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Citations for Addgene :
1901 - 1950 of 1985 citations for Cis Dcca 100 Ug Ml In Acetonitrile D3 1 Carboxyl 13C2 99%; 1 D 97% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... RFP-GFP-LC3-encoding retrovirus (9.3×10 TU/mL, produced at the Molecular Tools Platform at the CERVO Brain Research Center based on plasmid #21074, Addgene, kindly provided by Dr ...
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bioRxiv - Neuroscience 2021Quote: The single-stranded AAV vector ssAAV-9/2-hSyn1-chI-dlox-Igk_(rev)-dlox-WPRE-SV40p(A) (1.2 × 1013 vg/ml) was produced using the plasmid by Viral Vector Facility at University of Zurich (Addgene #121922). For intravitreal viral injections of the AAV ...
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bioRxiv - Neuroscience 2021Quote: ... 1 µL of 4.6 x 1012vg/mL AAV5-hSyn-DIO-hM4D(Gi)-mCherry (UNC Viral Vector Core; (Krashes et al., 2011)) or 7 x 1012vg/mL AAV5-hSyn-DIO-mCherry (Addgene) was injected at 2.1 mm below the surface of the brain (Andrews-Zwilling et al. ...
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bioRxiv - Neuroscience 2022Quote: ... DV: −4.4) we infused 450nL of pAAV5- Ef1a-DIO-(hChR2-E123A)-eYFP (Addgene-ChR2 & eYFP titre: 2.2x10^13 GC/mL)76 ...
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bioRxiv - Neuroscience 2022Quote: ... using an ultra-fine dental drill and inserted a glass pipette backfilled with AAV2-CamkIIa-hChR2(H134R)-EYFP (titer: 3×10¹² viral genomes per ml, 26969-AAV2, Addgene). AC was approached similar to extracellular recordings ...
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bioRxiv - Neuroscience 2022Quote: ... CRF1-cre mice were injected with 500 nL/hemisphere of AAV5-hSyn-DIO-eGFP (50457-AAV5; titer ≥ 7×10¹² vg/mL, Addgene) into the VTA (ML ±0.60 ...
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bioRxiv - Neuroscience 2022Quote: ... AAV9-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (300 nL; 3×1011 GC/ml; Serotype:9; Addgene, Massachusetts, USA) was unilaterally injected into the right MePD using a 2-μL Hamilton micro syringe (Esslab ...
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bioRxiv - Neuroscience 2022Quote: ... titer ≥ 7× 1012 vg/mL; gift from Edward Boyden and purchased through UNC Vector Core; now commercially available from Addgene viral preparation #37825-AAV5 ...
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bioRxiv - Neuroscience 2022Quote: ... titer ≥ 7× 1012 vg/mL; gift from Edward Boyden and purchased through UNC Vector Core; now commercially available from Addgene viral preparation #29777-AAV5 ...
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bioRxiv - Neuroscience 2022Quote: ... unilateral injections of 50-70 nL AAV5-CAG-ArchT-GFP (titer ≥ 7× 1012 vg/mL; gift from Edward Boyden and purchased through UNC Vector Core; now commercially available from Addgene viral preparation #29777-AAV5 ...
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bioRxiv - Neuroscience 2023Quote: ... The following viruses were injected:: AAV5-hSyn-DIO-hM3Dq-mCherry (DREADD-Gq, titer 6 × 1012 cfu/ml, 250ul bilateral, #44361, Addgene), AAV5-hSyn-DIO-mCherry (control ...
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bioRxiv - Neuroscience 2023Quote: ... a 50:50 mix of AAV8-CamKII-mCherry (Neurophotonics, Laval University, Quebec City, Canada, Lot #820, titre 2×1013 GC/ml) and AAVrg-CAG-GFP (Addgene, Watertown ...
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bioRxiv - Neuroscience 2023Quote: ... Agrpcre/wt::Ghsrwt/wtand Agrpcre/wt::Ghsrlox/lox mice were injected with both cre-dependent AAV9-hSyn-FLEX-GCaMP6s-WPRE-SV40 (as above) and cre-dependent AAV5-hSyn-DIO-hM3D(Gq)-mCherry (∼2.0 x 1012 vg/ml; Addgene #44361) bilaterally injected into the ARC to test the functional capacity of AgRP GHSR KO to increase food intake to a ghrelin independent signal ...
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bioRxiv - Neuroscience 2023Quote: The viral construct AAV5-EF1a-DIO-hChR2(H134R)-eYFP-WPRE-HGHpA (Titer=2.1×1013 GC/mL) was obtained from Addgene (#20298). Male and female SST-IRES-Cre+/- mice at least 8 weeks old were deeply anesthetized with 5% isoflurane and underwent stereotaxic surgery ...
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bioRxiv - Neuroscience 2023Quote: Ntsr1-Cre mice (n = 3) were stereotaxically injected with an inhibitory opsin (AAV1-hSyn-SIO- stGtACR2-FusionRed, 5 x 1011 vg/ml, Addgene) see “Virus injection and optical fiber implantation” 6 weeks prior to the recordings ...
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bioRxiv - Neuroscience 2023Quote: ... Ntsr1-Cre and Rbp4-Cre mice were stereotaxically injected with an inhibitory opsin (AAV1-hSyn-SIO-stGtACR2-FusionRed, 5 x 1011 vg/ml, Addgene) see “Virus injection and optical fiber implantation” ...
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bioRxiv - Neuroscience 2024Quote: ... a subgroup of animals within each rat subline received AAV5-hSyn-DOI-mCherry (7.10¹² vg/mL, Addgene, plasmid number 50459). After injections ...
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bioRxiv - Neuroscience 2024Quote: ... Either AAV-CAG-FLEXFRT-ChR2(H134R)-mCherry (n = 7, 200 nL, 3 × 1012 GC/mL, Serotype: 9; Addgene, MA, USA) to express channelrhodopsin (ChR2 ...
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bioRxiv - Neuroscience 2024Quote: ... Rats then received bilateral infusions of either AAV8-CAMKIIa-hM3D(Gq)-mCherry or AAV8-CaMKIIa-GFP (5×1012 vg/mL for both viruses; Addgene) into the VH (either A/P ...
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bioRxiv - Neuroscience 2024Quote: ... we intracranially injected 400 nL of AAV1-hSyn1-SIO-stGACR2-FusionRed (Addgene 105677-AAV1; titer: 1.0 x 1012 vg/mL) or AAV1-CAG-FLEX-tdTomato (Addgene 28306-AAV1 ...
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bioRxiv - Neuroscience 2024Quote: ... we intracranially injected 400 nL of AAV5-hSyn-FLEx-axon-GCaMP6s (Addgene 112010-AAV5, titer: 2.2 x 1012 vg/mL) into the right BLA at coordinates AP ...
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bioRxiv - Neuroscience 2024Quote: ... and a micropipette containing 100nL of pAAV-hSyn-FLEx-mGFP-2A-Synaptophysin-mRuby (Addgene 71760-AAV1; titer: 9.8×1012vg/mL) was injected unilaterally into the VTA of DAT-IRES-Cre mice.
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bioRxiv - Neuroscience 2024Quote: ... the ReaChR virus was co-injected unilaterally into the LC with AAV9-hSyn-FLEX-jGCaMP8m (3 × 1013 gc/mL, 162378-AAV9 from Addgene) or with AAV9-hSyn-GRABNEm (2-9 × 1013 gc/mL ...
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bioRxiv - Neuroscience 2024Quote: ... animals were injected bilaterally in the mPFC with an anterograde Cre-dependent AAV5-hSyn-DOI-hM3Dq-mCherry (7×10¹² vg/mL, plasmid #44361, Addgene) for RHA rats (hM3Dq-group ...
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bioRxiv - Neuroscience 2024Quote: ... We injected AAVPHP.eB (1012 vg/ml) encoding a red fluorescent protein fused to an opsin (pAAV-TRE3G-BiPOLES-mKate2 (Addgene # 192579)) to label the membrane of cFos+ neurons ...
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bioRxiv - Neuroscience 2020Quote: ... and the retrograde virus carrying Cre (AAV retrograde pmSyn1-EBFP-Cre, 7.6 × 1012 GC/ml) were purchased from Addgene (Watertown, MA).
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bioRxiv - Neuroscience 2020Quote: Transfection for the CD63 overexpression construct was done as described above for siRNA transfection using 2 µg/mL of CD63-pEGFP (Addgene, USA) added for 5 h in Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... AAV2-retro-EF1a-DIO-FLPo (2.6×1013 vg/ml) was packaged by Vigene Biosciences from pAAV-EF1a-DIO-FLPo-WPRE-hGHpA (gift from Li Zhang, Addgene 87306). AAV2-fDIO-mCherry was previously described(42 ...
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bioRxiv - Neuroscience 2022Quote: ... We injected a viral cocktail of AAV5-CamKIIa-C1V1(E122T/E162T)-TS-eYFP-WPRE-hGH (2.5 × 1012 virus molecules/ml; Penn Vector Core, University of Pennsylvania, PA, USA, Addgene number: 35499) in the primary sensory (S1 ...
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Targeting Noradrenergic Neurons of the Locus Coeruleus: A Comparison of Model Systems and StrategiesbioRxiv - Neuroscience 2022Quote: ... Injections of rAAV2/9-Ef1a-DO-DIO-tdTomato-eGFP (2.3 × 1013 gc/ml; kindly gifted from Bernardo Sabatini; AddGene plasmid #37120)34 were performed in TH- and DBH-cre animals to estimate the viral spread ...
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bioRxiv - Neuroscience 2021Quote: ... with serotype and titer (vg/ml) indicated: AAV5-EF1a-DIO-hChR2(H134R)-EYFP (Suppl. Fig. 1A; Penn Vector Core; 7.4 x 1012; Addgene plasmid #20298); AAV5-CMV-EGFP (Suppl ...
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bioRxiv - Neuroscience 2020Quote: ... while eight animals received injections of a non-DREADD expressing viral control AAV5-CaMKIIa-EGFP (titer 4.3×10^12 GC/ml; Addgene, MA, USA), All animals received three viral injections in the anterior cingulate cortex in each hemisphere as follows (skull at +5.0mm to horizontal plane) ...
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bioRxiv - Neuroscience 2020Quote: ... and the control group (n = 10) received injections of AAV5-CaMKIIa-EGFP (titer 4.3×10^12 GC/ml; Addgene, MA, USA). The injection coordinates and volumes for the three injections made into the anterior cingulate cortex were as follows ...
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bioRxiv - Neuroscience 2020Quote: ... the experimental group (n = 12) received injections of AAV5-CaMKIIa-hM4Di-mCherry (titer 4.4×10^12 GC/ml; Addgene, MA, USA;) and the control group (n = 10 ...
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bioRxiv - Neuroscience 2020Quote: ... Drd1Cre(FK150) mice were transduced with AAV2.hSyn.DIO.hM3Dq.mCherry (8.6 x 1012 GC/ml, Addgene viral prep #44361-AAV2, Dr. Bryan Roth)107 or AAV1.CBA.DIO.rM3Ds.mCherry.WPRE (4.3×1013 GC/ml ...
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bioRxiv - Genetics 2021Quote: ... 2 ml (1.5× 106/ml) cells plated in a well of a 6-well dish were transfected with 300 ng of Act::Cas9 (Addgene #62209) and the respective gRNA and repair templates for the 25C6 (75 ng pU6-3-25C6-gRNA1 (DGRC Cat# 1547) ...
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bioRxiv - Neuroscience 2021Quote: ... AAV8-EF1a-DIO-H2B-GFP-2A-OG (10min, 1.54 × 1013 genome units per ml, custom packaged by Vigene Biosciences, Addgene plasmid #74289) was delivered through iontophoresis into target subregions in double transgenic mice of CaMKIIα-Cre ...
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bioRxiv - Neuroscience 2022Quote: ... was injected in the NAcSh of WT mice while AAV9-Syn-Flex-GcAMP6f-eYFP (Addgene, viral titer 2.1 × 1013 vg/mL) was injected in the NAcSh of cre-dependent mice (stereotaxic coordinates from Bregma ...
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bioRxiv - Neuroscience 2022Quote: ... Selective expression of GtACR2-MuGFP-Kv2.1 in preBötC neurons that project to the 7n was obtained by injections of AAV-DIO-GtACR2-MuGFP into the preBötC and the retrograde pseudotyped AAVrg-EF1α-mCherry-IRES-Cre (titre 8x1012 GC/ml; Addgene # 55632-AVVrg) into the 7n.
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bioRxiv - Neuroscience 2022Quote: ... P1 Syngap1+/fl mice were injected with an rAAV9-packaged Cre-GFP expressing virus (pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40: Addgene #105551-AAV9: Titer = 5.66×1013 GC/ml) via the superficial temporal vein (STV ...
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bioRxiv - Systems Biology 2022Quote: ... and added undiluted to K562 cells for a final cell concentration of 3-4 x 105 cells/mL for pJT126-based effector recruitment vectors (Addgene #161926) or 1-2 x 105 cells/mL for pCL040-based inducible protein expression vectors (to be deposited on Addgene ...
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bioRxiv - Neuroscience 2022Quote: ... AAV8-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine (AAV-DIO-hM3Di-mCitrine, 2.3×1013vg/ml, 200 nl/hemisphere, catalog #50454-AAV8, Addgene, Watertown, MA) was delivered bilaterally into the GC of Glp1r-Cre mice at the coordinates above at a depth of -3.25mm DV ...
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bioRxiv - Neuroscience 2023Quote: ... Cre-dependent mCherry under the human synapsin promoter in dHPC (AAV5-hSyn-DIO-mCherry, titer: 1.1 × 1013 vg/ml, Catalog #50459-AAV5, Addgene, Watertown, USA). For optogenetic experiments ...
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bioRxiv - Neuroscience 2023Quote: ... 2014) AAV2-hSyn-DIO-hM4D(Gi)- mCherry vector (titer ≥ 5×10¹² vg/mL) was attained from AddGene (catalog number 44362-AAV2). Rats were anesthetized with 2.5% isoflurane ...
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bioRxiv - Neuroscience 2023Quote: ... The pAAV-hSyn-DIO-mCherry (titer 4.56 e+14 gc/ml) was produced in an in-house viral production facility using the plasmid ordered from Addgene (#50459). The pAAV2/9-pCAG-FLEX-alpha-synuclein(A53T)-3*Myc-WPRE (mutated pαSyn ...
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bioRxiv - Neuroscience 2024Quote: ... The AAV serotype DJ hSyn-DIO-hM4Di-mCherry (6.9 × 1012 gp/mL) was generated by the Fukushima Medical University School of Medicine Viral Vector Core facility with the corresponding plasmid (Addgene #44362).
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bioRxiv - Neuroscience 2023Quote: ... into the AC and a retrograde Cre virus: pENN/AAVrg-hSyn-Cre-WPRE-hGH (1.8E+13 vg/ml, 200nl, Addgene catalog #105553) into the pStr ...
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bioRxiv - Neuroscience 2023Quote: ... We bilaterally injected Cre-dependent DREADD virus (Roth, 2016): AAV5-hSyn-DIO-hM4D(Gi)-mCherry (2.4E+13 vg/ml, 350nl, Addgene catalog # 44362) or AAV5-hSyn-DIO-mCherry (2.6E+13 vg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... was mixed in a 1:10 ratio with AAV5-syn-GCaMP6s and injected into PM and AAV5-Syn-SIO-eOPN3-mScarlet (2−1013 gc/mL; Addgene #125713) was injected into either LP ...
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bioRxiv - Neuroscience 2024Quote: ... We then infused a total of 0.5uL of AAV9-syn-GCaMP7f (titer 2.3×1013 GC/ml, Addgene 104488-AAV9, Watertown, MA) at two separate locations within the craniotomy ...