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Citations for Addgene :
251 - 300 of 301 citations for PEG NH2 modified upconverting red light since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: A lentiviral vector suitable for convenient subcloning between these modified pMY and pMX vectors was made by modifying lentiCRISPR v2 (Addgene: #52961) (Sanjana ...
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bioRxiv - Biophysics 2020Quote: ... The PCR product containing codon optimized nsp12 was cloned into the modified pFastBac vector 438-C (a gift from S. Gradia, UC Berkeley, Addgene: 55220) via LIC ...
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bioRxiv - Biochemistry 2022Quote: ... expression plasmid was cloned by PCR amplification from full length ScTop2 12URA-B vector followed by insertion into a modified 1-B (Addgene #29653) E ...
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bioRxiv - Cell Biology 2022Quote: ... gRNA or a tandem cassette of 3 gRNAs targeting FBXL4 was cloned into AAV-U6-gRNA-CAG-mtKeima-WPRE-hGHpA (modified from Addgene, 60229) under the U6 promoter ...
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bioRxiv - Neuroscience 2022Quote: ... The reference was generated from the mouse genome (GRCm38) and annotation (GRCm38.93) and modified to include the sequences for tdTomato (Addgene plasmid # 22799) (Madisen et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... The annealed oligo products were directionally cloned into a modified version of the gRNA+LbCas12a expression plasmid pTE4398 (Addgene, Watertown, MA) as crRNA genes for sgRNA expression ...
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bioRxiv - Molecular Biology 2022Quote: ... The fragments are then cloned into a mammalian expression vector containing Flag and mEGFP (N- or C-terminal) (modified from Addgene #32104) using NEBuilder HiFi DNA Assembly kit (E2611) ...
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bioRxiv - Neuroscience 2024Quote: The pAAV-CMV:Cas9-T2A-mCherry/U6:sgRNA plasmid was modified from pX601 (pAAV-CMV:NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA; Addgene plasmid # 61591). To visualize the AAV-infected cells ...
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bioRxiv - Cell Biology 2024Quote: ... MEFs and MDA-MB-231 cells were stably modified using BFP and DN-KASH expression plasmids in a doxycycline-inducible Piggybac plasmid backbone (Addgene #187019). For lentiviral modifications ...
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bioRxiv - Molecular Biology 2023Quote: ... Due to the Purmocyin resistance the XEN-dCas9-BFP-KRAB cells were infected with a modified version of the pLKO5.GRNA.EFS.PAC vector (Addgene, cat. no. 57825) replacing puromycin with blasticidin resistance ...
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bioRxiv - Neuroscience 2023Quote: ... The plasmids used in this study are modified from the pSox2-bd::FP construct (Addgene plasmid #34703; Bestman et al., 2015), a plasmid that contains a weak FGF4 promotor regulated by 6 repeats of the Sox2 transcription factor binding domain ...
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bioRxiv - Genomics 2023Quote: sgRNAs targeting 3 different locations in the genome were cloned into a modified version of pU6-sgRNA EF1Alpha-puro-T2A-BFP (Addgene, #60955), where BFP was replaced by superfolder GFP (sfGFP ...
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bioRxiv - Neuroscience 2023Quote: ... using in house modified versions of hygromycin-resistant and MiniMos enabled vector pCFJ1662 (gift of Erik Jorgensen, University of Utah, Addgene #51482): pCFJ1662 Phyp7 mNeonGreen GTWY let858 (34B2) ...
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bioRxiv - Cancer Biology 2023Quote: ... pX330 was sequentially modified to accept a 2.4kb Cas9-P2A-Cre fragment from pSECC (a gift from Tyler Jacks (Addgene plasmid # 60820) first by an EcoRI (New England Biolabs ...
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bioRxiv - Neuroscience 2023Quote: ... The 40% iodixanol layer after ultracentrifugation was collected and buffer exchanged with DPBS using Amicon Ultra centrifugal filters (#Z648043, Millipore) (modified protocol from Addgene, USA). We performed quantitative PCR on the viral stocks and the titer was determined as approximately 1.9 x 1012 genomic copies/ml for S5E2-lox dTom lox rcGFP lox2 and 1.1 x 1012 genomic copies/ml for S5E2-lox ChR2 mCh lox.
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bioRxiv - Cancer Biology 2024Quote: sgRNA sequences targeting KSR1 or non-targeting control (NTC) were inserted into TLCV2-RB1 (pLentiCRISPRv2GFP modified with puromycin selection marker) (Addgene #87836). The constructs were PEI transfected into HEK293T cells along with psPAX2 lentiviral packaging construct (Addgene #12259 ...
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bioRxiv - Molecular Biology 2024Quote: ... before being cloned into the BsmBI site (vectors had been modified to contain this site) of either the constitutive lentiviral vector FUGW (Addgene #14883) or the doxycycline-inducible vector FgH1tUTG (Addgene #70183) ...
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bioRxiv - Neuroscience 2024Quote: ... This plasmid was transfected into iPSCs stably expressing the SunTag scFv tagged with HaloTag under an Ef1alpha promoter (modified from Addgene 60907), PP7 coat protein tagged to 3 SnapTags (synthesized) ...
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bioRxiv - Neuroscience 2024Quote: TDP-43 coding sequence and 3’UTR were cloned into a plasmid expressing 24 SunTag repeats under an Ef1alpha promoter followed by 24 PP7 stem loops32 (modified from Addgene 74928). This plasmid was transfected into iPSCs stably expressing the SunTag scFv tagged with HaloTag under an Ef1alpha promoter (modified from Addgene 60907) ...
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bioRxiv - Cell Biology 2024Quote: ... The first plasmid encodes the inserted sequence flanked by two microhomology arms with a length of 40 bp and containing short PITCh sequences at their distal ends (modified based on pCRIS-PITChv2-FBL, Addgene #63672) (Sakuma et al. ...
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bioRxiv - Genetics 2024Quote: ... with an HA-tag sequence fused to its 5’ end was cloned into a PiggyBac vector (pPB-CAG-FLAG-HA, a modified version of pPB-CAG-3xFLAG-empty-pgk-hph from Addgene, #48754) to create pPB-CAG-FLAG-HA-ZNF263 plasmid via Gibson assembly (NEB ...
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bioRxiv - Genetics 2020Quote: ... with 20 ng/μl of dg9 (unc-122p::RFP; red coelomocyte) as a co-injection marker (Addgene #8938; Miyabayashi et al. 1999). Two stable lines of each candidate promoter were selected (Table S1) ...
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bioRxiv - Synthetic Biology 2023Quote: The genes gabT and gabP were deleted from the EcN genome using the pSIJ8 plasmid (Jensen et al. 2016) containing the lambda Red recombineering system and ampicillin resistance (Addgene plasmid #68122). The plasmid was transformed into EcN via electroporation ...
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bioRxiv - Microbiology 2020Quote: ... The resulting amplicons and gene fragment were then cloned into a modified pLenti CMV GFP Puro plasmid (a gift from Eric Campeau & Paul Kaufman; Addgene plasmid #17448), which contains a 3’ WPRE sequence following the insert and a 3’ SV40 polyadenylation signal after the puromycin resistance cassette ...
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bioRxiv - Cell Biology 2020Quote: To generate the ATF4-SunTag reporter, we have modified the previously-described SunTag-Renilla-MS2 plasmid (Wilbertz et al., 2019) (Addgene plasmid #119945) by inserting the cDNA of human ATF4 5’UTR (until the end of uORF2 ...
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bioRxiv - Molecular Biology 2021Quote: ... ΔIDR-SHARP) were then recombined into two different modified versions of the doxycycline inducible PiggyBac destination vector PB-TAG-ERN (Addgene plasmid 80476) containing NGFR (truncated human nerve growth factor receptor ...
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bioRxiv - Molecular Biology 2021Quote: ... was randomly integrated in the genome of the target cell line via lentiviral transduction of a modified version of pHR-SFFV-dCas9-BFP-KRAB (Addgene plasmid # 46911) carrying a P2A blasticidin selection and UCOE element (kind gift of Marvin Tanenbaum) ...
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bioRxiv - Developmental Biology 2020Quote: ... specific gRNAs targeting the catalytic center of Tet1 and Tet2 were cloned into a modified version of the SpCas9-T2A-GFP/gRNA (px458116, Addgene plasmid #48138), to which we fused a truncated form of human Geminin (hGem ...
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bioRxiv - Bioengineering 2020Quote: ... The construction of PEgRNA transcript carrying plasmid: The empty PEgRNA plasmid was modified from the pgRNA-bacteria (ColE1 ori, Addgene plasmid #44251)30 by removing the 20 bp spacer ...
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bioRxiv - Biophysics 2021Quote: ... in fibril gene were mutated to TGG and full-length fibril gene with modified tryptophan codons was cloned into pHIS17 vector (Addgene plasmid #78201) with or without a terminal hexa-histidine (His6 ...
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bioRxiv - Neuroscience 2022Quote: ... Guide sequence with minimal off target and very high activity score was chosen and cloned into the BbsI restriction site of the Cas9 plasmid (Cas9-P2A-Puro modified from Addgene #62988(30)) ...
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bioRxiv - Microbiology 2021Quote: ... Michèle Bouloy (Institut Pasteur, France) were induced to overexpress ACE2 using a modified lentiviral expression system (pLV-EF1a-IRES-Neo (Addgene, plasmid 85139)) ...
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bioRxiv - Genomics 2022Quote: ... In order to integrate CRISPR targets and sgRNAs into the genome, we modified the CROPseq vector (Datlinger et al., 2017) (Addgene ID 86708), which expresses an sgRNA and a PolII transcript ...
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bioRxiv - Developmental Biology 2021Quote: ... specific gRNAs targeting upstream of the stop codon (Supplementary Table) were cloned into a modified version of the SpCas9-T2A-GFP/gRNA plasmid (px458172, Addgene plasmid #48138), where we fused a truncated form of human Geminin (hGem ...
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bioRxiv - Developmental Biology 2021Quote: ... specific gRNAs targeting upstream of the stop codon (Supplementary Table) were cloned into a modified version of the SpCas9-T2A-GFP/gRNA plasmid (px458172, Addgene plasmid #48138), where we fused a truncated form of human Geminin (hGem ...
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bioRxiv - Cell Biology 2020Quote: ... shRNA containing vectors were constructed by ligation of shRNA sequence between the EcoRI and PmeI sites downstream of the U6 promoter of modified pEGFP-U6 vector 36 (a gift from Zuoshang Xu, Addgene plasmid #19799). Target sequence for human MARCKSL1 is 5’-GTGTGAACGGAACAGATGATG-3’ ...
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bioRxiv - Immunology 2021Quote: ... The S106C mutant of human ASC PYD (aa1-106) was cloned into an in-house modified pET28-MBP-TEV vector (Addgene, plasmid #69929), in which N-terminal His6 tag was added for expression of proteins with a cleavable N-terminal His6-MBP-tag ...
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bioRxiv - Cell Biology 2022Quote: ... were constructed by cloning the open reading frame (ORF) of human IRF1 or cMYC into a modified pLX303 vector (Addgene plasmid 25897). cDNAs encoding IRF1-SBDmut ...
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bioRxiv - Cell Biology 2022Quote: ... The TTP stability reporter (pLX-SFFV-mCherry-TTP-P2A-BFP) was constructed by cloning the open reading frame (ORF) of murine TTP into a modified pLX303 vector (Addgene plasmid 25897). Lentiviral N-terminally HA-tagged-TTP deletions or point mutant variants were obtained by cloning the indicated variants of murine TTP ORF into a modified pLX303 vector ...
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bioRxiv - Neuroscience 2022Quote: ... placed upstream of GFP for histology and gene expression experiments, and upstream of GCaMP7f (Dana et al., 2019) (modified from Addgene plasmid #104495), or a conditional version of GCaMP8s (Zhang et al. ...
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bioRxiv - Neuroscience 2022Quote: The CRISPRi lentivirus construct was modified from pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP (Addgene #71237, a gift from Charles Gersbach28), which expresses all necessary CRISPRi machinery (both the dCas9-KRAB and sgRNA ...
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bioRxiv - Microbiology 2023Quote: ... Two gRNA binding sites near the 3’ region of each gene of interest were identified using EuPaGDT Editing of the previously modified pTREX-n-Cas9 plasmid 51 (Addgene plasmid 68708), performed to exchange the previous gRNA sequence was achieved using a Q5 mutagenesis kit (New England Biolabs ...
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bioRxiv - Genetics 2024Quote: ... and the resulting insert gel purified as above and ligated into the modified pcAT7-Glo1 vector (a gift from Brenton Graveley, Addgene Plasmid # 160996) prepared with NdeI and BglII digest and gel purification ...
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bioRxiv - Biophysics 2024Quote: ... a 29 nt long Cy5 oligo was employed to produce a 1.3 kbp dsDNA via PCR from a modified pQE-UBE1 plasmid (Addgene, Watertown, MA, USA). The PCR product was purified through ethanol precipitation ...
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bioRxiv - Genetics 2020Quote: ... targeting upstream and downstream of LTRs of interest were annealed and cloned into modified eSpCas9 (1.1) vector (Addgene 71814, deposited by Feng Zhang), which expresses GFP ...
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bioRxiv - Microbiology 2021Quote: ... Guide RNAs were cloned into the pX330-U6-Chimeric_BB-CBh-hSpCas9 plasmid backbone using a modified version of the Zhang Lab General Cloning Protocol (Addgene http://www.addgene.org/crispr/zhang/). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNAs were used as PCR templates for cloning into modified pFastBac vectors (derivatives of 438-A and 438-C; Addgene 55218 and 55220) by ligation independent cloning (LIC)37 ...
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bioRxiv - Neuroscience 2022Quote: ... mice were first injected with a helper virus (AAV5- hSyn-FLEX-splitTVA-2A-mCherry-2A-B19G; produced in-house, modified after Addgene plasmid no. 52473) into MEC ...
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bioRxiv - Genetics 2022Quote: ... The resulting modified LexA (mLexA) chimeras were combined through DNA assembly (Gibson et al. 2009) with the following components: eLOV (Addgene # 92213, Alice Ting lab) (Wang et al ...
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bioRxiv - Molecular Biology 2023Quote: The MCS of pBAD24 (Guzman et al., 1995) was exchanged for the red fluorescent protein (RFP) cassette from pPGC-C (Addgene plasmid # 174580,(Bentham et al., 2021)) flanked by BsaI restriction sites ...