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Citations for Addgene :
101 - 150 of 1197 citations for PAS Domain Containing Serine threonine Protein Kinase PASK Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... as well as the CD8α hinge and transmembrane domains were cloned into pRRLSIN.cPPT.PGK-GFP.WPRE (Addgene # 12252) or SFG.CNb30_opt.IRES.eGFP (Addgene # 22493 ...
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bioRxiv - Synthetic Biology 2022Quote: ... The Spy and Snoop catcher domains were cloned from the pET28a-SpyCatcher-SnoopCatcher plasmid (Addgene, #72324) while the Traptavidin domain came from pET21a-Core-Traptavidin (Addgene ...
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bioRxiv - Microbiology 2023Quote: pT7-S1/HER2nhd (no head domain) was constructed using pBacT7S1-T3D (Addgene, (Kobayashi et al., 2007) as a backbone plasmid ...
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bioRxiv - Cell Biology 2023Quote: ... The full-length and domain of interest were amplified by PCR using emGFP-BAZ1B (#65372; Addgene) as a template and inserted into pEGFP-C2 vector (Takara Bio ...
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bioRxiv - Cell Biology 2024Quote: ... NTF2 domain of G3BP1 variants was cloned into a pET28 MBP-TEV bacterial backbone (Addgene, 69929) with Gibson assembly.
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bioRxiv - Cell Biology 2022Quote: Rac1 activation was achieved by expressing PA-Rac1 via retro-virus infection using pBabe-TetCMV-puro-mCherry-PA-Rac1 (Addgene Plasmid #22035) in MV3 cells ...
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bioRxiv - Neuroscience 2020Quote: The 3xFLAG pA-fusion sequence was copied from Addgene plasmid #124601 and inserted into psfTn5 plasmid (addgene #79107 ...
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bioRxiv - Neuroscience 2024Quote: ... QF2 was amplified from Gbait-hsp70-QF2-pA (Addgene plasmid #122563 ...
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bioRxiv - Developmental Biology 2022Quote: ... a solution containing two sgRNAs (40 ng/μL each) and Cas9 protein (250 ng/μL) (Addgene; 47327) (Gagnon et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... fragments containing promoter and fusion protein segments were digested and cloned into the pKHR4 vector (Addgene #74592) using SpeI (NEB #R3133 ...
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bioRxiv - Microbiology 2020Quote: ... Plasmid expressing the catalytic domain of PKCδ was previously described (Soh and Weinstein, 2003) (Addgene plasmid #16388).
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bioRxiv - Developmental Biology 2021Quote: ... a FoxO1 plasmid encoding a deleted DNA binding domain (amino acid 208-220) was used (Addgene #10694). After 24 hours ...
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bioRxiv - Microbiology 2023Quote: ... reverse primer: 5’ – GAGTCGggatccACTAGTAGTTCCTGCTATGTCACTTCCCCTTGG – 3’) and ligating the domain into the pUC19 cloning vector (Addgene plasmid # 50005), using the T4 ligase ligation kit (New England Biolabs ...
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bioRxiv - Cancer Biology 2023Quote: ... EF.deltaBHES1.Ubc.GFP (DBD-HES1 DNA-binding domain mutant of HES1; #24982) were obtained from Addgene (Cambridge, USA). The reporter plasmids containing the firefly luciferase gene under the control of various fragments of the Hes1 promoter (2.5 kb ...
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bioRxiv - Biophysics 2023Quote: A plasmid encoding the GST-tagged human afadin PDZ domain was a gift from Sachdev Sidhu (Addgene plasmid # 103938 ...
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bioRxiv - Cell Biology 2024Quote: ... For G3BP1 live imaging a GFP-G3BP1 fusion protein containing plasmid (pEGFP-C1-G3BP1-WT, Addgene plasmid # 135997) was transfected with Lipofectamine™ 3000 Transfection Reagent (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... using AAVS1 SA-2A-puro-pA donor plasmid (#22075, Addgene) as a template ...
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bioRxiv - Neuroscience 2023Quote: ... AAVDJ-EF1a-DIO-hChR2(H134R)-EYFP-WPRE-pA (# 20298, Addgene), AAV1-DIO-saCas9-sgSlc17a6 (The Zweifel Lab) ...
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bioRxiv - Synthetic Biology 2023Quote: - “Px-PhiYFP-triplex-28-M13-28-pA” (Addgene ID 202047) - contains a cloning site in place of the promoter so that targeting arrays can be introduced upstream of the non- coding transcript harboring a 28 nt-flanked gRNA cloning site in the 3’ UTR ...
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bioRxiv - Synthetic Biology 2023Quote: - “Px-PhiYFP-triplex-DR-M13-DR-pA” (Addgene ID 202046) - contains a cloning site in place of the promoter so that targeting arrays can be introduced upstream of the non- coding transcript harboring a DR-flanked crRNA cloning site in the 3’ UTR ...
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bioRxiv - Neuroscience 2023Quote: ... and pAAV-RAM-d2TTA::TRE-eGFP-WPRE-pA (Addgene# 84469) were a kind gift from Yingxi Lin ...
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bioRxiv - Neuroscience 2024Quote: ... AAV2-retro virus (rAAV1-retro-EF1a-DO_DIO_TdTomato_EGFP-WPRE-pA, Addgene #37120 ...
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bioRxiv - Genetics 2021Quote: ... and fused in frame with the human ZNF10 KRAB domain (amplified from the pAAVS1-NDi-CRISPRi (Addgene #73498)) or the catalytic domain (CD ...
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bioRxiv - Genomics 2022Quote: ... dCas9-KRAB domain (pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro was a gift from Charles Gersbach (Addgene plasmid # 71236 ...
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bioRxiv - Cell Biology 2022Quote: ... The RhoA biosensor uses the AnillinAHPH domain (Piekny and Glotzer, 2008) and was obtained from Addgene (plasmid # 68026). The EGFP-AnilinAHPH was sub-cloned into pHR lentiviral backbone using similar sites and strategy as mentioned above ...
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bioRxiv - Cancer Biology 2022Quote: ... Control and SUZ12 sgRNA targeting the VEFS functional domain were cloned into pL-CRISPR.EFS.Blast (plasmid was generated by replacing tagRFP in Addgene Plasmid #57819 with blasticidin S deaminase antibiotic selection marker ...
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bioRxiv - Genetics 2022Quote: ... We made the dCas9-TurboID construct by replacing the KRBA domain on Lenti-dCas9-KRAB-blast (Addgene, 89567) with TurboID sequence (Addgene ...
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bioRxiv - Synthetic Biology 2022Quote: ... This receptor harbors a myc-tag on its extracellular domain that can be visualized by immunostaining (Addgene #79128). A second virion expresses both the mCherry reporter and BFP under control of the Tetracycline responsive element (TRE-3G ...
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bioRxiv - Molecular Biology 2023Quote: ... co-transfected with the domain III/pURD construct and an integrase expression vector pgk-φC31/pCB92 (Addgene #18935) [50] ...
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bioRxiv - Biophysics 2023Quote: A plasmid encoding the GST-tagged human afadin PDZ domain was a gift from Sachdev Sidhu (Addgene plasmid # 103938 ; http://n2t.net/addgene:103938; RRID:Addgene_103938). Plasmids encoding tethered fusion constructs were custom cloned by Epoch Biosciences (Missouri City ...
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bioRxiv - Cell Biology 2022Quote: Full-length ERK3 wild type (WT) pDONR-223 construct purified from Human Kinase Library (Addgene) was used as a template to generate ERK3 K49A K50A kinase dead (KD ...
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bioRxiv - Cell Biology 2023Quote: ... we expressed the ULK1 kinase from a pCAG backbone encoding MBP-TSF-TEV-ULK1 (RRID:Addgene_171416). The transfection and expression procedure was similar to what is described above for NAP1 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... a plasmid containing cloned wild type AlkB protein (pET24a-AlkB deltaN11 [plasmid #73622]) was obtained from Addgene (http://www.addgene.org/), and the AlkB protein was expressed and purified at the CSU Biochemistry and Molecular Biology Protein Expression and Purification Facility.
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bioRxiv - Biochemistry 2020Quote: ... and substrate domain (i.e., WMEDYDYVHLQG, a peptide derived from p130cas)—from its parent plasmid (a Kras-Src FRET biosensor, Addgene), (ii ...
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bioRxiv - Genomics 2021Quote: Individual effectors were fused at the N-terminus to the PYL1 domain (gift from Jerry Crabtree, Addgene plasmid #38247) and sfGFP ...
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bioRxiv - Synthetic Biology 2022Quote: ... the scFV domain and a superfolder (sf)GFP coding sequence were amplified from the PlatTET-gRNA2 plasmid (Addgene #82559) and fused in frame with the catalytic domain (CD ...
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bioRxiv - Plant Biology 2022Quote: ... and HlWRKY1 was individually cloned into the bait vector pGBKT7-GW [DNA-binding domain (BD)] (Addgene Inc, MA, USA) to produce fusion proteins containing a GAL4 activation domain (AD ...
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bioRxiv - Neuroscience 2021Quote: ... AAVrg-EF1a-DO_DIO-tdTomato_EGFP (pAAV-Ef1a-DO_DIO-TdTomato_EGFP-WPRE-pA, Addgene) for anatomical tracing of STN projections to MLR ...
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bioRxiv - Cell Biology 2022Quote: ... Sobol (University of Pittsburgh School of Medicine, Pittsburgh, PA; Addgene; 128660) for double-labeled stable cell line creation.
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bioRxiv - Synthetic Biology 2024Quote: 8xCTS sequences were cloned in the P2-ECFP-pA (Addgene #26280) plasmid generated by Nissim et al (Nissim et al ...
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bioRxiv - Cell Biology 2019Quote: ... pET-3d plasmid containing Chicken Capping Protein α1 and β1 subunits was a gift from John Cooper (Addgene plasmid #13451) The N-terminal domain of mouse CAP1 in pSUMOck4 was described earlier66.
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bioRxiv - Evolutionary Biology 2020Quote: ... five nanoliters of a solution containing both sgRNAs at a concentration of 40 ng/μL and Cas9 protein (Addgene #47327) at a concentration of 250 ng/μL32 was injected into one-cell-stage medaka embryos ...
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bioRxiv - Biochemistry 2022Quote: The pET28a plasmid vector containing DNA sequences encoding the PANK3 protein (residues pro12 to Asn368) was purchased from Addgene (25518) and transformed into both E ...
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bioRxiv - Biochemistry 2022Quote: ... All expression constructs were in the form containing an N-terminal His6 and maltose-binding protein (MBP) tag (Addgene #11517).
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bioRxiv - Biochemistry 2023Quote: ... 2 µg of pX330 plasmid containing sgRNA for the target protein and 0.2 µg of pcDNA3-FKBP-eGFP-HOTag3 (Addgene, #106924) were co-transfected using Lipofectamine 2000 (Thermo Scientific ...
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bioRxiv - Genetics 2021Quote: ... For the effector plasmids (pPB_TRE3G::ScFv-GFP-KRAB_EF1a::Neo and pPB_TRE3G::ScFv-GFP-3a3L_EF1a::Neo) the GCN4 specific scFv domain and the sfGFP gene were amplified from PlatTET-gRNA2 plasmid (Addgene #82559) and fused in frame with the human ZNF10 KRAB domain (amplified from the pAAVS1-NDi-CRISPRi (Addgene #73498) ...
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bioRxiv - Cell Biology 2019Quote: The Polo-like binding domain (PBD) of PLK1 (amino acid 326 to amino acid 603) was amplified from the pTK24 plasmid (Addgene) and cloned into a pT7-His6-SUMO expression vector using NEB Gibson assembly (Gibson Assembly Master Mix ...
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bioRxiv - Biochemistry 2020Quote: Recombinant wild-type Streptococcus pyogenes Cas9 REC3 domain (506-712) possessing an amino-terminal His10-MBP tag (Addgene, no. 101205) followed by a TEV protease site was expressed in Escherichia coli strain BL21(DE3 ...
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bioRxiv - Neuroscience 2022Quote: ... Err2EnR was generated by fusing the open reading frame of the transcriptional repressor domain of Drosophila Engrailed (amplified from CAG-EnR plasmid, Addgene plasmid #19715 ...
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bioRxiv - Cell Biology 2022Quote: ... gBlock 1 encoding N-terminus Calponin domains (CH-CH) of giant Nesprin 1 was cloned into Spectrin-cpstFRET (plasmid#61109, Addgene) cut with AgeI and ScaI renstriction enzymes (New England Biosciences) ...