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Citations for Addgene :
251 - 300 of 472 citations for NGS ChIP Seq kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... plasmid only or additionally with 125 ng of Lyn11-FRB-mCherry plasmid (Addgene, #38004) using Lipofectamine 3000 (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2019Quote: ... the 70-80% confluent cells were transfected with 780 ng psPAX2 (Addgene plasmid #12260), 510 ng pMD2.G (Addgene plasmid #12259 ...
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bioRxiv - Neuroscience 2022Quote: ... cells were co-transfected with 750 ng of pCMV-PE2 Plasmid (Addgene Plasmid #132775) and 250 ng of assembled pU6-pegRNA-GG-acceptor using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... pDD162 (50 ng/μl, to express Cas9; a gift from Bob Goldstein, Addgene #47549) (Dickinson et al. ...
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bioRxiv - Immunology 2024Quote: ... grown in wells of 6-well plates with 1000 ng psPAX2 (Addgene plasmid #12260) packaging plasmid ...
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bioRxiv - Developmental Biology 2021Quote: ... and 2.5 ng/mL Pmyo-2::mCherry as a co-injection marker (pCFJ90, Addgene #19327). Microinjection of adult N2 hermaph-rodites was performed as described above ...
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bioRxiv - Neuroscience 2020Quote: ... All transfections contained mCherry-CD9-10 (250 ng, was a gift from Michael Davidson; Addgene plasmid #55013 ...
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bioRxiv - Molecular Biology 2020Quote: ... and pHsp70-Cas9 (52) (250 ng/μl) (Addgene plasmid # 60944; http://n2t.net/addgene:60944; RRID:Addgene_60944) into dechorionated fly embryos ...
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bioRxiv - Immunology 2021Quote: ... previously transfected with 500 ng of a human ACE2 expression plasmid (Addgene, Cambridge, MA, USA) were seeded at a density of 2 × 104 in 100 µL DMEM-10% in a white flat-bottomed 96-well plate one day prior to harvesting SARS-CoV-2 pps ...
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Therapeutic base and prime editing of COL7A1 mutations in recessive dystrophic epidermolysis bullosabioRxiv - Bioengineering 2021Quote: ... 150,000 patient-derived fibroblasts were transfected with 900 ng of PE2-encoding plasmid (Addgene #132775), 300 ng of pegRNA-encoding plasmid ...
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Therapeutic base and prime editing of COL7A1 mutations in recessive dystrophic epidermolysis bullosabioRxiv - Bioengineering 2021Quote: ... 150,000 patient-derived fibroblasts were transfected with 900 ng of ABEmax-encoding plasmid (Addgene, #112095). For prime editing ...
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bioRxiv - Immunology 2020Quote: ... previously transfected with 500 ng of a human ACE2 expression plasmid (Addgene, Cambridge, MA, USA) were seeded at a density of 2 × 104 in 100 µL DMEM-10% in a white flat-bottomed 96-well plate one day prior to harvesting of SARS-CoV-2 pps ...
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bioRxiv - Microbiology 2020Quote: ... HEK293T on 10-mm coverglasses were transfected with 200 ng of pcDNA3.1-hACE2 (Addgene, #145033) 24 hours later ...
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bioRxiv - Immunology 2020Quote: ... previously transfected with 500 ng of a human ACE2 expression plasmid (Addgene, Cambridge, MA, USA) were seeded at a density of 2 × 104 in 100 μL DMEM-10% in a white flat-bottomed 96-well plate one day prior to harvesting SARS-CoV-2 pps ...
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bioRxiv - Microbiology 2023Quote: ... cells were co-transfected with 900 ng of the I-SceI plasmid (pCBASceI, Addgene #26477) and 900 ng of pcDNA4/TO-HHV-6B IE1 (+I-SceI ...
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bioRxiv - Immunology 2023Quote: ... cells were transfected with 500 ng psPAX2 (a gift from Didier Trono, Addgene plasmid #12260), 250 ng VSV-G (a gift from Didier Trono ...
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bioRxiv - Immunology 2023Quote: Constructs encoding base editors NG-CBE3.9MAX (gift from John Doench and David Root, Addgene #179095) and NG-ABE8e (gift from David Liu ...
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bioRxiv - Cell Biology 2020Quote: ... NG-ABEmax-encoding plasmid wasconstructed in our lab based on the appropriate backbone plasmids (Addgene # 112095).
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bioRxiv - Cancer Biology 2019Quote: ... Then cells were transfected with 100 ng of pTsgRNA-DMP-Cas9-EGFP or pEGFP-C1 (Addgene) using Lipofectamine® 2000 Reagent (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2020Quote: U2OS cells were cultured and transfected with 100-200 ng of mEmerald-Tomm20 plasmid (Addgene, 54281) directly on the BIO-133 bottomed well plate ...
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bioRxiv - Bioengineering 2021Quote: ... and assembled in a Golden Gate assembly containing 6.25 ng pU6-atgRNA-GG-acceptor (Addgene #132777), purified PCR product (approximately 2-to-4-fold molar excess) ...
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bioRxiv - Cell Biology 2020Quote: ... by LR clonase reaction of pEF5/FRT/NG-DEST with pENTR-PRKAR1A (obtained from Addgene, #23741). IMCD3 cells stably expressing GPR161NG have previously been described (Ye et al. ...
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bioRxiv - Biophysics 2020Quote: ... HEK293T cells on the wells were co-transfected with 1250 ng of ACE2 (Addgene plasmid #141185) and 1250 ng of TMPRSS2 expression plasmids (Addgene plasmid #145843 ...
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bioRxiv - Immunology 2020Quote: ... 2 × 104 HEK293T target cells transfected with 500 ng of a human ACE2 expression plasmid (Addgene) were seeded in a white flat-bottom 96-well plate one day prior to the assays ...
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bioRxiv - Bioengineering 2023Quote: ... Wells were transfected with 45 ng PPRE luciferase (a gift from Bruce Spiegelman; Addgene plasmid #1015) and 5 ng renilla control reporter vector (Promega) ...
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bioRxiv - Neuroscience 2023Quote: ... and 0.5 ng/μl of the Olig2 CRISPR plasmid (pX458-Ef1a-Cas9-H2B-mCherry, Addgene #171101). The electroporation of embryos was achieved by securing each head between circular tweezer-electrodes (5 mm in diameter ...
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bioRxiv - Developmental Biology 2023Quote: ... the lentiviral packaging plasmids 800 ng pMD2.G (was a gift from Didier Trono, Addgene #12259) and 2400 ng psPAX2 (was a gift from Didier Trono ...
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bioRxiv - Neuroscience 2024Quote: ... Plasmid based prime editing: 500 ng pCMV-PE2-GFP (a gift from David Liu, Addgene#132776)115 ...
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bioRxiv - Synthetic Biology 2023Quote: ... The YTK kit (Addgene; Kit #1000000061) was used as a source for all relevant non-coding DNA sequences — including promoters and terminators ...
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bioRxiv - Genetics 2019Quote: ... HEK293T cells were transfected with a mixture of 100 ng of plasmid encoding sgRNA (pRG2; Addgene #104174) and 100 ng of plasmid encoding SpCas9 (pRGEN-Cas9-CMV/T7-Puro-RFP ...
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bioRxiv - Genetics 2020Quote: ... The yeast were then transformed with 100 ng of CRISPR-Swap plasmid (pFA0055-gCASS5a, Addgene plasmid # 131774) and 1 μg of DNA repair template amplified either from BY ...
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bioRxiv - Biochemistry 2021Quote: A 20 μL reaction containing 150 ng of a plasmid containing two loxP sites (Addgene Plasmid #26852) and 500 nM of Cre or Cre mutants in recombination buffer (50 mM Tris-Cl ...
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bioRxiv - Cell Biology 2020Quote: ... 400,000 U2OS cells were transiently co-transfected with 200 ng gRNA expression plasmid (cloned into Addgene 43860), 500 ng Cas9 expression plasmid (Addgene 43945) ...
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bioRxiv - Developmental Biology 2022Quote: ... was used for PCR reactions with 30-50 ng of pScarlessHD-DsRed plasmid as template (Addgene #64703) or 50 ng of gBlocks or genomic DNA from the same Drosophila strain (BDSC #55821) ...
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bioRxiv - Molecular Biology 2022Quote: ... and cells were transfected with 500 ng of CAG-Cas9-T2A-EGFP-ires-puro-plasmid (Addgene, #78311) and with 250 ng of gRNAs altogether using PEI transfection reagent with addition of 0.15 M NaCl ...
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bioRxiv - Developmental Biology 2023Quote: PCR amplification of the repair template was performed using 50 ng of plasmid containing mNeonGreen (Addgene 125134) as template in a touchdown PCR reaction (annealing temperature decreased 1°C from 65°C to 50°C for the first 15 cycles followed by 20 cycles with an annealing temperature of 50°C ...
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bioRxiv - Biophysics 2023Quote: ... Transfections contained 70 ng of SpCas9 nuclease plasmid (pCMV-T7-SpCas9-P2A-EGFP (RTW3027; Addgene ID 139987) and 15 ng each of two sgRNA expression plasmids mixed with 0.3 µL of TransIT-X2 (Mirus ...
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bioRxiv - Synthetic Biology 2022Quote: ... MoClo Plant Parts Kit (Addgene Kit #1000000047), GreenGate Cloning System (Addgene Kit #1000000036 ...
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bioRxiv - Developmental Biology 2021Quote: ... The injection mix was prepared in MilliQ H2O and contained 60 ng/mL Peft-3::Cas9 (Addgene #46168), 45 ng/mL each sgRNA plasmid ...
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bioRxiv - Neuroscience 2020Quote: ... All transfections contained mCherry-CD9-10 (250 ng, was a gift from Michael Davidson; Addgene plasmid #55013; http://n2t.net/addgene:55013; RRID:Addgene_55013) to visualize neurons and spiny protrusions (Figure 1) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cells within each well were transfected with 50 ng of the LLPS1-iLID::EGFP::FTH1 (Addgene plasmid #122149) construct containing the core FTH1 scaffolding domain attached to iLID for light-inducible interactions ...
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bioRxiv - Genomics 2022Quote: ... Per transfection reaction we used 7.5ul of 1 mg/mL polyethyleneimine (PEI) and 2.325 μg of total plasmid DNA (825 ng psPAX2: Addgene #12260 ...
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bioRxiv - Cell Biology 2019Quote: ... cells were transfected with 200 ng of a biosensor for Rac1 activation (pTriEx4-Rac1-2G; Addgene plasmid # 66110) (Fritz et al. ...
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bioRxiv - Genetics 2022Quote: ... and pBS-Hsp70-Cas9 (140 ng/µL, from Melissa Harrison & Kate O’Connor-Giles & Jill Wildonger, Addgene plasmid #45945) as the source of Cas9 for transformation ...
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bioRxiv - Developmental Biology 2021Quote: ... Injection mixes were prepared in MilliQ H2O and contained 50 ng/ml Peft-3::cas9 (Addgene ID #46168) (Friedland et al. ...
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bioRxiv - Genetics 2019Quote: ... and pBS-Hsp70-Cas9 (140 ng/µL, from Melissa Harrison & Kate O’Connor-Giles & Jill Wildonger, Addgene plasmid #45945) providing Cas9 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and pBS-Hsp70-Cas9 (~500 ng/μL, from Melissa Harrison & Kate O’Connor-Giles & Jill Wildonger, Addgene plasmid #45945) providing Cas9 ...
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bioRxiv - Biochemistry 2022Quote: ... and HTT 2088-3144 was mixed with 600 ng of the destination vector pDEST_gateway-2xFLAG (Addgene plasmid #118372), 1 μl of LR clonase ...
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bioRxiv - Cell Biology 2023Quote: ... 1 ng of pcDNA3.3-hCas9 plasmid (constructed by inserting the Cas9 fragment released from Addgene #41815 (ref. 119), into the pcDNA3.3 vector ...
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bioRxiv - Molecular Biology 2023Quote: ESCs were co-transfected with 1000 ng of pX458 SpCas9(BB)-2A-GFP vector (Addgene #48138, contains eGFP)80 and 500 ng of vector co-expressing four sgRNAs to the Peg13 DMR CTCF region (contains DsRed ...