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Citations for Addgene :
2151 - 2200 of 2862 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... the cells were co-transfected with siRNA and 1 µg of plasmid encoding Perceval HR (Addgene ID:49083) at around 50% cell confluency ...
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bioRxiv - Cancer Biology 2020Quote: ... Lentivirus was produced by transfection of HEK293T cell line with psPAX2 (a gift from Didier Trono, Addgene #12260), pMDG.2 (a gift from Didier Trono ...
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bioRxiv - Synthetic Biology 2020Quote: Lentivirus production was performed using HEK-293FT cells and second-generation helper plasmids MD2.G (Addgene plasmid #12259) and psPax2 (Addgene plasmid #12260) ...
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bioRxiv - Microbiology 2019Quote: ... 1.5 million fibroblast cells were transfected with the homologous recombination donor plasmid and an helper plasmid (Addgene #64221) (21) ...
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bioRxiv - Cell Biology 2019Quote: HT1080 cells were transfected with Active WNT3A-V5 (G-8) and Active WNT4-V5 (G-10) from Addgene kit #1000000022 ...
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bioRxiv - Cancer Biology 2020Quote: D10 and SK-MEL-23 melanoma cells were first transduced to stably express Cas9 (lentiCas9-Blast, Addgene, #52962) and selected with blasticidin (5 μg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... The J8 clonal cell line was infected with lentiCRISPR v2 (a gift from Feng Zhang; Addgene plasmid # 52961), lacking or containing guide RNA against BCAR3 ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa R19 cells stably expressing mCherry-EGFP-LC3 were obtained through stable transfection of plasmid DNA (Addgene #22418), followed by puromycin selection and generation of monoclonal cell populations by limited dilution ...
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bioRxiv - Cell Biology 2021Quote: Measurement of NFAT4 nuclear translocation in HEK293 cells was achieved by transient overexpression of NFAT4-GFP (Addgene #21664) as previously described(30 ...
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bioRxiv - Cell Biology 2020Quote: MCF10A cells were transfected via PEI (https://www.addgene.org/protocols/transfection/) with pSpCas9(BB)-2A-GFP (PX458) - (Addgene #48138) carrying sgRNA for hSLC9A1 KO 5’-GTTTGCCAACTACGAACACG (SLC9A1:HGLibA_45399 ...
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bioRxiv - Biochemistry 2021Quote: ... Electrocompetent BW25113 cells were co-transformed with the pORTMAGE-4 plasmid (Addgene plasmid #72679, courtesy of Csaba Pál) and the pBAD-yTrm5 plasmid ...
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bioRxiv - Cancer Biology 2020Quote: HEK293T cells at 70% confluence were transfected with 10 μg of pMRX-IP-GFP-LC3-RFP (#84573, Addgene), 5 μg of VSV.G (#14888 ...
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bioRxiv - Neuroscience 2021Quote: AAV1/2.hSyn-GFP particles were generated by co-transfection of HEK293T cells with AAV2/1 (Addgene 112862), AAV2/2 (Addgene 104963) ...
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bioRxiv - Immunology 2021Quote: ... the RBD expression vectors were co-transfected into Expi293F cells together with the secreted BirA-Flag plasmid (Addgene) in the presence of 100 μM biotin.
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bioRxiv - Genomics 2021Quote: ... These cells were co-transfected with lentiviral packaging plasmids like VSVG (a gift from Bob Weinberg, Addgene #8454) and PAX2 (a gift from Didier Trono ...
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bioRxiv - Cell Biology 2020Quote: ... Linearized pShuttle-CMV plasmids were transformed into the final viral backbone using electrocompetent AdEasier-1 cells (Addgene, #16399). Successful incorporation of pShuttle-CMV construct into AdEasier-1 cells confirmed via digestion with PacI (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... were injected with a viral vector specifically targeting GCaMP6f expression to CaMKII-positive cells (AAV5.CamKII.GCaMP6f.WPRE.SV40, Penn Vector Core via Addgene, CS1024). GCaMP6f injections were targeted at the following coordinates ...
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bioRxiv - Biophysics 2019Quote: ... SUM-159 cells genome edited to express σ2-EGFP [2] and transiently expressing mRuby-CLTB (Addgene; Plasmid #55852) were cultured in F-12 medium with hydrocortisone ...
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bioRxiv - Molecular Biology 2020Quote: ... The TRIPZ plasmid was transfected into 293T cells along with lentiviral packaging and envelope 2nd generation plasmids (Addgene packaging 11263 ...
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bioRxiv - Cell Biology 2021Quote: MEF cell lines were transduced with lentivirus containing Cas9 and guide RNA in the lentiCRISPRv2 plasmid (Addgene, 52961) and selected using 2 μg/ml puromycin ...
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bioRxiv - Immunology 2021Quote: ... Cells were co-transfected with either plasmid #166856 or #166857 and a plasmid encoding BirA ligase (Addgene #32408) at a 4:1 ratio (m/m) ...
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bioRxiv - Cell Biology 2019Quote: ... cells expressing endogenous GFP-CLIC4 were transfected with Cry2-VHH Addgene #58370) and Tom20-CIB-stop (Addgene #117243). Cells were kept in the dark and incubated for 24-48 hours ...
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bioRxiv - Genetics 2020Quote: ... Cas9 activity was assessed by transducing parental Vero-E6 or Vero-E6-Cas9 cells with pXPR_047 (Addgene 107645), which expresses eGFP and an sgRNA targeting eGFP (72) ...
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bioRxiv - Molecular Biology 2022Quote: ... or CALU6 and H1975 cells were infected with a metabolism focused CRISPR library (Addgene,(Birsoy et al., 2015)) ensuring a multiplicity of infection ∼0.3 following 3 days puromycin selection ...
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bioRxiv - Developmental Biology 2022Quote: Lentiviruses were produced in HEK 293T cells by cotransfection of lentiviral constructs with third generation packaging plasmids (Addgene) for 48–72 hr ...
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bioRxiv - Cell Biology 2022Quote: ... YAP–HaloTag CRISPR knock-in U-2 OS cells were transfected with pEGFP-C3-Lats1 (Addgene plasmid # 19053) or pEGFP C3-Mst2 (Addgene plasmid # 19056) ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells were used to package lentiviruses by co-transfecting viral packaging plasmids pCMV-VSV-G (Addgene #8454), psPAX2 (Addgene #12260 ...
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bioRxiv - Molecular Biology 2023Quote: ... which had been produced in HEK293T cells by transiently co-transfecting the lentiviral packaging plasmid psPAX2 (Addgene #12260), a lentiviral vector encoding μ-OR-SmBiT (pLenti-Blast Addgene #17451) ...
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bioRxiv - Microbiology 2023Quote: The drug selection cassettes in the CN cell line were excised using transient expression of Cre recombinase from pLEW100Cre_del_tetO (Addgene plasmid 24019 ...
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bioRxiv - Molecular Biology 2023Quote: ... MEFs cells were infected with a pLKO.1-Puro plasmid encoding a scrambled shRNA sequence (Addgene plasmid #1864). A list of the shRNA sequences is provided in Supplementary Table 2.
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bioRxiv - Neuroscience 2022Quote: ... Lentivirus was created from this plasmid by co-transfecting HEK293T cells with this plasmid alongside psPAX2 (Addgene #12260) and MD2.G (Addgene #12259) ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293T WT cells were transfected using Lipofectamine3000 with pLenti plasmids and lentiviral packaging plasmids (Gag-pol (Addgene #14887), VSV-G (Addgene #8454) ...
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bioRxiv - Neuroscience 2022Quote: ... Lipofectamine3000 was used to transfect cells with 0.5ug of pSpCas9(BB)-2A-GFP(PX458) targeting plasmid (Addgene # 48138) containing sgRNA sequences targeting full-length or truncations in SRRM2 and PNN (Supplemental Table 3) ...
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bioRxiv - Molecular Biology 2022Quote: ... Synthetic cell type specific promoters were obtained from the following Addgene plasmids: 161_pAAV-ProD5-CatCh-GFP-WPRE (Addgene plasmid # 125981 ...
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bioRxiv - Genomics 2022Quote: Twenty million cell batches of J1 ESCs were transfected each with 20 μg dCas9-VPR vector (Addgene #63798) and 20 μg plasmid constructed by the IGBMC molecular biology platform (available on request) ...
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bioRxiv - Neuroscience 2024Quote: ... cells were transfected with either (G4C2)92 and (G4C2)2 lentiviral transfer plasmids along with PAX (Addgene #12260) and VSV-G (Addgene #12259 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HEK-293 cells were transfected with two plasmid vectors CMV–R-GECO1 (a gift from Robert Campbell, Addgene plasmid #32444 ...
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bioRxiv - Microbiology 2024Quote: ... N/Tert-1 Cas9 knockout cells were generated by transduction with a spCas9 lentiviral expression vector (Addgene # 52962) and selecting with blasticidin ...
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bioRxiv - Molecular Biology 2024Quote: ... the HEK293T cells were transfected with the respective constructs along with envelope plasmid (pMD2.G) (Addgene, Cat# 12259), and packaging plasmid (psPAX2 ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were reprogrammed by a mixture of retroviral vectors encoding OCT3/4 (pMXs-hOCT3/4 Addgene: 17217), SOX2 (pMXs-hSOX2 Addgene ...
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bioRxiv - Molecular Biology 2024Quote: ... MutuI cells were transduced with lentiparticles derived from the doxycycline inducible Cas9 vector pCW-Cas9 (Addgene Plasmid #50661) and selected for stable integration with 2 μg/mL puromycin ...
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bioRxiv - Cell Biology 2024Quote: B16-F1 cells were transiently transfected with CYRI-B-p17-GFP and mCherry-β1 integrin (Addgene plasmid #55064) and plated on laminin coated glass bottom dishes ...
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bioRxiv - Cell Biology 2023Quote: ... we measured intramitochondrial free Ca2+ levels by transfecting AML12 cells with the CMV-mito-GEM-GECO1 plasmid (Addgene, 32461 ...
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bioRxiv - Genetics 2023Quote: ... The HAP1 cells used in SMuRF were immortalized using lentivirus packaged with pLV-hTERT-IRES-hygro (Addgene, 85140), a gift from Tobias Meyer ...
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bioRxiv - Cell Biology 2024Quote: ... AICSDP-82:SON-mEGFP was a gift from Allen Institute for Cell Science (Addgene plasmid # 133964; http://n2t.net/addgene:133964; RRID:Addgene_133964). AICSDP-42:AAVS1-mTagRFPT-CAAX was a gift from Allen Institute for Cell Science (Addgene plasmid # 107580 ...
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bioRxiv - Cell Biology 2024Quote: ... AICSDP-42:AAVS1-mTagRFPT-CAAX was a gift from Allen Institute for Cell Science (Addgene plasmid # 107580; http://n2t.net/addgene:107580; RRID:Addgene_107580). APEX2-NLS ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were immortalized by transduction with a retrovirus expressing SV40 large T antigen from pBABE-puro largeTcDNA (Addgene plasmid # 14088 ...
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bioRxiv - Cell Biology 2023Quote: ... HEK-293T cells seeded into 12 well plates were transfected with the retroviral packaging plasmid pUMVC (Addgene: 8449), pLXIN (Clontech ...
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bioRxiv - Bioengineering 2023Quote: ... Lentiviruses were packaged using 293FT cells by co-transfection of lentiviral transfer plasmids with pMD2.G (Addgene #12259) and psPAX2 (Addgene #12260 ...
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bioRxiv - Cancer Biology 2023Quote: Retroviral supernatants were prepared by co-transfecting 293T cells (DSMZ, cat ACC635) using pCL ECO (Addgene, cat 12371) for mouse cells or pCL ampho for human cells and a retroviral vector ...