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Citations for Addgene :
51 - 100 of 1197 citations for PAS Domain Containing Serine threonine Protein Kinase PASK Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... or PA-mCit (Addgene #113443) were used as background controls ...
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bioRxiv - Molecular Biology 2022Quote: ... or pRK5-MYC-mTOR kinase-dead (Addgene plasmid #8482)) using the TransIT-X2 transfection reagent (Mirus ...
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bioRxiv - Neuroscience 2023Quote: ... and cpGFP and RSET domain were subcloned from GCaMP6f (Addgene plasmid # 52924). To enhance cytoplasmic localization of cAMPinG1 ...
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bioRxiv - Neuroscience 2023Quote: ... To introduce the domain-specific protospacer sequences into the pCFD4 vector (Addgene), we employed the ligation-independent Gibson Assembly method (NEBuilder ...
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bioRxiv - Microbiology 2024Quote: The KRAB domain from pHR-SFFV-dCas9-BFP-KRAB (Addgene plasmid #46911) was cloned into plasmid pHR-SFFV-ZF3-P2A-BFP using Gibson assembly (New England Biolabs Cat#E2611S ...
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bioRxiv - Developmental Biology 2023Quote: Plasmids #123461 (pA/G-MNase) and #124601 (3XFlag-pA-Tn5-Fl) were ordered from Addgene. ProteinA and ProteinG were amplified using the primer pairs (FZ461_ProtA_rev ...
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bioRxiv - Cell Biology 2023Quote: ... was synthesized and cloned into a vector containing homology arms targeting the AAVS1 safe harbor site (AAVS1 SA-2A-puro-pA donor vector (Addgene: Plasmid #22075)(60)) ...
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bioRxiv - Biophysics 2020Quote: ... MEFs we infected with retrovirus encoding PA-Rac1 (pBabe-TetCMV-puro-mCherry-PA-Rac1, Addgene #22035) and selected for 1 week with 10 ng/mL of puromycin (10ng/ml) ...
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bioRxiv - Cancer Biology 2022Quote: ... and constitutively kinase-active (#64608) IKKα were purchased from Addgene. Briefly ...
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bioRxiv - Systems Biology 2021Quote: ... additional control plasmids (PA-NL, Addgene #113445 ...
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bioRxiv - Synthetic Biology 2023Quote: - “Px-mCerulean-pA” (Addgene ID 202048) - contains a cloning site in place of the promoter so that targeting arrays can be introduced upstream of the mCerulean reporter gene ...
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bioRxiv - Systems Biology 2023Quote: ... additional control plasmids (PA-NL, Addgene #113445 ...
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bioRxiv - Neuroscience 2023Quote: PA sfGFP-N1 (Plasmid #54736 Addgene) was used as the backbone to separate it the same way as mentioned above ...
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bioRxiv - Synthetic Biology 2023Quote: - “Px-PhiYFP-pA” (Addgene ID 202045) - contains a cloning site in place of the promoter so that targeting arrays can be introduced upstream of the PhiYFP reporter gene ...
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bioRxiv - Synthetic Biology 2023Quote: - “Px-mCherry-pA” (Addgene ID 202049) - contains a cloning site in place of the promoter so that targeting arrays can be introduced upstream of the mCherry reporter gene ...
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bioRxiv - Neuroscience 2019Quote: ... pyogenes dCas9 fused to two copies of the VP64 transactivator domain (Addgene #59791). Endotoxin-free plasmids were purified using an endotoxin-free plasmid DNA purification kit (Machery-Nagel ...
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bioRxiv - Genomics 2021Quote: ... KRAB and MeCP2 were linked to dCas9 with flexible glycine-serine linkers and cloned into lentiCas9-Blast (Addgene 52962) (22).
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bioRxiv - Microbiology 2023Quote: ... plasmid containing human codon-optimized Streptococcus pyogenes Cas9 protein and Stk3 (Mst2, Addgene #75975) gRNA was used to dually target Mst1 and Mst2 ...
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bioRxiv - Biochemistry 2019Quote: ... and pFLAG-SREBP2Nt (N-terminal transcriptionally active domain, amino acids 1-482) (Addgene, 26807) were used to transfect cells (HEK293) ...
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bioRxiv - Synthetic Biology 2019Quote: ... The LOV2 domain was PCR amplified from the vector CMV-CASANOVA (Addgene plasmid #113035) previously reported by us (29) ...
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bioRxiv - Cancer Biology 2019Quote: ... the KRAB domain was PCR amplified using pHR-SFFV-dCas9-BFP-KRAB (Addgene #46911) as a template ...
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bioRxiv - Genomics 2021Quote: ... Clover fused with PUF RNA-binding domain were previously described: pAC1447 (Clover_PUFc) (Addgene #73689). PUF9R-tethered iRFP670 and mRuby2 were created by a combination of PCR (from IDT gBlocks ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmids encoding HA-tagged LRR (leucine-rich-repeat) domains of LRRFIP2 (Addgene plasmid # 21152), and Fli1 (Addgene plasmid # 21151) ...
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bioRxiv - Genetics 2022Quote: ... The ecDHFR degron domain was amplified from CAG-DDdCas9VP192-T2A-EGFP-ires-puro (Addgene plasmid # 69534 ...
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bioRxiv - Genomics 2024Quote: Clover fused with PUF RNA-binding domain were previously described: pAC1447 (Clover_PUFc) (Addgene #73689). Cloning of dCas9 expression plasmid (lenti-dCas9-Blast ...
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bioRxiv - Cell Biology 2022Quote: PA-mCherry1-C1 plasmid was from Addgene (gift from Dr ...
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bioRxiv - Neuroscience 2023Quote: ... and AAV5-hSyn-EGFP-pA (#50465, Addgene).
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bioRxiv - Systems Biology 2023Quote: ... pcDNA3.1-PA-mCit (Addgene plasmid ID #113443) were kindly provided by the Wanker Group (Max-Delbrück-Centrum für Molekulare Medizin ...
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bioRxiv - Neuroscience 2023Quote: ... and AAV2retro-cFos-tTA-pA (Addgene #66794). The AAV2retro-CamKIIa-jGCaMP8s-WRPE virus (Addgene #176752 ...
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bioRxiv - Neuroscience 2024Quote: ... Gbait-hsp70-QF2-pA (Addgene plasmid #122563), which contains a GFP bait sequence for Cas9-mediated linearization of the donor plasmid (Kimura et al. ...
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bioRxiv - Neuroscience 2024Quote: ... p3E-mKate2-myc no-pA (Addgene, 80812), and pDESTtol2pACrymCherry (Addgene ...
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bioRxiv - Developmental Biology 2024Quote: ... The pA/G-MNase (Addgene ID: 123461) used for the CUT&RUN experiments was purified at UNC-Chapel Hill’s Protein Expression and Purification core.
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bioRxiv - Molecular Biology 2021Quote: ... The expression vectors of the full-length SARS-CoV-2 spike and the human serine protease TMPRSS2 with a C-terminal C9-tag (TETSQVAPA) were acquired from AddGene (Summit Pharmaceutical International ...
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bioRxiv - Microbiology 2022Quote: ... to co-transfect plasmids containing cDNAs for SARS-CoV-2 E protein (pGBW-m4133502, Addgene) and green fluorescent protein (GFP ...
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bioRxiv - Developmental Biology 2020Quote: The histone acetyltransferase domain from human p300 was subcloned from a published construct (Addgene, 61357) (Hilton et al. ...
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bioRxiv - Cancer Biology 2019Quote: The mSMO and ShhN (Shh N-terminal domain) plasmids purchased from Addgene (#37673 and #37680). All vectors were propagated in XL10 Gold competent cells ...
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bioRxiv - Neuroscience 2021Quote: ... mCherry-SH2-mCherry was prepared from the C-terminal SH2 domain of p85a (Addgene #46463). The linker between mCherry and SH2 is SGLRSRAQASNSAVDGTA for the N terminus and GSG for the C terminus.
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bioRxiv - Cell Biology 2022Quote: ... were transformed with a plasmid encoding the GST-tagged domain of Rhotekin (Addgene plasmid # 15247). Cells were cultivated in Terrific broth (TB ...
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bioRxiv - Bioengineering 2020Quote: ... KRAB domain was derived from pHAGE EF1α dCas9-KRAB (Addgene 50919, Kearns et al., 2014). VPR complex was derived from lenti-EF1a-dCas9-VPR-Puro (Addgene 99373 ...
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bioRxiv - Neuroscience 2019Quote: ... was constructed by amplifying the KASH domain from (Addgene 60231, a gift of Feng Zhang) and fusing it (in-frame ...
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bioRxiv - Microbiology 2023Quote: ... All PNP domains tested for toxicity were cloned into the pBbA6c vector65 (Addgene plasmid # 35290) under the IPTG-inducible pLac promoter.
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bioRxiv - Genetics 2023Quote: ... and the NLS-Gal4AD domain from a plasmid originally based on pActL-Gal4AD (Addgene 15303) and the 127D01 tag was inserted as part of the primer ...
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bioRxiv - Cancer Biology 2023Quote: ... and CD28TM CAR domains were amplified from the plasmids pSLCAR-CD19-hCAR-BB (Addgene, #135992) and pSLCAR-CD19-28z (Addgene ...
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bioRxiv - Cell Biology 2020Quote: ... A negative selection cassette with human thymidine kinase was retrieved from Addgene #21911 (41) ...
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bioRxiv - Cell Biology 2019Quote: ... and pTriEx-PA-Rac1-I539E (Addgene plasmid # 22026) were kindly provided by Klaus Hahn (Wang et al. ...
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bioRxiv - Biophysics 2020Quote: ... and SB100X in pCAG globin pA (Addgene 127909). 48 hours post-transfection ...
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bioRxiv - Neuroscience 2020Quote: ... plasmid vector pCAG-T3-hCAS-pA (Addgene 48625) was linearized by Sph I ...
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bioRxiv - Cancer Biology 2024Quote: ... containing a C-terminus EGFP-tagged sequence of the full-length M237I p53 protein (Addgene, #11770), and 4 µL of Lipofectamine 2000 reagent ...
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bioRxiv - Cell Biology 2020Quote: ... pGEX6P1-human LIC1 G domain (GST-LIC 1-389) was a gift from Ron Vale (Addgene plasmid # 74598 ...
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bioRxiv - Immunology 2021Quote: ... C domain coding sequences of human CRT were cloned into the pCMV vector (Addgene plasmid #59314) to generate recombinant constructs with C-terminal Human IgG1Fc (Ig ...