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Citations for Millipore Sigma :
5251 - 5300 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Human plasma like media (HPLM) was generated according to the published formulation59 with addition of 5% dialyzed FBS (Sigma) and Pen/Strep (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: Lentiviral vectors containing validated shRNA sequences targeting expression of human PRKCD (accession NM_006254) or a non-target control were obtained from Sigma (catalog numbers TRCN0000010193 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell were then fixed as previously described and all three batches were permeabilised with Triton X-100 0.5% in PBS and a Cy5-conjugated anti-human (AC112S, Sigma) was used for the detection of T-DM1 and T-DXd ...
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bioRxiv - Cell Biology 2023Quote: ... Cell pellets were seeded onto plasticware coated with a solution of 0.1 % gelatin containing 10 µg/ml human plasma fibronectin (FN) (Millipore). and collagen type 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Normal human peripheral blood mononuclear cells (PBMCs) (FUJIFILM Wako Pure Chemical, Osaka, Japan) were cultured in RPMI1640 (Sigma-Aldrich) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2023Quote: Human mini-colons cultured in biological triplicates were extracted from the PDMS chips and digested with collagenase I (Sigma) at 100 U/ml concentration in Advanced DMEM/F12 with penicillin/streptomycin ...
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bioRxiv - Microbiology 2024Quote: ... with 25 mM HEPES and (iii) RPMI with 25 mM HEPES and supplemented with 10% human serum (Sigma-H4522). Then ...
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bioRxiv - Cell Biology 2024Quote: ... The small interfering siRNA (siRNA) oligonucleotide targeting human FCHSD2 (5’-GCAUACUCCUGAGACCUCA[dT][dT]-3’) was obtained from Sigma Aldrich. FCHSD2 siRNA knockdown in all HeLa cell lines was performed for 48 h using the DharmaFECT transfection reagent (Dharmacon ...
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bioRxiv - Cell Biology 2024Quote: ... 3,3’ s-dihexyloxacarbocyanine iodine (DiOC6) and fibrinogen from human plasma were obtained from Sigma Aldrich (St. Louis, Missouri, USA). ML161 (PAR1 inhibitor ...
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bioRxiv - Genetics 2024Quote: ... cells were transferred into SFEM II supplemented with 3 U/mL hEPO (SCT) and 3% AB human serum (Sigma) for an additional 5 days of culture ...
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bioRxiv - Microbiology 2024Quote: Human neutrophils from the peripheral blood of healthy blood donors were isolated by density gradient centrifugation (Histopaque; Sigma–Aldrich) followed by hypotonic lysis of erythrocytes ...
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bioRxiv - Cell Biology 2024Quote: ... and human breast cancer cells (4175-LuT; Hoshino et al., 2015) were cultured in Dulbecco’s modified Eagle’s medium (Sigma‒ Aldrich) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Human GBM commercial cell lines were maintained routinely in Dulbecco’s Modified Eagle Medium (DMEM) (Sigma, New York, NY, USA), supplemented with 5% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... The 3dGRO™ human ISO72 colorectal cancer tumoroid line was purchased from Millipore Sigma (Burlington, MA, USA, cat# SCC507) and cultured in vendor-specified homebrew media ...
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bioRxiv - Neuroscience 2024Quote: ... Then sections were washed in PB and 0.05 M Tris-buffered saline (pH 7.4; TBS) and blocked in 1% human serum albumin (Sigma-Aldrich) in TBS ...
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bioRxiv - Bioengineering 2024Quote: ... and glucagon were measured using a customized MILLIPLEX human metabolic Hormone Panel V3 (Millipore Sigma, cat. no. HMH3-34K). CCL2 ...
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bioRxiv - Cell Biology 2024Quote: Madin-Darby canine kidney cells with stable expression of human α-2,6-sialyltransferase (MDCK-SIAT1) (Sigma, catalog #: 05071502-1VL), human lung adenocarcinoma epithelial A549-Dual cells (InvivoGen ...
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bioRxiv - Cell Biology 2024Quote: ... human embryonic kidney HEK-293 T cells and human dermal fibroblasts (HDFs) were cultured in Dulbecco’s Modified Eagle’s medium (DMEM, Sigma-Aldrich), supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2024Quote: ... The proximity ligation reaction and visualization of signal was performed according to the manufacturer’s protocol using the Duolink Detection Kit with PLA PLUS and MINUS probes for rabbit and human antibodies (Sigma). DAPI stain was used to detect cell nuclei ...
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bioRxiv - Biochemistry 2024Quote: His6-tagged CCT domains (mouse SPAK 459-556 and human OSR1 433-527) and mouse SPAK 62-556 T243E were expressed in Rosetta (DE3) E.coli (Novagen). Cells were grown at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... T2 cells were incubated with a titration of each tested peptide and 100 ng/mL human β2-microglobulin (Sigma) in serum-free medium (TexMacs ...
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bioRxiv - Microbiology 2024Quote: ... Chips were coated overnight at 4°C with ECM composed of 200 ug/mL of human Collagen IV (Sigma) + 100 ug/mL of Matrigel (Corning) ...
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bioRxiv - Biophysics 2021Quote: ... The labeled protein concentration was measured using the Direct Detect spectrometer (Merck Millipore). The degree of labeling was calculated as the ratio of the dye concentration and the protein concentration ...
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bioRxiv - Biophysics 2021Quote: ... Proteins were transferred onto an Immobilon FL PVDF membrane (Merck Millipore, Darmstadt, Germany), probed with antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... Supernatants containing chromatin fragments were pre-cleared with protein A agarose beads (Millipore) for 20 min and incubated with a specific antibody overnight at 4 °C on a nutator (antibody information were listed in Supplementary Table 1).then added 50 ul of protein A agarose beads and incubated for 2 h ...
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bioRxiv - Cancer Biology 2021Quote: ... Completeness of protein transfer was determined by Ponceau S (Sigma-Aldrich, cat# P7170) staining for 5-6 min followed by frequent washing with H20 ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were immunoprecipitated by incubating cell extracts overnight with either FLAG M2 (Sigma) or GFP (Clonetech ...
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bioRxiv - Cell Biology 2019Quote: ... Total extracted proteins (20 μg) were derivatized according to the manufacturer’s instructions (Millipore). 2-mercaptoethanol was used to reduce samples ...
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bioRxiv - Cell Biology 2019Quote: ... S-protein-agarose beads and S-tag antibody were purchased from EMD Millipore. Endo H ...
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bioRxiv - Cell Biology 2020Quote: Protein loading quantity was controlled using a monoclonal anti-α-tubulin antibody (Sigma). Secondary antibodies were from Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were then transferred to Immobilon-P transfer membranes (PVDFs) (IPVH00010; Millipore). The membranes were blocked with 5% non-fat milk in Tris-buffered saline containing 0.1% Tween-20 (TBST ...
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bioRxiv - Cell Biology 2020Quote: Primary antibodies against the following proteins were used: actin (rabbit, Sigma-Aldrich, #A2066), cMyc (mouse ...
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bioRxiv - Cell Biology 2019Quote: ... 2 μl lambda protein phosphatase (800 U) and buffer components as instructed (Sigma) and the reaction was terminated after 30 min at 30 °C ...
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bioRxiv - Cell Biology 2020Quote: ... 300µg of proteins were incubated with 30µl of Streptavidin-Agarose beads (Sigma, CS1638) on a rotating wheel at 4°C for 3hr ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins samples were resolved by SDS-PAGE and transferred to PVDF membranes (Millipore) for Western blot analysis.
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bioRxiv - Immunology 2021Quote: ... The deglycosylated protein was desalted and concentrated using Amicon filter (Merck Millipore, USA). The native protein and enzyme treated proteins were separated through SDS-PAGE and the proteins were transferred to the Nitrocellulose membrane using Trans-Blot Turbo Transfer System (Bio Rad) ...
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bioRxiv - Genetics 2021Quote: ... 1 mM phenylmethylsulfonyl (PMSF) and 1% cocktail protein protease and phosphatase inhibitors (Sigma), pH 8.0) ...
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bioRxiv - Cell Biology 2020Quote: ... the supernatant was mixed with 30 μL of protein A Sepharose (Sigma P3391) and rotated for 2 h at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Flag-tagged proteins were purified by immunoprecipitation with anti-Flag M2 antibody (Sigma) and Protein G Mag Sepharose (GE healthcare) ...
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bioRxiv - Microbiology 2019Quote: ... inner-membrane proteins were solubilized using 0.5% (w/v) N-lauryl sarcosine (Sigma) in a buffer containing 20 mM Tris-HCl [pH 7.5] and 150 Mm NaCl for 1.5 h at 4 °C on a roller ...
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bioRxiv - Immunology 2021Quote: ... and filtering through 0.65 µm low protein binding filter plates (Millipore, Burlington, USA).
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bioRxiv - Microbiology 2021Quote: ... Purified protein was further concentrated using Amicon Ultra Centrifugal Filter Units (Merck Millipore).
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bioRxiv - Developmental Biology 2021Quote: Proteins were transferred to 0.45 μm Immobilon-P PVDF membrane (Millipore, Burlington, MA) in transfer buffer (25 mM Tris ...
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bioRxiv - Immunology 2021Quote: ... Priming was done with 50μg of protein in complete Freund’s adjuvant (Sigma, India) per mouse ...
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bioRxiv - Developmental Biology 2020Quote: ... Proteins associated with 3xFLAG-tagged MBD3 were purified using anti-FLAG sepharose (Sigma) and processed for mass spectrometry as described (Smits et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... 50μL of pre washed Magna ChIP protein A+G magnetic beads (Millipore Sigma) were then added to the samples and rotated at 4°C for 2 hours to elute the chromatin:antibody complexes ...
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bioRxiv - Immunology 2022Quote: ... were coated with 20μg/ml OVA protein (Sigma Aldrich, St Louis, MO, USA) or 4μg/ml CII (Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... The protein was eluted with 100 μg/ml of 3×FLAG peptide (Sigma). The eluted protein was adjusted to desired concentration via Vivaspin (Sartorius ...
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bioRxiv - Biophysics 2022Quote: ... gels were stained for 1 h using ReadyBlueTM Protein Gel Stain (Sigma-Aldrich) and destained with ddH2O overnight ...
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bioRxiv - Molecular Biology 2019Quote: ... and proteins were eluted by boiling with Sample Buffer Laemli 2X (Sigma-Aldrich) for 5 min at 95°C ...