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Citations for Addgene :
301 - 350 of 1197 citations for PAS Domain Containing Serine threonine Protein Kinase PASK Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... mCherry fluorescent protein marker (Addgene), and ampicillin resistance gene ...
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bioRxiv - Immunology 2019Quote: ... were incorporated into two complementary 100-mer oligonucleotides and cloned into a gRNA containing plasmid containing the (NeoR/KanR) cassette (Addgene # 41824). The human codon optimized pCAGGS-Cas9-mCherry was used for gene-editing experiments (a gift from Stem Cell Core Facility at Columbia University) ...
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bioRxiv - Molecular Biology 2023Quote: ... An unmethylated plasmid containing the rDNA promoter sequence and/or an unmethylated plasmid containing the HpaII cut site (pUC19, Addgene# #50005) were digested alongside the experiments to ensure efficient digestion ...
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bioRxiv - Developmental Biology 2021Quote: The mouse Trim41 cDNA (ENSMUST00000047145) tagged with PA and 1D4 was inserted under the control of the mouse Clgn promoter (Addgene #173686) (Ikawa et al. ...
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bioRxiv - Genetics 2020Quote: ... The same kit was used to produce Cas9 D10A mRNA using as template the plasmid pCAG-T3-hCasD10A-pA (Addgene #51638). The 140bp ssDNA homology direct repair (HDR ...
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bioRxiv - Biophysics 2020Quote: ... A homozygous clone that passed all QC (U2OS HP1⍺ 4) was co-transfected with the transposon vector pEF1a-OsTIR-IRES-NEO-pA-T2BH (Addgene 127910) and SB100X in pCAG globin pA (Addgene 127909) ...
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bioRxiv - Cell Biology 2020Quote: DNA templates used for in vitro transcription of mouse Xist RNA were amplified from sequence verified plasmid pCMV-Xist-PA (Wutz and Jaenisch, 2000) (Addgene 26760), containing the murine Xist gene using high-fidelity Platinum® pfx DNA polymerase (Invitrogen™) ...
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bioRxiv - Neuroscience 2022Quote: ... We injected a viral cocktail of AAV5-CamKIIa-C1V1(E122T/E162T)-TS-eYFP-WPRE-hGH (2.5 × 1012 virus molecules/ml; Penn Vector Core, University of Pennsylvania, PA, USA, Addgene number: 35499) in the primary sensory (S1 ...
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bioRxiv - Genetics 2019Quote: ... Plasmid phU6-gRNA-CBh-hCas9 was obtained from Rupesh Amin and Mark Groudine and first modified by insertion of the PGK-puro-pA selectable marker from pPGKpuro (Addgene #11349) 74 to obtain pPuro2-hU6-gRNA-CBh-Cas9 ...
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bioRxiv - Cell Biology 2022Quote: ... and mitoPLD-GFP overexpression constructs were kind gifts from Hiromi Sesaki (Johns Hopkins) and described previously.51 The PA-PLA1-GFP overexpression construct was from Addgene (#162880). PerkWT and PerkPSP overexpression plasmids were kind gifts from Jonathan Lin (Stanford) ...
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bioRxiv - Neuroscience 2022Quote: ... In earlier experiments AAV1:CBA:FLEX:Arch-eGFP (200nl;5.48x1012 vg/ml; AV-1- PV2432; University of Pennsylvania vector core, Philadelphia, PA, USA, now at Addgene, 22222 - AAV1) was used ...
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bioRxiv - Bioengineering 2022Quote: ... and the dsRed filler fragment was generated by amplifying a portion of the dsRed gene from CMVp-dsRed2-Triplex-HHRibo-gRNA1-HDVRibo-pA (Addgene 55201) with primer set AA-sg1-scaf-short-filler-FW/AA-filler-SpeI-gib-RV in separate PCR reactions ...
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bioRxiv - Neuroscience 2023Quote: ... pAAV-cFos-tTA-pA and pAAV-PTRE-tight-hM3Dq-mCherry were a gift from William Wisden (Addgene plasmid #66794 and #66795). AAV vectors were serotyped with AAV9 coat proteins and packaged at the Viral Production Unit (UPV ...
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bioRxiv - Neuroscience 2024Quote: ... 200 μL of the viral cocktail AAV5-CamKIIa-C1V1(E122T/E162T)-TS-eYFP-WPRE-hGH (2.5 × 1012 virus molecules/mL; Penn Vector Core, University of Pennsylvania, PA, USA, Addgene number: 35499) was administered across four sites into the primary somatosensory (S1 ...
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bioRxiv - Molecular Biology 2020Quote: ... The BirA Ligase containing plasmid was purchased (Addgene #64395). The BirA Ligase was cloned in frame to the C/EBPα C-terminus using BamHI and XhoI restriction sites ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli S2060 cells were prepared containing pJC175e (Addgene #79219), a plasmid expressing pIII under control of the phage shock promoter32 ...
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bioRxiv - Microbiology 2021Quote: ... The recombinant plasmid containing human ACE2 gene (Addgene #1786) was transfected into HEK293T cells using Lipofectamine 2000 Transfection Reagent (Thermo Fisher ...
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bioRxiv - Cancer Biology 2022Quote: ... plasmid containing full-length E6 and its pLentiN (RRID:Addgene_37444) vector backbone control were used to stably express E6 ...
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bioRxiv - Molecular Biology 2021Quote: ... The plasmid containing BDNF cDNA (pCMV6 BDNF, Addgene #39857) was a gift from Dr ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmid DNA containing desmosome genes were purchased from Addgene, [Dsc2a no ...
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bioRxiv - Plant Biology 2023Quote: ... backbones together with parts containing LucN (pEPYC0CM0133, Addgene #154595), a C-terminal 3×FLAG® epitope tag (pICSL50007 ...
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bioRxiv - Systems Biology 2023Quote: ... with 1000 ng plasmid containing inducible HGF (Addgene #188749) or a constitutive pEF1α-HGF (Addgene #188748 ...
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bioRxiv - Cell Biology 2023Quote: ... A fragment containing 24xGCN4_v4 repeats was derived from Addgene plasmid #74928 ...
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bioRxiv - Neuroscience 2023Quote: Vectors containing the identified fluorophores obtained from Addgene (Addgene.com) were cloned into plasmids under the CMV promoter ...
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bioRxiv - Cell Biology 2023Quote: ... wash buffer containing 500 nM SUMOEu1 protease (Addgene #149333) was added to the beads and left to incubate for 30 minutes with mixing at 4°C ...
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bioRxiv - Synthetic Biology 2024Quote: A plasmid containing GCaMP6m was obtained from Addgene (36) and gblocks were obtained from IDT ...
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bioRxiv - Bioengineering 2023Quote: ... Plasmid containing LSSmApple is available from AddGene (Plasmid #191259). LSSmApple spectral data have been uploaded to FPbase and are accessible at https://www.fpbase.org/protein/lssmapple/.
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bioRxiv - Neuroscience 2022Quote: ... expressing mRuby2 and GCaMP6s under the human synapsin promoter (pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA) was purchased from Addgene (50942-AAV1). NPCs were differentiated to neural cells as described above and transduced by adding 1.44 μl virus solution per well in a μ-Slide 4 Well 5 days after cell seeding ...
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bioRxiv - Neuroscience 2022Quote: ... pAAV-EF1α-DIO-GCaMP6m-WPRE-pA or pAAV-GfaABC1D-EGFP-WPRE-pA) with pAAV-RC2/11 (or pAAV-RC2-retro or pAAV-RC9) and pAdDeltaF6 (Addgene plasmid #112867) were co-transfected into HEK-293T cells at the molecular ratio of 1:1:1 ...
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bioRxiv - Neuroscience 2022Quote: Pressure injections of AAV9 hSyn.jRGECO1a (totalling 0.5 × 1010 genomic copies in a volume not exceeding 200 nL, initially supplied by Penn Vector Core, PA, USA; and later by Addgene, MA, USA) and AAV2/5.GFAP.iGluSnFR.A184S (0.1 × 1010 genomic copies ...
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bioRxiv - Synthetic Biology 2023Quote: ... we designed a construct for the first tier of the dCas9-based cascade that is expressed from the EF1α promoter and contains a SapI-flanked cloning site in the 3’UTR for adding a gRNA to target a downstream target: “EF1a-Triplex-28-M13-28-pA” (Addgene ID 202041). Detailed protocols for modifying all of these constructs are provided on the Addgene website.
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Hippocampal efferents to retrosplenial cortex and lateral septum are required for memory acquisitionbioRxiv - Neuroscience 2020Quote: ... enhanced yellow fluorescent protein (eYFP) or enhanced green fluorescent protein (eGFP): AAV1.CaMKIIa.eNpHR3.0.eYFP.WPRE.hGH (Addgene, #26971), AAV1.CaMKII.eYFP ...
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bioRxiv - Genomics 2019Quote: ... we cloned effector proteins (PguCas13b: Addgene 103861 ...
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bioRxiv - Genetics 2019Quote: ... Cas9 protein and mRNA (44758; Addgene) were co-microinjected into zygotes [F1 hybrids between strains FVB/NJ and B6(Cg)-Tyrc-2J/J] using the reagent concentrations listed in Table S1 ...
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bioRxiv - Bioengineering 2021Quote: ... the Spike protein (Addgene plasmid # 145032) was cloned downstream of the TRE3G promoter ...
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bioRxiv - Neuroscience 2020Quote: Stereotaxic injection of AAV-EF1a-DIO-HChR2(H134R)-eYFP (serotype 2/1 or 2/9, U. Penn Vector Core, Philadelphia, PA, USA or Addgene, Watertown, MA, USA) was performed in 6-8 weeks old DAT-IRES-Cre or FoxP2-Cre transgenic mice ...
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bioRxiv - Bioengineering 2021Quote: ... We acquired psPAX2 encoding lentiviral packaging proteins and PMD2.G encoding a lentiviral envelop protein from Addgene (plasmid no ...
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bioRxiv - Microbiology 2020Quote: ... 36 ug of pRSV-Rev packaging plasmid (containing Rev, Addgene), 18 ug of pMD2.g packaging plasmid (containing VSV-G envelope ...
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bioRxiv - Plant Biology 2021Quote: ... coli strains containing pAC-BETA-At (Addgene plasmid no. #53288), pAC-ZETA (Addgene plasmid no ...
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bioRxiv - Genetics 2020Quote: ... we co-transfected a d2EGFP-containing transfer plasmid (Addgene 138152) with accessory plasmid psPAX2 (Addgene 12260 ...
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bioRxiv - Biochemistry 2020Quote: ... coli BL21-CodonPlus (DE3) cells containing pET28a-MSP1D1delltaH5 vector (Addgene cat# 71714 was kindly purchased by Dr ...
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bioRxiv - Cancer Biology 2020Quote: ... lentiCRISPRv2 (containing hSpCas9 and the chimeric guide RNA cassettes; Addgene), was digested using BsmBI ...
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bioRxiv - Immunology 2021Quote: ... Two sgRNA-containing pX330 plasmids (pSpCas9; Addgene, plasmid ID 42230) were constructed ...
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bioRxiv - Molecular Biology 2023Quote: ... mscv2.2-IRESGFP retroviral vectors containing murine Nlrc4 (Addgene plasmid #60199) or human NLRC4 were used ...
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bioRxiv - Bioengineering 2023Quote: Bacterial stabs containing Sortase 5M were obtained from Addgene (#51140), deposited by the Ploegh lab.90 Individual colonies were picked from plated stabs for 10mL plasmid preps (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA oligonucleotides containing sgRNAs were cloned into LentiCRISPRv2 (Addgene # 52961) using BsmBI ...
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bioRxiv - Cancer Biology 2023Quote: ... the attb-containing part of pX28 plasmid backbone (#46850, Addgene) was PCR amplified ...
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bioRxiv - Systems Biology 2023Quote: ... and an envelope plasmid containing VSV-G (pMD2.G, Addgene). Media was changed 6-8 hours after transfection ...
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bioRxiv - Neuroscience 2023Quote: ... Plasmids containing gene sequences for EphA4 (pDONR223-EPHA4, Addgene, 23919) and EphB3 (FUW-ubiquitin-EphB3-SV40-GFP ...
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bioRxiv - Bioengineering 2023Quote: ... and/or an MS2 loop containing plasmid backbone (Addgene #61424) digested with Bbs1 and treated with alkaline phosphatase (Thermo ...