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Citations for Addgene :
1 - 50 of 985 citations for Two Hybrid cDNA Library since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... elegans cDNA library into pACT2.2 (Addgene), respectively ...
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bioRxiv - Molecular Biology 2024Quote: Mouse Two Plasmid Activity-Optimized CRISPR Knockout Library (Addgene#1000000096) was amplified in E coli ...
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bioRxiv - Genetics 2023Quote: ... the rep/cap hybrid plasmid pAAV2/8 (Addgene #112864 ...
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bioRxiv - Developmental Biology 2023Quote: Full length fmi cDNA from UAS-fmi (ref 7) was subcloned in two EcoR1-Xho1fragments into pQUAST (#24349; Addgene) and transformed into w1118 flies to generate random genomic insertions.
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bioRxiv - Cell Biology 2023Quote: ... elegans cDNA library and cloned into UC Berkeley Macrolab vector 2-CT (Addgene #29706), which encodes a TEV protease-cleavable His6-MBP (maltose binding protein ...
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bioRxiv - Developmental Biology 2022Quote: ... and pLV overexpression constructs were obtained by co-transfection of library plasmids with two viral packaging plasmids psPAX2(RRID:Addgene_12260) and pMD2.G (RRID:Addgene_ 12259 ...
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bioRxiv - Microbiology 2021Quote: The lentiviral particles expressing individual cDNA labelled with an mCherry marker were generated by co-transfection of the cDNA plasmid mixture with two lentiviral packaging plasmids pR8.74 and pVSV-G (Addgene, 12259) as a proportion of 10:10:1 into HEK293T cells ...
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bioRxiv - Cell Biology 2023Quote: ... the lentiviral vectors encoding each SLX4 cDNA (kindly provided by Dr. Minoru Takata) were individually co-transfected with two assistant vectors pMD2.G (#12259, Addgene) and psPAX2 (#12260 ...
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bioRxiv - Cell Biology 2020Quote: ... NSC cells were plated into T225 flasks at an appropriate density such that each replicate had 250-500-fold representation, using the two previously published CRISPR-Cas9 libraries (Doench et al, 2016; Shalem et al, 2014) (Addgene) or a custom synthesized sgRNA library (Twist Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... The library lentivirus was generated by co-transfection of the library plasmid mixture with two lentiviral packaging plasmids pR8.74 and pVSV-G (Addgene, 12259) as a proportion of 10:10:1 into HEK293T cells ...
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bioRxiv - Cell Biology 2020Quote: Approximately 300 × 106 IAPEz reporter cells expressing Cas9 were lentivirally infected with a genome-wide Mouse Two Plasmid Activity-Optimized CRISPR Knockout Library (Addgene #1000000096) as described above ...
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bioRxiv - Microbiology 2019Quote: Single oligonucleotide guides for three scramble controls and two exons in Tax1bp1 selected from the Brie library were closed into lentiGuide puro (Addgene #52963) using the following primer pairs ...
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bioRxiv - Molecular Biology 2023Quote: 1.6 x 108 A375 cells were transduced into thirty-two 150 mm plates with lentivirus carrying The Toronto Knockout Library v3 (TKOv3, Addgene # 90294) in standard culture media + 8 µg/mL polybrene ...
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bioRxiv - Cancer Biology 2023Quote: ... 377 million iCas9-expressing NIH-3T3 cells were transduced with the Mouse Two Plasmid Activity-Optimized CRISPR Knockout Library (Wang et al, 2017) (Addgene; 1000000096) using spinfection at an MOI of 0.3 to ensure a final library coverage of 500X ...
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bioRxiv - Cell Biology 2020Quote: ... was constructed by inserting the cDNA of mkate2-3xGly flanked by two 800 bp homology arms into the entry vector backbone pENTR1a (Addgene Plasmid #17398). The pENTR1a backbone vector was digested with EcoRl-HF and BamHl-HF (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... with two px458 (Addgene, 48138) plasmids containing gRNA sequences targeting the deletion breakpoints ...
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Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistancebioRxiv - Cell Biology 2024Quote: ... The cDNAs for GFP-tagged two FYVE domains of mouse HRS and PH domain of human TAPP1 were obtained from Addgene (#140047 and #161985, respectively) and cloned into the pLVX-IRES-puro vector ...
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bioRxiv - Neuroscience 2023Quote: ... The two helper AAVs from Addgene were diluted in Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Bioengineering 2022Quote: ... two packaging plasmids (Addgene #8454 and 8455). 50,000 HEK293T were transduced with a MOI of 10 of each lentivirus in a 24-well format and then selected for blasticidin expression (5 μg/ml ...
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bioRxiv - Biochemistry 2019Quote: ... Two previously described plasmids pSADuet259 (Addgene #121910) and pSABAD250 (Addgene #45612 ...
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bioRxiv - Plant Biology 2020Quote: ... two gBlocks and four plasmids from Addgene, pICH47742::2x35S-5′UTR-hCas9 (STOP)-NOST (Addgene no ...
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bioRxiv - Neuroscience 2024Quote: ... two plasmid constructs pmGFP10C-Tau (Addgene, #71433) and pmGFP11C-Tau (Addgene ...
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bioRxiv - Cell Biology 2023Quote: ... CRISPRi libraries (single CRISPRi-v2 library, Non-targeting dual library [Addgene #197348] ...
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bioRxiv - Cancer Biology 2022Quote: Wildtype full-length LRP8 was cloned from a MDA-MB-453 complementary DNA (cDNA) library and inserted into pLenti-CMV-GFP-Hygro (Addgene #17446) between XbaI and BamHI ...
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bioRxiv - Neuroscience 2023Quote: ... were cloned by PCR amplification of the full-length human GPCR cDNA library (hORFeome database 8.1) or the PRESTO-Tango GPCR Kit36 (Addgene Kit #1000000068). To optimize the 5-HT sensors ...
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bioRxiv - Cell Biology 2023Quote: ... CRISPRi libraries (single CRISPRi-v2 library, Non-targeting dual library [Addgene #197348], or EMC2 dual library [Addgene #197349]) were transduced at a multiplicity of infection less than one into 300-330 million K562-CRISPRi-Tet-ON cells containing the appropriate reporter ...
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bioRxiv - Genomics 2020Quote: ... Two different plasmids pENO1-iRFP-NATr (Addgene Inc) and pFA-GFP-HIS1 were used to design the repair segment ...
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bioRxiv - Immunology 2021Quote: ... two sgRNA-Cas9 expression vectors (PX458, Addgene # 43138) targeting exon 7 and intron 6 of the CDC42 gene and targeting vector with a floxed puromycin resistance cassette flanked by 2 kb homology arms were introduced into an iPSC clone derived from a healthy control for homologous recombination ...
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bioRxiv - Microbiology 2022Quote: ... in the two lentiviral vector version (Addgene 1000000049), was used (33 ...
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bioRxiv - Biochemistry 2020Quote: ... we amplified the open reading frame from a mouse cDNA library using polymerase chain reaction and inserted it into a pBAD His6 Sumo TEV LIC cloning vector (Addgene Plasmid #37507) that had been engineered to encode an N-terminal Avi tag and C-terminal ybbR tag58 ...
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bioRxiv - Biochemistry 2022Quote: ... To produce recombinant CK2 kinase the CKA2 gene was PCR amplified from yeast cDNA library and cloned into a pOPINF vector (a kind gift from Ray Owens, Addgene plasmid 26042) to encode a 3C-cleavable His6 fusion protein ...
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bioRxiv - Molecular Biology 2021Quote: ... For mutagenesis of the two SIMs in sov two pairs of sgRNAs (1+4 and 3+2) were cloned into pCFD4d (Addgene 83954) (Ge et al. ...
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bioRxiv - Immunology 2023Quote: ... The CRISPR library vectors (Human CRISPR Metabolic Gene Knockout Library; Addgene, Pooled Library #110066) 12 or the single sgRNA vectors ...
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bioRxiv - Cell Biology 2019Quote: ... or hCRISPRi-v2 library (Addgene pooled library #83969) for a pooled genome-wide CRISPRi screen ...
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bioRxiv - Neuroscience 2020Quote: ... with two package plasmids: pCMV-VSV-G (Addgene, #8454) and pCMV-dR8.2 dvpr (Addgene ...
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bioRxiv - Genetics 2022Quote: ... TSCs were transfected with two PX458 plasmids (Addgene, #48138) each containing one single guide RNA (RE79_1/RE79_2 ...
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bioRxiv - Molecular Biology 2022Quote: ... two sgRNAs were cloned into the pCFD6 (Addgene #73915) vector which is a tRNA–sgRNA expression system using the endogenous tRNA processing machinery liberating multiple functional sgRNAs from a single precursor transcript in the nucleus29 ...
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bioRxiv - Genomics 2023Quote: ... and the CRISPRi-v2 library (Addgene Pooled Libraries #83969) (Horlbeck et al. ...
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bioRxiv - Cell Biology 2023Quote: ... cDNAs from Addgene plasmids #111906 and #111905 were PCR amplified ...
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bioRxiv - Neuroscience 2020Quote: ... we made two 100μL injections of AAVrg.CAG.tdTomato (Addgene, #59462-AAVrg) bilaterally into CFA (0.0 mm posterior ...
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bioRxiv - Neuroscience 2020Quote: ... we modified two plasmids procured from Addgene (#62724 and #74291). We digested plasmid #74291 with BamHI ...
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bioRxiv - Cancer Biology 2020Quote: ... Two crRNAs introduced into lentiviral vectors (pLentiCRISPR-E, Addgene #78852) which contains eSpCas9 and puroMYCin cassette.
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bioRxiv - Neuroscience 2021Quote: ... Kim modified two AAV vectors (Addgene plasmid #18917 and #38044). DNA of #18917 was cut with BamHI and Eco RI ...
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bioRxiv - Neuroscience 2020Quote: ... two unc-4 sgRNA plasmids and Cas9 plasmid (Addgene #46168) (Friedland et al. ...
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bioRxiv - Immunology 2019Quote: ... and the two Rictor shRNA vectors were purchased from Addgene. The pEnter/D-TOPO entry vector was purchased from Thermo Fischer ...
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bioRxiv - Immunology 2021Quote: ... Two sgRNA-containing pX330 plasmids (pSpCas9; Addgene, plasmid ID 42230) were constructed ...
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bioRxiv - Molecular Biology 2022Quote: Two plasmids (based on the gRNA expression plasmid Addgene #49330) encoding Cas9 as well as guide RNAs targeting the integration site were kindly received from the Brennecke Lab at IMBA Vienna (Batki et al ...
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bioRxiv - Microbiology 2022Quote: ... Two different plasmids pENO1-iRFP-NATr (Plasmid #129731, Addgene Inc) and pFA-GFP-HIS1 were used to design the repair segments ...
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bioRxiv - Neuroscience 2023Quote: ... and the two helper plasmids pCMV-VSV-G (Addgene #8454) and psPAX2 (Addgene #12260 ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse Ebf1 cDNA (Addgene) was cloned into pLVX Tet-One Puro plasmid (Clontech) ...