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Citations for Addgene :
151 - 200 of 985 citations for Two Hybrid cDNA Library since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... Two DNA fragments were independently amplified with two primer sets (NLS_Fw1/Rv1, NLS_Fw2/Rv2) using pDest_pK7WG2_pRPS5A_CTP OleGFP (Addgene #171723) as a PCR template ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were co-transfected with two gRNAs and pSpCas9n(BB)-2A-Puro (PX462) V2.0 (Addgene, 62987). After 24 h the cells were selected with puromycin and expanded ...
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bioRxiv - Biochemistry 2023Quote: Two vectors with C-terminal MBP (maltose-binding protein)-His6 Tag (2Cc-T; Addgene Plasmid #55209) or N-terminal His10-MBP Tag (2CT-10 ...
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bioRxiv - Cancer Biology 2023Quote: ... The two sgRNA sequences (Suppl, Table S2) were cloned into the pLentiguide-PURO plasmid (Addgene, #52963). The plasmid pLentiCRISPR-mCherry (Addgene ...
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bioRxiv - Genomics 2023Quote: ... HEK293T cells were co-transfected with two packaging plasmids (pCMV-VSV-G and delta8.9, Addgene #8454) and either a desired transfer plasmid ...
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bioRxiv - Immunology 2023Quote: ... The two sgRNAs were cloned into the expression vector px330 carrying the cas9 gene (Addgene 42230), and then the two px330 plasmids with different sgRNAs were mixed equally at a final concentration of 2.5 ng/μL and injected into fertilized eggs of B6D2F1 × B6 by the microinjection technique ...
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bioRxiv - Microbiology 2023Quote: The eSpCas9(1.1) gene containing two nuclear localization signals (NLS) was obtained from Addgene (Plasmid #71814) and cloned into the pST32 vector ...
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bioRxiv - Immunology 2023Quote: ... The gRNA construct was then co-transfected with two packaging plasmids (pMD2.G and pSPAX2, Addgene) into HEK293T cells to produce lentiviruses ...
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bioRxiv - Cell Biology 2019Quote: Human GeCKOv2 CRISPR knockout pooled library (Pooled Library #1000000048)35 and pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene plasmid # 42230)97 were gifts from Feng Zhang ...
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bioRxiv - Immunology 2020Quote: ... A plasmid library of sgRNAs targeting all protein coding genes in the mouse genome (Brie Knockout library, Addgene 73633) was packaged into lentivirus using HEK293T cells ...
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bioRxiv - Cell Biology 2023Quote: Human genome targeting CRISPR Knock-Out (GeCKO) v2 lentiviral pooled libraries (A and B libraries) were purchased from Addgene and amplified according to a published protocol (Sanjana et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmids constructs pSBbi-RN-FGF19 and pSBbi-BB-FGF15 were respectively generated by cloning the human FGF19 amplified from Huh7 cDNA and the mice Fgf15 amplified from ileum cDNA onto the pSBbi-RN (Addgene #60519) and pSBbi-BB (Addgene #60521 ...
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bioRxiv - Molecular Biology 2021Quote: 144 million Lig4-/- abl pre-B cells were transduced with a viral tet-inducible guide RNA library (Pooled Library #67988, Addgene) containing 90,000 gRNAs targeting over 18,000 mouse genes ...
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bioRxiv - Immunology 2021Quote: The lentiviral gRNA plasmid library for genome-wide CRISPR-Cas9 screen (Mouse Improved Genome-wide Knockout CRISPR Library v2, Pooled Library #67988#) and mock vector (#67974) was obtained from Addgene. The library was amplified following the protocol provided by Addgene ...
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bioRxiv - Genetics 2023Quote: ... it was expanded and 100 million cells were infected with a BFP-expressing gRNA lentiviral library (Addgene Pooled Library #67988) at an infection rate of 25-30% to keep the Multiplicity of Infection (MOI ...
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bioRxiv - Microbiology 2023Quote: Library virus was generated and titrated as described above using the Brunello sgRNA pool library in lentiGuide-Puro (Addgene, 73178). In all cases ...
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bioRxiv - Cancer Biology 2021Quote: ClonTracer Barcoding Library 43 was purchased from Addgene. The plasmid pool was expanded by electroporation transformation ...
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bioRxiv - Cancer Biology 2021Quote: The Brunello sgRNA pooled plasmid library (Addgene #73178) and lentiCas9-Blast plasmid (Addgene #52962 ...
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bioRxiv - Genomics 2020Quote: ... The GeCKO A pooled sgRNA library (Addgene #1000000049) was used for gene knock out screening and the SAM pooled sgRNA library (Addgene #1000000057 ...
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bioRxiv - Cancer Biology 2020Quote: ... The GeCKO v2 library was purchased from Addgene (2-plasmid system catalog number 1000000049) ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: Human GeCKOv2 CRISPR knockout pooled library (Addgene # 1000000049) and lenti-Cas9 plasmid were obtained from addgene (Addgene # 52962) ...
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bioRxiv - Cell Biology 2020Quote: ... following the CRISPR library-related instructions (#75316, Addgene). All PCRs were pooled and sequenced by Illumina sequencing (Duke Center for Genomic and Computational Biology (GCB) ...
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bioRxiv - Cell Biology 2022Quote: ... mouse GeCKOv2 CRISPR knockout pooled library (Addgene #1000000053,18), pMD2.G and psPAX2 were transfected into Lenti-X 293T cells ...
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bioRxiv - Cancer Biology 2022Quote: ... The library was obtained from Addgene (73178-LV). In brief ...
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bioRxiv - Cell Biology 2022Quote: ... CRISPR sgRNA library lentiGuide-Puro-Brie (Addgene, #73633), which contains a pool of 78,637 sgRNAs targeting 19,647 genes was transduced into mESCs with MOI < 0.3 and an average of 400x coverage ...
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bioRxiv - Microbiology 2023Quote: The human SAM CRISPRa sgRNA library (Addgene #1000000078) was cloned into the pHW-TRPPC-NS rescue plasmid backbone for PR8 (Fig S2A ...
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bioRxiv - Cancer Biology 2023Quote: ... The Brunello pooled library of gRNAs (Addgene #73178) was kindly provided by David Root ...
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bioRxiv - Cell Biology 2022Quote: ... 2018 (Dolcetto CRISPRi library set A, Addgene #92385). This library contains 57,050 sgRNA ...
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bioRxiv - Cancer Biology 2023Quote: ... we used human decipher module 1 library (RRID:Addgene_28289)(Diehl et al ...
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bioRxiv - Cancer Biology 2024Quote: ... The DNA Damage Response MKOv4 Library (Addgene, #140219) was from our previous study35.
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bioRxiv - Cancer Biology 2024Quote: ... This library was cloned into pRC0162 (Addgene #195319) and sequence verified ...
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bioRxiv - Cell Biology 2024Quote: ... The library was purchased from Addgene (Cat#1000000083) and amplified using the protocol provided by Addgene ...
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bioRxiv - Developmental Biology 2020Quote: ... was seeded on MEF then transfected with two designed pSpCas9(BB)-2A-Puro (pX459) V2.0 (Addgene #62988) plasmids using Lipofectamine 3000 (Thermofisher) ...
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bioRxiv - Developmental Biology 2022Quote: ... a solution containing two sgRNAs (40 ng/μL each) and Cas9 protein (250 ng/μL) (Addgene; 47327) (Gagnon et al. ...
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bioRxiv - Developmental Biology 2019Quote: ... Two guide RNAs were cloned into the BbsI site of pU6-BbsI-chiRNA plasmid (obtained from Addgene) and injected by Best Gene into w1118 ...
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bioRxiv - Bioengineering 2020Quote: ... either of the two cell types was subjected to Lipofectamine transfection with CMV-R-GECO1.2-mCherry (Addgene, Watertown ...
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bioRxiv - Cancer Biology 2020Quote: Single knockouts were generated either using a two-vector Streptococcus pyogenes (Sp) Cas9 system: LentiV_SpCas9_puro (Addgene, 108100) and LRG2.1 backbone (Addgene ...
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bioRxiv - Biophysics 2020Quote: ... Two plasmids containing full-length untagged Npl4 and C-terminal His-tagged Ufd1 were purchased from Addgene. Npl4 fragments were expressed in pET-28a vector with an N-terminal His-SUMO tag ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting DNA fragment containing two MpU6promoter-gRNA cassettes was transferred into pMpGE010 (cat. no. 71536, Addgene) (Sugano et al. ...
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bioRxiv - Cancer Biology 2020Quote: CRISPR screen was performed in BT20 cells using the GeCKO v2 two vector system (Addgene, CAT# 1000000049). Both A and B libraries were amplified according to the distributor ...
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bioRxiv - Genetics 2021Quote: ... The two gRNAs were respectively sub-cloned into the pGL3-U6-sgRNA-PGK-puromycin (Addgene plasmid # 51133) plasmid and PX330-mcherry (Addgene plasmid #98750 ...
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bioRxiv - Biochemistry 2021Quote: A 20 μL reaction containing 150 ng of a plasmid containing two loxP sites (Addgene Plasmid #26852) and 500 nM of Cre or Cre mutants in recombination buffer (50 mM Tris-Cl ...
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bioRxiv - Microbiology 2022Quote: ... Two constructs for AdgI were generated by subcloning to vectors pET28-SUMO and 13S-A (Addgene, 48323) vectors ...
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bioRxiv - Synthetic Biology 2022Quote: ... Gypsy insulator elements were subsequently cloned into PRE-Hsp70BbCas9_1.0 through two Gibson cloning events to generate PRE-Hsp70BbCas9_1.2 (Addgene 190796) with one gypsy insulator element (KpnI digest ...
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bioRxiv - Neuroscience 2023Quote: ... Control mice were injected with one of two control viruses: AAV9-hSyn-DIO-mCherry (AddGene Plasmid #50459), virus titer 2.17 x 1013 or AAV9-hSyn.HI.eGFP-Cre-WPRE-SV40 ...
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bioRxiv - Neuroscience 2022Quote: ... for optogenetic control: Two 400 nl injection of AAV2retro-GAG-ChR2 (Addgene 28017, 7 × 1012 VG/ml) or AAV2retro-CaMKIIa-stGtACR2-FusionRed (Addgene 105669 ...
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bioRxiv - Plant Biology 2023Quote: ... These two level 1 vectors were assembled with the Kanamycin resistance gene (pNOS::NPTII-OCST; Addgene #51144), the AtCas9 (2×35S::AtCAS9-OCST ...
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bioRxiv - Cell Biology 2024Quote: ... The two sgRNAs were cloned into either the pSpCas9(BB)-2A-GFP (PX458) plasmids (Addgene, Cambridge, USA)31 ...
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bioRxiv - Synthetic Biology 2024Quote: ... the two fragments of FlipGFP B1-9 and B10-E5-B11-TEVcs-K5 were amplified from Addgene Plasmid #124429 via PCR ...
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bioRxiv - Molecular Biology 2023Quote: We designed the following two plasmids based on the pCDH-EF1a-eFFly-mCherry backbone vector (Addgene #104833) with cloning sites BmtI and BamHI ...