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Citations for Addgene :
251 - 300 of 1569 citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... CASP8 ORF was amplified from pcDNA3-CASP8 by PCR (Addgene #11817, a gift from Guy Salvesen) (Stennicke & Salvesen ...
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bioRxiv - Immunology 2022Quote: ... and Bach2 ORF (Origene, MR224703 cloned into Addgene, Cat# 52107, 1 μg/ml, marked by GFP) using the X-tremeGENE transfection reagent (Sigma Aldrich ...
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bioRxiv - Synthetic Biology 2022Quote: ... the 4272 bp ORF of Cas9 was amplified from pHsp70 Cas9 plasmid (Addgene plasmid #46294 (32)) using primers BR-666 and BR-667 and cloned into the vector between the zpg promoter and the 3’-UTR for zpg using BbsI for scarless ...
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bioRxiv - Microbiology 2020Quote: ... were constructed by replacing the Redβ open reading frame (ORF) of pORTMAGE311B plasmid (Addgene accession: 120418)43 with PapRecT and CspRecT respectively ...
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bioRxiv - Microbiology 2021Quote: ... The DNA sequence encoding the mCherry ORF was amplified from mCherry-H2A-10 (Addgene plasmid# 55054) by PCR using a forward primer harboring EcoR1 site (5’-TGACAGAATTCATGGTGAGCAAGG-3’ ...
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bioRxiv - Cell Biology 2020Quote: ... the fragment encoding the hL1CAM ORF was PCR amplified from the pcDNA3-hL1 plasmid (Addgene #89411) with primers Fwd ...
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bioRxiv - Biochemistry 2022Quote: ... PCR-amplified ORF inserts were Gibson assembled into NcoI-HF- and XhoI-digested pET-21a(+) (Addgene) and then transformed into E ...
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bioRxiv - Molecular Biology 2022Quote: ... reactions were used to move the PANK3 ORFs into the lentiviral expression vector pLEX_307 (Addgene #41392). Cells were transduced with lentivirus made from these vectors ...
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bioRxiv - Neuroscience 2024Quote: The ORFs of GFP and Ser42-GFP were cloned into the pAAV-CMV plasmid (Addgene #105530). Endofree plasmids were prepped with ZymoPURE Midiprep plasmid kit (Zymo Research ...
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bioRxiv - Immunology 2021Quote: ... myc (pCSF107mT-GATEWAY-3’-Myc tag, Addgene), green fluorescence protein (GFP ...
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bioRxiv - Cell Biology 2020Quote: ... or WIPI1 or WIPI2 cDNA were cloned with N-terminal EGFP or DsRed fusions in pCAG vector (a gift from Connie Cepko (Addgene plasmid #11150)) (Matsuda & Cepko ...
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bioRxiv - Cell Biology 2023Quote: ... a donor plasmid pAAVS1-TRE3G-NGN2 was generated by replacing the EGFP sequence with N-terminal flag-tagged human NGN2 cDNA sequence in plasmid pAAVS1-TRE3G-EGFP (Addgene plasmid # 52343). 5μg of pAAVS1-TRE3G-NGN2 ...
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Comprehensive fitness landscape of SARS-CoV-2 Mpro reveals insights into viral resistance mechanismsbioRxiv - Molecular Biology 2022Quote: ... The CyPet gene was amplified by PCR from the pCyPet-His vector (pCyPet-His was a gift from Patrick Daugherty; Addgene plasmid # 14030 ...
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Comprehensive fitness landscape of SARS-CoV-2 Mpro reveals insights into viral resistance mechanismsbioRxiv - Molecular Biology 2022Quote: ... The YPet gene was amplified by PCR from the pYPet-His vector (pYPet-His was a gift from Patrick Daugherty; Addgene plasmid # 14031 ...
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bioRxiv - Biophysics 2023Quote: ... The cDNA clones were amplified by polymerase chain reaction and subcloned into pLV-EF1a-IRES plasmid-blast vector (Addgene #85133), which we further engineered to introduce the HaloTag sequence for N-terminal tagging ...
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bioRxiv - Biochemistry 2021Quote: ... pFastBac1 His-MBP (#30116) were procured from Addgene. pNIC28-MBP was constructed by replacement of TrxT with MBP [22] ...
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bioRxiv - Bioengineering 2022Quote: ... the plasmid pET-28b-Cas9-His (Addgene #47327) was transformed into Rosetta (DE3 ...
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bioRxiv - Cancer Biology 2022Quote: ... FLT3-WT and FLT3-ITD ORFs were cloned in pLEX_307 (gift from David Root (Addgene plasmid #41392; http://n2t.net/addgene:41392 ; RRID:Addgene_41392))
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bioRxiv - Synthetic Biology 2022Quote: ... The 4272 bp ORF of Cas9 was then amplified from pHsp70 Cas9 plasmid (Addgene plasmid #46294 (32)) using primers BR-34 and BR-35 and inserted into the plasmid vector using NcoI and SalI ...
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bioRxiv - Molecular Biology 2022Quote: The GNB1L open reading frame (ORF) was inserted into the pLVU/GFP lentiviral plasmid vector (Addgene, 24177) encoding a C-terminal GFP tag ...
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bioRxiv - Microbiology 2022Quote: ... by replacing the RFP ORF in either pBbS8k-RFP or pBbE8k-RFP (Addgene Plasmids #35276 and #35270). For expression in B ...
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bioRxiv - Neuroscience 2024Quote: ... we amplified the FLP ORF from pCAG-Flpo (a gift from Dr. Massimo Scanziani; Addgene plasmid # 60662) with a AgeI restriction site on the 5’ end ...
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bioRxiv - Bioengineering 2024Quote: ... the MS2 coat protein (MCP) (N55K variant) ORF was PCR amplified from MS2-P65-HSF1_GFP (Addgene, 61423)74 and inserted with a serine-glycine linker via Gibson Assembly into pLV_CAG_Gag at the C-terminus of MLV Gag in order to generate the plasmid pLV_CAG_Gag-MCP ...
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bioRxiv - Bioengineering 2021Quote: ... the sequence encoding the PE2 N terminus (PE2-N term) (Addgene #132775) and a splicing donor (SD ...
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bioRxiv - Cell Biology 2020Quote: ... The mouse N-terminal Flag-tagged TRAF6 (Flag-TRAF6) mammalian expression plasmid was purchased from Addgene (#21624, GenBank: BAA12705.1). N-terminally GST-tagged TRAF6 (GST-TRAF6 ...
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bioRxiv - Cancer Biology 2021Quote: ... N-Myc (Addgene #74163) was cloned into plasmid pcDNA5/FRT/TO (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... and to link GFP11×7 tag (from Addgene Plasmid #70224 ...
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bioRxiv - Synthetic Biology 2020Quote: ... a C-terminal FLAG tag (pICSL50007, Addgene #50308), and a 3’ untranslated region and terminator sequence (3UTR ...
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bioRxiv - Molecular Biology 2023Quote: ... or a control MYC-mito tag (Addgene #83355) as previously described37 ...
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bioRxiv - Cancer Biology 2023Quote: ... to the MAC-Tag-C vector (Addgene #108077) [44] ...
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bioRxiv - Plant Biology 2023Quote: ... a C-terminal GR tag (pEPOZ0CM0137; Addgene #197535) and the CaMV35s terminator (pICH41414) ...
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bioRxiv - Molecular Biology 2024Quote: ... the FKBPv-V5 tag was obtained from Addgene plasmid 91798 and knocked into the Mlf2 and Smarca4 loci using HDR templates containing 0.5-1 kb homology arms flanking the C-terminal stop codons ...
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bioRxiv - Plant Biology 2024Quote: ... pICSL50009 (6xHA C-terminal tag, Addgene no. 50309), and pICSL60008 (Arabidopsis heat shock protein terminator (AtHSP18 terminator) ...
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bioRxiv - Bioengineering 2024Quote: Synthetic DNAs cording CDS of MAGTs obtained from GENEWIZ were fused with 3xMyc tag (pICSL50010; Addgene #50310) by Golden Gate cloning64 ...
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bioRxiv - Plant Biology 2024Quote: ... pICSL50007 (3xFLAG C-terminal tag, Addgene no. 50308), and pICH41414 (35S terminator ...
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bioRxiv - Plant Biology 2024Quote: ... pICSL50008 (GFP C-terminal tag, Addgene no. 50314), and pICH41414 (35S terminator).
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bioRxiv - Developmental Biology 2020Quote: Mouse YAP cDNAs were isolated by PCR amplification using DNA templates obtained from Addgene (Watertown, MA) and cloned into a shuttle vector at Creative-Biogene (Shirley ...
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bioRxiv - Molecular Biology 2021Quote: Mouse FLAG-Sp7 or osteocrin cDNAs were introduced via lentivirus via pLX_311 backbone (Addgene, plasmid 118018) as previously described [21] ...
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bioRxiv - Cell Biology 2024Quote: ... Mouse wild-type Pcyt2 cDNA (Horizon Discovery, MMM1013-202763346) was cloned into N174-MCS (Addgene, 81061) backbone digested with EcoRI and BamHI (New England Biolabs ...
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bioRxiv - Neuroscience 2023Quote: ... AAV9-hSyn- GrabDA1h (n = 5 Area X hemispheres; n = 2 MST hemispheres; Addgene), AAV5-CAG- Dlight1.1 (n = 1 Area X hemisphere ...
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bioRxiv - Cell Biology 2023Quote: ... The DNA fragment encoding SNAP-tag was amplified from the pSNAP-tag (m) vector (a gift from New England Biolabs & Ana Egana, Addgene #101135), and then inserted into the pDisplay vector (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR fragments were then subcloned into the pDEST-ORF-v1 (a kind gift from Darren Saunders, Addgene, #73637) (Croucher et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... SFPQ-EGFP was generated by subcloning the SFPQ ORF obtained from the Myc-PSF-WT plasmid (AddGene #35183). The FUS-EGFP plasmid was generated by inserting the FUS ORF into pcDNA6F-EGFP ...
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bioRxiv - Cancer Biology 2022Quote: ... CEBPA ORF was cloned in pINDUCER21 (ORF-EG) (gift from Stephen Elledge & Thomas Westbrook72; Addgene plasmid #46948; http://n2t.net/addgene: 46948 ; RRID:Addgene_46948). SCD ORF was cloned in pLenti PGK Puro DEST 5W(w529-2 ...
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bioRxiv - Genetics 2020Quote: ... The expression plasmid for human Nucleolin was constructed by amplifying the NCL ORF from GFP-Nucleolin (Addgene; #28176) using primers NCL-For and NCL-1XHA Rev (Table S1) ...
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bioRxiv - Synthetic Biology 2022Quote: ... The 4272 bp ORF of Cas9 was then amplified from the pHsp70 Cas9 plasmid (Addgene plasmid #46294 (32)) using primers BR-34 and BR-35 and inserted into pBluescript downstream of the nanos promoter fragment using a NcoI and SalI digest for the PCR product and a PciI/SalI digest for the plasmid vector ...
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bioRxiv - Cell Biology 2022Quote: We replaced the ORF of the GFP marker in the pSpCas9(BB)2A-GFP plasmid (Addgene plasmid #48138) by the ORF of iRFP670 ...
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bioRxiv - Plant Biology 2024Quote: ... The platinum TALEN ORFs designed to recognize the target sequences were assembled by platinum gate assembly kit (Addgene) (Sakuma et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... The CasX2 ORF was amplified from pBLO 62.5 (pBLO 62.5 was a gift from Jennifer Doudna and Benjamin Oakes, Addgene plasmid #123124 ...
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bioRxiv - Genomics 2023Quote: ... was used to amplify all of the open reading frames (ORFs) from their original vectors (Addgene or ORFeome) in order to add attB sites ...