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Citations for Addgene :
551 - 600 of 2285 citations for Rat Carboxypeptidase A3 Mast Cell CPA3 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... reporter cell lines were transfected with pCBASce (Addgene plasmid #26477 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with mCherry-RAB5 Q79L (Addgene #35138 ...
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bioRxiv - Cancer Biology 2020Quote: Generation of batch MTR knockout cells in each cell line was achieved using the lentiviral CRISPR–Cas9 vector system LentiCRISPR v2 (Addgene #52961). Briefly ...
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bioRxiv - Biochemistry 2020Quote: HEK293T CRISPR-Cas9 cells were generated by transfecting wild-type 293T cells with pX330-U6-Chimeric_BB-CBh-hSpCas9 vector (Addgene, Plasmid# 42230) carrying single-guide RNA (sgRNA ...
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bioRxiv - Microbiology 2020Quote: RAW 264.7 cells stably expressing FL-Cas9 were generated by transducing RAW 264.7 cells with lentivirus containing LentiCas9-Blast (Addgene plasmid #52962)(Sanjana et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... 22Rv1 STEAP1 ko and PC3 STEAP1 ko cells were generated by transient transfection of 22Rv1 cells with a pool of PX458 (Addgene, #48138) plasmids each expressing one of four different sgRNA targeting sequences predicted from the Broad Institute Genetic Perturbation Platform sgRNA Designer63 ...
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bioRxiv - Genomics 2020Quote: In order to create stable dCas9-KRAB expressing cell line LNCaP cells were infected with lenti-KRAB-dCas9-blast (Addgene, 89567) and selected with 6 µg/ml blasticidin for two weeks ...
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bioRxiv - Cell Biology 2021Quote: ... WT and Lig4-/- abl pre-B cells and MCF10A human mammary epithelial cells used in this study all contain pCW-Cas9 (Addgene# 50661), which has a FLAG-tagged Cas9 cDNA under the control of a doxycycline-inducible promoter ...
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bioRxiv - Microbiology 2021Quote: ... Derivatives of HEK-293T cells expressing ACE2 were generated by transducing HEK-293T (ATCC, CRL-3216) cells with ACE2 (Addgene, #145839). Cells were used as single cell clone was derived by limiting dilution from the bulk populations ...
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bioRxiv - Cancer Biology 2022Quote: ... These constructs were introduced into cells generated by lentiviral transduction of MCF10A TP53-/- cells with lenti-BLAST-dCas9-KRAB (Addgene, #89567) followed by selection with 10 μg/mL blasticidin.
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bioRxiv - Cell Biology 2022Quote: An OgtfloxedCre-ERT2+/KI Rosa26-LSL-YFP+/KI mESC cell line stably expressing Cas9 was generated by lentiviral transduction of the parental cells with lentiCas9-Blast (Addgene #52962). CRISPR sgRNA library lentiGuide-Puro-Brie (Addgene ...
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bioRxiv - Microbiology 2024Quote: HeLa-TetR-Cas9 clonal cells used in the genome-wide screen and polyclonal Huh7 cells transduced with 311-Cas9 (Plasmid Addgene #96924) and selected using 10 µg/mL blasticidin for 7 days were used for secondary screening ...
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bioRxiv - Genetics 2023Quote: We plated 550,000 HEK293T cells on 6-well plates and 24 hours later we transfected the cells with 900 ng psPAX2 packaging vector (Addgene #12260), 360 ng pMD2.g VSV-G envelope vector (Addgene #12259) ...
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bioRxiv - Cell Biology 2023Quote: ... LS174T ERN1-/- cells and LS174T ERN2-/- cells were constructed by transfection with pSpCas9(BB)-2A-GFP (Feng Zhang lab, Addgene #48138) containing sequences for guides targeting exon 8 (WGE ID’s 1150303698 and 1150303744 for ERN1 ...
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bioRxiv - Genetics 2023Quote: ... sgRNAs were cloned into a novel construct that had previously been developed for the pooled single-cell RNA sequence profiling of primary cells (CROPseq-Guide- pEFS-SpCas9-p2a-puro; Addgene: #99248).20 The lentivirus was then packaged by transfecting HEK 293FT cells with pCMV delta 8.91 ...
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bioRxiv - Cell Biology 2019Quote: Fluorescent protein sequences were obtained from lab stocks with the exception of mTagBFP2 which was amplified from mTagBFP2-pBAD (Addgene, Watertown, USA; plasmid ID 54572). The 3mTagBFP2 was a kind gift from Serge Pelet (University of Lausanne ...
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bioRxiv - Cell Biology 2020Quote: ... Stable expression of fluorescently tagged ciliary and centriolar proteins was achieved by modification of pCW-Cas9 using cDNAs encoding ARL13B (Addgene #40879, gift from Tamara Caspary), EHD1 (gift from Chris Westlake) ...
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bioRxiv - Cancer Biology 2021Quote: ... Flp-In 3T3 p53-/- cell line was generated by Crispr/Cas9 by co-transfecting Flp-In 3T3 cells with px330 p53 plasmid (Addgene Plasmid #59910) expressing Cas9 and sgRNA targeting mouse p53 and pmaxGFP plasmid (Lonza) ...
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bioRxiv - Cancer Biology 2020Quote: ... To generate inducible CRISPR-Cas9 GFPT2 KO cell lines, parental cells (H460, H157, Calu-1) were first infected by pCW-Cas9 plasmid (Addgene plasmid #50661), sorted by puromycin selection ...
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bioRxiv - Cell Biology 2021Quote: Stock of lentiviral particles were obtained by transfection of HEK293T cells (2×106 cells) with 2 μg of lentivector plasmid lentiCRISPRv2 (a gift from Feng Zhang, Addgene plasmid, 52961) expressing single guide RNA targeting an exon within ATG5 gene ...
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bioRxiv - Cancer Biology 2021Quote: ... For generation of KO cell line pools PC-9 and HCC827 cells were transduced with pKLV2-EF1a-Cas9Bsd-W (Addgene ID:68343)[71] to stably express Cas9 ...
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bioRxiv - Biochemistry 2022Quote: ... For transient HER2 overexpression in HEK293 cells, the cells were transfected with a plasmid encoding human HER2 wildtype (Li et al., 2004)(Addgene plasmid #16257) the day before measurement with removal of the transfection reagent after 6 hours ...
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bioRxiv - Cell Biology 2022Quote: ... The S1R-Apex and GFP-Apex plasmids were co-transfected into either HeLa cells (ATCC; CCL-2) or HEK293T cells (ATCC; CRL-3216) along with pXAT2 (Addgene plasmid 80494) (30) ...
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bioRxiv - Molecular Biology 2020Quote: DR-GFP-HCT116 and DR-GFP-U2OS reporter cells were generated by electroporating HCT116 and U2OS cells with Sac1/Kpn1 linearized pHPRT-DRGFP (43) (Addgene, plasmid #26476) DNA ...
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bioRxiv - Cell Biology 2022Quote: ... we first generated a doxycycline-inducible Cas9-expressing THP-1 cell line (iCas9-expressing cells) by transducing THP-1 cells with lentiviruses carrying the Lenti-iCas9-neo plasmid (a gift from Qin Yan; Addgene plasmid #85400). Multiple guide RNAs targeting a gene were cloned into the Lenti-multi-Guide plasmid (a gift from Qin Yan ...
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bioRxiv - Immunology 2019Quote: ... expressing 4T1 tumor cells were generated by transducing cells with lentiviral vector carrying GFP gene (phage ubc nls ha pcp gfp plasmid, Addgene Plasmid #64539).
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bioRxiv - Cell Biology 2021Quote: ... CRISPR-edited NRF2-KO HaCaT cells were produced by transfecting HaCaT cells with pLentiCRISPR-v2 (a gift from Dr Feng Zhang, Addgene plasmid #52961) containing a guide RNA directed against the exon 2 of the NFE2L2 locus (which encodes NRF2 ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 × 106 cells of each cell line were electroporated with a mixture of 10 μg of plasmid pEGFP-D50 lamin A (Addgene plasmid #17653) that had been linearized with EagI and 1 μg of pBABE-puro (Addgene plasmid #1764 ...
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bioRxiv - Molecular Biology 2023Quote: ... Human TMPRSS2 expression in VeroE2T and in VeroE6 cells (VeroE6-TMPRSS2) was achieved by infecting the cells with a 2nd generation lentiviral vector pLEX307-TMPRSS2-blast (Addgene plasmid #158458) as a transfer vector ...
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bioRxiv - Cell Biology 2023Quote: ... Primary MEFs used for the long term cell migration and JLY experiments were infected with viral media from HEK cells transfected with 3x-mScarlet-FTractin (Addgene Plasmid #112960)(53) ...
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bioRxiv - Cell Biology 2023Quote: ... MCF-7 or HeLa single cell colonies were selected from cells transduced with the following sequences targeting human genes cloned into lentiCRISPR v2 (Addgene no. 52961):
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bioRxiv - Cancer Biology 2021Quote: ... CRISPR generated cells were transduced with lentiCRISPRv2 (Addgene # 98290) cloned with specific guides targeting tRNAIleUAU loci (Guide 1 ...
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bioRxiv - Cell Biology 2021Quote: ... Then cells were transfected with PsPAx2 (Addgene Plasmid #12260), and pMD2.G (Addgene Plasmid #12259) ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were first infected with dCas9 (Addgene, 61425-LV) and colonies were selected with blasticidin ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were transfected the next day with pRenilla (Addgene) and empty pGL3 vector or pGL3 vector containg the DUSP6 promoter regions 191p and 508p using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... 293T packaging cells were transfected with psPAX2 (Addgene 12260), pMD2.G (Addgene 12259 ...
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bioRxiv - Cell Biology 2020Quote: HEK293 cells were transfected with pMIB1-FLAG (Addgene# 37116) and pHA-Ub (Addgene# 18712 ...
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bioRxiv - Cell Biology 2020Quote: ... KO cells were generated using either px330 (Addgene #42230) or pLentiCRISPRv2 (Addgene #52961) ...
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bioRxiv - Bioengineering 2019Quote: Images of HEK cells co-expressing hGlut1 (Addgene #18085) and iGlucoSnFR.mRuby2 were acquired on an inverted Zeiss LSM 800 confocal microscope equipped with GaAsP detectors and an EC Plan-Neofluar 10x/0.30 M27 objective ...
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bioRxiv - Cell Biology 2021Quote: ... U2OS cells were transfected with GFP-Sec61β (Addgene, 15108) using FuGENE6 (Promega) ...
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bioRxiv - Genetics 2020Quote: ... and cells were nucleofected with Cas9-GFP (Addgene #44719), a gBlock (IDT ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli S2060 cells were prepared containing pJC175e (Addgene #79219), a plasmid expressing pIII under control of the phage shock promoter32 ...
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bioRxiv - Cancer Biology 2020Quote: We infected cells with pCW-Cas9 plasmid (Addgene #50661) and selected cells with puromycin at 2 μg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transfected with GFP-tagged galectin 3 (Addgene) or a mutant variant of it ...
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bioRxiv - Cell Biology 2020Quote: ... we transfected HEK293T cells with FUW-OSKM (Addgene #20328) and packaging vectors using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells transduced with lentivirus carrying KRAB-dCas9 (Addgene #71236) and sgRNAs were harvested one week after the transduction ...
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bioRxiv - Cancer Biology 2022Quote: ... cells with pMD2.G and psPAX2 packaging plasmids (Addgene) to create VSV-G pseudotyped lentiviral particles ...
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bioRxiv - Cancer Biology 2022Quote: HEK293T cells were transfected with lentiCRISPRv2 (Addgene Plasmid #98290), the above dual sgRNA constructs ...
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bioRxiv - Cancer Biology 2022Quote: ... 4292 cells were infected with lentiCas9-Blast (Addgene #52962). Following blasticidin selection ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were lentivirally transduced with CRISPR_V2-based vectors (Addgene #52961 ...