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Citations for Millipore Sigma :
5151 - 5200 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... adherent human embryonic kidney (HEK) 293T cells were transfected with the expression plasmids with branched polyehtyleneimine (PEI, Sigma-Aldrich). In detail ...
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bioRxiv - Cell Biology 2021Quote: ... Human cells were trypsinized and incubated in cold PBS supplemented with 5% fetal calf serum (Sigma-Aldrich; PBS-FACS). DNA was stained either by propidium iodide (PI) ...
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bioRxiv - Cancer Biology 2021Quote: ... Chip channels were then coated with a mixture of ECM in Dulbecco’s phosphate-buffered saline (DPBS): 200μg/mL human placenta collagen type IV (Sigma-Aldrich) and 30 μg/mL fibronectin (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... and the cleavage fragments of C3b were detected by immunoblotting with a polyclonal anti-human C3b antibody (HPA020432; Sigma) (1:1000) ...
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bioRxiv - Cancer Biology 2020Quote: ... The human glioma initiating cells (GS7-11, GS8-11, GSC280) were maintained in serum-free DMEM/F12 medium (Sigma), containing B27 (Invitrogen) ...
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bioRxiv - Cancer Biology 2020Quote: ... Black Falcon clear flat bottom 96-well plates were coated with 5 μg/mL human collagen I (Sigma-Aldrich) in 2 mM acetic acid (Merck) ...
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bioRxiv - Cancer Biology 2020Quote: Glioma cells were grown as attached on cell-treated vessels coated with 10ug/mL human plasma fibronectin (Millipore, FC010) and transfected with X2 (Mirus Bio ...
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bioRxiv - Cell Biology 2021Quote: ... 6μl of 10μM siRNAs targeting human KLC1 or KLC2 were mixed with 12μl HiPerFect in high-glucose DMEM medium (Sigma-Aldrich) and combined with 1×105 cells per well of a 6-well plate ...
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bioRxiv - Cell Biology 2021Quote: ... and MDA-MB-231 (triple-negative human breast adenocarcinoma) cells were grown in DMEM (Dulbecco’s Modified Eagle’s Medium with HEPES modification; Sigma, D1152) supplemented with 10 % fetal bovine serum (FCS ...
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bioRxiv - Molecular Biology 2022Quote: Human embryonic kidney epithelium 293T (HEK293T) cells and Hela cells were cultured in DMEM-high glucose (Sigma-Aldrich, USA) supplemented with 10% fetal bovine serum (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... hiPSCs were plated at a density of 4×104 to 5×104 cells/cm2 onto a human fibronectin (Millipore)-coated 12-well plate in GK15 medium (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... PBS was replaced with a solution of TCNB buffer (50mM Tris, pH7.5, with 100mM NaCl, 10mM CaCl2) containing 89.5 nM human MMP9 (Sigma SAE0078) and incubated at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... THP-1 (ATCC® TIB-202™) human monocyte cell line was cultured in RPMI-1640 medium (Sigma, R8758) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2021Quote: Human adenocarcinoma alveolar basal epithelial cells (A549, ATCC) were maintained in high-glucose Dulbecco’s Modified Eagle Medium (DMEM, Sigma) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2019Quote: Human colorectal adenocarcinoma cell lines HT29 and HCT116 (both European Collection of Cell Cultures through Sigma-Aldrich, Dorset, UK) were grown in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Biochemistry 2021Quote: The hnRNP L_RRM2 fragment (residues189-286) was amplified from the human brain cDNA library and cloned into a modified pET28a (Novagen) vector without a thrombin protease cleavage site (termed p28a) ...
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bioRxiv - Biophysics 2021Quote: The human esaHis-tagged PTPN11 (residues 1-528) cDNA was cloned in a pET-26b vector (Novagen, MA, USA). Nucleotide substitutions associated with NS or leukemia were introduced by site-directed mutagenesis (QuikChange site-directed mutagenesis kit ...
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bioRxiv - Neuroscience 2020Quote: ... Plates were washed with PBST and incubated with a goat anti-human Fc−peroxidase antibody diluted 1:5,000 (Sigma) in blocking buffer for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... The plates were then washed and incubated with a 1:5,000 dilution of peroxidase-conjugated anti-human Fc antibody (Sigma) for 1 hr ...
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bioRxiv - Cancer Biology 2021Quote: ... digested splenic cells were then cultured in MEM-α with 5% human platelet lysates (PLTMax; Cat# SCM142, Millipore Sigma) at a density of 2-5 × 104 cells/mL in culture flasks for 24 hours ...
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bioRxiv - Neuroscience 2022Quote: ... 50% Neurobasal Medium, 0.5% N2 supplement, 1% B27 without vitamin A [Life Technologies, 12587-010], 0.025% [v/v] human insulin [Sigma, I9278] ...
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bioRxiv - Cancer Biology 2022Quote: ... human tumor slides were stained by immunohistochemistry with an antibody against Tenascin-C (1:300, EMD Millipore, Cat. ab19011), a strain-sensitive ECM protein known to be present in LUAD [9 ...
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bioRxiv - Cancer Biology 2022Quote: ... HEK293 (human embryonic kidney, ATCC) and U2OS (osteosarcoma, ATCC) cells were grown in DMEM (Dulbecco’s modified Eagle’s medium, Sigma-Aldrich) supplemented with 10% FBS and 2 mM L-glutamine.
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bioRxiv - Bioengineering 2019Quote: ... The antibodies used were mouse anti-human HSA (1:500; clone HSA-11, A6684; Sigma Aldrich; St. Louis, MO) and 10 nm colloidal gold-conjugated goat anti-mouse IgG (1:40 ...
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bioRxiv - Pathology 2020Quote: ... the suspension adjusted to 1 × 106 parasites/mL in PBS and was incubated with 20% human complement (Sigma Aldrich). After 30 min ...
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bioRxiv - Systems Biology 2020Quote: ... Metabolic hormones were measured using MILLIPLEX MAP Human Metabolic Hormone Magnetic Bead Panel - Metabolism Multiplex Assay (HMHEMAG-34K, Millipore). Cardiovascular risk markers in plasma were measured using MILLIPLEX MAP Human Cardiovascular Disease (CVD ...
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bioRxiv - Biochemistry 2019Quote: ... ti-sodium citrate and sodium hydroxide were purchased from Merck Company and Human serum albumin (96%) and ANS (1-anilino-8-naphthalene sulfonic acid) were purchased from Sigma-Aldrich.
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bioRxiv - Cell Biology 2020Quote: Human 293T and mouse P19 cells were cultured in Dulbecco’s modified Eagle’s medium (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... The cGAS knockdown for RNA interference was achieved using Mission shRNA-encoding lentivirus directed to human cGAS mRNA (Sigma; GenBank/EMBL/DDBJ accession no ...
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bioRxiv - Molecular Biology 2020Quote: Human FOP fibroblasts (Coriell Institute, NJ) were grown to confluence in DMEM supplemented with 10% FBS (Sigma-Aldrich, MO) and then seeded into 96-well plates (Costar® 3610 ...
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bioRxiv - Molecular Biology 2021Quote: Human genomic DNA was isolated from HeLa cells using a GenElute™ Mammalian Genomic DNA Miniprep Kit (Sigma-Aldrich). Target genes were PCR amplified from genomic DNA using Phusion® High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... at 14:30 and 48 hours later with 5 IU of human chorionic gonadotropin (5 IU, hCG; EMD Millipore). At the time of hCG injection ...
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bioRxiv - Genetics 2020Quote: ... The cauda epididymis was nicked three times to allow sperm to swim out and placed in a 2ml round bottom Eppendorf tube filled with 1.1ml of 37°C Human Tubal Fluid media (Millipore). Sperm were placed in a 37°C incubation chamber and allowed to swim out for 10 minutes before the cauda epididymis was removed ...
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bioRxiv - Immunology 2019Quote: ... Cells were blocked overnight at 4°C with 150µl blocking solution per well (5% (v/v) human serum (Sigma), 5% (v/v ...
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bioRxiv - Neuroscience 2019Quote: ... pH = 7.6) containing 0.01% Triton X-100 (TBS-T) and then blocked with 2% human serum albumin (HSA; Sigma-Aldrich) and 10% NGS in TBS-T for 30 min ...
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bioRxiv - Bioengineering 2019Quote: ... or 48-well plates (with or without 7×7mm glass coverslips) pretreated with 50 µg/mL human fibronectin (Millipore).
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bioRxiv - Neuroscience 2019Quote: After extensive washes in PB and 0.05 M Tris-buffered saline (pH 7.4; TBS) sections were blocked in 1 % human serum albumin (HSA; Sigma-Aldrich) in TBS ...
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bioRxiv - Immunology 2020Quote: Human monocyte-derived macrophages (hMDMs) were differentiated from buffy cones from healthy donors using gradient separation (Histopaque 1077, Sigma). Following Histopaque separation ...
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bioRxiv - Microbiology 2020Quote: ... Non-adherent cells were removed and the remaining monocytes were maintained in DMEM with 5% human serum (HS; Millipore) and penicillin/streptomycin ...
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bioRxiv - Cancer Biology 2021Quote: ... or a non-targeting control shRNA (TRCN0000231489) with no targets in the human genome were obtained from Sigma-Aldrich. The SLFN11 expression vector was obtained from VectorBuilder along with an empty vector control with the same lentiviral backbone ...
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bioRxiv - Biochemistry 2021Quote: ... Primary rabbit polyclonal anti-(human Nav1.5), (residues: 1978-2016) (#ASC-013, Alomone Labs) and rabbit polyclonal anti-(actin) (A2066, Sigma), both used at a concentration of 1:200 were followed by a one-hour incubation with secondary Goat anti-(Rabbit IgG-HRP conjugate ...
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bioRxiv - Immunology 2021Quote: ... The albumin analyte was selected and measured using MILLIPLEX MAP Human Kidney Injury Magnetic Bead Panel 2 (Millipore Sigma) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Female frogs (4-15 years old) were injected subcutaneously with 300-700 units of human chorionic gonadotropin (hCG; Sigma), depending on weight and age ...
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bioRxiv - Developmental Biology 2020Quote: ... sperm were collected from the vasa deferentia and then capacitated for 1.5 hours in human tubal fluid (HTF) medium (Millipore). Sperm cells were then incubated with intact cumulus-oocyte complexes or zona-free oocytes for 5 hours ...
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bioRxiv - Microbiology 2020Quote: HEK293 cells (a human embryonic kidney cell line; ATCC CRL-1573) were maintained in Dulbecco’s modified Eagle’s medium (Sigma-Aldrich) containing fetal calf serum and antibiotics.
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bioRxiv - Microbiology 2020Quote: ... Non-adherent cells were removed and the remaining monocytes were maintained in DMEM with 5% human serum (HS; Millipore) and penicillin/streptomycin ...
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bioRxiv - Immunology 2021Quote: ... The bound antibodies were revealed using a HRP-conjugated goat antibody to mouse Ig absorbed with human Ig (Sigma) (1:4,000 dilution) ...
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bioRxiv - Immunology 2021Quote: ... in conjunction with pLKO.1 lentiviral vector targeting GFP or human c-Rel at a ratio of (1.5:3:4) using X-tremeGENE HP DNA transfection reagent (Sigma). 48h and 72h later viral particle containing media was harvested ...
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bioRxiv - Immunology 2021Quote: ... 0.3 x 106 primary human keratinocytes were seeded directly after transfection in 500 µl keratinocyte medium supplemented with 1.5 mM CaCl2 (SIGMA) into the insert ...
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bioRxiv - Immunology 2020Quote: ... were incubated overnight at 4°C with 50 μL of a solution of in 75 mM sodium carbonate (pH 9.6) containing either: 2.5 μg/ml aggregated human IgG (Sigma-Aldrich) for CP ...