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Citations for Addgene :
51 - 100 of 301 citations for PEG NH2 modified upconverting red light since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... we modified one lentivirus EGFP reporter plasmid (Addgene, #137725) by replacing the scaffold-attached region (SAR)61,62 with human anti-repressor element 4063 ...
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bioRxiv - Molecular Biology 2022Quote: PegRNAs-expressing plasmids (pU6-pegRNA) were cloned by ligating annealed oligo pairs (Supplemental table 2) with BsaI-digested pU6-peg-GG-acceptor (Addgene #132777) as described previously (Anzalone et al. ...
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bioRxiv - Cell Biology 2023Quote: ... The GFP-α-tubulin coding sequence was cloned into the pEG BacMam vector (a gift from Eric Gouaux, Addgene plasmid #160451), recombinant bacmid DNA was generated in DH10Bac cells (Thermo Fisher 10361012) ...
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bioRxiv - Molecular Biology 2022Quote: cDNA encoding RNaseH1-GFP was amplified from RED (Addgene # 139835) vector using the primers below ...
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bioRxiv - Cell Biology 2023Quote: ... a monomeric red fluorescent protein (RFP)-FKBP12 (Addgene, Plasmid #67514) (71 ...
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bioRxiv - Biophysics 2023Quote: ... was cloned into the pEG-BacMam expression vector with the GFP and 8X-His tags at the C-terminus (Addgene: Table S2). All constructs with large domain insertions and deletions were made using standard protocols for Gibson assembly (New England Biolabs ...
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bioRxiv - Cancer Biology 2021Quote: ... and ligated into a modified pBabe-Puro retroviral backbone (Addgene plasmid #1764 modified to insert FseI and AscI between BamHI and EcoRI within the MCS ...
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bioRxiv - Molecular Biology 2021Quote: ... DsRed plasmid was modified from pLenti-DsRed_IRES_EGFP (Addgene #92194, ref19) by deleting IRES-EGFP ...
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bioRxiv - Immunology 2021Quote: ... then into modified pLEX307 vectors originating from pLEX307 (Addgene #41392) using Gateway cloning technology (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... we modified two plasmids procured from Addgene (#62724 and #74291). We digested plasmid #74291 with BamHI ...
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bioRxiv - Neuroscience 2021Quote: ... Kim modified two AAV vectors (Addgene plasmid #18917 and #38044). DNA of #18917 was cut with BamHI and Eco RI ...
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bioRxiv - Developmental Biology 2022Quote: ... gRNAs then were cloned into modified pL-CRISPR.EFS.GFP construct (RRID:Addgene_57818) with removal of Cas9 ...
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bioRxiv - Molecular Biology 2021Quote: ... or pSpCas9(BB)-2A-RFP (modified from Addgene plasmid #48138) as described previously67 ...
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bioRxiv - Developmental Biology 2023Quote: ... modified from pPyCAG-Cre::ERT2-IRES-BSD (Addgene plasmid #48760) by changing blasticidin-resistance gene to puromycin-resistance gene ...
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A CRISPR activation screen identifies FBXO22 as an E3 ligase supporting targeted protein degradationbioRxiv - Biochemistry 2023Quote: ... CRISPRa vector was modified from LentiGuide-Puro (Addgene plasmid#: 52963) by inserting a tetraloop and stem loop sequences after the BsmBI restriction site18 ...
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bioRxiv - Cell Biology 2021Quote: ... mKeima was amplified by pcr using mKeima-RED-N1 (Addgene #54597) as a template ...
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bioRxiv - Neuroscience 2023Quote: ... mKeima-Red-Mito-7 was a gift from Michael Davidson (Addgene plasmid # 56018 ...
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bioRxiv - Biophysics 2023Quote: ... and its variants were cloned into the pEG-BacMam expression vector with GFP and 8X-His tags at the N-terminus (Addgene, see Table S2). For FLAG-tagged KRAS expression constructs ...
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bioRxiv - Microbiology 2020Quote: ... we modified the pSAG1::Cas9-U6::sgUPRT (Addgene plasmid # 54467; [39]) vector using Q5 mutagenesis (New England Biolabs ...
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bioRxiv - Neuroscience 2020Quote: ... 0.35 μg Cas9 plasmid (Cas9-P2A-Puro modified from Addgene #62988) containing a gRNA sequence targeting the stop codon of the PDGFRα locus and 0.75 μg of donor plasmid were used for transfection ...
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bioRxiv - Cell Biology 2020Quote: ... cDNAs were cloned into a modified pCW57.1 lentiviral vector (Addgene #41393) generated within the ReSOLUTE consortium and carrying a Strep-HA tag and blasticidin resistance.
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bioRxiv - Developmental Biology 2022Quote: Cas9/AAVS1 gRNA-modified pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene Plasmid #62988 [44] with T2A replaced with P2A ...
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bioRxiv - Molecular Biology 2022Quote: ... constructs were inserted into a modified version of pSMPP (Addgene #104970) where the SFFV promotor and puromycin resistance sequences were replaced with PGK1 promoter and Zeocin resistance sequences respectively (pPMEZ) ...
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bioRxiv - Cancer Biology 2020Quote: ... The sgRNA was cloned into the modified pX330 (Addgene, Cat# 42230) construct co-expressing wild type SpCas9 and a PGK-Venus cassette ...
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bioRxiv - Genomics 2021Quote: ... we modified pMK243 (Tet-OsTIR1-PURO) from Masato Kanemaki (Addgene #72835). pMK243 was digested by BgIII and MluI to remove OsTIR sequence ...
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bioRxiv - Cell Biology 2020Quote: ... a previously modified version [23] of the pEGFP-N2 vector (Addgene). Mutations were introduced into the GTSE1 cDNA by either site-directed mutagenesis or Gibson cloning ...
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bioRxiv - Genetics 2020Quote: ... The AAVS1 targeting fluorescent reporter #208 system was modified from Addgene ID 60431 ...
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bioRxiv - Cancer Biology 2021Quote: CDK2 reporter DHB-iRFP was modified from DHB-Venus (Addgene #136461)[46] ...
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bioRxiv - Immunology 2023Quote: ... The shRNAs were cloned separately into LentiCRISPRv2-GFP (modified from Addgene) and transiently co-transfected with psPAX2 (12260 ...
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bioRxiv - Molecular Biology 2023Quote: ... we modified constructs of the Kanemaki lab (Addgene #72827 and #121180). In order to incorporate mRuby2 ...
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bioRxiv - Cell Biology 2022Quote: ... a modified version of the pX459 V2.0 vector (62988, Addgene plasmid) carrying an optimized gRNA scaffold ...
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bioRxiv - Genetics 2023Quote: ... The backbone plasmid was modified from plasmid LentiGuide-Puro (Addgene, 52963), with an addition of a CMV promoter ...
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bioRxiv - Microbiology 2023Quote: ... in the target plasmid pMB2a-tet (a modified version of Addgene number ...
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bioRxiv - Developmental Biology 2023Quote: ... cloned into a pBID-UBI plasmid (modified from Addgene plasmid #35200), transformed into NEB 5-alpha competent cells ...
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bioRxiv - Cell Biology 2024Quote: ... which was previously modified from construct mCherry-CRY2PHR (pHR) (Addgene #201750) by introducing two restriction enzyme sites BstZ17I and BsiWI ...
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bioRxiv - Molecular Biology 2020Quote: ... pLV-tTRKRAB-red and pLVTHM were a gift from Didier Trono (Addgene plasmid # 12250 ...
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bioRxiv - Plant Biology 2021Quote: ... It was then assembled with a FAST-Red selectable marker (Addgene: 117499) and the sgRNA level 1 cassette into a level 2 vector pICSL4723 ...
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bioRxiv - Bioengineering 2021Quote: ... 32] was genetically fused to Red enhanced Nano-lantern (ReNL [30]; Addgene plasmid #89536 ...
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bioRxiv - Developmental Biology 2022Quote: ... and cloned into a pBID-Ubi plasmid (modified from Addgene Plasmid #35200) or were directly synthesized into the desired pBID-Ubi plasmid cloning site (Twist Bioscience ...
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bioRxiv - Synthetic Biology 2021Quote: ... a modified version of pX459 (Addgene #62988, a gift from Feng Zhang) that has the Puromycin resistance gene replaced by a Blasticidin resistance gene ...
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bioRxiv - Cell Biology 2021Quote: ... SOX2-mCherry construct was modified from SOX2-t2a-mCherry (Plasmid #127538, Addgene) by replacing fragments of the 3’ homology arm to target the UTR region of SOX2 after stop codon using gene synthesis (Eurofins genomics ...
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bioRxiv - Genetics 2022Quote: We then modified the original CRISPR-Swap plasmid (PFA0055, Addgene plasmid #131774) to replace its LEU2 selectable marker with the HIS3 selectable marker ...
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bioRxiv - Biophysics 2022Quote: ... The transfer vector consisted of a modified pLKO.1 neo plasmid (Addgene) with expression of the shRNA sequences under control of 3× copies of the lac operator and a copy of the mNeonGreen fluorescence protein ...
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bioRxiv - Molecular Biology 2020Quote: ... coding sequences were inserted into a modified version of pSMPP (Addgene #104970) where the SFFV promotor and puromycin resistance sequences were replaced with PGK1 promoter and Zeocin resistance sequences respectively (pPMEZ ...
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bioRxiv - Bioengineering 2021Quote: ... The Rep-Cap plasmid for packaging AAV.FUS candidates were modified from Addgene plasmid #103005 by introducing mutations selected from the screen ...
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bioRxiv - Cell Biology 2021Quote: ... Lentiviral plasmids were generated based on a modified Puro-Cre vector (Addgene plasmid # 17408 ...
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bioRxiv - Synthetic Biology 2022Quote: ... DHY213 was first modified by integrating the linearized FRP2370 plasmid (Addgene #127576), which encodes a cassette expressing the Tet repressor ...
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bioRxiv - Synthetic Biology 2024Quote: ... which was modified by our laboratory in previous work (pPD005, Addgene #138749)26 and further modified here to change the antibiotic resistance marker from ampicillin to kanamycin and to add the additional expression cassette containing a CMV promoter ...
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bioRxiv - Neuroscience 2023Quote: ... all plasmids were ordered and modified (if necessary) from Addgene (Watertown, MA). Control cells were transfected with pAdtrack-CMV (#16405) ...
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bioRxiv - Biophysics 2024Quote: ... The transfer vector consisted of a modified pLKO.1 neo plasmid (Addgene) with expression of the shRNA sequences under control of 3× copies of the lac operator ...