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Citations for Addgene :
251 - 300 of 1197 citations for PAS Domain Containing Serine threonine Protein Kinase PASK Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... We used rAAV-Ef1a-DIO-hChR2(H134R)-EYFP-WPRE-pA (Received as a gift from Karl Deisseroth, Addgene plasmid # 20298; http://n2t.net/addgene:20298; RRID:Addgene_20298) for Cre-mediated opsin expression ...
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bioRxiv - Neuroscience 2019Quote: ... We used rAAV-Ef1a-DIO-hChR2(H134R)-EYFP-WPRE-pA (Received as a gift from Karl Deisseroth, Addgene plasmid # 20298 ...
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bioRxiv - Neuroscience 2021Quote: ... of rAAV-RAM (rAAV-RAM-d2TTA::TRE-MCS-WPRE-pA) that was a kind gift from Yingxi Lin (Addgene plasmid # 63931; http://n2t.net/addgene:6393; RRID:Addgene_63931)) (Gulmez Karaca et al. ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The DMRT regulatory region was inserted into the plasmid pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6m-WPRE-pA (Addgene) cut with AvrII and BglII restriction enzymes.
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bioRxiv - Bioengineering 2023Quote: Full length plasmid of PI3KCA (phosphatidylinositol-4,5-biphosphate 3-kinase catalytic subunit alpha, NM_006218.4) was purchased from Addgene (Plasmid ID: 81736, Hahn and Root Lab). The coding sequence of PI3KCA was cloned into Lenti-PCDH-EF1-mNeonGreen-MCS-T2A-puromycin plasmid (a kind gift from Fırat-Karalar Lab ...
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bioRxiv - Neuroscience 2023Quote: ... LR reaction was performed using R83B04 containing entry vector and lexA containing (pBPLexA::p65Uw) destination vector (Addgene: 26231). Transgenic fly generation was conducted by GenetiVision ...
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bioRxiv - Cell Biology 2022Quote: A His-tagged mouse Capping Protein (F-actin-capping protein subunit alpha-1 and F-actin-capping protein subunit beta) plasmid was obtained from Addgene as described above (Plasmid #89950) ...
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bioRxiv - Microbiology 2020Quote: ... A plasmid containing the ACE2 gene (Addgene) was digested with Nhe I and Kpn I and the fragment was ligated into the similarly digested pLenti-III-HA vector plasmid ...
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bioRxiv - Cell Biology 2021Quote: Plasmids containing sirtuins were ordered from Addgene, and all of them were produced by Eric Verdin ...
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bioRxiv - Molecular Biology 2021Quote: ... a vector containing 24xMS2v6 (Addgene Plasmid #104391) was double-digested and the MS2 cassette was gel purified ...
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bioRxiv - Immunology 2021Quote: ... along with the DHFR containing vector (Addgene) to produce 2H1-mIgG3-CH1a-1 ...
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bioRxiv - Genomics 2021Quote: ... gene fragment containing CTT pegRNA (Addgene #132778) was PCR amplified using primer sets adding 5-bp degenerate barcode and flanking BsmBI site for the downstream cloning steps ...
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bioRxiv - Cancer Biology 2022Quote: Plasmids containing AcGFP and LbNOX (Addgene #75285) were gifts from Dr ...
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bioRxiv - Cancer Biology 2020Quote: ISG15-PA was synthesised in the Kessler lab using the ISG15 C-term (79-156) construct from Addgene (#110760) and DUB ABP assays were performed as previously described69 ...
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bioRxiv - Cell Biology 2021Quote: PuroR fragment amplified by PCR from DT-A-pA-loxP-PGK-Puro-pA-loxP (Ninagawa et al., 2014) was inserted into the PciI site of px330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene) to create px330-PuroR ...
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bioRxiv - Immunology 2023Quote: ... pBigT-mSparcl1 was then subsequently cloned into a plasmid with the ROSA26 genomic flanking arms (ROSA26-PA, Addgene, #21271) following the protocol described previously (51) ...
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bioRxiv - Neuroscience 2023Quote: ... The recombinant AAV to express SaCas9 was created using plasmid pAAV-EFS-SaCas9-P2A-HAFLAGHA-KASH-pA (Addgene, #113688). For constructs for REST4 expression ...
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bioRxiv - Bioengineering 2023Quote: ... the dCas9 activator modRNA was replaced with pPB-R1R2-EF1a-VP64dCas9VP64-T2A-MS2p65HSF1-IRES-bsd-pA plasmid (Addgene, #113341). Before transfection ...
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bioRxiv - Neuroscience 2023Quote: ... A mix of AAV1-Flex-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA and AAV9-CaMKII-0.4.Cre-SV40 (Addgene, 68720-AAV1 and 105558-AAV9 respectively ...
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bioRxiv - Neuroscience 2024Quote: ... was subcloned into the pAAV-RAM-d2TTA::TRE-MCS-WPRE-pA construct (kindly deposited to Addgene by Dr. Yingxi Lin; Addgene plasmid # 63931; http://n2t.net/addgene:63931; RRID: Addgene_63931). Overexpression of Dnmt3a2 or a Control was achieved by using published and validated constructs34 ...
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bioRxiv - Plant Biology 2019Quote: ... the resulting gRNA plasmids were then recombined with a L1 construct containing pYAO:Cas9_3:E9t39 (kindly provided by Jonathan Jones) and a L1 construct containing Fast-Red selection marker (AddGene #117499) into a L2 binary vector (AddGene #112207).
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bioRxiv - Developmental Biology 2022Quote: ... Samples were incubated with Protein A/G-MNase fusion protein (Addgene, Cat #123461) at 700 ng/mL for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... (The pET21b plasmid was a kind gift from the Tonks Group of Cold Spring Harbor Laboratory; we purchased pTriEx-PA-Rac1 from Addgene). We joined the two amplified segments with overlap extension PCR (oePCR ...
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bioRxiv - Bioengineering 2021Quote: AAV transduction of the genetically encoded GCaMP6s calcium reporter and mRuby control tag (AAV1-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA, AddGene) was performed at DIV1 as previously described50 ...
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bioRxiv - Neuroscience 2021Quote: ... The GCaMP6s reporter was expressed in neurons under the human synapsin promoter following successful AAV transduction (AAV1-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA, AddGene). Briefly ...
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bioRxiv - Cell Biology 2021Quote: Transgene Ppif K166Q construct was cloned into the Double-floxed Inverted Orientation (DIO) Cre-On vector pAAV-Ef1a-DIO-eGFP-WPRE-pA (Addgene plasmid # 37084 ...
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bioRxiv - Neuroscience 2020Quote: ... AAV-DIO-hChR2-mCherry was generated using pAAV-Ef1a-DO-hChR2(H134R)-mCherry-WPRE-pA plasmid as backbone (ADDGENE #37082). Vectors used were of an AAV1/AAV2 mixed serotype ...
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bioRxiv - Neuroscience 2020Quote: ... Both fragments were ligated to produce the final construct pAAV-ITR-PTRE-tight-flex-ChR2-EYFP-WPRE-pA-ITR (to be deposited at Addgene).
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bioRxiv - Neuroscience 2021Quote: ... The RAM-HA-GFP viral construct was generated by insertion of a HA-tagged GFP expression cassette into the multiple cloning site (MCS) of rAAV-RAM (rAAV-RAM-d2TTA::TRE-MCS-WPRE-pA) that was a kind gift from Yingxi Lin (Addgene plasmid # 63931 ...
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bioRxiv - Microbiology 2021Quote: ... and pCT-CD63-GFP (SBI, CYTO120-PA-1) plasmids were transfected in HEK293T cells with the packaging plasmids pMD2.G (Addgene; number 12259 ...
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bioRxiv - Neuroscience 2023Quote: ... adeno-associated virus (AAV) construct that allows Cre-mediated expression of the transgene (pAAV-EF1A-DIO-WPRE-pA vector; RRID Addgene_39320) (Belmer et al. ...
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bioRxiv - Cell Biology 2022Quote: ... Both plasmids contain the left and right homology arm to the AAVS1 genomic integration site derived from the AAVS1 SA-2A-puro-pA donor plasmid (Addgene) (Kotin et al. ...
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bioRxiv - Physiology 2023Quote: ... the CAG-pai expression cassette was inserted into the Pac I and Asc I sites of the pROSA26-PA backbone vector (Gift from Dr Frank Costantini, Addgene plasmid#21271 ...
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bioRxiv - Neuroscience 2023Quote: ... of adeno-associated viruses encoding the genetically-encoded calcium indicator GCaMP6s and mRuby2 as a structural marker under the control of the synapsin 1 promoter (pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA, cat. no. 50942-AAV1, Addgene) and slowly lowered to ∼350 µm below the surface of the exposed brain with an HO-10 hydraulic micromanipulator (Narishige) ...
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bioRxiv - Neuroscience 2023Quote: ... and AAV2/9-EF1a-SaCas9-P2A-HA produced from the plasmid pAAV-EFS-SaCas9-P2A-HAFLAGHA-KASH-pA (Addgene #113688) (46) ...
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bioRxiv - Neuroscience 2024Quote: ... was subcloned into the pAAV-RAM-d2TTA::TRE-MCS-WPRE-pA construct (kindly deposited to Addgene by Dr. Yingxi Lin; Addgene plasmid # 63931 ...
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bioRxiv - Biophysics 2020Quote: ... Dual transfections containing mCh-Drp1 (Addgene, plasmid #49152) and Mito-GFP (gift from Hari Shroff ...
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bioRxiv - Systems Biology 2021Quote: ... Sleeping Beauty transposase containing pSB100 vector (Addgene #34879) was a kindly gift of Dr ...
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bioRxiv - Neuroscience 2020Quote: ... The AAV vector containing floxed ChR2 (Addgene #20297) and floxed eNpHR (Addgene #26966 ...
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bioRxiv - Genetics 2022Quote: ... Jurkat cells containing pLenti-PE2-BSD (Addgene, #161514) were treated with 10 μg/ml blasticidin for selection of blasticidin and incubated for 72 hours ...
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bioRxiv - Cell Biology 2021Quote: FRET biosensor containing vectors: pTriEx4-Rac1-2G (Addgene plasmid # 66110 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: pBABE retroviral vector containing GFP-LC3 (Addgene, 22405) was transfected into PT67 cells (ATCC ...
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bioRxiv - Neuroscience 2022Quote: ... into a pAAV backbone containing stChrimsonR-mRuby2 (RRID:Addgene_105447) and packaged into an AAV (Vigene ...
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bioRxiv - Molecular Biology 2022Quote: ... Jurkat cells containing pLenti-PE2-BSD (Addgene, #161514) were treated with 10 µg/ml blasticidin for selection of blasticidin and incubated for 72 hours ...
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bioRxiv - Neuroscience 2023Quote: Plasmids containing pAAV-hsyn-ChrimsonR-tdTomato (Addgene # 59171) (51 ...
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bioRxiv - Immunology 2023Quote: A lentiviral construct containing human ACE2 (Addgene 155295) or mScarlet (Addgene 85044 ...
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bioRxiv - Physiology 2023Quote: ... containing human Fis1 gene were purchased from Addgene. The working viral vectors were constructed from these two plasmids by Custom DNA Constructs ...
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bioRxiv - Molecular Biology 2023Quote: ... MED1-IDR containing plasmid was obtained from Addgene #145276 and inserted into pcDNATM6.2-C-EmGFP-DEST supplemented with the Simian Virus 40 NLS sequence by Gateway strategy (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... Separate lentivectors containing EGFP-NPM1 (Addgene plasmid # 17578) and mRuby2-Lamin A/C (Addgene plasmid # 55901 ...
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bioRxiv - Immunology 2021Quote: ... green fluorescence protein (GFP) or an enhanced yellow fluorescent protein (EYFP) sequence (pcDNA1.3-, Addgene) using standard cloning methods ...