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Citations for Addgene :
151 - 200 of 301 citations for PEG NH2 modified upconverting red light since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: Plasmid encoding the modified AAV capsid of interest were subcloned into tTA-iCAP-PHP.eB (Addgene). The mutated version of Se1 and Se2 were generated using as donor sequence the clones derived from the third round of selection which contained the heptapeptide at position 589 and the W503A mutation (VP1 numbering) ...
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bioRxiv - Cell Biology 2024Quote: ... and Cas9 (modified based on pX330A-1x2, Addgene #58766, and pX330S-2-PITCh, Addgene #63670) (Sakuma et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... A modified version of the PE2 plasmid by the lab of David Liu (Addgene, #132775) containing PE2 coupled via a P2A peptide to a puromycin resistance cassette was kindly provided by the lab of Susanne Lens (University Medical Center Utrecht ...
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bioRxiv - Cell Biology 2020Quote: ... pLVTHM and pLV-tTR-KRAB-red were gifts from Didier Trono (Addgene plasmids # 12247 and # 12250).
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bioRxiv - Cell Biology 2022Quote: ... mKeima-Red-Mito-7 plasmid was a gift from Michael Davidson (Addgene plasmid #56018; www.addgene.org/56018). MitoTracker Deep Red FM ...
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bioRxiv - Bioengineering 2024Quote: ... The mCherry red fluorescent protein was taken from the pU6-pegRNA-GG-acceptor plasmid (Addgene #132777) via PCR using Q5 High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Developmental Biology 2020Quote: ... Snrpb and Snrpd2 cDNA were cloned into a modified pCS2+8NmCherry vector lacking mCherry tag (Addgene) for their in vitro transcription ...
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bioRxiv - Microbiology 2021Quote: ... two constructs (Fig. 1A) were made using a modified pgRNA-bacteria plasmid (NEB, Addgene plasmid # 44251). This plasmid ...
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Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying essential circRNAsbioRxiv - Molecular Biology 2020Quote: ... the cells were transduced by EF1a-CasRx (no NLS-RfxCas13d)-2A-EGFP (modified from Addgene #109049) lentivirus ...
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bioRxiv - Synthetic Biology 2022Quote: The original Cas9 and gRNA expression modules were modified from p414-TEF1p-Cas9-CYC1t (Addgene# 43802) and p426-SNR52p-gRNA.CAN1.Y-SUP4t (Addgene# 43803) ...
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bioRxiv - Immunology 2024Quote: Lentiviral transfer plasmids encoding different transgenes were constructed into the pHR vector (modified from Addgene #79125) by NEBuilder HiFi DNA Assembly (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... EGFP-MISP sequence was subcloned into modified pFastBac-6xHis-MBP LIC expression vector (Addgene; plasmid #30116). In-frame sequence insertion was confirmed by sequencing ...
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bioRxiv - Neuroscience 2023Quote: ... Addgene #55639 (packaged in AAV2/1) AAV2/8-EF1a-fDIO-ChrimsonR-mRuby2-KV2.1TS modified from Addgene #124603 pAAV-hSyn1-SIO-stGtACR2-FusionRed ...
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bioRxiv - Cancer Biology 2024Quote: ... sgRNAs were cloned into a modified U6-based expression vector pSLQ1651-sgTelomere (F+E) (Addgene #51024) that enhanced sgRNA stability and assembly with dCas9 protein64 ...
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bioRxiv - Biophysics 2024Quote: ... and inserted into the SspI site of the 2B-T modified pET vector (Addgene plasmid #29666) using the ligation-independent cloning protocol ...
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bioRxiv - Genomics 2024Quote: ... and cloned amplified oligos into a modified pMPRA1 vector (a gift from Tarjei Mikkelsen; Addgene #49349) using Golden Gate assembly with PaqCI ...
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bioRxiv - Microbiology 2022Quote: ... The helper plasmid pSIJ8 expressing the λ Red and FLP recombinase was obtained from Addgene (cat. 68122). pSIJ8 can replicate at 30 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... we generated PCR amplified EF1a promoter and TetR-NLS signal cassette from pLV-tTRKRAB-red (Addgene, #12250). 3xFlag-KRAB was PCR amplified from the same vector with a forward primer including the sequence encoding 3xFlag and SV40pA amplified from lentiCRIPSRv2 ...
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bioRxiv - Developmental Biology 2024Quote: ... mEos3.2 and green-to-red photoconvertible protein (a gift from Michael Davidson & Tao Xu (Addgene plasmid 54525) was coupled to Histone 2B (H2B ...
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bioRxiv - Neuroscience 2023Quote: ... Tetanus Neurotoxin Light Chain (TeLC) viruses were generated using AAV5-hSyn-FLEX-TeLC-P2A-dTomato (Addgene, 159102, 1 × 1013 genome copies per ml) at ISTA viral facility ...
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bioRxiv - Plant Biology 2021Quote: CRISPR/Cas9 knockout mutants in MIR823 were created by using a modified pHSE401 binary vector (Addgene #62201) according to the protocol detailed by (Xing et al. ...
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bioRxiv - Neuroscience 2021Quote: To express constructs by in Utero electroporation we obtained and modified the following plasmid constructs from Addgene: CAG-tdTomato ...
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bioRxiv - Cancer Biology 2022Quote: ... The modified CROP-seq-MS2 plasmid was obtained as following: the lentiviral CROP-seq-opti vector (Addgene plasmid #106280 was a gift from Jay Shendure ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNAs for Decorin and E-cadherin (Gene ID: 999) were cloned into modified pHA-N1 (#86049, Addgene) and pcDNA3-neo-Strep-flag-Cterm (#102645 ...
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bioRxiv - Biochemistry 2021Quote: ... This vector was modified from 3xHA-TurboID-NLS_pCDNA3 which was a gift from Alice Ting (Addgene # 107171).
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bioRxiv - Neuroscience 2024Quote: The pAAV-pMeCP2:DIO-Cas9 plasmid was modified from pX551 (pAAV-pMeCP2:SpCas9-spA; Addgene plasmid # 60957) and was gifted by Dr ...
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bioRxiv - Cell Biology 2024Quote: ... The pCAG-i53bp expression plasmid was a gift from Ralf Kuhn and was modified from Addgene (RRID:Addgene_74939). The SV40-NLS-CRE recombinase was a gift from Christoph Harms and was subcloned into the pLenti6 backbone ...
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bioRxiv - Neuroscience 2023Quote: ... we subcloned a 33.4 kb fragment from the modified BAC into pMCS-DTA (Addgene plasmid #22730; (65) to generate a targeting vector which extends from 4.9 kb upstream of the loxrox-Hygro/Zeo insertion to 5 kb downstream of the 2ATNSAG insertion.
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bioRxiv - Bioengineering 2023Quote: ... ssDNA binding protein and peptide sequences were synthesized and assembled into an AIO plasmid modified from Addgene plasmid #42230 by golden gate cloning ...
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A CRISPR activation screen identifies FBXO22 as an E3 ligase supporting targeted protein degradationbioRxiv - Biochemistry 2023Quote: ... FKBP12-EGFP construct was generated by cloning FKBP12 gene into a modified Artichoke plasmid (Addgene plasmid#: 73320) in which the selection marker puromycin was replaced with neomycin ...
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bioRxiv - Genomics 2023Quote: ... then cloned the amplified fragments into a modified pMPRA1 vector (a gift from Tarjei Mikkelsen; Addgene #49349) using Golden Gate assembly with PaqCI ...
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bioRxiv - Cell Biology 2024Quote: ... and the downstream guide was cloned into a modified version of the pLentiGuide-Puro vector (Addgene, #52963), in which the puromycin selection marker was replaced with a hygromycin selection marker.
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bioRxiv - Neuroscience 2024Quote: ... and beat-VI were cloned into a modified pJFRC5 vector (Addgene: 5XUAS-IVS-mCD8::GFP, plasmid #26218) by replacing the mCD8::GFP coding sequence ...
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bioRxiv - Synthetic Biology 2024Quote: ... CrRNA eukaryotic expression vectors were ligated by primer annealing into the modified Gs12 scaffold from Addgene (#67975) in the vector pLenti-U6-Gs12-crRNA-zsGreen ...
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bioRxiv - Bioengineering 2024Quote: ... The CAG-EGFP-WPRE-hGH polyA signal was modified by removing the bGH polyA signal from Addgene #37825 (gift from Ed Boyden ...
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bioRxiv - Molecular Biology 2020Quote: ... pLV-tTRKRAB-red and pLVTHM were a gift from Didier Trono (Addgene plasmid # 12250; http://n2t.net/addgene:12250; RRID:Addgene_12250 and Addgene plasmid # 12247 ...
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bioRxiv - Bioengineering 2024Quote: oROS-HT variants were cloned based on the pC1 plasmid backbone from pC1-HyPer-Red (Addgene ID: 48249). Primers for point mutations or fragment assembly required to generate the oROS-HT screening variants were designed for In Vitro Assembly cloning (IVA ...
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bioRxiv - Neuroscience 2023Quote: ... mKeima-Red-Mito-7 was a gift from Michael Davidson (Addgene plasmid # 56018; http://n2t.net/addgene:56018; RRID:Addgene_56018) Transfections have been performed with Lipofectamin 3000 (Thermofisher ...
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bioRxiv - Bioengineering 2022Quote: ... we cloned new variant plasmids by digesting a modified version of the pUCmini-iCAP-PHP.eB (Addgene ID: 103005) backbone using MscI and AgeI ...
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bioRxiv - Biophysics 2022Quote: ... We cloned the single-guide RNAs (sgRNAs) targeting the LTR-MS2 reporter into a modified pLCKO (Addgene #73311) to co-express guide RNAs from a hU6 promoter and mCherry from an hPGK promoter ...
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bioRxiv - Synthetic Biology 2022Quote: ... This was modified by adding AarI restriction sites and a lacZ cassette for selection producing pEPQD0KN0750 (Addgene#185627). Spacer sequences for selected targets were incorporated into pEPQD0KN0750 by Golden Gate assembly into AarI sites as previously described55 (Supplementary Table S7) ...
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bioRxiv - Systems Biology 2022Quote: ... The optoSOS construct is a modified version of the tRFP-SSPB-SOScat-P2A-iLID-CAAX (Addgene plasmid #86439) (Johnson et al ...
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bioRxiv - Cancer Biology 2021Quote: All human and mouse melanoma lines were engineered to overexpress Cre under the UBC promoter modified from Addgene plasmid #65727 as described in87 ...
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bioRxiv - Cancer Biology 2022Quote: ... sgRNA#2: 5’-aacctgagtgatatgactag-3’) were cloned into a modified version of the lentiCRISPR v2 backbone (RRID: Addgene_52961) in which a puromycin resistance ORF was cloned under the hPGK promoter ...
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bioRxiv - Molecular Biology 2022Quote: ... coli were constructed via an intermediate plasmid pCas9SX derived by replacing the lambda red ORF of pCasPA (Addgene #113347) (Chen et al. ...
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bioRxiv - Neuroscience 2022Quote: ... we injected either rAAV2/10 encoding ChrimsonR(K176R) and the red fluorophore tdTomato under control of the human synapsin promoter (1.13 × 1013 gc/ml; AddGene plasmid #59171 ...
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bioRxiv - Bioengineering 2024Quote: oROS-HT variants were all cloned based on the pC1 plasmid backbone from pC1-HyPer-Red (Addgene ID: 48249). Primers for point mutations or fragment assembly required to generate the oROS-HT screening variants were designed for In Vitro Assembly cloning (IVA ...
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bioRxiv - Developmental Biology 2020Quote: The modified PiggyBac-GA-CRISPRa vector (pW210) was generated by replacing the Zeocin resistance cassette of pSLQ2842 (Addgene, 84244) with a synthesized Hygromycin resistance cassette (IDT ...
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bioRxiv - Cancer Biology 2020Quote: We generated a lentiviral vector named CRISPRi-Puro (modified from pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro; Addgene Plasmid #71236 ...
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bioRxiv - Neuroscience 2021Quote: ... and inserted a strong CAG promoter (CMV immediate early enhancer/modified chicken β-actin promoter, from Addgene Plasmid #1378) in front of the FLEX-cassette to create pRMCE-CAG-Flex ...