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Citations for Millipore Sigma :
5401 - 5450 of 10000+ citations for 6 PHENYL 1 3 DIHYDRO INDOL 2 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... The spheroplasts were then washed 3 times in SPM buffer permeabilized by incubation in 1 mL of 0.1% triton X-100 (Sigma) in PBS for 2 minutes on ice and washed twice in PBS ...
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bioRxiv - Plant Biology 2019Quote: ... 150 mM NaCl and 10 mM HEPES pH 7.4 and eluted with 100 μg ml−1 3×FLAG peptide (Sigma).
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bioRxiv - Molecular Biology 2019Quote: ... Bound proteins were cleaved off the column with thrombin diluted 1:250 in thrombin cleavage buffer (Novagen #69671-3). The thrombin solution was loaded manually into the column with a syringe ...
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bioRxiv - Immunology 2020Quote: ... After incubation cells were washed 3 times with PBS containing 0.5% BSA and subsequently fixed 1% (w/v) paraformaldehyde (PFA, Merck Millipore) in PBS ...
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bioRxiv - Plant Biology 2020Quote: ... epitopes were blocked with 0.1 M PBS / 1% Cold Water Fish Skin Gelatin / 0.2% BSA with 3% sucrose (all chemicals from Sigma Aldrich), incubated with the primary antibody anti-GFP (1:250 ...
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bioRxiv - Biochemistry 2020Quote: ... 50 mM sodium phosphate pH 8.0, 3% glycerol, 1% Triton X-100, 15 mM imidazole, and 1x protease inhibitor [Roche, Sigma]) and sonicated ...
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bioRxiv - Cell Biology 2021Quote: ... cells were trypsinized than fixed with PBS/ formalin 3% then incubated with PBS/ Triton 0.1%/ TRITC-phalloïdin 1 µg/mL (Sigma Aldrich) during 5 min ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were then washed twice with PBS prior to 20 min permeabilization with 0.5% Triton X-100 and 1 h blocking with 3% BSA (Sigma) at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... before 3 washes with 1 mL PBS and saturation with a 0.5% BSA solution in PBS 1X (Sigma A7888) for 15 min at RT ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was then incubated in blocking buffer for 1 hour at 25°C (TBST with 3% BSA; Sigma) before being probed for LapA or MapA containing an HA tag with an anti-HA antibody (Biolegend ...
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bioRxiv - Neuroscience 2020Quote: Sox14GFP/+ mouse brain samples (n=3) were embedded in a 4.5% oxidised-agarose solution containing agarose (type 1; Sigma), 10 mM NaIO4 (Sigma ...
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bioRxiv - Biophysics 2020Quote: ... The cultures were then blocked for 1 hour at room temperature with 3% (w/v) BSA in PBS (Sigma), followed by incubation with anti-Gfap (1:100 ...
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bioRxiv - Cell Biology 2021Quote: Chromatin was isolated from supernatant by adding 3 volumes of TE with 1% SDS and RNAse A (Sigma R6513), and vortexing to mix ...
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bioRxiv - Cell Biology 2021Quote: ... and HEK 293T cells were transfected with a 1:3 ratio of DNA:polyethylenimine (PEI, 25 kDa linear, Sigma-Aldrich). Transfection of siRNA was performed using X-tremeGENE siRNA Transfection Reagent (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transduced with sgRNA lentiviruses at an MOI of 3 with 8 µg ml−1 Polybrene (Merck-Millipore) for 4 hours and then reprogramming was initiated by addition of reprogramming medium ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated at RT for 1 hour in a blocking solution containing 3% Donkey Serum (Biowest) and 0.3% Triton X-100 (Sigma) in PBS 0.01M ...
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bioRxiv - Cancer Biology 2022Quote: Human melanoma cells were seeded in 6-well plates with 0.1-1 mL lentivirus in a final volume of 3 mL with 4 µg/mL polybrene (Millipore). Cells were spun at 1,000 x g for 2 h at 30 ºC ...
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bioRxiv - Immunology 2021Quote: ... at room temperature for 3 hours and followed by streptavidin-HRP (dilution 1:1000, Sigma-Aldrich, Cat# 18-152) for 45 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were blocked with 3% w/v BSA and incubated with 1:16,000 v/v rabbit anti-protein A antibody (SIGMA) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Band intensity was measured as the sum optical density by using ImageJ software (version1.4; NIH) and normalized to control labelling of glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:5000, Millipore).
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bioRxiv - Molecular Biology 2022Quote: ... 1-3 million cells were treated for 24 hours with 1.2 uM aphidicolin in PBS (Millipore Sigma cat#: A0781). Cells were attached to Concanavalin A coated slides for one hour at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... The primary antibody (1:100 Anti-Tau [3-repeat isoform RD3] Antibody, clone 8E6/C11, 05-803, Sigma-Aldrich and 1:100 Anti-Tau [4-repeat isoform RD4] Antibody ...
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bioRxiv - Microbiology 2022Quote: ... A total of 500 μL of normalized culture was mixed with 100 μL of 1% Zwittergent 3-14 (Sigma) in triplicate in 100 mM citric acid and incubated at 50 °C for 20 min ...
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bioRxiv - Cell Biology 2019Quote: ... 3% goat serum) and then incubated with mouse anti-tropomyosin-5 antibody (1:200; clone 2G10.2, Millipore, Burlington, MA) which recognizes Tpm3.1 and Tpm3.2 ...
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bioRxiv - Microbiology 2019Quote: ... 3 wells of parasites and 1 well of the RBC control were treated with 700nM dihydroartemisinin (DHA) (Sigma-Aldrich); an additional 3 wells of parasites and 1 well of RBC control was treated with an equivalent amount of dimethyl sulfoxide (DMSO ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Plasma and liver samples were prepared as previously described (18) with 3:1 ice-cold acetonitrile and 2.5µM chlorpropamide internal standard (Sigma Aldrich) then run on both the Waters ACQUITY UPLC HSS T3 (1.8µm particle size ...
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bioRxiv - Bioengineering 2019Quote: ... Samples were then washed 3 times with TBS containing 1% BSA and 0.1% w/v sodium azide (Sigma Aldrich). After staining ...
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bioRxiv - Neuroscience 2019Quote: ... aggregates were cultured in 3:1 medium supplemented with an additional 10% heat-inactivated FBS (Millipore, #es‒009‒b) and 100 µM taurine (Sigma ...
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bioRxiv - Developmental Biology 2021Quote: ... EpiSCs were passaged 1:4–1:10 every 3 days by triturating the colonies into small clumps using 0.5 mg ml–1 collagenase IV (Sigma). MEK/WNT-inhibited RSCs were cultivated with 1,000 U ml–1 LIF ...
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bioRxiv - Microbiology 2020Quote: ... 1 mM DTT) and treated with 3×45 s bead beating with glass beads (particle size≤106 μm Sigma) at maximum power and cooling on ice between the cycles (MP Biomedicals™ FastPrep-24™ ...
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bioRxiv - Microbiology 2021Quote: ... The reaction was initiated at the spectrophotometer by adding anoxic xanthine oxidase (microbial; 1-3 µg/mL; Sigma-Aldrich) via syringe and needle through the septum lid ...
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bioRxiv - Developmental Biology 2020Quote: Cryosections (30-40 μm) were blocked with PBS/1-3% normal goat or donkey serum/0.3% Triton X-100 (Sigma) for up to 1 hour at room temperature (RT ...
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bioRxiv - Genetics 2020Quote: Total RNA from 7 DAP spikes was extracted using TRI Reagent and 1-bromo-3-chloropropane (BCP) (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... Cell pellets were lysed in Qiagen “Phosphoprotein lysis buffer” containing 0.25% (v/v) CHAPS with 1% (v/v) phosphatase inhibitor cocktail 3 (Sigma), protease inhibitor tablet (Qiagen ...
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bioRxiv - Developmental Biology 2019Quote: ... embryos in the chorion were embedded into 1% low-melting agarose with optional 0.016% Ethyl 3-aminobenzoate methanesulfonate salt (Tricaine, Cat#A5040; Sigma) in E3 embryo medium ...
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bioRxiv - Genetics 2021Quote: ... a mouse anti-glyceraldehyde-3-phosphate dehydrogenase monoclonal antibody (#MAB374, used at 1:10,000 for the Western blot analyses, Merck Millipore); a rabbit anti-LC3B polyclonal antibody (#2775 ...
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bioRxiv - Cell Biology 2020Quote: ... permeabilized with 0.1% Triton X-100 in PBS for 3 min and blocked in PBG (PBS, 0.045% cold fish gelatin (Sigma), and 0.5% BSA) ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were positioned on gelatin-coated slides to allow further development with 3:1 ammonia solution (1054261000, Milipore Sigma) for 8 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... 293T or AL1 cells were incubated for 3 hours with anti-Myc mouse monoclonal antibody (Sigma-Aldrich; 1:500), and secondary staining was performed using anti-mouse AlexaFluor568 antibodies (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... cells were fixed in 1% PFA (10 min, 37°C) and permeabilized in permeabilization buffer (PBS 3% BSA, 0.1% saponin, Sigma) for 5 min ...
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bioRxiv - Genetics 2020Quote: ... incubated for 3 hours at 25 °C with the secondary antibody (anti-rabbit IgG peroxidase 1:10000, Sigma-Aldrich; anti-mouse IgG peroxidase 1:10000 ...
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bioRxiv - Immunology 2022Quote: ... and blocked for 1 hour at room temperature with 100 μL of 3% globulin free BSA (Sigma-Aldrich, A7030). After blocking ...
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bioRxiv - Developmental Biology 2022Quote: ... fused gastruloids were induced by administration of 3 μM CHIR and if indicated with doxycycline (1 μg/μl, Sigma) for 24 h ...
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bioRxiv - Biochemistry 2022Quote: ... The incubated beads were washed 3 times with HAC buffer followed by eluted in 1 M imidazole (Sigma, #IX0005) in HAC buffer.
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bioRxiv - Cell Biology 2022Quote: ... The beads were washed with IP lysis buffer and incubated with 5 mg ml−1 3× FLAG peptide (Sigma–Aldrich ...
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bioRxiv - Plant Biology 2022Quote: ... The transformants were then spotted on SD (−Trp, −Leu, −His) selection media containing 1 mM 3-amino-1,2,4-triazole (Sigma, A8056). The positive interactors were then scored based on the stringency of the selection.
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bioRxiv - Systems Biology 2023Quote: Buffy Coat was diluted with PBS at a 1:3 dilution and underlayed with Histopaque®-1077 (Sigma-Aldrich), centrifuged at 350g for 30 minutes at 24°C with no brake ...
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bioRxiv - Neuroscience 2023Quote: ... 100 μg/mL streptomycin and 1 mM N6,2′-O-Dibutyryladenosine 3′,5′-cyclic sodium monophosphate salt (dbcAMP) (Sigma®). Cells were maintained with the differentiation medium at 37 °C in 5% CO2 for 3 days.
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bioRxiv - Physiology 2022Quote: ... cells were treated with the medium supplemented with 0.5 mM 3’-Isobutyl-1-methylxanthine (IBMX) (Sigma, Cat. I7018-1G), 1 µM dexamethasone (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed 3 times with DPBS for 5 min and blocked in 1% BSA (Sigma-Aldrich, cat# A4503) in PBS-T (DPBS with 0.05% Tween 20 ...