Labshake search
Citations for Millipore Sigma :
5551 - 5600 of 5739 citations for Heme Assay Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... using either a control normal rabbit IgG or rabbit monoclonal anti-MSI2 antibody and the Magna RIP RNA-binding Protein Immunoprecipitation kit (cat#17-700, Millipore, Burlington, MA). Manufacturer’s instructions were followed with the exception that RNeasy MinElute Cleanup kit (cat#74202 ...
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bioRxiv - Pathology 2023Quote: The SA-βgal activity was assessed in RPE cells at the end of the experiment using the Senescence Detection Kit (Sigma-Aldrich, #CS0030) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... ProteoExtract® native membrane protein extraction kit and 10 kDa Amicon® Ultra-15 centrifugal filters were purchased from Millipore Sigma (USA). Assays for measuring cell toxicity were purchased from Promega (Madison ...
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bioRxiv - Microbiology 2023Quote: ... All cell lines were tested every two weeks for mycoplasma contamination either by in-house DAPI staining or LookOut® Mycoplasma PCR Detection Kit (Sigma-Aldrich).
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bioRxiv - Genomics 2023Quote: ... Filtered nuclei suspension was pelleted as instructed and then resuspended in 50 ul Storage Buffer from the EZ nuclei kit (Sigma Aldrich, NUC101). For downstream processing ...
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bioRxiv - Cell Biology 2023Quote: ... and Vap33 and Git in Drosophila larval tissues were detected in situ using the Duolink® In Situ Red Starter Kit Mouse/Rabbit (Sigma, DUO92101) according to the instructions of the manufacturer ...
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bioRxiv - Biochemistry 2022Quote: ... and hearts), mouse organs (livers, brains, and hearts) and human hearts and brains was performed with a commercial kit (Sigma-Aldrich, 11814770001) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was isolated from overnight cultures of the wild type and mutant strains using GenElute Bacterial Genomic DNA Kits (Sigma-Aldrich, USA). Library preparation and whole genome sequencing were performed by Glasgow Polyomics ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were homogenized and then fractionated to obtain plasma membrane and cytosolic fractions using the ProteoExtract Subcellular Proteome Extraction Kit (Millipore, Billerica, MA). Antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... using either a control normal rabbit IgG or rabbit monoclonal anti-MSI2 antibody and the Magna RIP RNA-binding Protein Immunoprecipitation kit (cat#17-700, Millipore, Burlington, MA). Manufacturer’s instructions were followed with the exception that RNeasy MinElute Cleanup kit (cat#74202 ...
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bioRxiv - Genetics 2023Quote: ... blots were hybridized with Digoxigenin (DIG)-labeled DNA probes (Supplementary Table 2) amplified by the PCR DIG Probe Synthesis Kit (Sigma-Aldrich; 11636090910). The hybridized probe was immunodetected with anti-digoxigenin Fab fragments conjugated to alkaline phosphatase (Sigma-Aldrich ...
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bioRxiv - Genomics 2023Quote: ... The initial DNA amplification of the collected chromosomes was performed using a GenomePlex Single Cell Whole Genome Amplification Kit (WGA4) (Sigma-Aldrich, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were then dehydrated in a graded acetone series (30-100%) followed by resin embedding using an epoxy embedding kit (Fluka Analytical, Sigma Aldrich, Germany). Ultra-thin sections were obtained with a Leica EM UC6 (Leica Co ...
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bioRxiv - Molecular Biology 2023Quote: ... −5 °C for 4 minutes using the ZentriMix 380R (Hettich) and RNA was purified using the GeneElute™ Mammalian Total RNA Midiprep Kit (Sigma Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... were processed to isolate RNA starting from at least 50 mg of lyophilized tissue using the Spectrum™ Plant Total RNA Kit (Sigma-Aldrich) following manufacturer’s instructions (Nerva et al. ...
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bioRxiv - Plant Biology 2023Quote: ... were grown to late-log phase in photoautotrophic standard conditions for total RNA extractions using the Spectrum Plant Total RNA Kit (Sigma-Aldrich, STRN50) by following manufacturer instructions with the following modifications ...
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bioRxiv - Plant Biology 2023Quote: Genomic DNA of founders and RILs was extracted bulking young leaves of three seedling per genotype with the GenElute Plant Genomic DNA Miniprep Kit (Sigma Aldrich, Germany) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Live-cell AIS staining was performed using a mouse anti-neurofascin antibody targeting an extracellular epitope (NeuroMab clone A12/18, #75-172) conjugated with CF647 (Mix-N-Stain CF647 antibody labeling kit, Sigma-Aldrich #MX647S50) following the manufacturer protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were blocked in 1% BSA (in PBS) for 1 hour at room temperature and processed for PLA using the DuoLink in situ Red Starter Kit (Millipore Sigma #DUO92101) per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: We used T7 cysteine constrained heptapeptide (CX7C) phage libraries previously made in our lab(16, 18) using T7Select415-1 cloning kit (Novagen, catalog #70015) to select against CF pHBECs to discover mucus and cell-penetrating peptides ...
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ADEVO: Proof-of-concept of Adenovirus Directed EVOlution by random peptide display on the fiber knobbioRxiv - Bioengineering 2023Quote: ... viability measurements were performed for the 1 vpc and 3 vpc conditions of all vectors and two wells of uninfected cells with the Cell Counting Kit 8 (CCK8, Sigma-Aldrich #96992). Subsequently ...
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bioRxiv - Molecular Biology 2023Quote: ... The aortas at the aortic valve level were stained using an Elastica van Gieson staining kit (Sigma-Aldrich, Inc., St. Louis, MO) and Picrosirius red staining kit (Polysciences ...
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bioRxiv - Neuroscience 2024Quote: All cell cultures were tested for mycoplasma contamination once per month using LookOut® Mycoplasma PCR Detection Kit (Sigma, cat.no. MP0035-1KT).
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bioRxiv - Biochemistry 2024Quote: ... the supernatant was transferred to a fresh tube and total RNA was prepared using a GenElute total RNA purification Maxi kit (Sigma-Aldrich; RNB200) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... 0.4 µl 10 µM reverse tailed specific primer and 12.72 µl PCR grade water (contained in the Kap2G Robust PCR kit Sigma Catalogue No. KK5005) per sample and 18 µl added to each well to be used in a 96-well plate followed by 2 µl of DNA and mixed ...
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bioRxiv - Microbiology 2024Quote: ... All cell cultures and chlamydial stocks were routinely tested for Mycoplasma contamination using the Mycoplasma PCR detection kit (Sigma, St. Louis, MO). For E ...
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bioRxiv - Biochemistry 2024Quote: Total DNA was isolated form near-confluent fibroblasts or 18 DIV transduced neuronal cultures using a GeneElute Mammalian Genomic DNA Miniprep kit (Sigma, G1N350-1KT). Quantitative real-time PCR (StepOnePlus Real-Time PCR System ...
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bioRxiv - Pathology 2024Quote: Total RNA was extracted using the Spectrum™ Plant Total RNA Kit with the associated On-Column DNase I Digest Set (Sigma-Aldrich) to remove trace DNA ...
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bioRxiv - Physiology 2024Quote: ... and non-esterified fatty acids were measured with a commercial colorimetric kit according to manufacturer’s instructions as follows: pyruvate (Sigma Aldrich, MAK071, 10μL), amino acids (Abcam ...
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bioRxiv - Plant Biology 2024Quote: ... Approximately 0.1g of grounded plant material was used for total RNA extraction using Protocol A (750 μL Binding Solution) of the SpectrumTM Plant Total RNA Kit (Sigma, STRN-250) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... supernatants from the apical (or basolateral, where noted) side of the transwells were assessed using the Luminex MILLIPLEX Human Metabolic Hormone kit (EMD Millipore, USA) or using a serotonin ELISA (SER39-K01 ...
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bioRxiv - Microbiology 2024Quote: ... surviving bacteria that had grown up where pooled and chromosomal DNA from the input and output pools were isolated using the GenElute™ Bacterial Genomic DNA Kit (Millipore-Sigma). Library preparation followed a published protocol (55) ...
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bioRxiv - Neuroscience 2024Quote: ... The nuclei were thawed on ice for 2 min and lysed with EZ Lysis Buffer (Nuclei EZ Prep nuclei isolation kit, Sigma, #NUC101-1KT). The lysed sample incubated on ice for 5 min ...
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bioRxiv - Neuroscience 2024Quote: ... ACTH was measured by sandwich immunoassay using the MilliPlex MAP Mouse Pituitary Magnetic Bead Panel Kit (Merck Millipore; product code MPTMAG-49K). Corticosterone was measured using the Corticosterone EIA Kit (ImmunoDiagnosticSystems ...
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bioRxiv - Immunology 2024Quote: ... The resultant LNP formulations were dialyzed against PBS overnight at 4 °C by Pur-A-Lyzer™ Maxi Dialysis Kit (Millipore Sigma). After dialysis ...
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bioRxiv - Neuroscience 2024Quote: The cytokine concentrations of organoid lysate samples were quantified on the Luminex FLEXMAP3D platform using the Milliplex human cytokine and chemokine magnetic bead panel kit (Millipore HCYTOMAG-60K), measuring a broad panel of immune signaling proteins that allow for an unbiased survey of immune cues activating a diverse set of downstream intracellular pathways ...
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bioRxiv - Developmental Biology 2024Quote: ... and sectioned on a microtome to obtain 5 µm sections which were then stained using a Masson Trichrome staining kit (Sigma Aldrich, HT15) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2024Quote: LH and FSH were assayed in 10 μl of serum using the Luminex technology with the rat pituitary magnetic bead panel Milliplex Map kit (Merck-Millipore, Nottingham, UK) in accordance with the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Active GLP-1 release in STC-1 cell supernatant was detected with a GLP-1 (Active) ELISA kit (Millipore, Cat#: EGLP-35K). Samples were diluted 10-fold into kit-provided Assay Buffer prior to adding 200 μl sample/well ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was extracted according to the protocol of the SpectrumTM Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, United States). To remove any traces of DNA ...
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bioRxiv - Genomics 2024Quote: ... cells from 1 ml of culture were pelleted by centrifugation at 16000 x g for 2 minutes and DNA extracted using GenElute™ Bacterial Genomic DNA Kit (cat# NA2110, Sigma-Aldrich).
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bioRxiv - Cell Biology 2024Quote: ... Sections were stained for SA-β-gal activity using the CS0030 histochemical staining kit and counterstained using nuclear fast red (both Sigma-Aldrich) with or without anti-vimentin staining (1:200 ...
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bioRxiv - Cell Biology 2020Quote: ... PLA probes were applied in 1:10 dilution using the diluent buffer provided in the Duolink In Situ Red kit (Sigma Aldrich, cat# DUO92101). Incubations and subsequent ligation and amplification steps were performed according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The genotyping of the DNA extracted from ears of transgenic mice by Extract-N-Amp™ Tissue PCR Kit (Sigma St. Louis, MO) was performed following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... in the case of young oothecae (from NFE to 4-day-old) and GenElute Mammalian Total RNA Miniprep kit (Sigma-Aldrich, Madrid, Spain) in the case of older oothecae (from 6- to 16-day-old) ...
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bioRxiv - Molecular Biology 2022Quote: ... and 15 mg of the tissue from each mouse liver was used for genomic DNA extraction using GenElute Mammalian Genomic DNA Miniprep Kit (Millipore Sigma Cat. #: G1N350).
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bioRxiv - Cell Biology 2022Quote: ... were grown in ATCC’s vascular cell basal medium supplemented with microvascular endothelial cell growth kit-VEGF and 12.5 μg/mL blasticidine (Sigma-Aldrich, St Louis, MO) for 48 h at 37°C + 5% CO2 until reaching 80-90% confluence ...
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bioRxiv - Genomics 2020Quote: ... The lysates were then probed for phosphorylated STATs using the Milliplex map STAT cell signaling magnetic bead 5-Plex kit (Millipore Sigma, 48-610MAG) according to manufacturer’s protocol.
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bioRxiv - Neuroscience 2021Quote: Quantification of IL-6 in media was assessed via Human IL-6 ELISA kit according to manufacturer instructions (Sigma Aldrich; St. Louis, MO).
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bioRxiv - Plant Biology 2021Quote: ... Total RNA from ~100 mg of powdered tissue that was kept at −80 °C was extracted using Spectrum™ Plant Total RNA Kit (Sigma-Aldrich, USA) and treated with DNAse I (ThermoFisher Scientific ...