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Citations for Addgene :
2001 - 2050 of 2862 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... 3T3 cells were transfected with the gRNA-expressing plasmids and the Cas9-expressing plasmid hCas9 (Addgene #41815) using Lipofectamine LTX reagent (Thermo Fisher #15338100 ...
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bioRxiv - Cancer Biology 2019Quote: U2OS-EJ7 cells were infected with viruses produced in HEK293T using second generation vectors psPAX2 (Addgene #12259) as packaging plasmid ...
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bioRxiv - Bioengineering 2019Quote: GV-expressing cells were produced by transforming a pET28a plasmid containing the arg1 gene cluster12 (Addgene #106473) into BL21(A1 ...
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bioRxiv - Cell Biology 2019Quote: ... AICSDP-1:PXN-EGFP was a gift from The Allen Institute for Cell Science (Addgene plasmid 87420).
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bioRxiv - Bioengineering 2020Quote: ... either of the two cell types was subjected to Lipofectamine transfection with CMV-R-GECO1.2-mCherry (Addgene, Watertown ...
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bioRxiv - Bioengineering 2020Quote: VSV-G pseudotyped lentivirus was produced via transfection of HEK 293T cells (ATCC) using psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259) ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were infected to express genetically encoded multimeric nanoparticles (GEMs) using lentiviral construct from Addgene (Plasmid #11693416). Cells were FACS sorted to purify population of cells expressing GEMs.
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bioRxiv - Cell Biology 2021Quote: HEK-293T knockout cell lines were generated by transient transfection of pSpCas9(BB)-2A-Puro (Addgene 48139) encoding U6 driven expression of sgRNAs (Scramble Guide ...
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bioRxiv - Microbiology 2020Quote: Huh7.5.1-Cas9 cells were generated by lentiviral transduction with lentiCas9-blast (Addgene, #52962, gift from Feng Zhang) and subsequently selected with blasticidin for 7 days ...
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bioRxiv - Microbiology 2021Quote: ... A375-dCas or HeLa-dCas cells were generated by transducing with pLenti-dCas9-VP64-Blast (Addgene, #61425). After 7 days of blasticidin selection ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by transformation into competent cells according to an online protocol (GeCKO library Amplification Protocol from Addgene). Afterwards ...
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bioRxiv - Cell Biology 2020Quote: ... pLKO plasmids were co-transfected into HEK293-T cells along with packaging plasmids pMDLg/pRRE (Addgene #12251), pRSV-Rev (Addgene #12253 ...
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bioRxiv - Cell Biology 2021Quote: ... NCI-H1573 and NCI-H1299 cells were transfected with LeGO lentiviral particles (Addgene plasmids #27338, #27339, #27340) (Weber et al. ...
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bioRxiv - Cancer Biology 2022Quote: T98GIRF3-/- and T98GCTRL cell lines were generated using the LentiCRISPRv2GFP plasmid (gift from David Feldser; Addgene # 82416). IRF3 or control guide RNAs were inserted into the LentiCRISPRv2GFP plasmid following provided guidelines and lentiviral particles generated ...
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bioRxiv - Molecular Biology 2022Quote: ... 2014) was co-transfected into 293FT cells with the lentiviral packaging plasmids psPAX2 and pMD2.G (Addgene). Viral production was accomplished as described previously (Joung et al. ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were transduced with floxed jGCaMP7b (AAV1-syn-FLEX-jGCaMP7b-WPRE; Addgene #104493, MOI = 5×105 vg) [14] and low-titer Cre (AAV9-hSyn-Cre-WPRE-hGH ...
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bioRxiv - Cell Biology 2022Quote: Lentivirus stocks were produced in Hek293T cells using pCMVΔR8.91 as packaging plasmid and pMD2.G (Addgene #12259) as vesicular stomatitis virus G glycoprotein (VSV-G ...
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bioRxiv - Microbiology 2022Quote: ... Early passage HEK293T cells were transfected with 1.5 μg of dCas9-KRAB-MeCP2 repressor plasmid (Addgene #110824) and 0.5 μg of Piggyback Transposase (gift from Maxim Greenberg) ...
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bioRxiv - Microbiology 2022Quote: Hela-H1 cells were transfected with a plasmid encoding the VSV-G gene (pMD2.G; Addgene #12259) using Lipofectamine 2000 ...
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bioRxiv - Cell Biology 2022Quote: ... Lentiviruses were transfected into HEK293T cells in combination with viral packing and envelope plasmids pSPAX (Addgene 12260) and pMD2.G (Addgene 12259) ...
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bioRxiv - Biophysics 2022Quote: ... cells constitutively expressing CheRiff were transiently transfected with pCAG-Kir2.1-T2A-tdTomato (from Massimo Scanziani, Addgene 60598) using CalFectin transfection reagent (SignaGen Laboratories ...
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bioRxiv - Biophysics 2022Quote: ... These cells (1.5 × 106) were transfected with 2 μg of plasmid encoding eGFP-Cre recombinase (Addgene # 11923), a gift from Brian Sauer (Le et al. ...
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bioRxiv - Neuroscience 2020Quote: ... for imaging hippocampal pyramidal cells or pAAV-mDlx-GCaMP6f-Fishell-2 (a gift from Gordon Fishell, Addgene plasmid # 83899 ...
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bioRxiv - Molecular Biology 2020Quote: ... For generation of the RPE-1-H2B-mEGFP-FKBP stable cell line the H2B-mEGFP (Addgene #105528) and FKBP (Promega N201A ...
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bioRxiv - Neuroscience 2021Quote: ... 3 × 106 HEK293T cells were transfected using GenJet transfection reagent (Signagen) with 7.5 µg psPax2 (Addgene #12260), 5 µg VSV-G (pMD2.G ...
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bioRxiv - Developmental Biology 2022Quote: NPC-iPS cells (clone 16-13(Maetzel et al., 2014)) were targeted with AAVS1-tdTomato (Addgene 159275) as described before(Ma et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... the transfer plasmid was co-transfected into HEK-293T cells with the packaging plasmids pVSVg (Addgene, #8454) and psPAX2 (Addgene ...
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Simplified Methodology for a Modular and Genetically Expanded Protein Synthesis in Cell-Free SystemsbioRxiv - Synthetic Biology 2019Quote: The bacterial strain used for the preparation of all cell extracts is the C321ΔprfA strain (Addgene #48998) carrying a pEVOL plasmid ...
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bioRxiv - Developmental Biology 2019Quote: SAM mouse embryonic stem cells (ESCs) were generated by lentiviral transduction of lenti dCas9-VP64_Blast (Addgene 61425) and lenti MS2-p65-HSF1_Hygro (Addgene 61426 ...
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bioRxiv - Genetics 2019Quote: ... HEK293T cells were transfected with a mixture of 100 ng of plasmid encoding sgRNA (pRG2; Addgene #104174) and 100 ng of plasmid encoding SpCas9 (pRGEN-Cas9-CMV/T7-Puro-RFP ...
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bioRxiv - Genetics 2019Quote: ... To generate stable 786-O-dCas9-KRAB expressing cell line the Lenti-dCas9-KRAB-blast (Addgene #89567) was used ...
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bioRxiv - Cell Biology 2019Quote: ... Lentivirus was packaged in 293FT or TN cells using the pMD2.G and psPAX2 helper plasmids (Addgene plasmids #12259 and #12260 ...
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bioRxiv - Systems Biology 2019Quote: ... and Dnmt3L KI cells were transduced with the Mouse CRISPR Knockout Pooled Library (GeCKO v2) (Addgene, # 1000000052) [48] via spinfection as previously described ...
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bioRxiv - Cancer Biology 2020Quote: CRISPR screen was performed in BT20 cells using the GeCKO v2 two vector system (Addgene, CAT# 1000000049). Both A and B libraries were amplified according to the distributor ...
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bioRxiv - Molecular Biology 2020Quote: ... These cells were spin transfected for 2h at 1000g with 82*10E6 Brunello virus particles (LentiCRISPRv2, Addgene 73179-LV ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were transduced with lentiviral particles produced using vector pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS (Addgene #60904 ...
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bioRxiv - Cancer Biology 2019Quote: ... A375 cells were first transduced with lentiviral particles produced using vector pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP (Addgene #60903 ...
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bioRxiv - Microbiology 2020Quote: ... Lentiviral particles were generated by co-transfection of 293T cells with pLenti6-Dest_neo_ACE2-2A-Bla or pLenti6-Dest_Puro_TMPRSS2 together with pCMVR8.74 (Addgene plasmid 22036) and pMD2.G (Addgene plasmid 12259 ...
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bioRxiv - Microbiology 2019Quote: ... GM-CSF and IL-4 were produced from HEK293 cells transduced with pAIP-hGMCSF-co (Addgene #74168) or pAIP-hIL4-co (Addgene #74169) ...
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bioRxiv - Immunology 2021Quote: ... HEK 293T cells were reverse-transfected with 3 plasmids: psPAX2 (a gift from Didier Trono, Addgene #12260), pEGFP-Vpr (obtained through the NIH HIV Reagent Program ...
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bioRxiv - Immunology 2021Quote: Generation of Scid.adh.2c2-dCas910x-GCN4 CRISPRi cells dCas910x-GCN4 (pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP, Addgene #60904) was lentivirally transduced into Scid.adh.2c2 cells ...
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bioRxiv - Cancer Biology 2021Quote: ... Lenti- sgRNA/Cre vectors were individually co-transfected into 293T cells with pCMV-VSV-G (Addgene #8454) envelope plasmid and pCMV-dR8.2 dvpr (Addgene #8455 ...
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bioRxiv - Cell Biology 2020Quote: ... 400,000 U2OS cells were transiently co-transfected with 200 ng gRNA expression plasmid (cloned into Addgene 43860), 500 ng Cas9 expression plasmid (Addgene 43945) ...
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bioRxiv - Microbiology 2019Quote: ... These cytokine-conditioned media were produced from HEK293 cells stably transduced with pAIP-hGMCSFco (Addgene no. 74168) or pAIP-hIL4-co (Addgene no ...
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bioRxiv - Microbiology 2020Quote: ... lentiviruses were produced by co-transfecting HEK293T cells with a plasmid encoding VSV-G (Addgene cat#12259), a lentiviral Gag-Pol packaging plasmid (Addgene cat#8455) ...
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bioRxiv - Molecular Biology 2021Quote: ... RPE1-hTERT cells were co-transfected with either of these vectors and the hCas9 vector (Addgene #41815) using a Neon Transfection system (Thermo ...
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bioRxiv - Molecular Biology 2021Quote: ... The RPE1-hTERT TP53-/- RNF8-/- Cas9 cell line was generated by electroporation of phU6-gRNA (Addgene #53188) plasmids expressing sgRNAs (5’-CCCAGAGTCTAAATGGTGTT-3’ and 5’-GGAAGAGGAACAGCATCTTC-3’) ...
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bioRxiv - Cell Biology 2021Quote: ... Lentivirus packaging was performed by using MaxPEI-based co-transfection of HEK293T cells with psPAX2 (Addgene #12260), pMD2.G (Addgene ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were transfected using Lipofectamine 2000 (Thermo) with the pSpCas9(BB)-2A-puro (px459) V2.0 plasmid (Addgene) containing CRISPR guide-RNAs (gRNAs ...
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bioRxiv - Cancer Biology 2022Quote: ... 293T cells were transfected with retroviral or lentiviral transfer plasmid and packaging vector (retrovirus: pCL-Eco, Addgene, 12371 ...