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Citations for Addgene :
1251 - 1300 of 1882 citations for Vascular Endothelial Cell Growth Factor VEGF Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Human cells used for RNA sequencing were first integrated with Not1-linearized pBABE-neo-hTERT plasmid (Addgene plasmid # 1774) and selected with G418 ...
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bioRxiv - Cell Biology 2021Quote: ... used for creating tetracycline-inducible cell lines were gifts from Eric Campeau (w762-1: Addgene plasmid #26434; http://n2t.net/addgene:26434; RRID: Addgene_26434 ...
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bioRxiv - Cell Biology 2021Quote: ARPE-19 cells were transduced with the lentiviral vector pCW-Cas9 (a gift from Eric Lander & David Sabatini, Addgene plasmid # 50661 ...
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bioRxiv - Microbiology 2020Quote: ... Cells were transfected using Lipofectamine 2000 with plasmids encoding pEGFP-C2 SENP2 (gift from Mary Dasso, Addgene plasmid # 13382) and infected 24 h later ...
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bioRxiv - Microbiology 2020Quote: All virus-like particles were generated in HEK293T cells via calcium phosphate transfection using packaging plasmids psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259) ...
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bioRxiv - Cancer Biology 2020Quote: HEK293T cells were transfected with packaging plasmid psPAX2 and envelope plasmid pMD2.G (both gifts from Didier Trono, Addgene plasmid # 12260 ...
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bioRxiv - Cancer Biology 2020Quote: MOLM-13-Cas9 cells were first transduced with a luciferase expressing cassette in Lenti-luciferase-P2A-Neo (Addgene #105621) vector ...
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bioRxiv - Cancer Biology 2021Quote: ... The 293 cells were seeded in 10-cm-diameter dishes and transfected with pCMV-dR8.2 dvpr (Addgene plasmid #8455), pCMV-VSV-G (Addgene plasmid #8454 ...
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bioRxiv - Biophysics 2022Quote: Escherichia coli NiCo21 (DE3) competent cells were transformed with the plasmid encoding sumo-tag fused DiCas7-11 (Addgene: 172503). A single colony was picked and transferred to 100mL LB media containing 50 μg/mL ampicillin and grown for 12 hours at 37°C before inoculation into 1L LB culture ...
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bioRxiv - Microbiology 2022Quote: ... Cells were seeded in 96-well plates and transfected 24h after with pMXs GFP-LC3-RFP (#117413, Addgene, MA) or with Polyinosinic-polycytidylic acid sodium salt ...
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bioRxiv - Immunology 2022Quote: ... cells were transfected with 1.0 μg of pLV-PTB or pLV-CTRL with 0.8 μg psPAX2 (Addgene plasmid 12260) and 0.2 μg of pCI-VSVG (Addgene plasmid 1733 ...
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bioRxiv - Cancer Biology 2022Quote: ... HEK293T cells (Dharmacon) were transfected with the Ass1 targeting lentiCRISPRv2 vectors and the lentiviral packing plasmids psPAX2 (Addgene: #12260) and pMD2.G (Addgene ...
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bioRxiv - Cancer Biology 2022Quote: ... T98GEmpty and T98GcGAS cell lines were stably transfected with sfGFP-N1 (gift from Michael Davidson & Geoffrey Waldo; Addgene #54737) using phosphate calcium ...
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bioRxiv - Cancer Biology 2022Quote: ... The PDE6D scan library contains 116 unique sgRNA was packaged by HEK293 cells (ATCC) co-transfected with psPAX2 (Addgene) and pMD2.G (Addgene ...
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bioRxiv - Cell Biology 2021Quote: HEK293 T-REx SNAPf-GLP-1R cells were co-transfected with an AP2-HA plasmid (μ2-HA-WT; Addgene plasmid # 32752 ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK293T cells were transfected with the relevant vectors and the second-generation packaging plasmids ΔR8.2 and Vsv-G (Addgene). Virus-containing supernatants were collected 48 h later and added with Polybrene to AML cells pre-seeded at ∼5 × 105/well in 24-well plates (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2021Quote: ... These pLKO.1 constructions were transfected in the 293T packaging cell line along with pMD2.G and pCMV-dR8.91 vectors (Addgene), following a typical Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... A549-ACE2-TMPRSS2 and Huh7-TMPRSS2 stable cell lines were generated using commercial lentivirus (Addgene, catalog no. 154982-LV) and selected using 10 μg/ml blasticidin (InvivoGen ...
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bioRxiv - Cancer Biology 2022Quote: Polyclonal SK-N-DZ cells constitutively expressing the CRISPR activation machinery were engineered by transducing wild-type cells with lentiviral particles carrying a dCas9-VP64 (lenti dCas9VP64_Blast, Addgene plasmid #61425 was a gift from Feng Zhang ...
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bioRxiv - Cancer Biology 2022Quote: ... and the resulting pQCXIN-PD-L1-IRES-Thy1.1 plasmid was transfected into HEK293T cells along with pCMV-VSV-G (a gift from Bob Weinberg; Addgene plasmid 8454 ...
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bioRxiv - Immunology 2019Quote: ... 2×106 HEK293T cells stably expressing FLAG-tagged Hem1 were transiently transfected with 2 μg myc-Rictor plasmid (Addgene). Control HEK293T cells were transfected with myc-Rictor or 10 ng GFP-3xFLAG as described ...
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bioRxiv - Systems Biology 2019Quote: MCF10A cells were transduced with nuclear RFP-expressing lentivirus (LV-RFP, a gift from Elaine Fuchs, Addgene plasmid #26001) and treated under conditions listed in Table 1 ...
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bioRxiv - Molecular Biology 2021Quote: ... mouse Sp7 and GFP lentiviruses were generated by transfecting HEK293T cells with a blasticidin resistance backbone (Addgene, plasmid 26655) along with psPAX2 and MD2.G ...
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bioRxiv - Molecular Biology 2021Quote: The cell cycle reporter vector pCSII-EF-miRFP709-hCdt(1/100) was a kind gift from Vladislav Verkhusha (Addgene plasmid # 80007 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Lentiviral particles were produced in 293T HEK cells by co-transfection of the lentiviral pCW57.1_Gata6 plasmid with VSV-G (pMD2.G, Addgene #12259) and helper plasmid (psPAX2 ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293T cells were transfected with plasmid encoding eSpCas9(1.1) and sgRNAs targeting CCT5 and ATP1A1 (modified from Addgene #86613) along with linear dsDNAs to act as HDR templates by which to insert 3xFLAG/Spytag at the C-terminus of CCT5 ...
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bioRxiv - Biochemistry 2021Quote: ... we transfected HEK293T cells with Cas9 and sgRNA targeting the gene encoding for the CCT5 subunit (Addgene eSpCas9(1.1) vector ...
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bioRxiv - Genetics 2020Quote: ... We generated cell lines expressing KRAB-dCas9-IRES-BFP under the control of a doxycycline-inducible promoter (Addgene #85449) and the reverse tetracycline transactivator (rtTA ...
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bioRxiv - Cell Biology 2020Quote: ... the LoxStopLox cassette was removed by transfecting the cells with LV-Cre (from Inder Verma via Addgene, plasmid # 12106) using the previously described transfection protocol ...
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bioRxiv - Cell Biology 2020Quote: The plasmid carrying Cas9/sgRNA was co-transfected into HEK293FT cells with the lentiviral packaging vectors psPAX2 (Addgene #12260) and VSV-g (Addgene #8454 ...
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bioRxiv - Immunology 2021Quote: ... Virus was harvested from GP-2 cells transfected with SINV vectors and VSV-G (pMD2.G, Addgene plasmid #12259) and grown in DMEM supplemented with 30% FBS and 2mM glutamine ...
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bioRxiv - Cancer Biology 2021Quote: ... the adherent cell cultures were cultured in the presence of lentiviral particles containing ΔLTR flanked CMV:tdTomato-blasticidin (Addgene#106173) and 8µg/mL polybrene (Sigma ...
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bioRxiv - Cell Biology 2019Quote: Lis1 shRNA Lentiviral particles were prepared in HEK293T cells and RAW 264.7 macrophages were transduced with these particles using protocol as recommended by Addgene. Transductant cells were selected on Puromycin (2.5 µg/mL) ...
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bioRxiv - Neuroscience 2022Quote: ... chr.14 5’-AAGAAAGAAACTTGGCATAG-CAG 14:56,271,668-56,271,690) was assessed in transfected HeLa cells using TurboRFP open reading frame reconstitution reporter plasmid (pAR-TurboRFP; Addgene #60021) as described previously (Kasparek et al. ...
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bioRxiv - Cell Biology 2022Quote: Lentiviral supernatants were produced in HEK293Lx cells following transfection with 3rd generation packaging plasmids and either pLKOshp53 (Addgene #19119), pLKOshScr (Addgene #1864) ...
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bioRxiv - Bioengineering 2022Quote: ... To generate H2B-iRFP lentiviral particles HEK 293T cells were plated in a 6-well plate (7×105 cells per well) and transiently transfected with 1.5 μg of pLenti-pGK-DEST-H2B-iRFP vector (Addgene # 90237 ...
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bioRxiv - Cell Biology 2022Quote: Lentivirus was generated by co-transfecting HEK293T cells with two packaging plasmids (pCMV-VSV-G and delta8.9, Addgene #8454) and the desired transfer plasmid using TransIT-293 transfection reagent (Mirus) ...
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bioRxiv - Cell Biology 2022Quote: ... hTERT-RPE1 Plk1 FRET Sensor cells were prepared by transfecting Plk1-FRET sensor c-jun substrate plasmid37 (Addgene: 45203) in hTERT-RPE1 cells using X-tremeGENE™ 9 (Merck ...
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bioRxiv - Cell Biology 2022Quote: ... Lentiviral particles were produced in 293T cells by using psPAX2 and pCMV-VSV-G packaging plasmids (Addgene 12260, 8454). Viral supernatant was collected after 48h ...
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bioRxiv - Cancer Biology 2022Quote: ... Retroviral particles were produced in 293T cells by transfecting pCG-gag-pol and pCMV-VSV-G packaging plasmids (Addgene) together with the corresponding retroviral plasmid ...
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bioRxiv - Molecular Biology 2023Quote: CRISPR mediated mutagenesis of target genes was performed by transducing K562 cells stably expressing Cas9 (lentiCas9-Blast, Addgene #52962) with lentivirus expressing sgRNAs (lentiGuide-Puro ...
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bioRxiv - Neuroscience 2022Quote: ... N2a cells were co-transfected with the wild-type or FeRIC channels and GCaMP6 (GCaMP6 medium, Addgene cat.40754) or YFP-H148Q ...
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bioRxiv - Microbiology 2022Quote: ... Lentiviruses containing shRNA or sgRNA were produced using 293FT cells with packaging constructs pCMV-VSVG and pCMV-Delta 8.2 (Addgene). The lentiviruses were collected 48 hrs post transfection and concentrated by ultracentrifugation at 25,000 rpm for 2 hrs ...
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bioRxiv - Cancer Biology 2022Quote: ... 4T1 and EMT6.5 cancer cells were transfected with the mammalian expression lentiviral vector pLKO.3 Thy1.1 (Addgene plasmid #14749) containing the surface protein Thy1.1 as a reporter protein ...
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bioRxiv - Bioengineering 2022Quote: ... Lentivirus was produced using calcium phosphate co-transfection of HEK293T cells with the psPAx2 plasmid (packaging vector, Addgene #12260), pMd2g plasmid (VSVG envelope ...
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bioRxiv - Genomics 2024Quote: Lentivirus was produced from transfected HEK293T cells with packaging vectors (pMD2.G #12259, Addgene, and pCMV-dR8.91, Trono Lab) following the manufacturers protocol (#MIR6605 ...
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bioRxiv - Molecular Biology 2024Quote: ... Parental HEK293 cells were co-transfected with 1.0 µg each of PBKS-Cas9-2A-eGFP plasmid (Addgene plasmid #68371) and plasmid encoding for gRNA ...
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bioRxiv - Cancer Biology 2024Quote: ... Lenti-U6BC-sgDbl/Cre vectors were transfected as a pool into 293T cells with pCMV-VSV-G (Addgene #8454) envelope plasmid and pCMV-dR8.2 dvpr (Addgene #8455 ...
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bioRxiv - Microbiology 2024Quote: ... pseudovirus constructs (PV) have been developed by three-plasmid co-transfection in HEK293 cells using lentiviral backbone (Addgene # 8455), firefly luciferase reporter (Addgene #170674 ...
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bioRxiv - Immunology 2024Quote: Pseudo-lentiviral particles containing sCD177 construct were generated using 293T cells and packaging vectors pMD2.G and pCMV-dR8.74psPAX2 (Addgene). Pseudo-lentiviral particles were transduced into the FreeStyle 293-F cells (ThermoFisher Scientific) ...