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Citations for Millipore Sigma :
5301 - 5350 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The captured DNA-RNA hybrids were enriched by protein A-agarose beads (Millipore). The purified DNA was used to construct a library with the NEXTflex Rapid DNA-seq Kit (PerkinElmer).
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bioRxiv - Molecular Biology 2020Quote: ... the protein is transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore Corp., MA, USA) at 20 V ...
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bioRxiv - Molecular Biology 2020Quote: ... The eluates were incubated with 40 μl of S-protein-agarose beads (Millipore) for 2 hr at 4 °C and then washed three times with NETN buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were separated on 6% polyacrylamide gel and transferred on PVDF membrane (Millipore) using semi-dry blotting ...
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bioRxiv - Cancer Biology 2020Quote: ... FLAG-tagged fusion proteins were immunoprecipitated using Anti-FLAG M2 magnetic beads (Sigma) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Fractions containing protein were concentrated in 50-kDa-cutoff Amicon centrifugal filters (Millipore). Aliquots were frozen in dry ice and stored at -80 °C ...
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bioRxiv - Bioengineering 2022Quote: ... Eluted proteins were buffer exchanged and concentrated with PBS using Amicon tubes (Millipore). The proteins were further purified by size exclusion chromatography (SEC ...
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bioRxiv - Cell Biology 2022Quote: ... Bound proteins were eluted with 125 ng/μL 3xFLAG peptide (Sigma-Aldrich, F4799) in TBS ...
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bioRxiv - Developmental Biology 2022Quote: ... Supernatants were precleared with 20 μL of protein A/G magnetic beads (Millipore) at 4°C for 1 hour ...
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bioRxiv - Microbiology 2022Quote: ... the protein content and purity were evaluated with SDS-PAGE and InstantBlue (Sigma) gel staining (Extended Data Fig ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were then visualized with ProteoSilver Plus Silver Stain Kit (Sigma Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... proteins were concentrated on a 0.5 mL 10 kDa filter (UFC501024, Merck-Millipore) by ultracentrifugation and purity was controlled by SDS-PAGE.
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bioRxiv - Cancer Biology 2019Quote: ... The respective protein bands were visualized using the immunobilion chemiluminescence system (Millipore, MA). The densitometry analysis of immunoblotting images was performed using Quantity One Software (Bio-rad) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Protein levels were normalized to β-actin (1:5000 dilution, A5060, Sigma Aldrich). Membranes were washed and incubated with the appropriate horseradish peroxidase-conjugated secondary antibody for 1 h at room temperature and detected by chemiluminescence system (GE Healthcare Europe GmbH ...
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bioRxiv - Cell Biology 2019Quote: ... Samples were precleared by a 30 minute-incubation with Protein G agarose (Sigma), and then incubated for 2 hours at 4°C with Protein G agarose and 5 µg of indicated antibodies ...
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bioRxiv - Biochemistry 2019Quote: ... and the protein concentration was assessed using a BCA kit (Sigma-Aldrich, USA). Samples were diluted to equal concentrations and separated using the Novex Native PAGE Bis–Tris Gel system (4–16% ...
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bioRxiv - Immunology 2019Quote: ... Proteins were detected using Luminata™ Forte Western HRP Substrate (Millipore, Watford, UK) visualized on Hyperfilm™ (GE Healthcare ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated in the blocking buffer (5% non-fat milk ...
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bioRxiv - Immunology 2019Quote: ... SDS-PAGE separated proteins were transferred onto Immobilon-FL PVDF membranes (EMD Millipore) at 100V for 1h at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoreactive proteins were visualized by Immobilion western chemiluminescence substrate (Merck-Millipore cat#WBKLS0500) [7].
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bioRxiv - Biophysics 2020Quote: ... and 10 μL of 5 mg∙mL−1 of protein A (Sigma-Aldrich), were added to 1 mL of 10 mg∙mL−1 of N-(3-dimethylaminopropyl)-N’-ethylcabodiimidie hydrochloride (EDC ...
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bioRxiv - Immunology 2020Quote: Protein lysates were subjected to SDS-PAGE and transferred to PVDF membranes (Millipore). Membranes were blocked and probed with one of the following antibodies ...
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bioRxiv - Bioengineering 2019Quote: ... Antibodies were purified from medium using protein G gravitrap (Sigma, 28-9852-55) according to manufacturer protocol ...
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bioRxiv - Microbiology 2019Quote: ... proteins precipitated on the beads were eluted using Laemmli’s buffer containing dithiothreitol (Sigma) boiled 5 min at 95 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... protein synthesis was inhibited by treating cells with 350 µM cycloheximide (Sigma Aldrich).
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bioRxiv - Cell Biology 2019Quote: ... and DNA loaded proteins were released by incubation with S7 nuclease (Sigma Aldrich) in complete CSK at room temperature (RT ...
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bioRxiv - Immunology 2019Quote: ... Streptomyces hyaluronlyticus hyaluronidase (HA’se) and biotinylated HA-binding protein (HABP) were from Millipore, and streptavidin was from Thermo Fisher Scientific or eBioscience ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were detected by western blot using anti-FLAG (1:1,000 dilution; Sigma), anti-hemagglutinin (HA ...
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bioRxiv - Molecular Biology 2019Quote: ... FLAG-CTCF-6xHis protein was induced in Rosseta-Gami B-cells (EMD Millipore) with 0.2 M of IPTG at room temperature and was purified with Ni-NTA resin (Qiagen ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was then concentrated with a 10K Amicon Ultra-15 concentrator (Millipore) and diluted into Storage Buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2020Quote: CD200 and 41BB FAST proteins were digested overnight with TEV protease (Sigma # T4455) at 4 °C in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... anti-microtubule associated protein 2 (MAP2, 1:500, mouse monoclonal, Sigma, St.Louis, MO), GluN2B (1:1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein fractions were isolated and concentrated using 3kDa centrifugal filters (Merck Millipore). LC MS/MS was performed on the concentrated culture CMed and spectral analysis was performed ...
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bioRxiv - Biochemistry 2019Quote: ... and protein concentration was measured using a Bradford assay (Sigma-Aldrich, Taufkirchen, Germany). Proteins were separated with a 10% SDS-PAGE ...
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bioRxiv - Plant Biology 2020Quote: ... cells were harvested and lysed using BugBuster Protein Extraction Reagent (Novagen, Nottingham, UK), soluble fractions were used over gravity flow Ni2+-NTA sepharose columns (1 ml ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Total proteins were spotted (10 μg) onto nitrocellulose membranes (Merck Millipore, Darmstadt, Germany) and left to dry ...
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bioRxiv - Genomics 2019Quote: ... and then protein bands were detected using Horseradish peroxidase-conjugated secondary antibodies (Sigma) and Clarity Western ECL Substrate according to manufacturer’s instructions (BioRad) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Total proteins were spotted (10 μg) onto nitrocellulose membranes (Merck Millipore, Darmstadt, Germany) and left to dry ...
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bioRxiv - Neuroscience 2019Quote: ... and the proteins were transferred to a pre-activated PVDF membrane (Millipore, IPFL00005) at −70 mV for 3 to 4 hours ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were transferred to a polyvinylidene difluoride (PVDF) membrane (EMD Millipore, Toronto, ON), and blocked in 5% skim milk or BSA ...
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bioRxiv - Cell Biology 2019Quote: ... Total protein bands were reversibly stained with Fast Green FCF (Sigma-Aldrich, F7252) and visualized on ImageQuant LAS 4000 (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was eluted using purification buffer supplemented with 5 mM D-desthiobiotin (Sigma). Following affinity purification ...
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bioRxiv - Developmental Biology 2021Quote: ... The proteins were transferred to a polyvinylidene fluoride (PVDF) membrane (Sigma-Aldrich, ISEQ00010) and blocked in 5% fat-free milk for one hour at room temperature ...
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bioRxiv - Plant Biology 2020Quote: ... proteins were eluted with 150 μg.ml-1 3× FLAG peptide (Sigma-Aldrich, F4799) in extraction buffer ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... FLAG and Myc tagged-proteins were detected with mAb-FLAG M2 (Sigma-Aldrich) and mAb-Myc 1-9E10.2 (ATCC) ...
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bioRxiv - Biochemistry 2021Quote: ... bound protein was eluted with binding buffer containing 2.5 mM d-Desthiobiotin (Millipore) and dialyzed into dialysis buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... The Gag-His6 protein was loaded onto a HIS-select affinity column (Sigma), washed (20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was treated with > 10-fold excess of TCEP in DMSO (Sigma) and incubated for an hour in the anaerobic chamber at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... The concentrated protein samples were filtrated through Millex 0.22-μm syringe filters (Millipore) and loaded onto a Superdex 200 HR 10/30 gel-filtration column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was concentrated with a 100 MWCO Amicon centrifugal filter unit (Millipore) and purified by size exclusion chromatography using a Superose 6 Increase 10/300 GL column (GE Healthcare ...