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Citations for Addgene :
501 - 550 of 1989 citations for 2' 3' cGAMP STING based FRET Detection Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: Toronto human knockout pooled library (TKOv3) containing 71,090 based on a lentiCRISPRv2 backbone was a gift from Jason Moffat (Addgene #90294). The library was transformed and amplified using 25 μl Endura Competent Cells (Lucigen ...
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bioRxiv - Cell Biology 2022Quote: ... the retroviral FLAG-5SA-YAP and FLAG-5SA-S94A-YAP plasmids were generated via restriction-based cloning from pQCXIH-Myc-YAP-5SA (Addgene plasmid #33093 36 and pCMV-Flag-YAP-5SA/S94A (Addgene plasmid #33103 42 ...
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bioRxiv - Cell Biology 2022Quote: ... the retroviral FLAG-5SA-YAP and FLAG-5SA-S94A-YAP plasmids were generated via restriction-based cloning from pQCXIH-Myc-YAP-5SA (Addgene plasmid #33093 36 and pCMV-Flag-YAP-5SA/S94A (Addgene plasmid #33103 42 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The vectors used for CRISPR/Cas9 were based on the pML104 vector (a gift from John Wyrick; Addgene plasmid # 676380), into which the guide sequences were introduced by site-directed mutagenesis ...
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bioRxiv - Cancer Biology 2023Quote: ... a custom PiggyBac CRISPR gRNA expression plasmid based on the lentiviral construct pCRISPRia (a gift from Jonathan Weissman, RRID:Addgene 84832). Complementary gRNA-containing oligos were hybridized and phosphorylated in a single reaction ...
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bioRxiv - Neuroscience 2023Quote: ... The AAVS1-targeting TALENs are based on hAAVS1 1R TALEN and hAAVS1 1L TALEN (plasmid nos. 35432 and 35431, respectively; Addgene) and were generated by golden gate assembly ...
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bioRxiv - Genetics 2020Quote: A pair of TALENs targeting zebrafish tnfaip3 (A20) exon 2 were generated using “PLATINUM Gate TALEN Kit” (Addgene, #1000000043). 0.2 ng of mRNA encoding the TALEN pair was delivered into the cytoplasm of wild-type zebrafish embryos at the one-cell stage ...
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bioRxiv - Bioengineering 2022Quote: ... with 2 μg MLC-2 plasmid (pEGFP-MRLC1, Addgene, #35680), 10 μg RAR-β siRNA (Santa Cruz ...
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bioRxiv - Neuroscience 2023Quote: ... The 5-HT1eR and 5-HT1FR PRESTO-Tango constructs were obtained from Addgene. For transfection ...
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bioRxiv - Neuroscience 2023Quote: ... virus for optogenetic inhibition or excitation of preBötCOT-R neurons in OT-R::Cre mice was obtained from Addgene (Table 2, Addgene viral prep # 154951 ...
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bioRxiv - Neuroscience 2023Quote: ... The AAV1-phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE virus for tracing experiments from preBötCOT-R neurons to nACardiac neurons in OT-R::Cre mice was obtained from Addgene (Table 2, Addgene viral prep # 51509 ...
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bioRxiv - Cell Biology 2020Quote: ... 25 ng/µl of co-injection marker pCFJ104 (Pmyo-3:mCherry:unc-54 3’UTR, a gift from Erik Jorgensen, Addgene plasmid #19328 ...
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bioRxiv - Neuroscience 2022Quote: ... A pcDNA3 flag HA 14-3-3 β plasmid was a gift from William Sellers (Addgene plasmid # 8999; http://n2t.net/addgene:8999; RRID:Addgene_8999). All plasmids were verified by Sanger sequencing and/or long-read sequencing (Iowa IIHG Genomics core or Plasmidsaurus ...
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bioRxiv - Molecular Biology 2024Quote: ... SK-BR-3 HER2-knockout (SK-BR-3 KO) were obtained using pSpCas9 BB-2A-Puro (PX459) V2.0 (9200 bp, Addgene) containing a sgRNA sequence (5’-TCATCGCTCACAACCAAGTG-3’ ...
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bioRxiv - Neuroscience 2024Quote: ... polyA_R, Table 3) and plasmid backbone (Col1E_F, Col1E_R, Table 4) were amplified from pPRISM-Stop-cmlc2-eGFP (Addgene kit #1000000154; Wierson et al., 2020). The PCR-amplified fragments were assembled using NEBuilder HiFi DNA Assembly Cloning Kit (E5520S ...
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bioRxiv - Developmental Biology 2021Quote: Reporter constructs were cloned into an expression vector based on the pBDP backbone (a gift from Gerald Rubin; Addgene plasmid #17566) as described in (36 ...
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bioRxiv - Cell Biology 2021Quote: ... EF1a-ERK-KTR-mScarlet lentiviral expression vector was generated by Gibson Assembly cloning based on an ERK-KTR-Clover plasmid from Markus Covert lab (Addgene #59150).
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bioRxiv - Cell Biology 2022Quote: The tagging strategy was based on a two-step tagging technique 45 using a tagging cassette based on the plasmid FLAG-LoxP-PURO-LoxP-HaloTERT WT HR Donor (Addgene # 86843), with the Halo-Tag exchanged by EGFP ...
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bioRxiv - Cancer Biology 2021Quote: Lentiviruses were made by Lipofectamine 2000-based co-transfection of 293FT cells with the respective lentiviral expression vectors and the packaging plasmids psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259 ...
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bioRxiv - Neuroscience 2019Quote: ... Reference sequences of the viral samples used in this study were based on deposited plasmids in Addgene: pSAD-F3-NPEST-iCRE-2A-mCherryPEST (Addgene #99608), pSAD-F3-NPEST-FLPo-2A-mCherryPEST (Addgene #99609) ...
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bioRxiv - Biochemistry 2022Quote: An insert of ZZUD and ZZUDL1340P were cloned out from a positive pGEX-6P-1-ZZUD or pGEX-6P-1 - ZZUDL1340P with PCR based cloning procedure into a pEGFP-C1 (Addgene 54759) or mRFP-C1 (Addgene 54764 ...
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bioRxiv - Neuroscience 2023Quote: ... NIR-GECO2G was expressed via AAV2.1-CAG-NIR-GECO2G-WPRE (AAV generated by Yale Vision Core based on Addgene plasmid #159605). For positive control experiments ...
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bioRxiv - Cell Biology 2023Quote: ... They were then cloned into a pCCC vector which is based on pSpCas9(BB)-2A-GFP vector (PX458, Addgene plasmid # 48138). The pCCC vector contains the complete U6 promoter for enhanced expression in hiPSCs ...
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bioRxiv - Neuroscience 2023Quote: ... For optogenetic activation of PRF/GlyT2+ cells we injected AAV5.EF1a.DIO.hChR2(H134R)-eYFP.WPRE.hGH (based on Addgene plasmid #20298, UNC Vector Core) or for control AAV5.EF1a.DIO.eYFP.WPRE.hGH (based on Addgene plasmid #27056 ...
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bioRxiv - Genetics 2023Quote: ... We generated SiT-ddCas12a-[Repr] by introducing the DNase-inactivating E993A by PCR-based mutagenesis using SiT-Cas12a-[Repr] (Addgene #133568) as template ...
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bioRxiv - Genomics 2023Quote: ... a custom PiggyBac CRISPR gRNA expression plasmid based on the lentiviral construct pCRISPRia (a gift from Jonathan Weissman, Addgene plasmid # 84832). Complementary gRNA-containing oligos were hybridized and phosphorylated in a single reaction ...
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bioRxiv - Synthetic Biology 2023Quote: ... Lentiviral particles containing the CD19 CAR element were produced by lipofectamine-based co-transfection of HEK293 cells with 3rd generation packaging plasmids pMD2.G (Addgene, #12259), pMDLg/pRRE (Addgene ...
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bioRxiv - Synthetic Biology 2023Quote: ... DNA constructs were generated via standard molecular biology techniques including overlap extension PCR and restriction site-based cloning and ligated directly into pENTR1a no ccdB (Addgene 17398) or the MMLV-based retroviral vector LZRS-Rfa (Addgene 31601) ...
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bioRxiv - Neuroscience 2024Quote: pAAV CAG-FLEx-FLPo was constructed by in-fusion-based PCR cloning utilizing the following two DNA fragments: i) SalI-AscI restriction fragment of pAAV CAG-FLEx-TCb (Addgene #48332) as a vector backbone ...
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bioRxiv - Cancer Biology 2024Quote: Lentiviruses were made by Lipofectamine 2000-based co-transfection of 293FT cells with the respective lentiviral expression vectors and the packaging plasmids psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259 ...
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bioRxiv - Molecular Biology 2024Quote: Plasmids for microinjection were generated by amplifying the coding and flanking sequences from MT7 genomic DNA and introducing them with the PCR-based method CPEC (Quan & Tian, 2011) into the L4440 plasmid (Addgene, USA). DevPF1 was expressed with its endogenous flanking regions (304 bp upstream of the DevPF1 start codon and 272 bp downstream of the DevPF1 stop codon) ...
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bioRxiv - Genomics 2024Quote: ... and the top two scoring sgRNAs for each target gene based on depletion or enrichment phenotypes were cloned into dual sgRNA lentivirus expression vectors with direct capture tags (Addgene 187241)40 using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs #E2621L ...
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bioRxiv - Cell Biology 2024Quote: The PI4P sensor based on optically-controlled phase separation was created by fusion of P4M domian from mCherry-P4M-SidM (#51471, Addgene, USA) to mGFP-CIBN ...
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bioRxiv - Neuroscience 2019Quote: ... forward primer-5’-AGCAGCACGACTTCTTCAAGTCC and reverse primer 5’-TGTAGTTGTACTCCAGCTTGTGC (modified protocol from Addgene, USA). A known concentration (2 × 109 molecules/µl ...
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bioRxiv - Microbiology 2023Quote: ... Truncation mutations in NL4-3 were created by subcloning the region of NL4-3 between EcoRI to XhoI into pBlueScript KS(-) (Addgene), followed by site-directed mutagenesis to introduce a stop codon to generate the desired truncation mutation ...
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bioRxiv - Neuroscience 2021Quote: ... pAAV-hSyn-Flpo-3×FLAG (no. 173047, Addgene), which has the hSyn promoter followed by Flpo with 3×FLAG C-terminal tag (OGS629 ...
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bioRxiv - Molecular Biology 2020Quote: ... 5ng of a reference plasmid (pNL4-3, Addgene) was spiked in to each DNA preparation and used for qPCR normalization (Table S1) ...
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bioRxiv - Developmental Biology 2019Quote: ... 50 ng/µl Peft-3::Cas9 (Addgene 46168) and 2,5 ng/µl Pmyo-2::tdTomato ...
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bioRxiv - Immunology 2021Quote: ... 3 μg of pIRES-eGFP plasmid DNA (Addgene) were digested with 250 U of Benzonase ...
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bioRxiv - Neuroscience 2020Quote: ... and 3 µg VSVG packaging vector (Addgene, 8454) in a 100 mm dish using a calcium phosphate transfection kit (CalPhos Mammalian Transfection kit ...
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bioRxiv - Bioengineering 2020Quote: ... 3 μg pCMV-VSV.G (Addgene, Watertown, MA; #8454), and 4 μg CD19-CAR-GFP transfer plasmid (Bloemberg et al ...
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bioRxiv - Neuroscience 2020Quote: ... pCAG-Flpo (Addgene #125576, 3-10 ng/μL) and pCAFNF-tdTomato (Addgene#125575 ...
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bioRxiv - Genetics 2020Quote: ... and pCFJ104 Pmyo-3-mCherry (Addgene plasmid 19328)(Frøkjær-Jensen et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... a p10 3’UTR fragment amplified from Addgene plasmid #100580 with primers 1010.C5 and 1010.C6 ...
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bioRxiv - Bioengineering 2020Quote: ... a p10 3’UTR fragment amplified from Addgene plasmid #100580 19 with primers 986.C3 and 986.C4 ...
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bioRxiv - Neuroscience 2023Quote: ... muscarinic receptor type 3/1 (CHRM3/1, Addgene) and GFP were co-expressed at a ratio of 8:4:3:1 ...
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bioRxiv - Neuroscience 2023Quote: ... and ipo13b sgRNA3 into pU6b:sgRNA#3 (Addgene #64247). Ligation reactions were carried out by mixing 1 µL 10x NEB CutSmart buffer ...
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bioRxiv - Synthetic Biology 2023Quote: ... pNO286-3 (a gift from Jeffrey Tabor, Addgene plasmid # 107746 ...
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bioRxiv - Cell Biology 2023Quote: ... pGEX-4T-3-mR7BD (Addgene #79149, Edinger Lab). To generate Emerald-Rab7L8A ...
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bioRxiv - Neuroscience 2023Quote: ... and 3) AAV9-hSyn-ChR2(H134R)-eYFP (RRID:Addgene_127090 ...