GenElute Mammalian Genomic DNA Miniprep kit (Sigma-Aldrich, St. Louis, MO). A semi-nested PCR approach was then used to selectively amplify the mutagenized region of the polyprotein reporter from the genomic integration site in a similar manner as was previously described (Matreyek et al. ...
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bioRxiv - Microbiology 2021Quote: ... Then, compressed air (DAN oxygen kit, Divers Alert Network, USA) and 25 µM of sodium sulfide (Na2S*9H2O; Sigma-Aldrich, St. Louis, MS, USA) were injected into the oxic and anoxic incubations ...
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bioRxiv - Immunology 2020Quote: ADCP assays were performed using ExpiCHO-S target cells transiently transfected with SARS-CoV-2 S and fluorescently labeled with PKH67 Fluorescent Cell Linker Kits (Sigma Aldrich, Cat. Nr.: MINI67) as targets ...
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bioRxiv - Plant Biology 2020Quote: ... and DNA was extracted at the field using quick Extract-N-Amp™ Tissue PCR Kit (Sigma-Aldrich Co. LLC, MO, Louis. St; USA) with some modifications ...
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bioRxiv - Genomics 2021Quote: ... genotyping for the Ile148Met variant associated with hepatic steatosis (rs738409) was performed on genomic DNA extracted from the blood buffy coat using the GenElute Blood Genomic DNA kit (Sigma, St. Louis, MO, USA). rs738409 was genotyped using validated primers and TaqMan probes (Applied Biosystems ...
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bioRxiv - Immunology 2021Quote: ADCP was performed using CHO cells stably expressing full-length wild-type SARS-CoV-2 S glycoprotein (target cells) fluorescently labelled with PKH67 Fluorescent Cell Linker Kits (Sigma Aldrich; Cat. Nr.: MINI67). Target cells were incubated with titrated concentrations of mAbs for 10 minutes ...
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bioRxiv - Immunology 2021Quote: ... Ten μl were removed from each tube to measure C8 or longer free fatty acids (i.e., palmitic acid released from PGPC or POVPC) (Sigma free fatty acid kit MAK044) and the remaining samples were stored at -80℃ prior to use or analysis.
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bioRxiv - Evolutionary Biology 2022Quote: ... 20 females from each biological replicate were flash frozen in liquid nitrogen and total RNA was isolated using the GenElute Mammalian Genomic RNA miniprep kit (Sigma Aldrich, CAT# RTN350-1KT), following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... the nuclear fraction was prepared from whole plants using the ‘Semi-pure Preparation of Nuclei Procedures’ protocol of the CelLytic PN Isolation/Extraction Kit (Sigma-Aldrich, St. Louis, MO). Genomic DNA was isolated from the nucleus or whole plant using a DNeasy Plant mini kit (Qiagen ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... determined as the amount of 5-bromo-2′-deoxy-uridine (BrdU) incorporation (Roche BrDU Labeling and Detection Kit II, Sigma-Aldrich, St. Louis, MO). Briefly ...
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bioRxiv - Developmental Biology 2022Quote: ... offspring were screened for Akt1 or Foxo4 mutations from genomic DNA from tail-tip biopsies using the REDExtract-N-Amp™ Tissue PCR kit (XNAT, Millipore Sigma, Burlington, MA). PCR was performed on the purified DNA samples using primers flanking the guide RNA sites ...
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bioRxiv - Plant Biology 2022Quote: ... Full-length FIN4 mRNA in PCRII-TOPO vector was used to synthesize sense and anti-sense probes using a DIG-labeled probe synthesizing kit (Millipore-Sigma, catalog number 11175025910) with T7 RNA polymerase for sense probe and SP6 RNA polymerase for antisense probe synthesis with 1μg plasmid as template ...
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bioRxiv - Microbiology 2024Quote: PCR products obtained from the first round of amplification (COMPETE, BLOCK, CONTROL, see section 2.2) were purified using the GenElute™ PCR Clean-Up Kit (Sigma Aldrich, St. Louis, MO). The purified DNA was diluted 10-fold with DNA free sterile water and used as a DNA template for a nested PCR to attach Illumina adaptors for Illumina sequencing ...
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bioRxiv - Physiology 2023Quote: 25-30 mg of frozen powdered liver tissue was used to extract liver lipids as described previously 34 and reconstituted in a tert-butanol-Triton X solution (3:2) and liver triglycerides and cholesterol levels were measured via a commercially available kit (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Immunology 2024Quote: Total RNA was extracted from endothelial cells with the TRIzol reagent and Poly(A)+ RNA was purified using the GenEl-ute mRNA Miniprep Kit (Sigma, Louis, MO, USA; MRN10). The m6A concentration was assessed using the m6A RNA Methylation Kit (Abcam ...
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bioRxiv - Neuroscience 2023Quote: The TUNEL assay was performed on paraffin-embedded sections and fifty-micron-thick free-floating frozen sections according to the manufacturer’s instructions but with the time of some steps lengthened to ensure good penetration of the reagents into the tissue (ApopTag® Fluorescein In Situ Apoptosis Detection Kit, Millipore-Sigma, Cat # S7110). Sections were post-fixed for ten minutes at room temperature in 2:1 ethanol:acetic acid ...
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bioRxiv - Immunology 2023Quote: Cryopreserved blood plasma was thawed at room temperature and used for IL17a detection by a commercial mouse IL-17a ELISA Kit (Sigma Aldrich catalog #RAB0263-1KT). The test was performed according to manufacturer guidelines ...
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bioRxiv - Biophysics 2023Quote: ... were grown in ATCC’s vascular cell basal medium supplemented with microvascular endothelial cell growth kit-VEGF and 12.5 μg/mL blasticidine (Sigma-Aldrich, St. Louis, MO, 203351) for 48 h at 37°C + 5% CO2 until reaching 80-90% confluency ...
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bioRxiv - Plant Biology 2023Quote: RNA was extracted from leaf samples (three biological replicates for each genotype) and using Spectrum Plant Total RNA Kit (Sigma Aldrich, Saint Luis, USA) following instruction ...
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bioRxiv - Microbiology 2023Quote: ... bile salts were supplied from bovine bile and measured using a commercial kit according to the supplier’s protocol (Sigma-Aldrich, cat. No. MAK 309). Finally ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue lysis was performed in Roche cOmplete Lysis-M reagents (lysis buffer and protease inhibitor cocktail contained in the kit, Millipore Sigma cat. Nr. 4719956001). Hippocampi were placed in protein low-binding tubes mounted onto a Qiagen TissueLyser II (Qiagen cat ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein solution was dialyzed overnight at 4°C in tubes of the Pur-A-LyzerTM Maxi 6000 Dialysis Kit (Sigma, cat. No. PURX60100-1KT) against dialysis buffer (20 mM Tris ...
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bioRxiv - Cell Biology 2024Quote: ... 16 were subjected to in-situ Terminal deoxynucleotidyl Transferase dUTP Nick End Labeling (TUNEL) using the ApopTag Plus Fluorescein Kit (EMD Millipore, Burlington, MA, USA), following previously established protocols (Maddala et al. ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from 1.5-week-old Arabidopsis seedlings pooling 3 seedlings/extraction for each transgenic lines and using Spectrum™ Plant Total RNA Kit (Sigma-Al-drich, STRN250), RNAs were on-column treated with the DNase (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... Cytokines from the mouse brain homogenates prepared in Section 4.5 were measured using Millipore mouse cytokine multiplex kits (EMD Millipore Corporation, Billerica, MA, USA). Calibration curves from the recombinant cytokine standards were prepared by following threefold dilution steps in the same matrix as the samples ...
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bioRxiv - Immunology 2021Quote: ... At 0 min and 1 h the sample was analyzed for concentrations of inorganic monophosphates with the ATPase/GTPase Activity Kit (Sigma-Aldrich, Cat. no: MAK113-1KT) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... The PCR amplification for each gene sequence was performed with 50 ng of genomic DNA in a 20 µL reaction using a KAPA Taq PCR Kit (Sigma-Aldrich, St Louis, MO, USA) and the following cycle conditions ...
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bioRxiv - Plant Biology 2021Quote: ... 5’ RACE PCR was performed with 2 µl of 1:5 diluted cDNA in a 20 µl reaction with the KAPA2G Robust PCR Kit (KK5501, Sigma-Aldrich, St. Louis, Missouri, USA) buffer B ...
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bioRxiv - Plant Biology 2020Quote: ... they were dehydrated in an ethanol series (25, 50, 70, 100, and 100 %) and embedded in Epoxy resin using Epoxy medium kit (Sigma-Aldrich, St-Louis, MO, USA). Then ...
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bioRxiv - Genetics 2022Quote: ... Genomic DNA was extracted from pooled seedlings at the EBI laboratories using the GenElute Plant Genomic DNA Miniprep Kit (Sigma-Aldrich, St. Louis, MO, USA) following the manufacturer’s instructions ...