Labshake search
Citations for Addgene :
451 - 500 of 2124 citations for Rat Carboxypeptidase A3 Mast Cell CPA3 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... Cells were transduced with psPAX2 (Addgene, 12260), pVSVg ...
-
bioRxiv - Cell Biology 2021Quote: Plasmids encoding the SARS-CoV-2 open reading frames proteins and eGFP control were a kind gift of Nevan Krogan (Addgene plasmid #141367-141395). Plasmids were acquired as bacterial LB–agar stabs ...
-
bioRxiv - Neuroscience 2021Quote: ... and SAD-B19 helper proteins (N, 52.11 mg, Addgene 59924; P, 30.15 mg, Addgene 59925; L, 23.70 mg, Addgene 59922; G, 20.26 mg, Addgene 59921). Cells were maintained with DMEM with GlutaMAX supplement ...
-
bioRxiv - Biochemistry 2020Quote: ... encoding the ELOVL7 protein (Uniprot ID: A1L3×0) was cloned into the baculovirus transfer vector pFB-CT10HF-LIC (available from The Addgene Nonprofit Plasmid Repository) for expression in Sf9 cells (Thermo-Fisher Scientific ...
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... vector expressing a constitutively active form of AMPKa (AMPKα21-312) and GFP (Green Fluorescent Protein) or a control AAV vector (Addgene: plasmid # 60127 and # 50465) were injected bilaterally in the BLA at P35 ...
-
bioRxiv - Immunology 2023Quote: Expi293F cells were cultured and transiently transfected with the pLenti-palmGRET reporter plasmid encoding the dual reporter palmitoylated EGFP-nanoluciferase protein (PalmGRET) (Addgene plasmid #158221 11) for EV production as described previously 23 ...
-
bioRxiv - Bioengineering 2024Quote: ... pET28a-SUMO-SpyTag003 (N-terminal His6 tag-SUMO protein-SpyTag003) was cloned previously by Irsyad Khairil Anuar (University of Oxford) (GenBank and Addgene deposition in progress). pDEST14-SpyCatcher003-TEVs-SpyTag003DA (‘Masked SpyCatcher0003’ ...
-
bioRxiv - Molecular Biology 2021Quote: ... PUS7 and TRUB1 knock out (KO) cell lines were generated by transfecting 293T cells with pLentiCRISPRv2 (Addgene Cat #52961), encoding Cas9 and the following single guide RNAs (sgRNAs) ...
-
bioRxiv - Systems Biology 2020Quote: ... PKAc expressing cells were obtained by transfecting MDCKI and MDCKI + bPAC cells with pPB-CAG-PRKACA::mRuby2 (Addgene #130268). Cells were maintained in MEM (Gibco #11095072 ...
-
bioRxiv - Neuroscience 2020Quote: ... Dissociated single cell forebrain NPCs were plated 1,000,000 cells/well on 12 well plates and transfected with lentiGuide-tdTomato (Addgene #99376) plasmid and selected by hygromycine ...
-
bioRxiv - Cell Biology 2020Quote: The PIK3C2A knockout cell line was generated by transfecting HeLa cells with pSpCas9(BB)-2A-GFP (pX458, Addgene #48138) into which a single guide (5’-CACCGAGCACAGGTTTATAACAAGC-3’ ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells stably expressing the Mito-DsRed mitochondrial probe were generated by infecting cells with pLV-mitoDsRed (Addgene, plasmid 44386) lentivirus particles with puromycin (2 μg/mL ...
-
bioRxiv - Cancer Biology 2023Quote: HT29 dCas9-VP64 cells were generated by lentiviral transduction of HT29 cells with Lenti-dCas9-VP64-Blast (Addgene, 61425) in the presence of 8 μg/ml polybrene (Santa Cruz ...
-
bioRxiv - Biochemistry 2024Quote: ... Cells were subcultured twice a week at a ratio of 1:5 to 1:8.Transient transfection of HeLa cells (1–2 x 105 cells/6-well) with pcDNA3.1(+) NAPstar or pSC2 HyPer7 (Addgene; plasmid #136466 ...
-
bioRxiv - Biophysics 2022Quote: Imaging of actin cytoskeleton and microtubule network in life cell experiments was performed using NIH 3T3 stable cell line expressing Lifeact-GFP (Addgene) treated with SiR-tubulin (Spirochrome) ...
-
bioRxiv - Cancer Biology 2020Quote: Single-cell clones of Cas9-expressing CALM-AF10 cells were established by cloning in a Lenti-Cas9-2A-Blast vector (Addgene: #73310 ...
-
bioRxiv - Microbiology 2022Quote: ... MGAT1 single KO cells were used to generate MGAT1/C1GALT1 DKO cells and MGAT1/UGCG DKO cells with the pLentiSpBsmBI sgRNA Hygro vector (#62205, Addgene). MGAT1/C1GALT1 DKO cells were used to generate MGAT1/C1GALT1/UGCG TKO cells with the lentCRISPRv2 neo vector (#98292 ...
-
bioRxiv - Immunology 2020Quote: ... Generation of shRNA IFIT1 cells: 293T cells were co-transfected with psPax2 and pMD2.G (Addgene plasmids #12260 and #12259), as well as pLKO.1 (50 ...
-
bioRxiv - Immunology 2021Quote: The Jurkat-NFAT-GFP cell line was generated by transducing Jurkat cells (ATCC, TIB-152) with the pSIRV-NFAT-eGFP plasmid (Addgene, #118031 ...
-
bioRxiv - Immunology 2022Quote: A375-MR1-Cas9 cells generated using the previously described cell line (10) and Lenti Cas9-Blast plasmid (Addgene, Cat#52962) were transduced at 0.3 MOI by both part A and B of the pooled Human GeCKO v2 CRISPR library (Addgene ...
-
bioRxiv - Cell Biology 2022Quote: ... XPA-KO HaCaT cells stably expressing Flag-tagged XPA were generated by transfecting cells with pcDNA4-Flag-XPA (Addgene #22895) and then selecting single clones with geneticin ...
-
bioRxiv - Microbiology 2022Quote: ... MGAT1/C1GALT1 DKO cells were used to generate MGAT1/C1GALT1/UGCG TKO cells with the lentCRISPRv2 neo vector (#98292, Addgene). Primers for sgRNA target sites used here are listed in Table S1 ...
-
bioRxiv - Neuroscience 2023Quote: ... A stably expressing mCherry cell line was utilized for live imaging endothelial cells that was previously developed using pAAVS1-P-CAG-mCh (Addgene).
-
bioRxiv - Bioengineering 2023Quote: PC3-luc cells were derived from PC3 cells transfected with the firefly luciferase expressing plasmid from Addgene (Ubc.Luc.IRES.Puro, RRID:Addgene 33307) (22) ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: 293/hACE2 stable cells were generated by transduction of 293 cells using lentivirus produced in 293T cells utilizing plasmids: pCMV-dR8.2-dvpr (Addgene #8455); pCAGGS-G-Kan (Kerafast EH1017) ...
-
bioRxiv - Cell Biology 2024Quote: Knockout of ATG14 in single-tagged GABARAPs reporter cells was achieved by transfecting the desired cell line with pSpCas9(BB)-2A-Puro (PX459) V2.0 plasmid (Addgene 62988) expressing ATG14-targeting sgRNA ...
-
bioRxiv - Animal Behavior and Cognition 2020Quote: ... Cas9 protein was produced by the Weizmann Institute of Science Protein Purification Unit using the pET-28b-Cas9-His (Alex Schier Lab Plasmids, Addgene, Cambridge, MA, United States) as a template ...
-
bioRxiv - Biophysics 2022Quote: ... the RNA-guided targeting of genes was achieved through coexpression of the Cas9 protein with gRNAs using reagents from the Church group48 (Addgene: http://www.addgene.org/crispr/church/). Targeting sequences of the gRNAs were as followed ...
-
bioRxiv - Molecular Biology 2021Quote: ... HEK293T cells were transfected with lenti-ZsGreen (Addgene) and 10 µg of psPAX2 (Addgene ...
-
bioRxiv - Immunology 2019Quote: ... A375 cells were transduced with lentiCas9-Blast (Addgene) and selected with 5 μg/mL blasticidin for 10 days ...
-
bioRxiv - Neuroscience 2021Quote: ... cells were transfected with CMV-R_GECO (Addgene #45494) (Wu et al. ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cells stably expressing inducible Snail (Addgene plasmid #18798), constitutively active p38 or MAPK14 gRNA targets were generated by transduction of cells as described previously (Mani et al. ...
-
bioRxiv - Cancer Biology 2020Quote: ... cells were co-transfected with HRE-luc (Addgene) (Emerling ...
-
bioRxiv - Biophysics 2020Quote: ... we transfected LentiX cells with psPAX2 (Addgene #12260), pMD2.G (Addgene #12259) ...
-
bioRxiv - Immunology 2022Quote: HEK293A cells stably expressing mouse NLRP3 (Addgene 75127), and ASC-GFP (Addgene 73957 ...
-
bioRxiv - Genetics 2022Quote: ... Jurkat cells containing pLenti-PE2-BSD (Addgene, #161514) were treated with 10 μg/ml blasticidin for selection of blasticidin and incubated for 72 hours ...
-
bioRxiv - Molecular Biology 2019Quote: ... CaCl2 transformation of JM83 cells obtained from Addgene with thermally denatured targeted DNA and ssODN was performed as described by Mandecki except for using SOC media instead of 2x YT for Ampr marker expression ...
-
bioRxiv - Cancer Biology 2019Quote: ... U2OS cells were transfected with pCBASceI (Addgene; #26477) in the presence of 1 µg/ml doxycycline ...
-
bioRxiv - Microbiology 2021Quote: ... 293T cells were co-transfected with psPAX2 (Addgene), pMD2.G VSV-g and the eSpCas9-LentiCRISPRv2 construct targeting ZMPSTE24 or the IFITM proteins or a non-targeting control at a ratio of 0.6:0.9:0.6 using 1 mg/ml PEI®-MAX ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells stably expressing inducible Snail (Addgene plasmid #18798) or indicated reporter plasmids were generated by transduction of LNCaP95 or CS2 cells as described:
-
bioRxiv - Genetics 2022Quote: DLD1 cells were transduced with 7TFC (Addgene#24307) lentivirus ...
-
bioRxiv - Molecular Biology 2022Quote: ... Jurkat cells containing pLenti-PE2-BSD (Addgene, #161514) were treated with 10 µg/ml blasticidin for selection of blasticidin and incubated for 72 hours ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were then transfected with plasmids (9021,60360,139059 - Addgene) using the Lipofectamine 3000 DNA Transfection Reagent for 48 h ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were transiently transfected with PX459 (Addgene; #48139) and selected with puromycin for 2 days ...
-
bioRxiv - Molecular Biology 2022Quote: ... cells were transduced with a pRDA_256 (Addgene #158581) co-expressing an sgRNA and BE3.9max with a puromycin resistance cassette ...
-
bioRxiv - Cancer Biology 2023Quote: ... cell membrane marker (pLenti-mCherry-CAAX, Addgene #129285), E-cadherin reporter (pHAGE-E-cadherin-RFP ...
-
bioRxiv - Microbiology 2023Quote: ... cells were transfected with pLew100cre-del-tetO (Addgene) [66] and selected with 40 µg/mL ganciclovir ...
-
bioRxiv - Cancer Biology 2023Quote: ... or GFP positive cells (when using Addgene #195124) were single cell sorted onto 96-well plates ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were infected with pRDA_186 plasmid (Addgene 133458), a gift from John Doench (Broad Institute) ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were transiently transfected with PX459 (Addgene; #48139) and selected with puromycin for 2 days ...