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Citations for Addgene :
1 - 50 of 472 citations for NGS ChIP Seq kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: Genetic perturbations in snARC-seq reply on the CROP-seq vector (Addgene, pBA950)84 ...
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bioRxiv - Microbiology 2021Quote: ... CROP-seq-opti (Addgene # 106280) was linearized by BsmBI (New England Biolabs # R0580S ...
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bioRxiv - Genomics 2019Quote: The CROP-seq plasmid1 (Addgene 86708) was digested (SnaBI and NsiI ...
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bioRxiv - Genomics 2023Quote: The STARR-seq vectors (Addgene #99296) were linearized by restriction enzyme digestion using 2μl AgeI-HF (NEB R3552S ...
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bioRxiv - Cancer Biology 2022Quote: ... The modified CROP-seq-MS2 plasmid was obtained as following: the lentiviral CROP-seq-opti vector (Addgene plasmid #106280 was a gift from Jay Shendure ...
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bioRxiv - Genomics 2019Quote: ... The CROP-seq-opti vector (Addgene #106280) was used for sgRNA expression ...
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bioRxiv - Molecular Biology 2022Quote: ... The CROP-seq-opti plasmid (Addgene, #106280) was digested with BsmBI and purified from agarose gel using QIAquick Gel Extraction Kit (QIAGEN) ...
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bioRxiv - Pathology 2020Quote: ... the lentiviral vector used was pLV-(shRNA)-mCherry:T2A:Puro-U6 (Nck1 target seq: GGGTTCTCTGTCAGAGAAA; Nck2 target seq: CTTAAAGCGTCAGGGAAGA; VectorBuillder) with 3rd generation lenti components provided from Addgene; pMD2.G (12259) ...
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bioRxiv - Molecular Biology 2019Quote: ... The lentiviral vectors included pLV-(shRNA)-mCherry:T2A:Puro-U6 for Nck1 (target seq: GGGTTCTCTGTCAGAGAAA) and Nck2 (target seq: CTTAAAGCGTCAGGGAAGA) with 3rd generation lenti components provided from Addgene; pMD2.G (12259) ...
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bioRxiv - Genomics 2021Quote: ... AVA-Seq plasmids are readily available from Addgene.org ...
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bioRxiv - Cancer Biology 2019Quote: The CROP-seq-Guide-Puro vector (Addgene, #86708) was modified by replacing the puromycin resistance gene with BFP ...
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bioRxiv - Molecular Biology 2020Quote: ... was synthesized by GENEWIZ and cloned into CROP-seq vector (Addgene, #86708) at BsmBI sites ...
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bioRxiv - Biophysics 2022Quote: ... This resulted in the plasmid pASK-IBA5-chiP which was further used as the template for cloning the chiP gene into pET19b plasmid (Addgene). This was done using the primer pairs pET19b-chiP and chiP-pET19b with the Gibson cloning method (Table S2) ...
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bioRxiv - Genomics 2023Quote: ... Oligonucleotides containing these sequences and flanked with adapters with homology to a CROP-seq vector that we have previously altered23 to contain a CRIPSRi optimized guide RNA backbone79 (CROP-seq-OPTI, Addgene 106280) were synthesized individually for experiments related to Fig ...
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bioRxiv - Genetics 2020Quote: ... cloned these gRNAs into Crop-seq-opti (Addgene #106280), and transduced THP1 cells at a multiplicity of infection of 0.3 to ensure most cells contained 1 gRNA integration.
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bioRxiv - Genomics 2020Quote: ... We digested the STARR-Seq ORI vector (Addgene 99296) with AgeI and SalI restriction enzymes (NEB) ...
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bioRxiv - Genomics 2023Quote: ... The second generation hSTARR-seq ORI plasmid (Addgene: #99296) was digested with Sall-HF and Agel-HF restriction enzymes ...
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bioRxiv - Cancer Biology 2022Quote: ... The pBA904 Perturb-seq sgRNA vector backbone (Addgene, #122238) was digested using FastDigest BstX1 and Blp1 (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... and 83 ng of nicking guide RNA-(ngRNA-) encoding plasmid or 900 ng of NG-ABE-encoding plasmid (NG-ABE8e, Addgene #138491) and 250 ng of sgRNA-encoding plasmid with the following parameters ...
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bioRxiv - Cell Biology 2023Quote: ... Transfection was performed using 1100 ng of total DNA (500 ng transfer plasmid, 500 ng pCMVR8.74 Addgene plasmid # 22036 ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR-amplified Cas9n-NG DNA from pX330-SpCas9-NG (Addgene# 117919), PCR-amplified UGI DNA from pLenti-TRE3G-FNLS-PGK-Puro (Addgene# 110847 ...
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bioRxiv - Genomics 2020Quote: ... CROP-seq-opti was a gift from Jay Shendure (Addgene #106280)35 ...
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bioRxiv - Genomics 2022Quote: Human STARR-seq-ORI vector was obtained from Addgene (plasmid #99296). 8ug of STARR-seq plasmid was digested with 20uL AgeI-HF® (R3552 ...
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bioRxiv - Molecular Biology 2020Quote: ... Cas9-expressing HAP1 cells were transduced with CROP-seq (Addgene, #86708) virus expressing following gRNAs ...
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bioRxiv - Genomics 2019Quote: ... while adding homology to the STARR-seq vector (Addgene ID:71509)12 on the 5’ end and a spacer sequence on the 3’ end that we use for all subsequent libraries ...
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bioRxiv - Microbiology 2024Quote: Libraries were cloned into a CROP-seq-puro-v2 (Addgene #127458) backbone and lentivirus was then produced and transduced as previously described (Feldman et al. ...
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bioRxiv - Systems Biology 2019Quote: ... 1000 ng of lentiCRISPRv2_sgMS2_1 and 300 ng pCAG-mTagBFP2 (Addgene, Plasmid #122373) plasmids were transfected using Lipofectamine 3000 into each cell line ...
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bioRxiv - Immunology 2021Quote: ... NG-ABEmax (35) (Addgene plasmid #112095 ...
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bioRxiv - Immunology 2022Quote: ... 750 ng pSPAX2 (Addgene), 250 ng VSVG ...
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bioRxiv - Cell Biology 2021Quote: ... The sgRNA (100 ng/μl) and SEC repair template (20 ng/μl) plasmids combined with Peft-3::Cas9 (60 ng/μl; Addgene #46168) and Pmyo-2::mCherry co-injection marker (2.5 ng/μl ...
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bioRxiv - Cell Biology 2023Quote: ... Transfection was performed using 1100 ng of total DNA (500 ng transfer plasmid, 500 ng pCMVR8.74 Addgene plasmid # 22036, 100 ng pCMV-VSV-G Addgene plasmid # 8454) The day before transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 ng of CB1-GFP and 100 ng of Rab5-BFP (Addgene #49147) were mixed with 0.2 µL of lipofectamine 2000 following transfection method previously described then added to the wells ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 ng of CB1-GFP and 100 ng of R-GECO (Addgene #32444) were mixed with 0.2 µL of lipofectamine 2000 transfection reagent ...
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bioRxiv - Cancer Biology 2023Quote: ... NLS-CHIP was generated by replacing EBFP from pEBFP-nuc (Addgene, 14893) with CHIP insert excised from pIRES-CHIP.
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bioRxiv - Bioengineering 2021Quote: ... 5 uL of serum-free media was added to 200 ng of plasmid DNA (100 ng CAG-nanobody-TagBFP plasmid and 100 ng CAG-dsRed plasmid (Addgene plasmid 11151) (Matsuda and Cepko ...
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bioRxiv - Bioengineering 2021Quote: ... with 1000 ng of reporter and 500 ng of each TALEN-L (Addgene #35431) and TALEN-R (Addgene #35432 ...
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bioRxiv - Genomics 2022Quote: ... The SpCas9-NG ORF was subcloned from pX330-SpCas9-NG obtained from Addgene (#117919), and assembled with TEF1 promoter and CYC1 terminator (Zhang et al. ...
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bioRxiv - Neuroscience 2023Quote: ... PCDNA3-FlipGFP (Casp3 cleavage seq) T2A mCherry obtained from Addgene (Catalogue ID 124428). Cobalt(II ...
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bioRxiv - Microbiology 2024Quote: ... The STARR-seq luciferase reporter (vector_ORI_empty) was a gift from Alexander Stark (Addgene plasmid # 99297 ...
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bioRxiv - Genomics 2020Quote: ... and NG-ABEmax (Addgene #124163) were gifts from David Liu ...
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bioRxiv - Microbiology 2022Quote: ... 400 ng psPAX2 (Addgene 12260), and 500 ng plentiguide puro expression vector (Addgene cat # 52963) ...
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bioRxiv - Cell Biology 2021Quote: ... psPAX2 (Addgene, 12260, 1,250 ng), and the sgRNA containing vector (e.g. ...
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bioRxiv - Genomics 2021Quote: ... psPAX2 (Addgene 12260, 1250 ng)62 ...
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bioRxiv - Cancer Biology 2021Quote: ... 2250 ng psPAX2 (Addgene 12260) and 225 ng VSV-G (Addgene 8454 ...
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bioRxiv - Biochemistry 2021Quote: ... 750 ng psPAX2 (Addgene #12260), and 250 ng of pMD2.G (Addgene #12259 ...
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bioRxiv - Genetics 2020Quote: ... psPAX2 (Addgene 12260, 1250 ng), and the transfer vector (e.g. ...
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bioRxiv - Molecular Biology 2022Quote: ... 500 ng psPAX2 (Addgene #12260), 50 ng pMD2.G (Addgene #12259 ...
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bioRxiv - Genomics 2023Quote: ... psPAX2 (Addgene 12260, 1250 ng), and the transfer vector (e.g. ...
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bioRxiv - Biochemistry 2023Quote: ... 900 ng psPAX (Addgene, #12260), and 100 ng pMD2.G (Addgene ...
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bioRxiv - Cell Biology 2023Quote: ... 600 ng psPAX2 (Addgene, 12260), and 300 ng pMD2 (Addgene ...