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Citations for Addgene :
351 - 400 of 2124 citations for Rat Carboxypeptidase A3 Mast Cell CPA3 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... One million cells were transfected with the corresponding pools of guide RNAs and with the pX458 plasmid encoding for Cas9 and GFP proteins (48138, Addgene), using lipofectamine 2000 ...
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bioRxiv - Biochemistry 2023Quote: ... the C162A mutant and the C162L mutant were independently cloned and expressed as 6× His-SUMO fusion proteins from the expression vector pAL (Addgene). BL21 (DE3 ...
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bioRxiv - Biochemistry 2023Quote: OsKAI2 and the OsKAI2int mutant were independently cloned and expressed as GST (Glutathione-S-Transferase) fusion protein from the expression vector pCOOL (Addgene). BL21 (DE3 ...
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bioRxiv - Microbiology 2024Quote: ... using an engineered plasmid that encodes a deGFP protein and a malachite green RNA aptamer (PT7-deGFP-MGapt was a gift from Richard Murray, via Addgene plasmid # 67741 ...
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bioRxiv - Cell Biology 2019Quote: Stable Cas9-expressing cells were generated by transducing BeWo cells with lentiCAS9-blast (Addgene, cat. 52962). To generate TMEM16F/ANO6 knockout cells ...
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bioRxiv - Systems Biology 2020Quote: ... MDCKI + bPAC cells were generated by infecting MDCKI cells with pLenti-PGK-bPAC::NES (Addgene #130267). PKAc expressing cells were obtained by transfecting MDCKI and MDCKI + bPAC cells with pPB-CAG-PRKACA::mRuby2 (Addgene #130268) ...
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bioRxiv - Cell Biology 2019Quote: For generation of lentiviral knockout and overexpression cells HEK293T cells were transfected with psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259 ...
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bioRxiv - Immunology 2022Quote: ... KMS11-Cas9+ cells and LP1-Cas9+ cells (transduced with pLX 311-Cas9 construct, Addgene plasmid # 96924) were obtained from the Broad Institute ...
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bioRxiv - Genomics 2022Quote: ... and NIH/3T3 cells (Addgene #138149), as well as doxycycline-inducible nuclease-inactive RfxdCas13d-NLS HEK293FT ...
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bioRxiv - Microbiology 2020Quote: ... and AdEasier-1 cells (Addgene #16399) were a gift from Bert Vogelstein (He et al ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells harboring plasmid pKM208 (Addgene #13077) were cultured at 30°C to an O.D ...
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bioRxiv - Microbiology 2023Quote: ... Electrocompetent cells containing pREDCas9 (Addgene #71541) were generated as previously described[68] ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli MG1655 K12 cells (Addgene #37854) transformed with reporter ...
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bioRxiv - Immunology 2023Quote: ... Phoenix-Eco cells (Addgene CRL-3214) were used for retrovirus production ...
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: ... the destination plasmids were transfected with the three required viral protein plasmids: pMDLg/pRRE (gift from Didier Trono; Addgene plasmid # 12251), pVSVG (gift from Bob Weinberg ...
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bioRxiv - Molecular Biology 2021Quote: ... The mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8xHis tag was obtained from Addgene (#145145). The mammalian expression vector for soluble ACE2 pcDNA3-sACE2 (WT)-sfGFP (#145171 ...
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bioRxiv - Molecular Biology 2021Quote: We performed a genome-wide CRISPR knock-out (KO) screen using the lentiviral Brie sgRNA library comprising 4 sgRNAs per protein-coding gene (Addgene #73632) (Doench et al. ...
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bioRxiv - Microbiology 2020Quote: ... Vesicular stomatitis virus G protein (VSV-G) pseudotyped lentiviruses expressing human ACE2 were produced by transient co-transfection of pMD2G (Addgene #12259) and psPAX2 (Addgene #12260 ...
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bioRxiv - Neuroscience 2020Quote: Cortical pyramidal neurons (CPN)s in WT and YAC128 cultures were labeled by transfecting a subset of neurons (1 of 2.7 million) with a cytoplasmic green fluorescent protein (GFP) (Addgene plasmid 37825) at the time of platting ...
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bioRxiv - Developmental Biology 2022Quote: ... Plasmids containing coding regions for the fast-folding fluorescent proteins sCFP3A and Venus/YFP (Balleza et al., 2018) were obtained from Addgene (www.addgene.org). Partial or complete coding regions for Can-elt-3 ...
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bioRxiv - Microbiology 2022Quote: ... with a 15 base pair overlap with vector backbone were used in a polymerase chain reaction (PCR) to generate fast Timer protein timer fragment from plasmid pFast-FT-N1 (Addgene 31910). Five tandem repeats of Tax responsive element (TRE ...
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bioRxiv - Biochemistry 2022Quote: ... DNA encoding peptides derived from the C-terminal last 10 residues of the norovirus and cellular proteins were cloned into the pET-His-GST vector (Addgene:29655) with an N-terminal GST tag ...
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bioRxiv - Molecular Biology 2022Quote: U-2 OS CRISPR knockout lines were generated using the All-in-One plasmid encoding dual sgRNAs and fluorescent protein-coupled Cas9D10A nickase (AIO-GFP; Addgene #74119) (Chiang et al. ...
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bioRxiv - Cell Biology 2022Quote: ... The Capping Protein (F-actin-capping protein subunit alpha-1 and F-actin-capping protein subunit beta) plasmid was a gift from Antoine Jégou & Guillaume Romet-Lemonne (Addgene plasmid #89950).
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bioRxiv - Cell Biology 2022Quote: ... The mammalian expression plasmid for Ism1 with C-terminal myc-6xhis tag plasmid for recombinant Ism1 protein production was from Addgene (#173046).
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bioRxiv - Cell Biology 2022Quote: ... was made by replacing the luciferase sequences with the fluorescent protein T-Sapphire in pHAGE NFKB-TA-LUC-UBC-dTomato-W (Addgene #49335). Additionally we replaced the marker tdTomato in Addgene #49335 with the resistance cassette for HygromycinB ...
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bioRxiv - Plant Biology 2021Quote: ... and Cas9 RNP nucleofection were performed according to Huang et al.30 Cas9 recombinant protein was overexpressed in Escherichia coli BL21 harboring the plasmid pMJ915 (Addgene # 69090). Cas9 protein was purified and stored at −80°C in Cas9 RNP buffer (20 mM HEPES at pH 7.5 ...
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bioRxiv - Immunology 2019Quote: ... The PresentER plasmid encoding a signal peptide from Mouse Mammary Tumor Virus envelope protein followed by the SIINFEKL epitope followed by mCherry was obtained from Addgene (#102945),a kind gift of D ...
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bioRxiv - Immunology 2020Quote: We using the 3*Flag sequence to replace the GFP protein in the pLenti CMV GFP Puro vector (Addgene, 658-5) for adding some Restriction Enzyme cutting site (XbaI-EcoRV-BstBI-BamHI ...
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bioRxiv - Cell Biology 2020Quote: ... was made by amplifying TPD54 by PCR from human Tumor protein D54 (IMAGE clone: 3446037) and inserting into pIRES-EGFP-puro (Addgene #45567) via NheI and XhoI ...
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bioRxiv - Cell Biology 2020Quote: ... HeLa cells were transfected with a plasmid that expresses RNAi-resistant ch-TOG:GFP plus a hairpin RNA (sh ch-TOG) to knockdown endogenous protein and minimize overexpression (Addgene ID# 69113). HeLa cells stably expressing GFP:tubulin were previously generated (van Oostende Triplet et al. ...
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bioRxiv - Cell Biology 2021Quote: ... the cDNA sequence encoding the third generation rtTA protein (rtTA3) was obtained by PCR from plasmid MXS_PGK::rtTA3-bGHpA (Addgene plasmid #62446) (Sladitschek and Neveu ...
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bioRxiv - Microbiology 2022Quote: GFP and myc-tagged 14-3-3ε were generated as follows: a G-block containing the full-length 14-3-3ε protein (IDT) was cloned into pEGFP-C1 (Addgene #2487) using XhoI and BamHI restriction sites ...
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bioRxiv - Cell Biology 2022Quote: ... reporter under the constitutive elongation factor 1-α (EF1α) promoter and upstream of a P2A linker followed by the tdTomato fluorescent protein (gift from Kazuhiro Oka;Addgene plasmid #72486 ...
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bioRxiv - Systems Biology 2022Quote: ... Transfection with siRNA or the RFP-tagged protein of interest was performed 12 hours prior to transfection with I-SceI (Addgene #26477) or GFP control (Addgene #89684) ...
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bioRxiv - Molecular Biology 2023Quote: ... An AAV that only expressed the mCherry protein under the same neuronal promoter was use as control condition (Addgene Plasmid #114472). Wildtype and Cdr1as-KO neurons were infected at DIV4-6 by directly adding the viral particles into the culturing media at a titer of 109 VG/ml ...
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bioRxiv - Physiology 2022Quote: ... reporter under the constitutive elongation factor 1-α (EF1α) promoter and upstream of a P2A linker followed by the tdTomato fluorescent protein (gift from Kazuhiro Oka;Addgene plasmid # 72486 ...
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bioRxiv - Microbiology 2022Quote: To make human ACE2 protein, pcDNA3-sACE2-WT(732)-IgG1 (Chan et al., 2020) (Addgene plasmid #154104, gift of Erik Procko) plasmid was transfected into Expi293 cells using PEI at a ratio of 1:3 ...
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bioRxiv - Molecular Biology 2023Quote: ... and its cognate transactivator protein (3G) gene (derived from AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep, a gift from Yannick Doyon: Addgene plasmid #92099; Dalvai et al., 2015). The resulting plasmid was named as DOGGp in this work ...
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bioRxiv - Cell Biology 2023Quote: A synthetic sequence corresponding to 2A peptide from Thoseaasigna virus capsid protein and BspTI (AflII) restriction site was first cloned to pCXLE-EGFP (Plasmid #27082, Addgene, www.addgene.org) (Okita et al. ...
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bioRxiv - Biochemistry 2023Quote: ... Human ATAD2B bromodomain-containing protein (residues 953−1085, Uniprot code: Q9ULI0) was a gift from Nicole Burgess-Brown (Addgene plasmid # 39046) was PCR-amplified and cloned into pDEST15 (GlaxoSmithKline ...
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bioRxiv - Genomics 2023Quote: ... CROP-seq-opti-Puro-T2A-GFP was assembled by adding a T2A-GFP downstream of Puromycin resistant protein coding sequence on the CROP-seq-opti plasmid (Addgene #106280). Flanking MluI and CsiI digestion sites were added to the GFP Gblock (IDT ...
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bioRxiv - Immunology 2023Quote: ... The medium of the HEK 293T cells was exchanged with 1 mL fresh complete DMEM and cells were transiently co-transfected with four plasmids: 0.25 µg of a plasmid encoding the retroviral structure Gag-Pol proteins (pMDLg/pRRE, Addgene plasmid #12251), 0.5 µg of a plasmid encoding the vesicular stomatitis virus G protein (pMD2.G ...
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bioRxiv - Genomics 2023Quote: ... and HA-tag were introduced by CRISPR/Cas9-mediated homologous recombination to the N-terminus of ZBTB24 protein (gRNAs are listed in Table S9 were cloned in pSpCas9(BB)-2A-GFP (PX458) (Addgene #48138), the plasmids containing HA tag ...
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bioRxiv - Genomics 2023Quote: ... and flanking regions of respective ER localized proteins were inserted into a backbone containing a UCOE-EF-1α promoter and a 3′ WPRE element (Addgene #135448) (Jost et al. ...
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bioRxiv - Biochemistry 2022Quote: ... Immobilized proteins were eluted by incubation with 1 mL of 250 nM SENPEuB protease (expressed and purified from pAV286 (Addgene # 149333))74 overnight at 4° C ...
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bioRxiv - Cancer Biology 2023Quote: ... we replaced the VP64-P65-Rta sequence (encoding transcription activator complex) in the lentiviral vector of dCas9-VPR-mCherry fusion protein (for CRISPRa, Addgene #102245) with the KRAB-MECP2 sequence from the transient expression vector of dCas9-KRAB-MECP2 (for CRISPRi ...
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bioRxiv - Developmental Biology 2023Quote: ... the destination plasmids were transfected with the three required viral protein plasmids: pMDLg/pRRE (gift from Didier Trono; Addgene plasmid # 12251), pVSVG (gift from Bob Weinberg ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the mouse synaptophysin-mRuby fusion protein was cloned into the generated synapsin:eGFP transgene from AAV-FLExloxP-mGFP-2A-synaptophysin-mRuby (Addgene Plasmid# 71760) plasmid vector courtesy of Liqun Luo (Beier et al. ...
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bioRxiv - Neuroscience 2023Quote: ... The construct encoding SARS-CoV-2 matrix (M) protein was cloned by PCR amplification of M cDNA from the pDONR 207 SARS-CoV-2 M plasmid (Addgene #141274) using a Forward primer inserting a restriction site for EcoRI and a reverse primer bearing a restriction site for BamHI and a Myc tag ...