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Citations for Addgene :
501 - 550 of 2724 citations for Mouse Macrophage expressed gene 1 protein MPEG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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A CRISPR activation screen identifies FBXO22 as an E3 ligase supporting targeted protein degradationbioRxiv - Biochemistry 2023Quote: ... FKBP12-EGFP construct was generated by cloning FKBP12 gene into a modified Artichoke plasmid (Addgene plasmid#: 73320) in which the selection marker puromycin was replaced with neomycin ...
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bioRxiv - Microbiology 2023Quote: Full length WT gp210 and mutant genes were cloned into UC Berkeley Macrolab vector 1B (Addgene #29653) to generate N-terminal fusions to a TEV protease-cleavable His6-tag ...
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bioRxiv - Cell Biology 2024Quote: ... CAPS-FLAG was created by Gateway cloning the CAPS gene into pDEST-N-terminal FLAG (Addgene #18700), providing expression of N-terminally FLAG-tagged CAPS control of the cmv-promoter ...
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bioRxiv - Cell Biology 2024Quote: ... CAPS-gfp was created by Gateway cloning the CAPS gene into pDEST-CMV-C-EGFP (Addgene #122844), to create an C-terminally-tagged gfp construct under control of the cmv promoter.
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bioRxiv - Neuroscience 2024Quote: ... was injected with a mixture of Cre-expressing and Cre-dependent adeno-associated viral vectors carrying the genes for ChR2 and tdTomato (AAV9.CamKII.4.Cre.SV40 and AAV9.CAG.Flex.ChR2.tdTomato, Addgene). Following a post-injection survival period of 9 weeks ...
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bioRxiv - Genomics 2020Quote: The CRISPR/Cas9 plasmid (CTCF-mouse-3sgRNA-CRISPRexp-AID) was assembled using the Multiplex CRISPR/Cas9 Assembly System kit57 (a gift from Takashi Yamamoto, Addgene kit #1000000055). Oligonucleotides for three gRNA templates were synthesized ...
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bioRxiv - Cancer Biology 2021Quote: Mouse Trp53 transcript variant 1 (NM_011640.3) was cloned into the retroviral vector pMSCV-IRES-GFP II (Addgene plasmid #52107). Trp53 point mutations were introduced using site-directed mutagenesis with the Q5 Site-Directed Mutagenesis Kit (New England Biolabs E0552S) ...
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bioRxiv - Neuroscience 2020Quote: ... we infected adult Brn3cCre mouse retinas with 1 ml Cre dependent AAV Virus (AAV2-CAG-FLEX-EGFP-WPRE, Addgene catalog # ...
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bioRxiv - Cell Biology 2024Quote: ... shRNAs targeting luciferase or mouse Nr1h3 and Rara were cloned into the pLKO.1 vector (Addgene, MA, USA, #8453). The lentiviral vectors were co-transfected with the packaging vectors pCMV-deltaR8 (Addgene ...
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bioRxiv - Neuroscience 2022Quote: ... we cloned the dgn-1 promoter and AcNPV p10 3’UTR into the vector pPD49.26 (from the Andrew Fire plasmid kit, Addgene Kit #1000000001) to generate plasmid pNTC2 ...
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bioRxiv - Biochemistry 2022Quote: ... full-length FUS with an N-terminal histidine tag and maltose-binding protein fusion (pTHMT FUS 1-526, AddGene #98651), and FUS RGG3 with N-terminal histidine tag and MBP fusion (pTHMT FUS RGG3 (453-507) ...
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bioRxiv - Neuroscience 2021Quote: ... or KH1*KH2* mutants were PCR amplified from their respective pAc5.1B-EGFP vector and cloned into the HindIII and EcoRI sites of the pAc5.1-lambdaN-HA vector (a gift from Elisa Izaurralde; Addgene plasmid #21302) (Behm-Ansmant et al. ...
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bioRxiv - Neuroscience 2021Quote: ... was PCR amplified and cloned downstream of EGFP in the pAc5.1B-EGFP vector (a gift from Elisa Izaurralde; Addgene plasmid #21181) using the HindIII and EcoRI restriction sites to make pAcB5.1-EGFP-FMRP ...
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Hippocampal efferents to retrosplenial cortex and lateral septum are required for memory acquisitionbioRxiv - Neuroscience 2020Quote: ... enhanced yellow fluorescent protein (eYFP) or enhanced green fluorescent protein (eGFP): AAV1.CaMKIIa.eNpHR3.0.eYFP.WPRE.hGH (Addgene, #26971), AAV1.CaMKII.eYFP ...
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bioRxiv - Synthetic Biology 2022Quote: A plasmid construct containing only the maturation protein and his-tagged coat protein dimer (Addgene # 179156) was transformed into Rosetta2™ (DE3 ...
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bioRxiv - Immunology 2021Quote: ... a previously described lentiviral expression plasmid carrying RFP reporter and puromycin resistance genes (pL-SFFV.Reporter.RFP657.PAC, Addgene#61395) was used with the following modifications ...
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bioRxiv - Synthetic Biology 2020Quote: Cloning of pGuides into piggybac transposon vectors: A piggybac transposon vector harboring a puromycin resistance gene (Addgene, #104537) was digested in reactions containing AgeI ...
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bioRxiv - Immunology 2021Quote: Gene knock-outs were generated using either the lentiCRISPRv2 system (Addgene plasmid #52961, a gift from Feng Zhang)40 using the methods described above and specific sgRNA-encoding oligos (Supplementary Dataset 4) ...
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bioRxiv - Cell Biology 2020Quote: ... the pRho plasmid driving gene expression under the 2.2 kb bovine rhodopsin promoter was used (Addgene, plasmid # 11156). For Xenopus transgenics ...
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bioRxiv - Cell Biology 2022Quote: ... the guide RNA targeting exon 49 of the MUC2 gene was cloned into the pSPgRNA plasmid (Addgene #47108) using the following oligonucleotide sequences ...
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bioRxiv - Cancer Biology 2022Quote: ... sgRNAs or sgRNA1-scaffold-bovineU6-sgRNA2 cassettes (synthesized as gene-blocks by IDT) were cloned into BsmBI-digested LRG2.1_Neo (Addgene_125593). Sequences of sgRNAs are provided in Supplementary Table S3 ...
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bioRxiv - Cancer Biology 2021Quote: The genome-wide Brie CRISPR-KO library (4 sgRNAs per gene; ~ 80.000 sgRNAs) was purchased from Addgene (#73632) and amplified according to the supplier’s protocol ...
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bioRxiv - Immunology 2020Quote: ... The vector pEQ276 (encoding CMV IE1 and 2 genes) was a gift from Adam Geballe (Addgene plasmid #83945)67 ...
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bioRxiv - Developmental Biology 2022Quote: The rat Ocm gene (sequence in Figure S01) was cloned into pcDNA3.1CFP and pEGFP-N1 FLAG (Addgene #60360). Then ...
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bioRxiv - Microbiology 2022Quote: ... The target sequence for the ACE2 gene (5′-TGCTGCTCAGTCCACCATTG-3′) was designed using CRISPR direct (https://crispr.dbcls.jp) and cloned into plentiCRISPR plasmids (21) (Addgene plasmid #52961 ...
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bioRxiv - Biochemistry 2021Quote: SARS-CoV-2 S-Pseudotyped lentivirus27,43 were produced by co-transfection of HEK-293T cells with plasmids bearing a GFP reporter gene (pLB was a gift from Stephan Kissler, Addgene plasmid #11619; http://n2t.net/addgene:11619; RRID:Addgene_11619), a lentivirus backbone (VRC5602 ...
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bioRxiv - Microbiology 2021Quote: ... TBK1 and KHNYN genes were cloned into BsmBI restriction enzyme sites in the lentiviral CRISPR plasmid lentiCRISPRv2 (Addgene). LentiCRISPR VLPs were produced on HEK293T cells seeded on a 10cm dish ...
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bioRxiv - Systems Biology 2020Quote: The catI gene was cloned from pTKIP-cat which was a gift from Edward Cox & Thomas Kuhlman (Addgene plasmid # 41065 ...
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bioRxiv - Molecular Biology 2021Quote: ... and miRFP2 genes were PCR amplified and swapped with mClover2 in pmClover2-tubulin-C-18 plasmid (Addgene #56376) and with TagRFP675 in pTagRFP675-actin-C1 (Addgene #44277 ...
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bioRxiv - Biochemistry 2022Quote: ... The wild-type 14-3-3 ζ gene was cloned into NcoI/XhoI digested pBAD plasmid (Addgene #85482) with a TEV cleavable C-terminal His6 purification tag ...
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bioRxiv - Microbiology 2022Quote: ... expressing 76,441 sgRNAs against 19,114 human genes + 1,000 non-targeting sgRNA controls in plentiCRISPRv2 was obtained from Addgene (51). The library was electroporated into Endura electrocompetent cells (# 60242 ...
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bioRxiv - Molecular Biology 2022Quote: All engineered SARS-CoV-2 S variant ectodomains were designed based on the HexaPro gene (Addgene plasmid #154754) containing stabilizing proline mutations at positions F817P ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The hACE-P2A-Puro gene encoding VSV-G lentiviral particles were produced by second generation lentiviral plasmids (Addgene) and transfected both pLenti-hACE-P2A-Puro ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The gene construct was inserted into the vector backbone of pBAC-ECFP-15xQUAS_TATA-mcd8-GFP-SV40 (Addgene 104878) and the 3xP3 ECFP integration reporter was replaced by 3xP3 DsRed (Matz et al. ...
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bioRxiv - Cell Biology 2023Quote: ... pMyo-mCherry was prepared by cloning the myoglobin gene from Physeter catodon (Sperm Whale, Addgene Plasmid pMB413a, #20058) into the pmCherry N1 vector (Clonetech ...
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bioRxiv - Genetics 2023Quote: ... fused to the herpes simplex virus thymidine kinase-encoding gene (HSV-TK) from the pFA6a-HyTkAX vector (Addgene plasmid # 73898; http://n2t.net/addgene:73898; RRID:Addgene_73898) [80] ...
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bioRxiv - Cell Biology 2023Quote: ... The CRY2olig cDNA was prepared through site-directed mutagenesis (E409G) of CRY2 gene that was obtained from Addgene. The PCR products were subcloned into pcDNA3.1 myc/His ver ...
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bioRxiv - Microbiology 2024Quote: ... The viral gene constructs used in this study were sourced as follows: pGwf and phGf were from Addgene; pALTOw ...
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bioRxiv - Biophysics 2024Quote: ... and 15 µg lat-egfp-p2a-nfat-mcherry bicistronic gene in the MCSV backbone (similar to AddGene #91975) were pre-mixed in 1 mL of Opti-MEM media (Life Technologies Cat #:51985-034 ...
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bioRxiv - Microbiology 2024Quote: Gene deletions for BtΔsusCD and BoΔsusCD mutants were generated using counterselectable allelic exchange with pSIE1 (Addgene plasmid #136355) as described previously24 ...
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bioRxiv - Neuroscience 2024Quote: ... AAV-DJ-hSYN1::mTurquoise2 (Produced by Stanford University Neuroscience Gene Vector and Virus Core using Addgene, plasmid #99125), AAV-DJ-hSYN1::mCherry (Stanford University Neuroscience Gene Vector and Virus Core ...
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bioRxiv - Microbiology 2024Quote: ... sequences targeting the human DDX5 gene were designed using the CRISPOR tool (http://crispor.tefor.net) and cloned into the pX459-V2 vector (Addgene Plasmid #62988 was a gift from Feng Zhang (30)) ...
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bioRxiv - Biochemistry 2024Quote: ... and T7 Ocr (NCBI Accession # NP_041954.1) genes were cloned into UC Berkeley Macrolab vectors 2-BT (Addgene #29666) and 13S-A (Addgene #48323 ...
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bioRxiv - Genomics 2019Quote: ... we cloned effector proteins (PguCas13b: Addgene 103861 ...
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bioRxiv - Genetics 2019Quote: ... Cas9 protein and mRNA (44758; Addgene) were co-microinjected into zygotes [F1 hybrids between strains FVB/NJ and B6(Cg)-Tyrc-2J/J] using the reagent concentrations listed in Table S1 ...
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bioRxiv - Bioengineering 2021Quote: ... the Spike protein (Addgene plasmid # 145032) was cloned downstream of the TRE3G promoter ...
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bioRxiv - Developmental Biology 2020Quote: ... a FLAG-tag version of the codon-optimized mouse DUX was amplified by PCR (Primers in Extended Table 1) from pCW57.1-mDUX-CA (Addgene 99284) and subcloned into the pBS31 plasmid (pBS31-FLAG_mDUX) ...
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bioRxiv - Neuroscience 2020Quote: Cortical pyramidal neurons (CPN)s in WT and YAC128 cultures were labeled by transfecting a subset of neurons (1 of 2.7 million) with a cytoplasmic green fluorescent protein (GFP) (Addgene plasmid 37825) at the time of platting ...
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bioRxiv - Cell Biology 2022Quote: ... The Capping Protein (F-actin-capping protein subunit alpha-1 and F-actin-capping protein subunit beta) plasmid was a gift from Antoine Jégou & Guillaume Romet-Lemonne (Addgene plasmid #89950).
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bioRxiv - Cell Biology 2022Quote: ... reporter under the constitutive elongation factor 1-α (EF1α) promoter and upstream of a P2A linker followed by the tdTomato fluorescent protein (gift from Kazuhiro Oka;Addgene plasmid #72486 ...