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Citations for Millipore Sigma :
5151 - 5200 of 10000+ citations for C C Motif Chemokine Ligand 23 CCL23 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... 800 µL of culture was layered over 700 µL cold silicone oil (9:1 AR20/Sigma High Temperature, cooled to -78°C) and cells pelleted at 13,000 r.c.f ...
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bioRxiv - Immunology 2021Quote: ... Concentrations were determined in duplicate by fluorescent-coded magnetic beads (MagPlex-C; MILLIPLEX. MAP Kit, Canine Cytokine Magnetic Bead Panel, 96-Well Plate Assay, CCYTO-90K, Millipore, Billerica, MA), based on the Luminex xMAP technology (Luminex 200 ...
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bioRxiv - Biophysics 2020Quote: FAK activity of MEFs (Extended Data Fig. 10b, c) was inhibited by incubating the cells with 1 μM PF573228 (Sigma-Aldrich, PZ0117) for 1 hours ...
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bioRxiv - Genetics 2022Quote: ... cells were grown at 30°C in SC complete medium until mid-log phase (0.6 OD600) and incubated with nocodazole (Sigma-Aldrich; Catalogue # M1404) (15μg/ml ...
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bioRxiv - Cell Biology 2022Quote: ... cells were seeded in 6-well plates the previous day and treated either with 200ug/ml Cycloheximide (CHX) (Sigma-Aldrich # C-7698) in fresh growth media for 2 ...
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bioRxiv - Neuroscience 2022Quote: ... and the cochleae were fixed in 4% paraformaldehyde (PFA) overnight at 4 °C and then decalcified in 10% EDTA (#798681, Sigma, Darmstadt, Germany) in PBS for 2–3 days at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... cleared in propylene oxide and embedded in a mixture of Epon 812 and Durcupan (Sigma; polymerization for 3 days at 60°C). Firstly ...
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bioRxiv - Immunology 2022Quote: ... the retrovirus supernatant was concentrated by centrifugation at 5000g at 4 °C for 2 h with Amicon Ultra-15 Centrifugal Filter Units (Millipore, Billerica, MA). A human T cell line (Jurkat ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were next subjected to gelation by incubation for 1 hr at 37°C with monomer solution [19% Sodium Acrylate (Sigma Aldrich: 408220), 10% Acrylamide ...
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bioRxiv - Cell Biology 2022Quote: ... Centrifuge the cell lysate at 21,130g for 30 min at 4°C and carefully transfer the supernatant to a pre-build iodixanol step gradients (15%, 25%, 40% and 58%, Sigma-Aldrich, D1556)) for ultracentrifugation purification at 41,000 rpm for 4 hours ...
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bioRxiv - Systems Biology 2020Quote: ... Tissue was then roughly chopped into ~5 mm3 cubes and incubated at 37°C for 1 hour in 1 mg/ml of filter-sterilized collagenase type IA-S from Clostridium histolyticum (Sigma-Aldrich C5894) in DMEM ...
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bioRxiv - Molecular Biology 2019Quote: ... coli DH5α was used for all cloning experiments and was grown in LB medium (60) at 37°C with Ampicillin (Sigma Aldrich, USA) at 100 µg/ml.
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bioRxiv - Neuroscience 2019Quote: ... c-Fos counts were blindly performed immunohistochemically for staining of cocaine (10mg/kg; i.p.)-induced rabbit anti-c-Fos (1:10K, EMD Millipore, MA, USA).
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bioRxiv - Immunology 2019Quote: ... 24 and 48 hours and then fixed with 4°C methanol for 3 minutes and stained with safranin (Sigma-Aldrich, Gilligham, UK) for 10 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... The cytotrophoblasts between densities of 1.049 g/mL and 1.062 g/mL were collected and plated in fibronectin (10 μg/mL)-coated cover glass for culture at 37°C in 5% CO2-95% air in DMEM containing 10% fetal calf serum (Sigma, cat. F2442) and 1% antibiotics (Gibco ...
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bioRxiv - Genomics 2020Quote: ... and tissue was enzymatically digested in a solution (1X HBSS, 20mM HEPES, 30mM sodium bicarbonate, 1% BSA fraction V) containing collagenase type IV (200 U/mL; Sigma C-5138); hyaluronidase Type IS (1mg/mL ...
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bioRxiv - Neuroscience 2019Quote: ... cortical sections were incubated overnight at 4 °C with anti-glial fibrillary acidic protein (GFAP, 1:1000, chicken IgG, Merck Millipore, Burlington, EUA), anti-Iba1 (1:200 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... calcein (C, >95%), D-cycloserine, fluorescein (F, 95%), fluorescein-5-isothiocyanate (FITC, 90%) and TRITON® X-100 were purchased from Sigma-Aldrich. Ethanol (for UV spectroscopy ...
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DJ-1 (Park7) affects the gut microbiome, metabolites and development of Innate Lymphoid cells (ILCs)bioRxiv - Immunology 2019Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences; GAPDH: #5174, Cell Signaling and GFAP #MAB360, Merck Millipore, Germany). After incubation with the first antibody ...
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bioRxiv - Biochemistry 2020Quote: ... and the cells were grown overnight at 37 °C on LB agar plates containing 50 μg/ml of kanamycin (Sigma Aldrich, USA). A single colony was selected ...
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bioRxiv - Microbiology 2021Quote: ... The membranes were washed three times with PBS-T at 4°C and the specific proteins were visualized with a chemiluminescence HRP substrate system (Millipore, Massachusetts, USA) using LAS 4000 (Fujifilm Life Science ...
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bioRxiv - Microbiology 2020Quote: ... Samples were incubated with 2.9 mM dithiothreitol at 60°C for 30 minutes followed by addition of 7.9 mM iodoacetamide (Sigma-Aldrich, St. Louis, MO) at room temperature for 30 minutes ...
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bioRxiv - Microbiology 2021Quote: Huh7 and VeroE6 cells were grown at 37°C and 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) (Sigma-Aldrich, St. Louis, MO) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2019Quote: ... Specimens for histamine immunohistochemistry were prefixed overnight at 4°C in 4% N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDAC, Sigma–Aldrich E6383). Brains were washed six times for 15 minutes each in PBS and subsequently immersed in Poly-L-Lysin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... or with a solution of glycerol and DMSO in PB and were frozen with −60/-70°C isopentane (2-methyl butane, Sigma-Aldrich, Germany) (following the protocol of Rosene et al ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were grown in T75 flasks in a humidified atmosphere of 5% CO2 in air at 37°C and removed from the flask surface using incubation with trypsin (0.05% w/v) (Sigma Aldrich, Poole, UK) containing EDTA (0.03% w/v ...
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bioRxiv - Genomics 2021Quote: ... mESCs (J1) were initially cultured in presence of Mitomycin C inactivated mouse embryonic fibroblasts on 0.1% gelatin-coated (Millipore, ES-006-B) 6-well plates in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Evolutionary Biology 2020Quote: Whole animals (~100 hatchlings) were dissociated by incubating for 1h at 25°C in a collagenase solution (1mg/mL, Sigma-Aldrich C9722) with continuous agitation ...
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bioRxiv - Physiology 2019Quote: ... gels were permeabilised (0.2% Triton X-100) in 1 × TBS for 90 mins and incubated overnight (4°C) in 250 μl Phalloidin-FITC (Sigma Aldrich, P5282) at a concentration of 50 μg/ml ...
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bioRxiv - Developmental Biology 2019Quote: ... Fertilized embryos were injected before first cleavage on agarose pads in a solution of pasteurized (30 minutes 65°C) filtered seawater (PFSW) + 2% w/v Ficoll 400 (Sigma F-9378). Embryos were hand-sorted for fluorescence between second and sixth cleavage cycles ...
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bioRxiv - Neuroscience 2019Quote: ... At this point we modified the protocol to incubate the samples in tert-Butanol incubation for 12 hrs at 35 °C followed by immersion in dichloromethane (Sigma-Aldrich, 270997) for 12 hrs at room temperature and finally incubation with the refractive index matching solution BABB (benzyl alcohol + benzyl benzoate 1:2 ...
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bioRxiv - Synthetic Biology 2019Quote: ... were separated on an Ascentis Express Peptide ES-C18 column (2.7 µm particle size, 160 Å pore size, 50 mm length × 2.1 mm i.d., 60 °C; Sigma-Aldrich, St. Louis, MO) by using a chromatographic gradient (400 µL/min flow rate ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse embryonic fibroblast were obtained at P0 and cultivated in DMEM (High Glucose and Pyruvate) plus 10% serum until P4 then treated with 10 µg/mL mitomycin C (Sigma-Aldrich; M428).
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bioRxiv - Genomics 2021Quote: ... then the cells were lysed with Hi-C lysis buffer (10mM Tris-HCl pH8.0 10mM NaCl, 0.2% NP-40) with 1X of protease inhibitors (Sigma complete protease inhibitor cocktail) and the nuclei were recovered ...
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bioRxiv - Microbiology 2020Quote: ... was grown at 37°C in Luria-Bertani (LB) broth (Difco, Becton Dickinson, MD, USA) supplemented with 100 µg/ml ampicillin (Sigma-Aldrich, USA) or 50 µg/ml kanamycin to express 6xHIS-tagged Hsp18 or MUL_3720 recombinant protein ...
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bioRxiv - Cell Biology 2020Quote: ... sheets of 0.3–0.4 mm thickness were prepared with an electrodermatome and were treated overnight at 4°C with 0.2% (wt/vol) dispase II (Boehringer Mannheim, Mannheim, Germany) in PBS with 50 μg/ml gentamycin (Sigma, St. Louis, MO). Epidermis and dermis were separated by forceps ...
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bioRxiv - Microbiology 2020Quote: ... Cells were further cultured for 72 h at 37°C with 5% CO2 and positively transduced cells were selected using puromycin (5 µg/ml Sigma-Aldrich, Poland). Selected cells were plated on insert supports and further cultured in ALI in the presence of puromycin (1 µg/ml).
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bioRxiv - Microbiology 2020Quote: ... Cells were further cultured for 72 h at 37°C with 5% CO2 and positively transduced cells were selected using blasticidin (2 µg/ml Sigma-Aldrich, Poland). Primary human epithelial cells seeded on 10 cm2 dishes were cultured in BEGM medium and transduced with lentiviral particles harboring shRNA against KLK13 (a set of 3 ...
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bioRxiv - Physiology 2021Quote: ... midguts were incubated for 1h30 at room-temperature or overnight at 4°C in 10 μM Fluorescein Isothiocyanate (FITC) (Sigma-Aldrich #P5282) or Texas-Red labeled phalloidin (Invitrogen TM #T7471) ...
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bioRxiv - Physiology 2021Quote: For actin staining midguts were incubated for 1h30 at room-temperature or overnight at 4°C in 10 μM Fluorescein Isothiocyanate (FITC) (Sigma-Aldrich #P5282) or Texas-Red labeled phalloidin (Invitrogen TM #T7471) ...
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bioRxiv - Plant Biology 2020Quote: ... One-STrEP-tagged kinases purified from Arabidopsis epidermal peels or 0.2-1 μg of kinases overexpressed in bacteria was incubated (25 min at 30°C or as indicated) with 2.5 μg of MBP (Millipore, Temecula, CA, USA) or 0.5 μg of another substrate protein ...
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bioRxiv - Cell Biology 2020Quote: ... The cleared lysate was then incubated for 2h at 4 °C with 10 μl of anti-FLAG M2 Magnetic beads (Sigma-Aldrich, M8823) pre-washed with TBS ...
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bioRxiv - Biophysics 2020Quote: ... nuclei were incubated with blocking solution (10% goat serum in PBS) for 30 min at 37°C and stained with mouse-anti-γH2AX (1/500, Millipore 05-636) for 1 hr at 37°C followed by O/N incubation at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... HCECs were grown in a hypoxia chamber with 2% O2 and 5% CO2 at 37°C in 4 parts DMEM to 1 part medium 199 (Sigma-Aldrich #M4530) with 2% cosmic calf serum (GE Healthcare) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The second aliquote was frozen on dry ice and stored at −80 °C until usage in the RNA extraction by TRI Reagent® (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... (ECACC) were cultured at 37°C in 5% humidified CO2 in high glucose Dulbecco’s Modified Eagle Medium (DMEM, Sigma or GE Healthcare), supplemented with 4 mM L-glutamine ...
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bioRxiv - Genomics 2021Quote: ... cells were first washed once with phosphate-buffered saline (PBS) and supplemented with equilibrated (37 °C and 5% CO2) medium containing 500 μM indole-3-acetic acid (Sigma-Aldrich, I5148) freshly prepared before use ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNA fragment encoding mouse C/EBPβ residues 221–296 (bZIP domain) was cloned into pET-45b(+) vector (Novagen/Merck, Darmstadt, Germay) via ‘in vivo assembly’ (35 ...
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bioRxiv - Microbiology 2020Quote: ... and COS7 cells were maintained at 37°C and 5% CO2 in Dulbecco’s Modified Minimum Eagle Medium (DMEM) (Sigma-Aldrich, MO, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2019Quote: ... JW18 cell lines infected with the three strains of Wolbachia were cultured in a 75 cm2 flask in 12 ml Schneider’s medium supplemented with 10% FBS at 28 °C (Sigma Aldrich, NSW, Australia). The Haematobia embryonic cell line (HIE-18 ...