Labshake search
Citations for Addgene :
101 - 150 of 472 citations for NGS ChIP Seq kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... and 100 ng pMD2.G (Addgene, #12259) with TransIT-Lenti (Mirus ...
-
bioRxiv - Genetics 2023Quote: ... and 150 ng of dnMLH1 (Addgene #174824) were used for transfection ...
-
bioRxiv - Genetics 2023Quote: ... and 1100 ng of PAX2 (Addgene #12260) plasmid using polyethylenimine (PEI ...
-
bioRxiv - Molecular Biology 2023Quote: ... 250 ng of actin-GAL4 (Addgene #24344) and 250 ng of UAS:dCas9-CtBP effectors were co-transfected in 6-well plates ...
-
bioRxiv - Biochemistry 2024Quote: ... Super8xTOPFLASH (Addgene plasmid #12456, 80 ng/well) and LacZ under a CMV promoter (20 ng/well ...
-
bioRxiv - Molecular Biology 2024Quote: ... 200 ng of hMbPylRS-4xU6M15 (Addgene, #105830) and 200 ng of the respective amber construct were premixed in 20 µL of DMEM ...
-
bioRxiv - Biochemistry 2023Quote: ... 1400 ng of psPAX2 (Addgene plasmid #12260), 140 ng of pMD2.G Addgene plasmid #12259) ...
-
bioRxiv - Genomics 2021Quote: ... DNA fragments were subsequently cloned into the STARR-seq screening vector (pSTARR-seq_human, Addgene plasmid #71509) using the In-Fusion® HD Cloning Kit (Takara/Clonetech) ...
-
bioRxiv - Molecular Biology 2023Quote: ... as well as overhangs for assembly into the STARR-seq vector (Addgene #99296; Supplemental Table 1) according to the manufacturer’s instruction (10 to 11 cycles of amplification) ...
-
bioRxiv - Genomics 2022Quote: ... Per transfection reaction we used 7.5ul of 1 mg/mL polyethyleneimine (PEI) and 2.325 μg of total plasmid DNA (825 ng psPAX2: Addgene #12260; 550 ng pMD2.G: Addgene #12259 ...
-
bioRxiv - Neuroscience 2019Quote: ... Site-directed integration into attP sites was achieved by co-injection of an attB-containing vector (400 ng µl−1) and either p3xP3-EGFP.vas-int.NLS (400 ng µl−1) (Addgene #60948)69 or pBS130 (encoding phiC31 integrase under control of a heat shock promoter ...
-
bioRxiv - Developmental Biology 2023Quote: ... animals were injected with a target transgene (50 ng μl-1) with plasmid pJL43.1 (50 ng μl-1) (Addgene) that expresses MosTase under a germline promoter and plasmid pMR910 (20 ng μl-1 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cells were transduced the next day with 1000 ng of plasmid DNA plus 250 ng pMD2.G (Cat# 12259, Addgene) and 750 ng psPax2 (Cat# 12260 ...
-
bioRxiv - Cell Biology 2021Quote: ... gonads of young adult BOX428 animals were microinjected with 30 ng/μl Pelt-2::αGFP-NB::ZIF-1 and 2.5 ng/μl Pmyo-2::GFP (#Addgene 26347) as a co-injection marker ...
-
bioRxiv - Cell Biology 2023Quote: ... supplemented with 2.5ul PLUS reagent with 1000 ng of reporter donor plasmid and 500 ng of each TALEN-L (Addgene 35431) and TALEN-R (Addgene 35432 ...
-
bioRxiv - Developmental Biology 2023Quote: Transgenic animals were generated by injecting transgenes (15 ng μl-1 each) mixed with a co-injection marker pRF-4 (120 ng μl-1) (Addgene) expressing a dominant negative variant of the rol-6 gene and an empty vector pSK (up to 200 ng μl-1 of DNA ...
-
bioRxiv - Genomics 2020Quote: ... The ORI minimal promoter and flanking region was PCR amplified from the hSTARR-seq plasmid (Addgene #99296) with a custom primer that included homology for Gibson cloning and KAPA HiFi HotStart ReadyMix (Kapa KK2602 ...
-
bioRxiv - Genomics 2020Quote: ... The resulting DNA fragments were cloned into the linearized STARR-seq vector (Addgene #71509, AgeI-SalI digested) using the In-Fusion HD kit (Takara) ...
-
bioRxiv - Cancer Biology 2021Quote: ... 500 ng of pMD2.G (Addgene plasmid #12259), and 20 µL of Lipofectamine 2000 (ThermoFisher ...
-
bioRxiv - Cell Biology 2020Quote: ... luciferase plasmids plus 10 ng of Renilla (Addgene) luciferase plasmids ...
-
bioRxiv - Developmental Biology 2020Quote: ... Using 1 ng pCFD6 (Addgene, Cat. No: 73915) as template ...
-
bioRxiv - Developmental Biology 2019Quote: ... 60 ng/μl pJW1285 (61252; Addgene; Ward, 2015), and 2.5 ng/μl Pmyo-2::tdTomato as a co-injection marker ...
-
bioRxiv - Cancer Biology 2020Quote: ... 500 ng of pMD2.G (Addgene plasmid #12259), and 20 μL of Lipofectamine 2000 (ThermoFisher ...
-
bioRxiv - Molecular Biology 2020Quote: ... and pHsp70-Cas9 (52) (250 ng/μl) (Addgene plasmid # 60944 ...
-
bioRxiv - Developmental Biology 2019Quote: ... 50 ng/µl Peft-3::Cas9 (Addgene 46168) and 2,5 ng/µl Pmyo-2::tdTomato ...
-
bioRxiv - Bioengineering 2020Quote: ... 500 ng pegRNA plasmid (cloned into Addgene #132777), and 200 ng secondary nicking sgRNA plasmid ...
-
bioRxiv - Microbiology 2021Quote: ... 100 ng of plasmid plRL19 (Addgene plasmid #163634) was also added ...
-
bioRxiv - Neuroscience 2020Quote: ... pCAG-Flpo (Addgene #125576, 3-10 ng/μL) and pCAFNF-tdTomato (Addgene#125575 ...
-
bioRxiv - Systems Biology 2021Quote: ... 750 ng of pCMV delta R8.2 (Addgene #12263) and 562.5 ng of an individual vector was added to 125 μl of Opti-MEM ...
-
bioRxiv - Systems Biology 2021Quote: ... Separately 187.5 ng of pMD2.G (Addgene #12259), 750 ng of pCMV delta R8.2 (Addgene #12263 ...
-
bioRxiv - Microbiology 2021Quote: ... mixed with 600 ng of PsPax2 (Addgene # 12260), 600 ng of the lentiviral transfer vector pLenti_CMV-EGFP-2A-mNeonGreen (Addgene # 171599) ...
-
bioRxiv - Biochemistry 2021Quote: ... and 250 ng of pMD2.G (Addgene #12259) using FuGENE 6 per manufacturers protocols ...
-
bioRxiv - Cell Biology 2020Quote: ... 500 ng Cas9 expression plasmid (Addgene plasmid #43945), and 200 ng of pMaxGFP via nucleofection (Lonza ...
-
bioRxiv - Molecular Biology 2022Quote: ... 250 ng of pcDNA1-TatHIV (Addgene plasmid #138478) and 500 ng of pMD2.G (VSV-G ...
-
bioRxiv - Microbiology 2022Quote: ... 100 ng of plasmid plRL19 (Addgene plasmid #163634) was also added ...
-
bioRxiv - Microbiology 2023Quote: ... 500 ng of psPAX2 packing vector (Addgene #12260), 333 ng of the pMD2.G VSV-G expression vector (Addgene #12259) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 200 ng of pCMV-Tol2 (Addgene no. 31823) and 50 ng CMV-GFP ...
-
bioRxiv - Physiology 2024Quote: ... 200 ng pDisplay-gGRAB5HT2m-IRES-mCherryCAAX (Addgene, #208710), 200 ng pcDNA3-5-HT2A-P2A-GCaMP8m ...
-
bioRxiv - Molecular Biology 2024Quote: ... 100 ng of the EPAC sensor (Addgene #61622) were mixed with 0.2 µL of lipofectamine 2000 following the transfection method previously described ...
-
bioRxiv - Biochemistry 2023Quote: ... and 500 ng of pMD2.G (Addgene #12259) using Lipofectamine 3000 (Invitrogen ...
-
bioRxiv - Synthetic Biology 2024Quote: ... 375 ng of PE4max enzyme plasmid (Addgene #174828), 62.5 ng of crRNA plasmid ...
-
bioRxiv - Cell Biology 2021Quote: ... and transfected with 100 ng of the indicated ORF6 expression construct along with either 50 ng of an ER marker (eGFP-Calnexin; Addgene: 57122), 50 ng of a Golgi marker [mTag-β-galactosidase (1-61)] ...
-
bioRxiv - Neuroscience 2021Quote: HEK293 cells were transfected with 500 ng of equimolar pooled SCN1A_h1b sgRNAs (Table 4) and 500 ng dCas9p300Core (Addgene, plasmid #61357) using Lipofectamine 3000 ...
-
bioRxiv - Synthetic Biology 2020Quote: ... The integration of the reporter was performed by co-transfecting 1000 ng TagRFP reporter (5xTetO-pEF-TagRFP-3xNLS) donor plasmid and 500 ng of each TALEN arm (AAVS1-TALEN-L (Addgene #35431) targeting TGTCCCCTCCACCCCACA and AAVS1-TALEN-R (Addgene #35432 ...
-
bioRxiv - Systems Biology 2022Quote: ... into the AAVS1 locus by electroporation of K562 cells with 1000 ng of reporter donor plasmid and 500 ng of each TALEN-L (Addgene #35431) and TALEN-R (Addgene #35432 ...
-
bioRxiv - Synthetic Biology 2022Quote: ... into the AAVS1 locus by electroporation of K562 cells with 1000 ng of reporter donor plasmid and 500 ng of each TALEN-L (Addgene #35431) and TALEN-R (Addgene #35432 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Transfer lentiviral plasmids (2500 ng) were diluted in 200 µl serum-free medium together with 225 ng pCMV-VSV-G (Addgene #8454) and 2250 ng psPAX2 (Addgene #12260 ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The well was co-transfected with 250 ng of a level 3 vector containing a construct of interest and 125 ng of BxB1 expression plasmid (Addgene #51271) using JetPrime (VWR) ...
-
bioRxiv - Molecular Biology 2024Quote: ... reporter cell lines were generated by TALEN-mediated homology-directed repair to integrate enhancer reporter donor constructs into the AAVS1 locus by electroporation of 1 × 106 K562 cells (or K562 cells selected to have the desired synthetic transcription factor) with 1 ng of reporter donor plasmid and 0.5 ng of each TALEN-L (Addgene no. 35431) and TALEN-R (Addgene no ...
-
Comparative performance of the BGI and Illumina sequencing technology for single-cell RNA-sequencingbioRxiv - Genomics 2019Quote: Comprised of cultured human trabecular meshwork cells (TMWCs) that had been transfected with a CROP-seq (Addgene: 99248) guide RNA (gRNA ...