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Citations for Addgene :
351 - 400 of 1157 citations for Rat Phosphatidic Acid Phosphatase Type 2A PPAP2A ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... pSpCas9(BB)-2A-Puro (PX459) was a gift from Feng Zhang (Addgene plasmid # 62988) (33).
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Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistancebioRxiv - Cell Biology 2024Quote: Single guide RNA oligonucleotides were cloned into the pSpCas9-2A-GFP vector (Addgene #48138). The guide sequences were as follows ...
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bioRxiv - Cell Biology 2022Quote: Wild type (WT) VCP (Addgene #23971), A232E VCP (Addgene #23973) ...
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bioRxiv - Microbiology 2023Quote: ... and pBTK401 (Type 8) (Addgene_65109, Addgene_183127 ...
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bioRxiv - Neuroscience 2023Quote: Wild-type α-syn (Addgene, 3604628) was expressed and harvested from Escherichia coli as described previously29 ...
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bioRxiv - Cancer Biology 2021Quote: ... The Cas9 expression construct pSpCas9(BB)-2A-GFP was purchased from Addgene (Plasmid ID 44758). Stat1 (NM_001205313.1 ...
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bioRxiv - Molecular Biology 2021Quote: ... dsDNA guide inserts ligated into BbsI-digested pSpCas9(BB)-2A-GFP (PX458) (AddGene plasmid #48138), and plasmid verified by sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... and then insert it to the pLenti-EF1a-dCas9-VP64-2A-Blast backbone (Addgene 61425) to replace th dCas9-VP64 ...
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bioRxiv - Molecular Biology 2020Quote: ... pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene plasmid #62988; http://n2t.net/addgene:62988; RRID: Addgene_62988) was a gift from Feng Zhang ...
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bioRxiv - Molecular Biology 2021Quote: The Cas9 expression construct pSpCas9(BB)-2A-GFP was purchased from Addgene (Plasmid ID 44758). Stat1 (NM_001205313.1 ...
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bioRxiv - Bioengineering 2019Quote: We modified Cas9/gRNA expressing plasmid pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene plasmid #62988) by inserting the fragment coding the targeting gRNA using digestion/ligation protocol [15] ...
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bioRxiv - Developmental Biology 2019Quote: ... The guide RNA was cloned into pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene plasmid # 62988), as previously described (Ran et al. ...
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bioRxiv - Cancer Biology 2019Quote: SgRNAs targeting mouse Wnt5a or Yap1 were cloned into pSpCas9(BB)-2A-Puro (Addgene, #62988) and transfected into target cells ...
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bioRxiv - Cell Biology 2019Quote: ... 2 μg of pSpCas9(BB)-2A-GFP (PX458) (gift from Feng Zhang, Addgene plasmid #48138) containing the FOXJ1 targeting sgRNA sequence 5’-GGGCCTTCTTGTAAGAGGCC-3’ or the CCDC40 targeting sgRNA sequence 5’-CTCCTCGTTGGCGGCTGCGCAGG-3’ with 1 μg of MBX plasmid (gift from Linzhao Cheng ...
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bioRxiv - Cell Biology 2020Quote: ... and ligated into digested pspCas9(BB)-2A-puro plasmid (plasmid no. 62988, Addgene, Cambridge, MA). HEK293 cells were suspended in 2 ml of DMEM supplemented with 10% FBS and plated in 6-well plates ...
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bioRxiv - Genetics 2020Quote: ... were synthesized and cloned into doxycycline-inducible lentiviral pCW57-MCS1-2A-MCS2 vector (Addgene, #71782). THP1 TREM2 KO cells were transduced with lentiviral particles expressing either of wild-type TREM2 ...
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bioRxiv - Cancer Biology 2021Quote: ... as oligonucleotide DNA sequences and cloned into pSpCas9(BB)-2A-GFP (PX458) (Addgene; Cat#: 48138) to construct two active CRISPR vectors ...
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bioRxiv - Cancer Biology 2020Quote: ... spCas9-2A-EGFP (PX458) was a gift from Feng Zhang (Addgene plasmid #48138; RRID: Addgene_48138). Knockout cell lines were generated from mKate2-expressing A549 cells generated with a bleomycin-selectable lentiviral construct ...
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bioRxiv - Cancer Biology 2020Quote: ... spCas9-2A-EGFP (PX458) was a gift from Feng Zhang (Addgene plasmid #48138; RRID: Addgene_48138). Knockout cell lines were generated from mKate2-expressing A549 cells generated with a bleomycin-selectable lentiviral construct ...
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bioRxiv - Genomics 2022Quote: CRISPR targeting sequences were designed and cloned into pSpCas9(BB)-2A-Puro vector (Addgene #62988). Following transfection of K562 cells with Cas9 and sgRNA expressing plasmids for 24 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... These plasmids are based on all-in-one pSpCas9(BB)-2A-Puro (px459) (Addgene #62988) V2.0 and pSpCas9n(BB)-2A-Puro (px462 ...
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bioRxiv - Cell Biology 2022Quote: ... and ligated into the BbsI site of pSpCas9(BB)-2A-Puro vector (PX459, Addgene #62988). U-2 OS cells were transfected with two PX459 encoding sgRNAs using lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... sgRNAs were cloned into the pSpCas9(BB)–2A–GFP (PX458) vector (Addgene plasmid #48138 46) or PX330-Puro (kind gift from Prof Ciaran Morrison ...
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bioRxiv - Pathology 2019Quote: ... gRNA_ex93.0: 5’-GCGTGAGGACAACCGCGTGCAGG-3’) were cloned into the pSPCas9(BB)-2A-GFP plasmid (Addgene 48138) and introduced into CRL1502 iPSCs by reverse transfection using TransIT-LT1 (Mirus Bio) ...
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bioRxiv - Neuroscience 2020Quote: ... and were cloned into the pSpCas9(BB)-2A-Puro (PX459) V2.0 plasmid (Addgene plasmid 62988) according to a previously described protocol (Ran et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... We also generated knockout cells using pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene Plasmid #62988). Editing of the DPYSL2 locus in MDA-MB-231 cells was accomplished by either infecting cells with the “pLentiCRISPR” plasmid ...
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bioRxiv - Genomics 2021Quote: sgRNA were cloned in pSpCas9(BB)-2A-Puro (PX459) V2.0 (Feng Zhang laboratory, Addgene 62988). Briefly ...
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bioRxiv - Biochemistry 2020Quote: ... The guides RNAs were expressed from the plasmid pSpCas9(BB)-2A-GFP (pX458) (Addgene, #48138) (Ran et al. ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... with 20 mg Cas9 (pSpCas9(BB)-2A-Puro (PX459) V2.0 (gift from Feng Zhang; Addgene plasmid #62988 ...
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bioRxiv - Developmental Biology 2022Quote: ... The sgRNA/Cas9 plasmid was modified from SpCas9-2A-Puro V2.0 plasmid (Addgene, Feng Zang).
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bioRxiv - Neuroscience 2023Quote: ... 6.25 μg of Cas9 expression vector (pCAG-1BPNLS-Cas9-1BPNLS-2A-GFP; Addgene, plasmid 87109) and 12.5 μg of donor plasmid using Lipofectamine LTX (Thermo ...
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bioRxiv - Neuroscience 2022Quote: ... pXR001: EF1a-CasRX-2A-EGFP was a gift from Patrick Hsu (Addgene plasmid # 109049; RRID:Addgene_10904974). CasRX compatible guide RNA expression was achieved by cloning the guide sequences into the pXR003 vector backbone (pXR003 ...
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bioRxiv - Genetics 2023Quote: ... sgRNAs were cloned individually into the plasmid pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene, #62988) and then transfected into XEN cells using XfectTM transfection reagent (Clontech ...
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bioRxiv - Cancer Biology 2023Quote: pSpCas9(BB)-2A-GFP (px458) plasmid was a gift from Feng Zhang (Addgene plasmid #48138). The designated sgRNA sequences for each of the targeted genes were cloned into px458 using combinations of top and bottom oligonucleotides listed below.
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bioRxiv - Neuroscience 2023Quote: ... pSpCas9(BB)-2A-Puro (PX459) V2.0 was a gift from Feng Zhang (Addgene plasmid # 62988) was modified to target the region of interest ...
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bioRxiv - Neuroscience 2023Quote: Human iPSCs were edited using the pSpCas9(BB)-2A-GFP (PX458) construct backbone (Addgene #48138) described in Ran et al 201380 ...
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bioRxiv - Genetics 2023Quote: ... The guide was cloned into the pSpCas9(BB)-2A-Puro (PX459) V2.0 plasmid (Addgene, #62988) via restriction digestion and ligation ...
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bioRxiv - Cancer Biology 2023Quote: ... the Cas13d-NLS cassette was PCR-amplified from the pXR001_EF1a-CasRx-2A-EGFP (Addgene #109049) plasmid and cloned into pLX_TRC311-NLS-Cas13b-NES-P2A-Blast-eGFP ...
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bioRxiv - Immunology 2024Quote: ... targeting mouse Pvrl2 or Pvr were cloned into pSpCas9(BB)-2A-GFP plasmid (PX458, ADDGENE). 6 μg of each vector was transfected into tumor cells plated on a 6-well plate using FuGENE6 transfection reagent (Promega ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR products were cloned into the doxycycline-inducible plasmid pCW57-MCS1-2A-MCS2 (Addgene #71782), which was modified by adding bGHpolyA between the MluI and BamHI restriction sites ...
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bioRxiv - Cancer Biology 2024Quote: ... Guide RNAs (gRNAs) were cloned into the pSpCas9n(BB)-2A-GFP (PX461) plasmid (Addgene #48140) and delivered to parental K562 cells by Amaxa nucleofection using the Amaxa Cell line nucleofector kit V (Lonza VCA-1003) ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were transfected with the pCAG-eSpCas9-2A-GFP (generous gift from Jizhong Zou, Addgene plasmid #79145 ...
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bioRxiv - Biochemistry 2024Quote: ... Lambda Phosphatase plasmid was ordered from Addgene (a gift from John Chodera & Nicholas Levinson & Markus Seeliger ...
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bioRxiv - Cell Biology 2023Quote: ... Rat Myo1b (Plasmid #135064, Addgene) C-terminal Myc-tag ...
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bioRxiv - Molecular Biology 2020Quote: ... phosphorylated and cloned into the BbsI sites of the pSpCas9(BB)-2A-GFP (Addgene plasmid # 48138) (Ran et al ...
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bioRxiv - Molecular Biology 2021Quote: ... and also cloned spCas9nuclease with Chd4 sgRNA based on PX458-pSpCas9(BB)-2A-GFP (#48138, Addgene). Then ...
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bioRxiv - Cancer Biology 2019Quote: ... The CRISPR/Cas9 plasmid pSpCas9n(BB)-2A-Puro was a gift from Feng Zhang (Addgene #48141). Lentiviral vectors for GFP-FAK wildtype and FAK kinase-dead (R454 ...
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bioRxiv - Cancer Biology 2019Quote: ... annealed oligos (Supplemental Table 1) were inserted into pSpCas9(BB)–2A–Puro (PX459) V2.0 (Addgene, #62988) using the BbsI (New England Biolabs ...
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bioRxiv - Neuroscience 2020Quote: ... an inverted bicistronic 2A sequence was inserted into pAAV-Ef1α-DIO-EGFP-WPRE-pA (Addgene#37084) upstream of EGFP by PCR linearization and overhang production on pAAV vector ...
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bioRxiv - Neuroscience 2020Quote: ... These two gRNAs were then subcloned into pSpCas9n(BB)-2A-GFP plasmid (pX461; Addgene No: 48140). We made use of Cas9 nickase (Cas9n ...