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Citations for Addgene :
301 - 350 of 1157 citations for Rat Phosphatidic Acid Phosphatase Type 2A PPAP2A ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... These complementary oligos were annealed and cloned into pSpCas9(BB)-2A-Puro vector (Addgene). Plasmids containing guideRNA target sites were confirmed by sequencing ...
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bioRxiv - Molecular Biology 2020Quote: sgRNAs were cloned into the pSpCas9(BB)-2A-Puro(PX459)-V2.0 vector (Addgene #62988) by annealing oligonucleotides 1 and 2 (Table S3 ...
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bioRxiv - Cell Biology 2022Quote: ... Annealed gRNA oligonucleotides were inserted into the pSpCas9n(BB)-2A-GFP plasmid (Addgene, PX458) and the construct was transfected into RPE1 cells using Lipofectamine LTX with PlusReagent (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... Cas9 plasmid (pSpCas9(BB)-2A-BFP (a modified version of PX458 (Addgene plasmid # 48138), in which eGFP is replaced for tagBFP) ...
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bioRxiv - Developmental Biology 2020Quote: ... sgRNAs were cloned into pSpCas9(BB)-2A-Puro (PX459) V2.0 (Feng Zhang Lab; Addgene plasmid #62988 ...
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bioRxiv - Cell Biology 2021Quote: ... The sgRNA complementary oligonucleotide templates were cloned into pSpCas9(BB)-2A-Puro (Addgene, #48139) or pSpCas9(BB)-2A-GFP (Addgene ...
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bioRxiv - Cell Biology 2021Quote: Guide RNA sequences were cloned into pSpCas9(BB)-2A-Puro (PX459) (Addgene plasmid 48139) for expression ...
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bioRxiv - Cell Biology 2021Quote: ... pSpCas9(BB)-2A-GFP (PX458) was a gift from Feng Zhang (Addgene plasmid # 48138). The Hec1-GFP Wt and 9A mutant plasmids were a gift of Jennifer DeLuca ...
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bioRxiv - Cell Biology 2022Quote: ... sgRNAs were subsequently cloned into the pSpCas9(BB)-2A-Puro(PX459)V2.0 vector (Addgene). 2µg/ml puromycin (InvivoGen ...
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bioRxiv - Immunology 2021Quote: ... The sgRNA sequence was cloned into the pSpCas9 (BB)-2A-GFP plasmid (pX458, Addgene). The construct was then independently transfected into HEK293T cells ...
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bioRxiv - Genomics 2019Quote: We used pSpCas9(BB)-2A-Puro (PX459) V2.0 [a gift from Feng Zhang (Addgene plasmid # 62988 ...
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bioRxiv - Genetics 2019Quote: pSpCas9(BB)-2A-Puro (PX459) was used to construct CRISPR/Cas9 vectors (Addgene 48139). The following gRNA oligos were cloned into the BbsI restriction site:
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bioRxiv - Cancer Biology 2020Quote: The iBRD4 plasmids were constructed using the pCW57-GFP-2A-MCS backbone (Addgene, 71783), which was a gift from Adam Karpf and previously described(Barger et al. ...
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bioRxiv - Microbiology 2020Quote: ... pSpCas9(BB)-2A-GFP (pX458) was a gift from Feng Zhang (Addgene plasmid # 48138) (Ran et al ...
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bioRxiv - Molecular Biology 2019Quote: ... The sequences were cloned into the pX458 vector (pSpCas9BB-2A-GFP; obtained from Addgene). Cells were co-transfected with this vector and a repair template spanning the K164 genomic locus but containing the K164R mutation (AAA-AGA codon change) ...
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bioRxiv - Genetics 2021Quote: ... pSpCas9(BB)-2A-GFP (PX458) was a gift from Feng Zhang (Addgene plasmid # 48138). The homology-directed repair template was designed based on the pFETCh_Donor plasmid from the CETCh-seq protocol (Savic et al. ...
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bioRxiv - Genetics 2020Quote: ... and the Cas9-BGH fragment from pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene; 62988) (Ran et al ...
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bioRxiv - Genetics 2020Quote: ... gRNA-containing plasmids were generated in pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene; 62988) (Ran et al ...
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bioRxiv - Cell Biology 2021Quote: ... and selected guide sequences cloned into pSpCas9(BB)-2A-Puro (Addgene plasmid ID: 48139). Following overnight transfections (described above) ...
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bioRxiv - Neuroscience 2021Quote: ... gRNAs were cloned into the vector pSpCas9(BB)-2A-GFP (PX458) (Addgene cat#48138). The CRISPR MDGA2 resitant sequence was the same as for shMDGA2 since the gRNA for MDGA2 was directed to the signal peptide ...
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bioRxiv - Biochemistry 2021Quote: ... was cloned between Bbsl sites of the pSpCas9(BB)-2A-Puro-V2.0 (62988, Addgene). The plasmid pUC57-NASP-FKBP12F36V ...
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bioRxiv - Developmental Biology 2020Quote: ... gRNAs were individually cloned into pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene plasmid #62988) (Ran et al. ...
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bioRxiv - Cell Biology 2021Quote: ... pSpCas9(BB)-2A-GFP (PX458) was a gift from Feng Zhang (Addgene plasmid #48138) (Ran et al. ...
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bioRxiv - Cell Biology 2019Quote: ... Annealed double strand DNAs were ligated into pSpCas9(BB)-2A-GFP (PX458) vector (Addgene) at the Bpi1 (Bbs1 ...
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bioRxiv - Immunology 2020Quote: ... phosphorylated and ligated into the vector pSpCas9(BB)-2A-Puro (PX459 Addgene plasmid #62988) V2.0 (a gift from Feng Zhang ...
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bioRxiv - Genomics 2021Quote: ... the guide RNA (sgRNA) was cloned into the pSpCas9(BB)-2A-GFP (PX458; Addgene) vector36 ...
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bioRxiv - Genomics 2021Quote: ... and cloned into the pSpCas9(BB)-2A-Puro (PX459) V2.0 plasmid (Addgene plasmid #62988) as described in Ran et al ...
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bioRxiv - Biochemistry 2022Quote: ... combining two complementary oligonucleotides into D10ACas9 vector pX461-PSPCas9N(BB)-2A-GFP (Addgene #48140). For the inducible expression of H3-APEX-GFP ...
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bioRxiv - Cell Biology 2022Quote: ... pSpCas9(BB)-2A-GFP (PX458) was a gift from Feng Zhang (Addgene plasmid #48138). HeLa WT cells were transfected with the guide containing plasmid using the transfection reagent XtremeGENE HP (Roche ...
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bioRxiv - Immunology 2022Quote: ... pSpCas9(BB)-2A-GFP (PX458) was a gift from Feng Zhang (Addgene plasmid #48138) following the Zhang lab ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μL of AAV9-hsyn-cre-2a-tdT (Addgene#107738, titer ∼1×10^13) was diluted with 3 μL of PBS and was injected into the subarachnoid space of one hemisphere at the level of somatosensory cortex in Mtor-floxed-5XFAD mice ...
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bioRxiv - Neuroscience 2022Quote: ... and ligated into the BbsI site of pSpCas9(BB)-2A-GFP plasmid (Addgene, #48138) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The gRNA was inserted into the pSpCas9(BB)-2A-GFP vector (PX458; Addgene #48138) following a previously published protocol (Ran et al. ...
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bioRxiv - Bioengineering 2023Quote: ... were cloned into a backbone derived from pXR001: EF1a-CasRx-2A-EGFP (Addgene #109049) to replace the CasRx coding sequence ...
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bioRxiv - Cell Biology 2023Quote: This guide sequence was cloned into pSPCas9(BB)-2A-puro(PX459)V2.0 (Addgene, #62988) as described previously [83] to create pSPCas9(BB)-2A-puro(PX459)-hMyo10 ...
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bioRxiv - Cell Biology 2023Quote: ... The pAAV-EFS-Cas13d-2A-EGFP plasmid was derived from pXR001 (Addgene ID: 109049). The CAG-mCherry-NKCC1 plasmid was based on pcDNA3.1(+)-C-eGFP ...
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bioRxiv - Genetics 2023Quote: ... followed by cloning in the BbsI-digested pSpCas9(BB)-2A-GFP (PX458) (Addgene, #48138) and the pU6 (BbsI)_CBh-Cas9-T2A-mCherry (Addgene ...
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bioRxiv - Neuroscience 2023Quote: ... These sgRNA targets were cloned into pSpCas9(BB)-2A-Puro (AddGene plasmid ID 48139) as described.30 mES cells were trypsinized with 0.05% trypsin ...
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bioRxiv - Cell Biology 2023Quote: ... Annealed oligonucleotides were inserted into pSpCas9(BB)-2A-Puro (PX459) V2.0 vector (#62988; Addgene) digested with BbsI ...
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bioRxiv - Cancer Biology 2023Quote: ... Each guide was cloned into a pSpCaV9(BB)-2A-GFP vector (Addgene, cat# 48138) and transfected into cells of the non-rejected SCCs ...
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bioRxiv - Genomics 2023Quote: ... sgRNA annealed oligos were ligated into pSpCas9(BB)-2A-Puro (PX459 v2.0) (Addgene; 62988) following BbsI (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: pSpCas9(BB)-2A-GFP (PX458) was a gift from Feng Zhang (Addgene plasmid #48138) 12 ...
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bioRxiv - Molecular Biology 2023Quote: dCas9: Lv EF1a dCas9-HA 2A Blast was a gift from Jerry Crabtree (Addgene plasmid # 102812 ;
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bioRxiv - Cell Biology 2023Quote: ... PhOTO-M (pMTB:memb-Dendra2-2A-H2B-Cerulean) was a gift from Periklis Pantazis (Addgene plasmid # 92401 ...
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bioRxiv - Developmental Biology 2023Quote: ... sgRNAs were constructed to pSpCas9n(BB)-2A-Puro vector (PX462, Addgene plasmid no. 62987). 4 μg of the plasmid was transfected using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Genetics 2024Quote: ... 1 μg of the lentiviral transfer vector G1088E_pLenti-CMV-mNeonGreen-2A-HygroR (Addgene #216279), and 1 μg of one of six spike proteins tested.
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bioRxiv - Genetics 2024Quote: ... Plasmid pSpCas9(BB)-2A-Puro (PX459) V2.0 was a gift from Feng Zhang (Addgene plasmid # 62988 ...
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bioRxiv - Cell Biology 2024Quote: ... The sequences were cloned into pSpCas9(BB)-2A-Puro (PX459) vector (Addgene plasmid #62988), followed by lipofectamine-mediated transfection into the MCF10A cells ...
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bioRxiv - Molecular Biology 2023Quote: ... Gene-specific gRNAs were integrated into pSpCas9(BB)-2A-GFP (PX458) (Addgene no. 48138) and 2μg of plasmid was electroporated into 106 cells using a Lonza 4D-Nucleofector according to the manufacturer’s protocol for HCT116 cells ...
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bioRxiv - Molecular Biology 2024Quote: ... 5μg lentiviral guide expression vector pKLV2-U6gRNA5(BbsI)PGKpuro-2A-BFP-W (Addgene #67974) was linearised with 100 U of Bbs I-HF (NEB ...